DIAGNOSIS LABORATORIUM Laboratorium Mikrobiologi FK UKM

INFEKSI JAMUR
Fanny Rahardja
SPECIMEN COLLECTION AND TRANSPORT

Transport medium : none

Sterile container Skin scrapping :

(+ penicillin, chloramphenicol, streptomycin ) envelope, petri dish

Direct examination Culture medium

Presumtive diagnosis

Select appropiate culture medium

 Direct examination – wet mount

KOH preparation
debris
fungal elements

India ink preparation

Fluorescent
Calcofluor White Stain microscope
 Sabouraud Dextrose Agar
Primary recovery medium

Sabouraud Dextrose Agar

+ penicillin, streptmycin, gentamicyn
Incubation
30°C and 35°C
Culture medium
30 days

Sabhi agar (SDA+BHI) :

fastidious /slow growing fungi
 MOLDS Culture medium – Macroscopic observation

surface

Appearance of the growth Rate of growth

Reverse

Top of the colony

Colony pigmentation Dimorphic growth
Diffused into the medium Underside the colony

Growth on media
containing antifungal agents
 MOLDS Culture medium – Macroscopic observation

surface

Appearance of the growth

Reverse

Texture :
cottony, velvety, wolly

Topography :
Verrucose, rugose, umbonate
Appearance of the growth Culture medium – Macroscopic observation
surface Reverse

Trychophyton Trychophyton
rubrum tonsurang

Trychophyton
verrucosum
Epidermophyton
flocossum
 MOLDS Culture medium – Macroscopic observation
Topography
 MOLDS Culture medium – Macroscopic observation
Texture
cottony

Glabrous

Granular

Velvety
MOLDS Culture medium – Microscopic observation

Fungal Tease Adhesive tape Slide culture

Preparation technique technique

100 x
400 x
MOLDS Culture medium – Microscopic observation

Fungal Tease Preparation

MOLDS Culture medium – Microscopic observation

Adhesive tape technique

 MOLDS Culture medium – Microscopic observation

Slide culture technique

 Culture medium – Microscopic observation
YEAST

Cornmeal agar

Cryptococcus
Urea agar Tricosporon

Wet mount

Biochemical test
Germ tube test Carbohydrate assimiliation
Candida albicans
 Culture medium – Microscopic observation

Hyphae : Vegetative spore :

Coenocytic , septate Arthrospore, chlamydospores, blastospore
Colour of hyphae
Aerial spore :
Macroconidia, microconidia, sporangiospore

Sexual spore :
Ascospore, zygospore, basidiospore
 Sexual spore :
Ascospore, zygospore, basidiospore

Zygospore Ascospore Basidiospore

 KOH 10%
Skin scrapping

Trychophyton rubrum
Slender clavate microconidia Cigar-shaped macroconidia
 “Spaghetti with meatballs”

KOH 10%

Skin scrapping

*Short angular hyphae,

Malassezia furfur *Cluster thick-wall round budding yeast-like cell

inkubasi

Modified Dixon Agar

 KOH 10%
Skin scrapping

onichomycoses

Epidermophyton floccosum

SDA, 26 °C, 28 days

 KOH 10%

Skin scrapping

Microsporum canis

Clavate /pyriform microconidia spindle-shaped Macroconidia with 5-15 cells,

verrucose, thick-walled and often have a
+ Rice grain medium terminal knob.
Thank U