Tizon, Sophia Marie G

BSMT 3-2
CLINICAL CHEMISTRY-LAB

Exercise 11: Inorganic Phosphorus-Availability Ends

1. List the different methods used in the assessment of patient inorganic phosphorus. Give
the principles for each test.

Method of Fiske and Subarrow.

When ammonium molybdate is introduced to an acid solution that already contains serum
protein free filtrate, phosphomolybdic acid is produced as a byproduct of the reaction. In
order to transform phosphomolybdic acid into phosphomolybdic acid with blue
molybdenum so that it may be measured photometrically, para–amino naphthol sulfonic
acid reagent must be added.

When phosphorus combines with molybdic acid, a compound known as phosphor

molybdic acid is produced. The reduction of phosphorus molybdic acid results in the
production of a deep color called molybdenum blue, which is a mixture of several other,
less significant molybdenum acids. Spectrophotometric analysis at 660 nm is used in the
approach developed by Fiske and Subbarow. This analysis is used to determine the blue
color.

2. Discuss the difference between the method that uses a reducing substance and
the method that does not use a reducing substance.

In a chemical reaction involving oxidation and reduction, one of the reactants is a reducing agent,
which, by giving off electrons, causes the concentration of the other reactant to drop. Procedures
that involve the use of a reducing drug have this end goal in mind. The reduction process will be
unsuccessful if the reducing agent does not successfully transfer electrons to the other
components that are involved in the reaction. On the other side, procedures that don't need a
reducing substance either have sugar that doesn't reduce or don't have a free aldehyde or ketone,
which means they don't use either of those substances. When a reducing substance is chemically
changed, it may contribute electrons to another molecule, which results in alterations to the color
and flavor of the dish.

3. What is the clinical significance of inorganic phosphorus?

Phosphorus toxicity can manifest itself in a number of ways, including kidney illness and
hypoparathyroidism, which occurs when your parathyroid gland does not generate enough of the
hormone parathyroid.

• An excessive amount of vitamin D in the body

• A diet that contains an excessive amount of phosphate
• Diabetic ketoacidosis, a life-threatening complication of diabetes

Phosphorus levels that are too low Hyperparathyroidism is a condition that develops when the
parathyroid gland produces an excessive amount of parathyroid hormone.

• Malnutrition \s
• Alcoholism
• Osteomalacia is a disorder that results in the bones becoming softer and more misshapen.
The problem is a lack of vitamin D in the diet. This condition in children is known as rickets,
and it can be quite dangerous.

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