VegaSys Service Manual Rev.01

VEGASYS
Service Manual
Via E. Barsanti 17/A

00012 Guidonia – ROME (ITALY)
www.ams-analyzers.com
INDEX
TABLE OF CONTENTS
Chapter 01 INSTALLATION
Chapter 02 SYSTEM DESCRIPTION
Chapter 03 ELECTRICAL SCHEMES AND DRAWINGS
Chapter 04 DIAGNOSTIC PROGRAM
Chapter 05 SETTINGS & ADJUSTMENTS
Chapter 06 MAINTENANCE
Chapter 07 HOST COMMUNICATION
Chapter 08 TROUBLESHOOTING GUIDE
VegaSys Service Manual Rev.01 15 March 2009 Page 1

Chapter 01 - INSTALLATION
CHAPTER 01
- INSTALLATION -
INDEX
1.1 UNPACKING……........................................................................................................... 2
1.2 INSTRUMENT INSTALLATION... ............................................................................... 4
1.2.1 INSTALLATION SITE……............................................................................................ 4
1.2.2 ELECTRICAL POWER SUPPLY ................................................................................... 4
1.2.3 ACCESSORIES CONNECTIONS .................................................................................. 5
1.2.3.1 Power Supply........................................................................................................... ..... 5
1.2.3.2 Instrument-Computer connection ................................................................................. .5
1.2.3.3 Liquid connections…………….............................…………………………………....5
1.2.4 LABELS AND SYMBOLS..............................................................................................6
1.2.5 WARRANTY...…….…………….……………………………………………………...7
1.2.6 REGULATORY COMPLIANCE...………………...……..………..……………..….…7
1.2.7 LIMITATION OF USE..........................................................................................……....7

1.1 UNPACKING
The Vegasys is packed and delivered in one wooden crate/box containing the instrument and
the its accessories.
The packing modalities have been expressly designed to insure maximum protection of the
contents during shipping and handling. Nevertheless it is important to carefully check the
crate/box upon delivery in order to ascertain its integrity.
Special attention should be dedicated to examining the color of the ShockWatch ® glued to
the crate: it must be white. A ‘red’ ShockWatch ® indicates that the crate has experienced some
sort of mishandling (shock) during transport and/or delivery. This fact must be recorded by the
courier on the delivery note, as must any and all visible external damage to the packing crate
(e.g.: holes, dents, rips or tears, water marks, etc.) evident at the delivery moment. This serves to
simplify matters in the event of any future claims for damages.
NOTE: THE CARRIER ASSUMES TOTAL RESPONSIBILITY FOR THE CRATE AND/OR
BOX AND THEIR CONTENTS ONCE THE GOODS HAVE LEFT THE FACTORY
UNTIL THE MOMENT THEY ARE DELIVERED AT DESTINATION. THE CARRIER
IS RESPONSIBLE FOR ANY AND ALL DAMAGES ARISING DURING HANDLING,
STORAGE AND TRANSPORTATION. ANY AND ALL DAMAGE CLAIMS MUST BE
FILED AGAINST CARRIER IMMEDIATELY UPON DISCOVERY.
Upon consignment of the crate, take out the

delivery note and make sure that all the items S/N
on the packing list are included in the crate
P/N
and that they are undamaged. Check that the
serial number on the delivery note/packing list 100 - 230V~ 47 - 63Hz 180W
corresponds to that impressed on the plate on Analyzer
Medical
the left side of the instrument. Rome-Italy

System
IVD
Open the crate/box from the top and very

carefully take out the instrument and the
accessories.
MAKE SURE THAT UNPACKING IS CARRIED OUT BY TWO PEOPLE.
Do not discard the delivery crate/box or the packing material until the correct functioning of
the instrument has been checked.

Before connecting the "Vegasys", remove the protective packing material of the sampling and
aspiration arms.
Warning: in case it is necessary to repack the delivered unit, the following procedure must be
carefully followed:
Reposition the protective packing material of the sampling and aspiration arm.
Tape down (using masking tape if possible) the main cover of the instrument.
Fill the empty spaces around the accessories packed in the crate using “pluriball” or other
suitable packing material.
In case the repacking is done after clinical diagnostic use of the instrument, it is necessary to
first perform the decontamination procedure as outlined in Chapter 6 of this manual.
Ambient-climatic conditions for transportation, warehousing and storing the instrument

inside its packing crate:
- Temperature: between –20° and +70°C
- Humidity: 10% ÷ 90%
- Warehoused: INDOOR
- Maximum length of warehousing: 6 months (after longer period, preventive maintenance
regarding certain components is required - e.g.: membranes).

1.2 INSTRUMENT INSTALLATION

The Vegasys instrument must be installed only by authorized, qualified technical personnel.
During instrument installation, the system will be checked once again to assure proper
functioning. The Vegasys is for professional use only; the operators (in addition to the academic
qualifications and state certification required to meet government regulations) must be trained to
proper use of the instrument including ordinary maintenance practices. Maintenance activities
are described in Chapter 6 of this manual.
In order to avoid risks of potential biological hazards, it is absolutely necessary to comply
with laboratory good practices regarding the handling of biological samples, consumables and
waste (solid and liquid).
The Vegasys is a complex system and it is of utmost importance to correctly follow the
installation procedure in order to guarantee optimum levels of performance. In the event that the
indications reported herein and/or relevant safety norms are not followed, AMS does not
guarantee proper instrument functioning. Furthermore, user safety may be placed at risk.
1.2.1 INSTALLATION SITE

Make sure that the Vegasys is not installed under direct sunlight, subject to drafts, dust or
strong magnetic fields. Observe the requirements below reported for the installation site:
USE Indoors and dry

POLLUTION DEGREE 2
ISOLATION CLASS I
INSTALLATION CATEGORY II
TEMPERATURE OF USE between 18°C and 30°C
HUMIDITY 20% ÷ 85%
ALTITUDE Maximum: 3000 m
Counter-top or table with minimum surface area of 70
x 60 cm. Stable surface with no vibrations. Position the
PLACEMENT instrument in such a manner that it can be easily
disconnected.
Leave a space of at least 10 cm around the instrument
VENTILATION in order to allow air circulation. Make sure that the fan
is not obstructed.
1.2.2 ELECTRICAL POWER SUPPLY

The electrical power tension/voltage for the instrument is indicated on the right-hand side of
the instrument (see the label at Page 2). This same information is also reported on the label
located above the input line socket. Make sure that power supply used conforms to that here-
indicated and that it is properly grounded:
VOLTAGE 100 : 230Vac 47/63Hz ±10%

PLEASE NOTE: IT IS IMPORTANT THAT THE LABORATORY’S ELECTRICAL POWER SOURCE BE

STABLE. IF SAID STABILITY CANNOT BE GUARANTEED, THE USE OF THE
FOLLOWING DEVICES IS STRONGLY SUGGESTED:
⇒ ELECTRONIC STABILIZER
Used to stabilize the power source voltage in the laboratory. Any stabilizer available on
the market can be used (power not less than 0.5 kW).
⇒ NO-BREAK MODULE (UPS - Uninterrupted Power Supply)

This module stabilizes the power voltage and supply power to the instrument in case of a
power failure.
1.2.3 ACCESSORIES CONNECTION

1.2.3.1 Power supply
Plug the power supply cable that is provided to connect the instrument to the power source.
1.2.3.2 Instrument-Computer connection

Connect the Vegasys to the Personal Computer via a RS232 or USB standard serial cable.
1.2.3.3 Liquid connections

Connect the:
¾ loading bottle for the wash solution to be used during analytical routine;
¾ discharging bottle for the waste liquid.

1.2.4 LABELS AND SYMBOLS
The following WARNING label can be found on the left side of the instrument:
PLEASE NOTE: NEVER OPEN THE INSTRUMENT WITHOUT HAVING FIRST TURNED IT OFF AND
DISCONNECTED IT FROM ITS POWER SOURCE (UNPLUG THE POWER SUPPLY
CABLE). CAREFULLY FOLLOW THE CLEANING AND MAINTENANCE PROCEDURES
AS OUTLINED IN CHAPTER 6 OF THIS MANUAL.
CAUTION! READ INSTRUCTIONS IN THE OPERATOR MANUAL
EARTH GROUND
BIOLOGICAL HAZARD
(Placed next to the Samples Plate and the waste discharge red fitting)
Wear proper protective clothing/equipment and handle all biological
samples, consumables and waste liquids produced during analysis with
extreme care. The disposal of the used consumables and the waste liquids
produced must conform to all applicable government laws and regulations.
Furthermore, to prevent overflow of the discharge tanks, make sure to
periodically check the level of the waste liquids collected.
MANUFACTURING COMPANY
This symbol is placed on the S/N plate next to the name of the
manufacturer.
DISPOSABLE WARNING
This symbol is placed on the S/N plate and means that the instrument and
the parts are not to be disposed as ordinary household waste/garbage. They
must be disposed according to applicable government regulations regarding
electrical and electronic equipment (Directive 2002/96/EC WEEE).

1.2.5 WARRANTY
AMS guarantees the replacement of all defective components and/or materials for a period up
to 14 (fourteen) months from the date of invoice. Warranty, as for all Technical Assistance in
general, is intended as provided ex works, Rome, Italy.
Warranty does not cover consumables or those parts of the instrument in direct contact with
liquids. A list of the parts excluded from warranty is provided in the table below.
Moreover, the warranty is not valid in case damages to the VegaSys arise from:
¾ improper use of the instrument (or use in any manner not in conformance with
instructions provided by the Manufacturer or the Seller);
¾ mishandling and/or transportation errors;
¾ insufficient (or lack of) ordinary preventive maintenance by the User.
Any damage arising from mishandling or caused during transport must be immediately
reported to the Shipping Company upon delivery of the goods.
Æ LIST OF CONSUMABLES AND ACCESSORIES EXCLUDED FROM WARRANTY:
Description
Reagents containers
Samples cups
Reaction Racks
Liquid bottles
Tubes
Probes
Lamp
Interferential filters
1.2.6 REGULATORY COMPLIANCE
The Vegasys complies with:

• European Directive 98/79/CE for In-Vitro Diagnostic Devices
Compliance is indicated by the here-illustrated symbols placed on the Serial Number plate:
1.2.7 LIMITATION OF USE

The Vegasys can not be used by blind operators because the user interface with the system
requires a monitor. Furthermore the Vegasys must be used with particular caution by color blind
operators because the graphic interface displays different colors with different meaning.

Chapter 02 - General Description
CHAPTER 02
- GENERAL DESCRIPTION -
INDEX
2.1 SYSTEM DESCRIPTION ............................................................................................2
2.2 MAIN COMPONENTS OF THE ANALYZER ..................................................2
2.2.1 ANALYTICAL PLATE ....................................................................................................2
2.2.2 SAMPLING SYSTEM .....................................................................................................3
2.2.3 SAMPLING ARM ............................................................................................................3
2.2.4 ASPIRATION ARM .........................................................................................................5
2.2.5 PROBE CLEANER ..........................................................................................................5
2.2.6 WASHING WELL............................................................................................................5
2.2.7 INCUBATOR....................................................................................................................5
2.2.8 PERISTALTIC PUMP......................................................................................................5
2.2.9 PHOTOMETRIC SYSTEM..............................................................................................6
2.2.10 MICRO-FLOW CELL .....................................................................................................7
2.3 COMPUTER AND SOFTWARE FEATURES ......................................................8
2.4 DIMENSIONS AND WEIGHT .................................................................................8
2.5 TECHNICAL AND OPERATIVE FEATURES ...................................................9

2.1 SYSTEM DESCRIPTION

The VegaSys is an Open, Automatic Counter Top, Batch Analyzer for Clinical Laboratory. It
runs immuno-turbidimetric, clinical chemistry analysis using single or double reagent
methodologies.
The first time the system is used for laboratory analyses, the user must configure the system
basing on the specific needs of the laboratory. The user must program the chemistry methods-
parameters, the reagents racks, enter the expected value ranges for samples, calibrators and
controls.
NOTE: the VegaSys is an open system that allows configuration with different reagents
selected by the customer in order to fit his needs.
In order to assure the analytical performances of the system “instrument + reagents”, it is
responsibility of the laboratory staff to use reagents, controls and calibrators validated on the
VegaSys or, in alternative, to qualify other reagents, controls and calibrators in compliance
with the applicable regulations.
The daily routine analyses will be carried out according to patient samples arrival in a
sequential order. The work list is organized using loading rack holding up to 10 patient
samples.
2.2 MAIN COMPONENTS OF THE ANALYZER

2.2.1 ANALYTICAL PLATE
The analytical plate (Fig. 1 and Fig. 2) is the main movable part of the analyzer. On this plate
are placed:
⇒ six racks of 19 reaction cells for a total of 114 cells;
⇒ one rack for 6 standards and 2 controls;
⇒ four racks for a total of 40 samples;
⇒ one removable reagent tray for 18 reagent containers (the reagent tray can be easily
removed for refrigeration).
The maximum volume capacity of the reaction cells, sample cups and reagent containers for
the analytical plate are as follows:
¾ Volume of reaction cell: 1.2 ml;
¾ Volume of sample cup: 0.8 ml;
¾ Volume of reagent containers: 25 ml.
The basic operating cycle takes 18 seconds.

The reactions take place at 37°C. This temperature is maintained constant by a controlled
heading unit placed around the reaction plate.

Fig. 1 – Analytical plate
2.2.2 SAMPLING SYSTEM

The sampling system consists of a highly precise diluter controlled by a microprocessor and
has the following specifications:
Diluter volume 1000 µl
Accuracy > 99 %
Sample volume from 3 to 99 µl with 1 µl increment
Reagent volume from 5 to 500 µl with 1 µl increment
Minimum reaction volume 450 µl
The recommended minimum sampling volume for analysis is 5 µl and for reagent volume is
400 µl. There is also an automatic washing cycle in between tests to avoid the risk of cross-
contamination.
2.2.3 SAMPLING ARM

The sampling arm (Fig. 2) contains the sampling probe that aspirates the reagent and the
sample to be analyzed and transfers them into the reaction cell for incubation.

Washing well
Sampling arm
Aspiration arm Analytical plate
Fig. 2 – Sampling arm and aspiration arm
Fig. 3 – Probe cleaner

2.2.4 ASPIRATION ARM

The aspiration arm (Fig. 2) transfers the reaction mixture from the reaction cell to the micro-
flow cell of the photometric system for measurement.
2.2.5 PROBE CLEANER

Located at the base of the sampling arm, the probe cleaner (Fig. 3) consists of a number
(approximately 16) of circular filter paper strips mounted on a holder. This cleans the sampling
probe (Fig. 3) before and after the aspiration and dispensing of reagents.
2.2.6 WASHING WELL

The washing well (Fig. 2) is the place where the washing of the sampling probe takes place
to avoid cross-contamination between sampling.
2.2.7 INCUBATOR
The incubation temperature of the reaction cell is fixed at 37°C ( ± 0,2°C ) to ensure proper
incubation for all samples.
2.2.8 PERISTALTIC PUMP

The peristaltic pump (Fig. 4) operates while the aspiration arm transfers the contents of the
reaction cells into the micro-flow cell.
Fig. 4 – Peristaltic pump

The characteristics of the peristaltic pump are as follows:

Pump operation by stepper motor
Nominal volume of aspiration from 300 to 1000 µl
Aspiration rate 400 µl per 1.5 sec
2.2.9 PHOTOMETRIC SYSTEM

The photometer system (Fig. 5) consists of a narrow bandwidth interferential filters of
340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 578 nm, 620 nm. The other characteristics of the
optical system are as follows:
Absorbance range 0.001 - 2.500 O.D.
Resolution 0.001 O.D.
Accuracy ± 0.001 O.D.
Band-pass ± 2 nm at 340 & 405 nm; 8 nm for visible range
Light source 6V-10W halogen lamp
Detector Silicon photodiode
Zeroing Automatic, computer controlled
Fig. 5 – Photometer

2.2.10 MICRO-FLOW CELL

The measuring cell of the instrument is a micro-flow cell (Fig. 4, Fig. 5 and Fig. 6) with the
following characteristics:
Light path 10 mm
Cuvette body stainless steel & quartz
Inlet tube Teflon
Cuvette volume 50 µl
Temperature 37°C - Peltier effect
Temperature accuracy ± 0.1°C
Carry over 2% nominal for total volume of 450 - 500 µl of solution
Aspiration computer controlled
Fig. 6 – Micro-flow cell

2.3 COMPUTER AND SOFTWARE FEATURES

Minimum requirements:
¾ Type: IBM Compatible;
¾ CPU: Pentium III 300 MHz - 512 Kb Cache or plus;
¾ Memory: RAM 256 Mb or plus;
¾ Hard Disk: 20 Gb or plus;
¾ CD ROM: 24X;
¾ Video Memory: 4 Mb;
¾ Monitor: SVGA 15’’ color – Resolution: 1024 x 768 pixels;
minimum number of colors: 65.536 (16 bit);
¾ Printer: Windows compatible;
¾ Keyboard & Mouse: Standard Keyboard 101 keys – PS2;
¾ Interface: RS 232 serial or USB port;
¾ Software: Multitasking WINDOWS XP;
¾ Settings: disable all the energy saving options; disable the screen saver.
NOTE: even though most computers declare the same technical and operative characteristics,
some of these could have different hardware installed.
This could cause problems to the VegaSys software while running tests. Therefore, if
the PC is bought separately/locally, it is strongly recommended to test the system
before proceeding with the installation at customer laboratories.
Consequently, AMS denies any responsibility for software problems that are due to
computer not previously tested with the instruments.
2.4 DIMENSIONS AND WEIGHT
⇒ Dimensions:
Height: 32 cm
Depth: 45 cm
Length: 50 cm
⇒ Weight: 22 Kg

2.5 TECHNICAL AND OPERATIVE FEATURES

Resume of main technical features
⇒ Assay Type: End Point, Differential, Initial Rate (or Fixed Time), Kinetics and
Bi-chromatics methods.
⇒ Test Selection Mode: Batch, Profile.
⇒ Throughput: up to 200 colorimetric test/hr.
⇒ Working Temperature: 37° C.
⇒ On Line Reagents: 18 Reagents (25 ml).
⇒ Standards & Controls: one rack for 6 standards and 2 controls.
⇒ Samples Loading: four racks for a total of 40 samples.
⇒ Minimum Reaction Volume: 450 µl.
⇒ Sampling Arm: provides to aspirate reagent and sample; automatic probe washing.
⇒ Aspiration Arm: transfers the reaction mixture from the reaction cell to the micro-
flow cell of the photometric system of measurement.
⇒ Diluter: integrated module with the following specifications:

Sample volume: 3.0 µl ÷ 99 µl (1 µl incr.);
Reagent volume: 5.0 µl ÷ 500 µl (1 µl incr.).
⇒ Reaction Plate: six sectors of 19 reaction cuvettes for a total of 114 cuvettes.
⇒ Optic System:
Photometer: multi wavelength photometer with narrow band interferential filters;
Wavelength: 340 nm, 405 nm, 492 nm, 510 nm, 546 nm, 578 nm and 620 nm;
Light source: halogen lamp 6V/10 W;
Linear range: 0.001 ÷ 2.500 Abs;
Resolution: 0.001 Abs;
Detector: silicon photodiode.
⇒ Optical Path: 10 mm.

Chapter 03 – ELECTRICAL SCHEMES AND DRAWINGS
CHAPTER 03
- ELECTRICAL SCHEMES AND DRAWINGS -
INDEX
Hydraulic Diagram ……………………………...…………. SI-16-02329-XX Rev. A
Analytical Control Board (connections scheme)……………... SC-30-02314-00 Rev. 0
VegaSys Service Manual Rev.01 15 March 2009 Page I

Analytical Control Board
SC 30-02314-00 0
CHAPTER 04 – DIAGNOSTIC PROGRAM
CHAPTER 04
- DIAGNOSTIC PROGRAM -
INDEX
4 DIAGNOSTIC PROGRAM ........................................................................................................ 2

4.1 “SAMPLING ARM/PLATE SETTINGS” FOLDER ............................................................... 6
4.1.1 “GENERAL RESET” AREA ........................................................................................... 7
4.1.2 “WASHING WELL” AREA ............................................................................................ 7
4.1.3 “REAGENTS” AREA ...................................................................................................... 8
4.1.4 “SAMPLES” AREA ......................................................................................................... 9
4.1.5 “CUVETTE-SAMPLING” AREA ................................................................................. 10
4.1.6 “CUVETTE-ASPIRATION” AREA .............................................................................. 10
4.1.7 “NO3” AREA................................................................................................................... 11
4.1.8 “FREE COVER” AREA .................................................................................................. 11
4.1.9 “ALL POSITIONS” AREA ............................................................................................. 11
4.1.10 ”SYSTEM SETTINGS” AREA..................................................................................... 12
4.2 “DILUTER” FOLDER ............................................................................................................ 12
4.2.1 “PRIMING” AREA......................................................................................................... 12
4.2.2 “ASPIRATION AND DISPENSATION” AREA ........................................................... 13
4.2.3 “DILUTER ELECTROVALVE TEST” AREA .............................................................. 13
4.2.4 “MICROLITRES RESET” AREA .................................................................................. 13
4.2.5 “DRAWING” AREA ....................................................................................................... 13
4.3 “PHOTOMETER TEST” FOLDER ........................................................................................ 14
4.3.1 “AUTOZERO” AREA..................................................................................................... 14
4.3.2 “FILTER SELECTION AND START READING” AREA........................................... 15
4.3.3 “FILTERS READING” AREA....................................................................................... 15
4.3.4 “DRAWING” AREA ....................................................................................................... 16
4.4 “OPTICAL DENSITY” FOLDER ........................................................................................... 16
4.4.1 “ASPIRATION” AREA.................................................................................................. 16
4.4.2 “SELECT FILTER AND START READING” AREA.................................................. 17
4.4.3 “DRAWING” AREA ....................................................................................................... 17
4.5 “PERISTALTIC PUMP” FOLDER ....................................................................................... 18
4.5.1 “CALIBRATION” AREA ............................................................................................... 18
4.5.2 “ASPIRATION” AREA................................................................................................. 18
4.5.3 “ELECTROVALVE TEST” AREA ................................................................................ 19
4.5.4 “PERISTALTIC PUMP STEPS RESET” AREA............................................................ 19
4.6 “MISCELLANEOUS” FOLDER ........................................................................................... 19
4.6.1 “REACTION SECTORS” AREA.................................................................................... 20
4.6.2 “SETTINGS” AREA ....................................................................................................... 20
4.6.3 “SERIAL PORT” AREA ................................................................................................. 21
4.7 “PROGRAM DOWNLOAD” FOLDER ................................................................................ 21
4.8 “CUVETTE TEMPERATURE” FOLDER ............................................................................ 22
4.9 “PLATE TEMPERATURE” FOLDER................................................................................... 23

4 DIAGNOSTIC PROGRAM
The diagnostic program enables the operator to perform a complete check for each of the Vegasys
module functions.
This program has a folder structure, with each folder containing functions pertaining to the specific
module. To launch the program, the operator has to click with the left side of the mouse, on the
Diagnostic button located on the lower right side of the screen of the System Monitor (Fig. 1).
The Vegasys must be in the stand-by state to access this area.
“Diagnostic” button
Fig. 1 – System Monitor
Click on the “Diagnostic” button (Fig. 1), type the password “1234” in the “Password request”
dialog (Fig. 2) and click on its “OK” button to enter in the Diagnostic program.
Fig. 2 – “Password request” dialog
This operation can be canceled by clicking on “Exit” button of the “Password request” dialog (Fig. 2).

Once the “Diagnostic” program starts, the following two buttons are available:
: closes the screen and returns to the System Monitor.
: gives access to the Main Diagnostic Window.
Clicking on “Diagnostic” button the following screen appears (Fig. 3):
Fig. 3 – “Diagnostic” button
The following information for the options currently being used is given on this screen:
⇒ Icon Symbol for the Language Selected
⇒ Master Hardware Version
⇒ Serial Port
⇒ Firmware Version of the Analytical Control Board
⇒ Master Hardware DataBase Version
⇒ Serial Number of the Analytical Control Board
⇒ Master Hardware Ini Version
⇒ Instrument link

To enter in the “Sampling/Arm Plate Settings” folder, click on “Arm/Plate Setting” button (Fig. 4);
then, the “Input Operator Name and Password” dialog appears (Fig. 4):
Fig. 4 – “Input Operator Name and Password” dialog
If type the password “69231” in the “Password” field of the “Input Operator Name and Password”
dialog (Fig. 4), any name in its “Operator Name” field and give the confirm the “Sampling/Arm Plate
Settings” folder appears (Fig. 5):
Status table
Fig. 5 – “Sampling/Arm Plate Settings” folder

Otherwise, to enter in the “Sampling/Arm Plate Settings” folder and have the chance to enter in the
“Program Download”, “Cuvette Temperature” and “Plate Temperature” folders, click on “Arm/Plate
Setting” button (Fig. 4); then, the “Input Operator Name and Password” dialog appears (Fig. 4). If type
the password “28761” in its “Password” field, any name in its “Operator Name” field and give the
confirm the “Sampling/Arm Plate Settings” folder appears and “Download”, “Cuvette Temperature”,
“Plate Temperature” buttons appear (Fig. 6):
Fig. 6 – “Download”, “Cuvette Temperature”

and “Plate Temperature” buttons
The Diagnostic program has several buttons on the left side of the screen (Fig. 5 and Fig. 6). These
buttons refer to the following functions of the instrument:
Arm/Plate Setting: checks if the Sampling Arm, the Aspiration Arm and the Reactions Plate
are functioning correctly and performs mechanical calibration.
Diluter: checks the performance of the Diluter module.
Change Photometer Lamp: checks the performance of the Photometer Lamp.
Optical Density: measures the absorbance of the solution in the Micro-flow Cell.
Peristaltic Pump: to calibrate the aspiration of the Peristaltic Pump.
Miscellaneous: to reset the Reactions sectors, print the parameters set for the various modules
and to select a serial port from those identified by the program.

Download: to download and update the firmware of the Analytical Control Board.
Cuvette Temperature: to calibrate the temperature of the Micro-flow Cell.
Plate Temperature: to calibrate the temperature of the Reactions Plate.
The Status table (Fig. 5) shows - near every folder - the results (under the heading “Status”) of
various actions which have been run by the technician/operator in the Diagnostic program.
To save the new setted parameters exit from the pertinent folder, then select “Yes” in the
“MHVega” form.
4.1 “SAMPLING ARM/PLATE SETTINGS” FOLDER

The Fig. 7 shows the “Sampling/Arm Plate Settings” folder:
Status table
Fig. 7 – “Sampling/Arm Plate Settings” folder Settings table
The above folder is subdivided into the following sections:

⇒ General Reset
⇒ Washing Well
⇒ Reagents
⇒ Samples
⇒ Cuvette-Sampling

⇒ Cuvette-Aspiration
⇒ NO3
⇒ Free Cover
⇒ All Positions
⇒ System Settings
4.1.1 “GENERAL RESET” AREA

This area has the following commands:
: performs the resetting of the general system.
: vertically resets the Sampling and Aspirating arm.
: disengages the motors to enable the Sampling Arm and Reactions Plate
to be manually adjusted.
Note: to engage the motors again just select “General Reset”.
4.1.2 “WASHING WELL” AREA

The “Go – Washing Well” button (Fig. 7 and Fig. 8) brings the Sampling Arm to its home position
(Washing Well).
The relative “Down” button lowers the Sampling Probe of a number of steps specified in the small
window under the arrow (Fig. 7).
Modify the above mentioned number of steps by opportunely selecting the four small buttons placed
under the arrow; enable them (Fig. 8) by selecting the command “Go - Washing Well”.
Fig. 8 – “Washing Well” area
The four small buttons are the following:

: increases the number of steps of “1”;
: increases the number of steps of “5”;
: decreases the number of steps of “1”;
: decreases the number of steps of “5”.

Once the number of steps for the descent are defined, by selecting the command “Down” the
Sampling Probe goes down to the Washing Well using the number of steps programmed (in the Fig. 8,
60 steps).
4.1.3 “REAGENTS” AREA

The “Go – Reagent” button (Fig. 7 and Fig. 9) brings the Sampling Arm in the Reagents Plate
position #1 if the option “1” is selected, else in the Reagents Plate position #10 if the option “10” is
selected.
The relative “Down” button lowers the Sampling Probe down to the Reagent container of a number
of steps specified in the small window under its arrow (Fig. 9).
Modify the above mentioned number of steps down by opportunely selecting the four small buttons
placed under the arrow of the “Down” button; enable them (Fig. 9) by selecting the command “Go -
Reagent”.
Fig. 9 – “Reagents” area
The four small buttons for the “Down” command are the following:
: increases the number of steps down of “1”;
: increases the number of steps down of “5”;
: decreases the number of steps down of “1”;
: decreases the number of steps down of “5”.
The relative “Up” button rises up the Sampling Probe from the Reagent container of a number of
steps specified in the small window under its arrow (Fig. 9).
Modify the above mentioned number of steps up by opportunely selecting the four small buttons
placed under the arrow of the “Up” button; enable them (Fig. 9) by selecting the command “Go -
Reagent”.
The four small buttons for the “Up” command are the following:
: increases the number of steps up of “1”;
: increases the number of steps up of “5”;
: decreases the number of steps up of “1”;
: decreases the number of steps up of “5”.
It is possible to improve the centering of the Sampling Arm (Arm) with respect to the Reagents
Plate (Plate) position #1, if the option “1” is selected (else, with respect to the Reagents Plate position
#10 if the option “10” is selected), by selecting:
¾ the four small buttons placed above the “Go - Reagent” button to move the Sampling Arm
in a clockwise/counter-clockwise movement;
¾ the four small buttons placed under the “Go - Reagent” button to move the Plate in a
clockwise/counter-clockwise movement.

The four small buttons for the “Arm” are the following:
: moves the Sampling Arm clockwise 1 step at a time;
: moves the Sampling Arm clockwise 5 steps at a time;
: moves the Sampling Arm counter-clockwise 1 step at a time;
: moves the Sampling Arm counter-clockwise 5 steps at a time.
The same rules apply to the four small buttons of the “Plate” for moving the Reagents Plate with
respect to the Sampling Arm.
4.1.4 “SAMPLES” AREA

The “Go – Sample” button (Fig. 7 and Fig. 10) brings the Sampling Arm in the Samples Plate
position #1.
The relative “Down” button lowers the Sampling Probe down to the Sample cup of a number of
steps specified in the small window under its arrow (Fig. 10).
placed under the arrow of the “Down” button; enable them (Fig. 10) by selecting the command “Go -
Sample”.
Fig. 10 – “Samples” area
The same rules given in the “Reagents” area apply to the four small buttons of this “Down” button.
The relative “Up” button rises up the Sampling Probe from the Sample cup of a number of steps
specified in the small window under its arrow (Fig. 10).
Modify the above mentioned number of steps up by opportunely selecting the four small buttons
placed under the arrow of the “Up” button; enable them (Fig. 10) by selecting the command “Go –
Sample”.
The same rules given in the “Reagents” area apply to the four small buttons of this “Up” button.
It is possible to improve the centering of the Sampling Arm (Arm) with respect to the Samples Plate
(Plate) position #1 by selecting:
¾ the four small buttons placed above the “Go - Sample” button to move the Sampling Arm in
a clockwise/counter-clockwise movement;
¾ the four small buttons placed under the “Go - Sample” button to move the Plate in a
clockwise/counter-clockwise movement.
The same rules given in the “Reagents” area apply to the eight small buttons of the “Go - Sample”
button.

4.1.5 “CUVETTE-SAMPLING” AREA

The “Go / Cuvette-Sampling” button (Fig. 7 and Fig. 11) brings the first cuvette of the Reactions
Plate under the Sampling Arm.
The relative “Down” button lowers the Sampling Probe down to the cuvette of a number of steps
specified in the small window under its arrow (Fig. 11).
placed under the arrow of the “Down” button; enable them (Fig. 11) by selecting the command “Go /
Cuvette-Sampling” button .
Fig. 11 – “Cuvette-Sampling” area

The same rules given in the “Reagents” area apply to the four small buttons of this “Down” button.
It is possible to improve the centering of the Sampling Arm (Arm) with respect to the cuvette #1 of
the Reactions Plate (Plate) by selecting:
¾ the four small buttons placed above the “Go / Cuvette-Sampling” button to move the
Sampling Arm in a clockwise/counter-clockwise movement;
¾ the four small buttons placed under the “Go / Cuvette-Sampling” button to move the
Plate in a clockwise/counter-clockwise movement.
The same rules given in the “Reagents” area apply to the eight small buttons of the “Go / Cuvette-
Sampling” button.
4.1.6 “CUVETTE-ASPIRATION” AREA

The “Go / Cuvette-Aspiration” button (Fig. 7 and Fig. 12) brings the first cuvette of the Reactions
Plate under the Aspiration Arm if the option “1” is selected, else brings the cuvette #58 under it if the
option “58” is selected.
The relative first “Down” button lowers the Aspiration Probe down for the first descent into the
cuvette of a number of steps specified in the small window under its arrow (Fig. 12).
placed under the arrow of the first “Down” button; enable them (Fig. 12) by selecting the command
“Go / Cuvette-Aspiration” button.
The depth of the first descent is useful for aspirating liquid for the reaction (together with the
Peristaltic Pump) which is useful for emptying and rinsing the Micro-flow cell. This first descent
depends on the volume which remains in the cuvette of the Reactions Plate.
The relative second “Down” button lowers the Aspiration Probe down for the successive second
descent into the cuvette of a number of steps specified in the small window under its arrow (Fig. 12).

placed under the arrow of the second “Down” button; enable them (Fig. 12) by selecting the command
“Go / Cuvette-Aspiration” button.
This successive second descent should aspirate the minimum quantity of the reaction liquid
necessary for a reading.
First “Down” button Second “Down” button
Fig. 12 – “Cuvette-Aspiration” area
The same rules given in the “Reagents” area apply to the four small buttons of this two “Down”
buttons.
It is possible to improve the centering of the cuvette #1 of the Reactions Plate (Plate), if the option
“1” is selected (else, the centering of the cuvette #58 of the Reactions Plate if the option “58” is
selected), with respect to the Aspiration Arm (Arm) by selecting the four small buttons placed under
the “Go / Cuvette-Aspiration” button to move the Plate in a clockwise/counter-clockwise movement.
The same rules given in the “Reagents” area apply to the four small buttons of the “Go / Cuvette-
Aspiration” button.
4.1.7 “NO3” AREA

To be defined
4.1.8 “FREE COVER” AREA
: positions the Sampling Arm so that the instrument cover

can be remove.
4.1.9 “ALL POSITIONS” AREA
: allows to execute endlessly a specific cycle by moving the two Arms and
the Analytical Plate. Note: to stop, click on the “General Reset” button.

4.1.10 ”SYSTEM SETTINGS” AREA

The Settings table (Fig. 7) shows all the System Settings (under the heading “Value”).
4.2 “DILUTER” FOLDER

Open the “Diluter” folder by selecting the “Diluter” button (Fig. 13):
Status table
Fig. 13 – “Diluter” folder
This folder is subdivided into five sections:

⇒ Priming
⇒ Aspiration and Dispensation
⇒ Diluter Electrovalve Test
⇒ Microlitres Reset
⇒ Drawing
4.2.1 “PRIMING” AREA

This section has the command “PRIME - Priming”, the field “Cycles (1..9)” and the command
“STOP” (Fig. 13).
The command “PRIME - Priming” enables the operator to rinse the sampling hydraulic circuit.

The field “Cycles (1..9)” enables the operator to set the number of cycles to be run (Priming). The
minimum number of cycles to be run is 1 and the maximum is 9. To confirm the number of cycles
programmed, push “Enter” (from the keyboard).
The command “STOP” enables the operator to stop the “PRIME” procedure.
The operations performed during a “PRIME” cycle are the following:

¾ the Diluter aspirates 1000 µl of liquid from the loading bottle;
¾ the aspirated liquid is dispensed by the Sampling Probe into the Washing Well and
transferred by the Peristaltic Pump to the waste bottle.
4.2.2 “ASPIRATION AND DISPENSATION” AREA

This section has the command “Aspiration”, the field “µl” and the command “Dispensation
(HOME)” (Fig. 13).
The command “Aspiration” enables the operator to aspirate the number of microlitres previously
programmed in the field “µl”.
The field “µl” enables the operator to set the number of microlitres of liquid that the Sampling
Probe should aspirate. To confirm the number of microlitres programmed, push “Enter” (from the
keyboard).
The command “Dispensation (HOME)” enables the operator to dispense in the home position the
number of microlitres previously aspirated.
4.2.3 “DILUTER ELECTROVALVE TEST” AREA

This section has the command “EV Test” (Fig. 13).
This command allows to test the proper functioning of the Diluter Electrovalve. By selecting this
command the solenoid valve is automatically turned ON Æ OFF.
4.2.4 “MICROLITRES RESET” AREA

This section shows the number of micro-litres aspirated by the Diluter. It has the command
“Microlitres Reset” and the field “Total (µl)” (Fig. 13).
By selecting the command “Microlitres Reset” the count of the microlitres aspirated are zeroed and
visible in the field Total (µl)”.
4.2.5 “DRAWING” AREA
: shows the sampling hydraulic circuit of the instrument.

4.3 “PHOTOMETER TEST” FOLDER

Open the “Photometer Test” folder by selecting the “Change Photometer Lamp” button (Fig. 14):
Status table
Fig. 14 – “Photometer Test” folder
This folder is subdivided into four sections:

⇒ Autozero
⇒ Filter Selection and Start Reading
⇒ Filters Reading
⇒ Drawing
4.3.1 “AUTOZERO” AREA
: performs the automatic Autozero of the Photometer, where the instrument

will sample and aspirate distilled water twice (the liquid located in the
loading container). The values read are then reported on the table and
graph (in millivolt and counts) for each filter.
: reports and prints the last Autozero.
The command “STOP” enables the operator to stop the “AUTOZERO” procedure.

4.3.2 “FILTER SELECTION AND START READING” AREA

This section has the field “Select Filter” and the command “Start Reading” (Fig. 14).
The filed “Select Filter” allows the user to select a filter from a pull down menu list.
The command “Start Reading” allows to start the reading at the filter selected. It turns to “STOP
Reading” when selected to stop the reading (Fig. 15).
Æ Warning: make sure that the Micro-flow cell is filled of Rinse solution.
Fig. 15 – Start Reading
By selecting the “Start Reading” button the following appears on the screen (Fig. 15):
¾ a graph showing the time trend of the voltage read [“mv”] for the filter selected;
¾ a table which shows the voltage read [“mv”] for the filter selected.
4.3.3 “FILTERS READING” AREA
: this command automatically performs the reading of the liquid present in the
Micro-flow cell for each filter. It is important to make sure that the Micro-
flow cell is full of liquid.
The command “STOP” enables the operator to stop the “Filters Reading” procedure.

: shows the photometric system of the instrument.
4.4 “OPTICAL DENSITY” FOLDER

Open the “Optical Density” folder by selecting the “Optical Density” button (Fig. 16):
Status table
Fig. 16 – “Optical Density” folder
This folder is subdivided into three sections:

⇒ Aspiration
⇒ Select Filter and Start Reading
⇒ Drawing
4.4.1 “ASPIRATION” AREA

This section has the command “Aspiration” and the field “Aspiration Steps” (Fig. 16).
The command “Aspiration” allow to aspirate liquid from the Aspiration Probe (this is done by
removing the Aspiration Probe from the Aspiration Arm and dip it into the test solution).
The field “Aspiration Steps” enables the operator to program the number of steps of the Peristaltic
Pump in order to improve the aspiration of the liquid (Minimum number of steps = 1; Max = 4000). To
confirm the number of steps programmed, push “Enter” (from the keyboard).

4.4.2 “SELECT FILTER AND START READING” AREA

This section has the field “Select Filter” and the command “Start Reading” (Fig. 16).
The field “Select Filter” allows the user to select a filter from a pull down menu list.
The command “Start Reading” allows to start the reading at the filter selected. It turns to “STOP
Reading” when selected (Fig. 17), to stop the reading.
Æ Warning: make sure that the Micro-flow cell is filled of test solution.
¾ a graph showing the time trend of the O.D. read for the filter selected;
¾ a table which shows the O.D. and “mV” read for the filter selected.
: shows the photometric system of the instrument.

4.5 “PERISTALTIC PUMP” FOLDER

Open the “Peristaltic Pump” folder by selecting the “Peristaltic Pump” button (Fig. 18):
Status table
Fig. 18 – “Peristaltic Pump” folder
This folder is subdivided into four sections:

⇒ Calibration
⇒ Aspiration
⇒ Electrovalve Test
⇒ Peristaltic Pump Steps Reset
4.5.1 “CALIBRATION” AREA

This section (Fig. 18) allows to perform an automatic verification and adjustment of the Peristaltic
Pump aspiration.
: allows to carry out the calibration.
: these fields enables the operator to program the number of aspiration steps
for the Peristaltic Pump during calibration (“Final Aspirat.”) and the
number of calibration cycles to run (“Cycles (1..9)”). To confirm the two
numbers programmed, push “Enter” (from the keyboard).
The command “STOP” enables the operator to stop the calibration.
4.5.2 “ASPIRATION” AREA

This section (Fig. 18) has the command “Aspiration” and the field “Aspiration Steps”.
The command “Aspiration” allows to aspirate liquid away from the Aspiration Probe.

The field “Aspiration Steps” enables the operator to program the number of steps of the Peristaltic
Pump in order to improve the aspiration of the liquid (Minimum number of steps =1; Max= 4000). To
confirm the number of steps programmed, push “Enter” (from the keyboard).
4.5.3 “ELECTROVALVE TEST” AREA

This section has the command “EV Test” (Fig. 18).
This command allows to test the proper functioning of the Peristaltic Pump Electrovalve. By
selecting this command the solenoid valve is automatically turned ON Æ OFF.
4.5.4 “PERISTALTIC PUMP STEPS RESET” AREA

This section (Fig. 18) has the command “Pump Steps Reset”, the field “Total Steps” and the
command “Drawing”.
The command “Pump Steps Reset” enables the operator to cancel the total number of Peristaltic
Pump Steps.
The field “Total Steps” shows the total number of steps run by the Peristaltic Pump.
The command “Drawing” shows the aspiration hydraulic circuit of the instrument.
4.6 “MISCELLANEOUS” FOLDER

Open the “Miscellaneous” folder by selecting the “Miscellaneous” button (Fig. 19):
Status table
Fig. 19 – “Miscellaneous” folder
This folder is subdivided into three sections:

⇒ Reaction Sectors
⇒ Settings
⇒ Serial Port

4.6.1 “REACTION SECTORS” AREA

This section (Fig. 19) has the command “SECTORS – Reaction Sectors” and the field “First
Available Cuvette”.
The command “SECTORS – Reaction Sectors” allows to visualize the sectors and pertaining
reaction cuvettes used for analysis. See the following table:
Section heading
The reaction cuvettes used for the sample routine of the

instrument are indicated in red (in the example, 13
reaction cuvettes have been used in section SR1).
The reaction cuvette which have not been used are
indicated in green.
To zero a section, click with the mouse on the Section
heading. Once a section has been zeroed, the first
reaction cuvette in that section becomes automatically
available.
ÆImportant: it is possible to change the status of each single cuvette by clicking on the same.
The field “First Available Cuvette” indicates the first reaction cuvette available for sampling
(cuvette #54 in the Fig. 19).
S3/C16 - see Fig. 19 - indicates the sector and location available for sampling.
ÆImportant: the symbol “?” indicates that there are no reaction cuvettes available for the
sampling process.
4.6.2 “SETTINGS” AREA

This section (Fig. 19) has the command “Print Settings”, “Save Settings”, “Last Save Settings
Restore” and “First Save Settings Restore”.
The command “Print Settings” prints all system setting parameters (Step Sampling Probe Descent
to the Reagent, Step Sampling Probe Ascent to the Reagent, etc…).
The command “Save Settings” allows the technician/operator to save all new system setting
parameters.
The command “Last Save Settings Restore” allows the technician/operator to restore all last
system setting parameters.
The command “First Save Settings Restore” allows the technician/operator to restore all first
system setting parameters.

4.6.3 “SERIAL PORT” AREA
The command “Serial Port” allows the user to select a serial port
from a pull down menu list.
4.7 “PROGRAM DOWNLOAD” FOLDER

Open the “Program Download” folder by selecting the “Download” button (Fig. 20):
Status table
Settings table
Fig. 20 – “Program Download” folder
The command “FDT Download” (Fig. 20) allows the technician/operator to execute the download
procedure of the firmware in the instrument (using the FDT program).
The command “Self Download” (Fig. 20) allows the technician/operator to execute the download of
the firmware in the instrument when the firmware version is ≥ 1.14 or instrument link = USB.
The Settings table (Fig. 20) shows all the System Settings (under the heading “Value”).

4.8 “CUVETTE TEMPERATURE” FOLDER

Open the “Cuvette Temperature” folder by selecting the “Cuvette Temperature” button (Fig. 21):
Status table
Fig. 21 – “Cuvette Temperature” folder
The command “Start Reading” (Fig. 21) allows to start the temperature reading into the Micro-flow
cell. It turns to “STOP Reading” when selected (Fig. 22), to stop the reading.
¾ a graph showing the time trend of the cuvette temperature [°C];
¾ a table which shows the cuvette temperature [°C] and its value in counts.
The command “Auto - Regulation” (Fig. 21) allows to start the auto-regulation procedure of the
temperature into the Micro-flow cell. This regulation procedure stops automatically.

4.9 “PLATE TEMPERATURE” FOLDER

Open the “Plate Temperature” folder by selecting the “Plate Temperature” button (Fig. 23):
Status table
Fig. 23 – “Plate Temperature” folder

The command “Start Reading” (Fig. 23) allows to start the temperature reading of the Reactions
Plate. It turns to “STOP Reading” when selected (Fig. 24), to stop the reading.
¾ a graph showing the time trend of the plate temperature [°C];
¾ a table which shows the plate temperature [°C] and its value in counts.
It is possible to modify the Set Point of the plate temperature by selecting the four small buttons
placed under the “STOP Reading” button; enable them (Fig. 24) by selecting the command “Start
Reading” button.
The same above-mentioned rules apply to the four small buttons of the Set Point.
Æ Pay attention: the recommended Set Point is “1000” (as showed in Fig. 23).

Chapter 05 – SETTINGS AND ADJUSTMENTS
CHAPTER 05
- SETTINGS AND ADJUSTMENTS -
INDEX
5 SETTINGS AND ADJUSTMENTS……………………………….. 3
5.1 Sampling and Aspiration Arms……………………………………. 3

5.1.1 Replacement of the Sampling Arm…….…………………………….. 3
5.1.2 Replacement of the Aspiration Arm…….……………………………. 5
5.2 Diluter………………………………………………………………... 7
5.2.1 Replacement of the Diluter Assy……….……………………………. 7
5.2.2 Replacement of the Diluter Home Sensor……………………….…… 9
5.2.3 Replacement of the Diluter Electrovalve…………………………….. 10
5.2.4 Replacement of the Reel………………………………...…………… 10
5.3 Photometer…………………………………………………………... 12
5.3.1 Electronic adjustments of the Photometer……………………….…… 12
5.3.2 Replacement of the Photometer Assy………………………………... 14
5.3.3 Replacement of the Filters Drum Motor……………………………... 15
5.3.4 Replacement of the Filters Drum Home Sensor…………….……….. 16
5.3.5 Replacement of the Photometer Halogen Lamp……………………... 17
5.3.6 Replacement of the “A/D C Board” of the Photometer……………….. 19
5.3.7 Replacement of the Interferential Filters Kit…………………………. 20
5.3.8 Replacement of the Micro-flow Cell…………………………………. 21
5.4 Peristaltic Pump…………………………………………………….. 22

5.4.1 Adjustment of the Peristaltic Pump…….……………………….…… 22
5.4.2 Replacement of the Peristaltic Pump.………………………………... 24
5.4.3 Replacement of the Peristaltic Pump Motor…………………………. 26
5.4.4 Replacement of the Peristaltic Pump Electrovalve …………….……. 26
5.4.5 Replacement of the Peristaltic Pump Rotor…...……………………... 28

5.5 Central Mechanical Support……………………………………….. 29

5.5.1 Replacement of the Analytical Plate Stepper Motor…………………. 29
5.5.2 Replacement of the Sampling Arm Stepper Motor……..……………. 32
5.5.3 Replacement of the Analytical Plate Belt….………...………………. 34
5.5.4 Replacement of the Sampling Arm Belt……………………………... 35
5.5.5 Replacement of the Analytical Plate Home Sensor.…………………. 35
5.5.6 Replacement of the Sampling Arm Home Sensor.……..……………. 36
5.5.7 Replacement of the Plate Resistor……………………………………. 37
5.5.8 Replacement of the Plate Temperature Sensor………………………. 39
5.5.9 Replacement of the Thermostat……………………………………… 40

5 SETTINGS AND ADJUSTMENTS
5.1 SAMPLING AND ASPIRATION ARMS

5.1.1 Replacement of the Sampling Arm
1. Make sure that the VegaSys instrument is turned off.
2. Loosen the knurled knob of the Sampling Probe [Fig. 1 (a)] and remove it from its housing.
3. Unscrew the four fastening screws of the Sampling Arm cover (Fig. 1) and remove its two parts
from the arm.
4. Unplug carefully the motor connector from the Sampling Arm board.
5. Unscrew the Arm screw [Fig. 1 (b)] and remove the Sampling Arm from its axis.
Knurled knob
Cover
Fastening
screws
Arm screw
Sampling Probe
Fastening
Cover screw
(a) (b)
Fig. 1 [(a), (b)] – Sampling Arm
To replace the Sampling Arm (Fig. 3 – P/N: 1-15-0004-01) perform the following steps:
6. Replace the new Sampling Arm on its axis and screw slightly the Arm screw [Fig. 1 (b)].
7. Plug carefully the motor connector in the Sampling Arm board.
8. Replace the two parts of the cover on the arm inserting carefully the tube of the probe into its
small canal on the cover, then screw its four fastening screws (Fig. 1).
9. Replace the Sampling Probe in its housing and tighten its knurled knob [Fig. 1 (a)].
10. Rotate the Sampling Arm in the right toward in order to obtain the centering with respect to the
Washing Well (Fig. 3), then tighten the Arm screw [Fig. 1 (b)].
11. Turn on the VegaSys system (instrument and computer) and launch the “Diagnostic” program.
12. Select “Sampling Arm/Plate Settings” folder and request an “General Reset” (Fig. 2). Wait
until the reset instrument procedure has been completed.

Fig. 2 – “Sampling Arm/Plate Settings” folder of the Diagnostic program
After having mechanically centered the Sampling Probe over the Washing Well (compare the above
step 10), regulate all the other positions by using the buttons included in the “Sampling Arm/Plate
Settings” folder (Fig. 2) as described in the following instructions:
13. Select “Go Æ Washing Well” button. Make sure that the number placed below the relative
“Down” button is “60” (as showed in Fig. 2). If it not be so, insert the number “60” by using
the relative shift buttons.
14. Select the “Go Æ Reagent” button. The Sampling Probe will automatically position itself over
the Reagent in position # 1.
15. Select the relative “Down” button. If necessary, adjust the centering of the Sampling Probe
with respect to the reagent container opening by using the buttons placed above and below the
“Go Æ Reagent” button (“Arm - shift buttons” used to adjust the position of the Sampling
Arm with respect to the Analytical Plate and “Plate - shift buttons” used to adjust the position
of the Analytical Plate with respect to the Sampling Arm).
16. Adjust the descent of the Sampling Probe using the relative “Down” button. Make sure that the
distance between the bottom of the reagent container and the tip of the Sampling Probe is about
2 mm. If it not be so, adjust by using the relative shift buttons to change the number of steps.
17. Adjust the upward movement of the Sampling Probe by using the relative “Up” button. Make
sure that the tip of the Sampling Probe comes out of the cleaning pad by about 1 mm. If it not
be so, adjust by using the relative shift buttons to change the number of steps.
18. Next to the “Go Æ Reagent” button, select option “10”.
19. Click on “Go Æ Reagent” button. The Sampling Probe will automatically place itself over the
Reagent in position # 10.
20. Repeat the above steps in the following order: 15, 16 and 17 (for the position # 10 of the
Reagent).

21. Select the “Go Æ Sample” button. The Sampling Probe will automatically position itself over
the Sample in position # 1.
22. Select the relative “Down” button. If necessary, adjust the centering of the Sampling Probe
with respect to the sample container opening by using the buttons placed above and below the
“Go Æ Sample” button (“Arm - shift buttons” used to adjust the position of the Sampling
Arm with respect to the Analytical Plate and “Plate - shift buttons” used to adjust the position
of the Analytical Plate with respect to the Sampling Arm).
23. Adjust the descent of the Sampling Probe by using the relative “Down” button. Make sure that
the distance between the bottom of the sample container and the tip of the Sampling Probe is
about 2 mm. If it not be so, adjust by using the relative shift buttons to change the number of
steps.
24. Adjust the upward movement of the Sampling Probe by using the relative “Up” button. Make
sure that the tip of the Sampling Probe comes out of the cleaning pad by about 1 mm. If it not
be so, adjust by using the relative shift buttons to change the number of steps.
25. Select the “Go Æ Cuvette-Sampling” button. The Sampling Probe will automatically position
itself over the cuvette # 1.
26. If necessary, adjust the centering of the Sampling Probe with respect to the cuvette opening by
using the buttons placed above and below the “Go Æ Cuvette-Sampling” button (“Arm - shift
buttons” used to adjust the position of the Sampling Arm with respect to the Analytical Plate
and “Plate - shift buttons” used to adjust the position of the Analytical Plate with respect to the
Sampling Arm).
27. Make sure that the number placed below the relative “Down” button is “160” (as showed in
Fig. 2). If it not be so, insert the number “160” by using the relative shift buttons.
28. Select the “General Reset” button (Fig. 2). Wait until the reset instrument procedure has been
completed.
29. Exit from “Sampling Arm/Plate Settings” folder, then select “Yes” in the “MHVega” form to
save the new setting parameters.
If necessary, perform at least five successive cycles of the Priming procedure to

fill the hydraulic circuit with the liquid by selecting “PRIME – Priming” button in the
“Diluter” folder of the “Diagnostic” program.
5.1.2 Replacement of the Aspiration Arm
Considering that the Aspiration Arm is alike at the Sampling Arm, make reference
Note
to the Fig. 1 for its replacement procedure.

2. Loosen the knurled knob of the Aspiration Probe [Fig. 1 (a)] and remove it from its housing.
3. Unscrew the four fastening screws of the Aspiration Arm cover (Fig. 1) and remove its two
parts from the arm.
4. Unplug carefully the motor connector from the Aspiration Arm board.
5. Unscrew the Arm screw [Fig. 1 (b)] and remove the Aspiration Arm from its axis.

Instrument cover
Washing Well
Aspiration Arm
Photometer
cover
Sampling Arm
Analytical Plate
Chassis
Quarter-turn
locks
Fig. 3 – Instrument
To replace the Aspiration Arm (Fig. 3 – P/N: 1-15-0005-01) perform the following steps:
6. Replace the new Aspiration Arm on its axis and screw slightly the Arm screw [Fig. 1 (b)].
7. Plug carefully the motor connector in the Aspiration Arm board.
8. Replace the two parts of the cover on the arm inserting carefully the tube of the probe into its
small canal on the cover, then screw its four fastening screws (Fig. 1).
9. Center the Aspiration Arm with respect to the relative opening on the chassis, then tighten the
Arm screw [Fig. 1 (b)].
10. Replace the Sampling Probe in its housing and tighten its knurled knob [Fig. 1 (a)].
12. Select “Sampling Arm/Plate Settings” folder and request an “General Reset” (Fig. 2). Wait
until the reset instrument procedure has been completed.

After having mechanically centered the Aspiration Arm with respect to the relative opening on the
chassis (compare the above step 9), regulate the other positions by using the buttons included in the
“Sampling Arm/Plate Settings” folder (Fig. 2) as described in the following instructions:
13. Select “Go Æ Cuvette-Aspiration” button, then select the first “Down” button. If necessary,
adjust the centering of the Sampling Probe with respect to the cuvette opening by using the
buttons placed below the “Go Æ Cuvette-Aspiration” button (“Plate - shift buttons” used to
adjust the position of the Analytical Plate with respect to the Aspiration Arm).
14. Make sure that the number placed below the first “Down” button is “94” (as showed in Fig. 2).
If it not be so, insert the number “94” by using the relative shift buttons.
15. Adjust the second descent of the Sampling Probe by using the second relative “Down” button.
Make sure that the distance between the bottom of the cuvette and the tip of the Sampling
Probe is about 2 mm. If it not be so, adjust by using the relative shift buttons to change the
number of steps.
16. Next to the “Go Æ Cuvette-Aspiration” button, select option “58”.
17. Click on “Go Æ Cuvette-Aspiration” button. The Sampling Probe will automatically place
itself over the cuvette # 58.
18. Repeat the above steps in the following order: 13, 14 and 15 (for the cuvette # 58).
19. Select the “General Reset” button (Fig. 2). Wait until the reset instrument procedure has been
completed.
20. Exit from “Sampling Arm/Plate Settings” folder, then select “Yes” in the “MHVega” form to
save the new setting parameters.
5.2 DILUTER
5.2.1 Replacement of the Diluter Assy
2. Center the Sampling Arm with respect to the relative opening on the chassis, unscrew the four
quarter-turn locks (Fig. 3) and remove the chassis from the instrument.
3. Unscrew the two fittings from the head of the Diluter (Fig. 4), paying attention to the spillage of
liquid from the tubes.
4. Unscrew the four fastening screws of the cover of the Analytical Control Board and remove the
cover from its housing.
5. Unplug the J5 and J9 connectors from the Analytical Control Board (compare the scheme
SC-30-02314-00).
6. Unscrew the two fastening screws of the Diluter mechanical support (Fig. 4) and take out it
from the instrument.
7. Unscrew the four fastening screws of the Diluter (Fig. 4) and take out it from its mechanical
support.

Head of the Diluter

Fitting
Diluter fastening screw

Ref. 1 tube
Ref. 4 tube
Diluter mechanical support
Fastening screw
Fastening screw
of the Diluter
mechanical support
Reel
Diluter
Diluter Electrovalve
Diluter Electrovalve fastening screw
Fig. 4 – Diluter, Diluter Electrovalve and Reel
Fig. 5 – Diluter Assy [P/N: 10-00496-01]

To replace the new Diluter Assy (Fig. 5) perform the following steps:
8. Repeat the above steps in inverse order: from 7 to 3.
9. Turn on the VegaSys system (instrument and computer) and launch the Diagnostic program.
10. Select “PRIME - Priming” button in the “Diluter” folder. Wait until the priming procedure has
been completed (Note: perform at least five successive cycles of the priming procedure, if
necessary, to fill the hydraulic circuit with the liquid).
11. Select the “Exit” button to exit from the Diagnostic program.
12. Turn off the VegaSys instrument.
13. Replace the chassis on the instrument and screw its four quarter-turn locks (Fig. 3).
5.2.2 Replacement of the Diluter Home Sensor

3. Unscrew the four fastening screws of the cover of the Analytical Control Board and remove
the cover from its housing.
4. Unplug the J9 connector from the Analytical Control Board (compare the scheme
SC-30-02314-00).
5. Unscrew the two fastening screws of the Diluter Home Sensor (Fig. 6) and take out it from its
housing in the Diluter.
Head of the Diluter
Diluter Home Sensor
Diluter Home Sensor

fastening screws
Fig. 6 – Diluter Home Sensor Fig. 7 – Diluter Home Sensor

fastening screws [P/N: 9-10-0024-52]
To replace the new Diluter Home Sensor (Fig. 7) perform the following steps:
6. Replace the home sensor in its housing in the Diluter as showed in Fig. 6 and screw its two
fastening screws (Fig. 6).

5.2.3 Replacement of the Diluter Electrovalve

3. Unscrew the four fastening screws of the cover of the Analytical Control Board and remove
the cover from its housing.
SC-30-02314-00).
5. Take out the Ref. 1 and Ref. 4 tubes from the Diluter Electrovalve.
6. Unscrew the two fastening screws of the Diluter Electrovalve (Fig. 4) and take out it from the
Diluter mechanical support (Fig. 4).
To replace the new Diluter Electrovalve (Fig. 4 – P/N: 05-01539-50) perform the following steps:
7. Replace the electrovalve in its housing on the Diluter mechanical support as showed in Fig. 4
and screw its two fastening screws (Fig. 4).
10. Select “EV Test” button in the “Diluter” folder. Make sure that the Diluter Electrovalve is
correctly activated.
11. Select “PRIME - Priming” button in the “Diluter” folder. Wait until the priming procedure has
been completed (Note: perform at least five successive cycles of the priming procedure, if
5.2.4 Replacement of the Reel

3. Unscrew the fitting Reel (Fig. 8), paying attention to the spillage of liquid from the tube.
4. Take off the tube of the Reel (Fig. 8) from the Sampling Probe [Fig. 1 (a)], paying attention to
the spillage of liquid from the tube.
5. Unscrew the fastening screw of the Reel (Fig. 4 and Fig. 8) and take out it from the instrument.
To replace the new Reel (Fig. 8 – P/N: 041032/1) perform the following steps:
6. Replace the Reel in its position on the instrument as showed in Fig. 8 and screw its fastening
screw (Fig. 8).
7. Replace the tube of the Reel in correct mode as showed in Fig. 4 and Fig. 8.

Ref. 1 tube
Ref. 4 tube
Fitting Reel
Fastening screw
Diluter Electrovalve
Tube of the Reel Reel
Fig. 8 – Diluter Electrovalve and Reel
10. Select “PRIME - Priming” button in the “Diluter” folder. Wait until the priming procedure
has been completed (Note: perform at least five successive cycles of the priming procedure, if
11. Verify the absence of liquid leakage from the hydraulic circuit.
12. Verify the absence of air bubbles in the hydraulic circuit.

5.3 PHOTOMETER
5.3.1 Electronic adjustments of the Photometer
2. Unscrew the fastening screw of the Photometer cover (Fig. 9) and remove it from the
instrument.
3. Make sure that the voltage on the Lamp connector of the Photometer Lamp (Fig. 20) is equal to
6.0 Volts ± 0.1. If it not be so, turn the TR1 trimmer of the Analytical Control Board (compare
the scheme SC-30-02314-00) for adjusting the voltage.
4. Select “AUTOZERO” button in the “Photometer Test” folder (Fig. 10). Wait until the requested
operation has been completed.
Before applying the following electronic adjustments procedure, make sure

optically that the Micro-flow Cell (Fig. 11) is liquid-filled and without air bubbles.
Photometer cover
Fastening screw
Fig. 9 – Photometer cover
5. Make sure that the mV value of the Dark, visuliazed in the “mV (Last)” column of the reading
table, is within voltage range indicated in the “Range (mV)” column and about 2 mV
(recommended value).
Fig. 10 – “Photometer Test” folder of the Diagnostic program

If it not be so, performing the following steps:

a. select “Dark” in the “Select Filter” area (Fig. 10);
b. select the “Start Reading” button (Fig. 10);
c. read the Dark voltage value showed in the diagnostic folder and turn the OFFSET
trimmer of the “A/D C Board” (Fig. 12) in order to set the correct voltage value
(recommended value: 2 mV);
d. click the ”STOP Reading" button;
e. select the “Filters Reading” button (Fig. 10) and wait until the reading operation
has been completed;
f. repeat the above step 5.
Ref. 6 tube
Ref. 5 tube
Photometer protection
Offset trimmer Gain trimmer
Flat cable Micro-flow

connector Cell
Micro-flow Fastening screw

Cell
Fig. 11 – Photometer protection Fig. 12 – GAIN and OFFSET trimmers
of the “A/D C Board”
6. Make sure that the mV value of the filter which transmits the highest signal, visuliazed in the
“mV (Last)” column of the reading table, is within voltage range indicated in the “Range
(mV)” column and about 500 mV (recommended value). If it not be so, performing the
following steps:
a. select the filter which transmits the highest signal in the “Select Filter” area (Fig. 10);
b. select the “Start Reading” button (Fig. 10);
c. read its voltage value showed in the diagnostic folder and turn the GAIN trimmer of
the “A/D C Board” (Fig. 12) in order to set the correct voltage value (recommended
value: 500 mV);
d. click the ”STOP Reading" button;
e. select the “Filters Reading” button (Fig. 10) and wait until the reading operation
has been completed;
f. repeat the above step 6.

7. Make sure that the mV value of each other’s filters, visuliazed in the “mV (Last)” column of
the reading table, is within relative voltage range indicated in the “Range (mV)” column and
shorter than voltage value of the filter which transmits the highest signal.
9. Replace the Photometer cover on the instrument and screw its fastening screw (Fig. 9).
5.3.2 Replacement of the Photometer Assy

SC-30-02314-00), then unplug the flat cable connector from the A/D C Board connector.
5. Loosen the two screws of the Lamp connector (Fig. 20) and take off the two terminal wires
(Fig. 21) from the connector.
6. Take out the Micro-flow Cell (Fig. 13) from its housing (Fig. 22).
7. Unscrew the four fastening screws of the Photometer (Fig.13) and remove it from the
instrument.
To replace the Photometer Assy (Fig. 14) perform the following steps:
9. Perform the procedure “Electronic adjustments of the Photometer” described into the previous
Section 5.3.1.
Photometer
Micro-flow Cell
Fastening screw
Lamp wires
Fastening screw
Fig. 13 – Photometer Assy in the instrument

Photometer protection
Fastening screw
Fig. 14 – Photometer Assy
5.3.3 Replacement of the Filters Drum Motor

1. Remove the Photometer Assy (Fig. 14) from its housing performing the relative procedure
described into the previous Section 5.3.2, from step 1 to step 7.
2. Unscrew the fastening screw of the Photometer protection (Fig. 14) and remove it from the
Photometer.
3. Loosen the Pulley set screw (Fig. 15).
4. Unscrew the two fastening screws of the Filters Drum Motor (Fig. 16) and take out it from the
bottom side of the photometer plane (Fig. 17).
Filters Drum Filters Drum

Pulley set screw Motor pulley
Photometer
plane
Photometer
plane
1.5 mm
Filters Drum
Motor
Motor fastening screws
Fig. 15 – Filters Drum Motor pulley Fig. 16 – Drum Motor fastening screws

Filters Drum
Motor
Photometer plane
(bottom side)
Filters Drum
Home Sensor
Fig. 17 – Photometer Assy (bottom view)
To replace the new Filters Drum Motor (Fig. 17 – P/N: 05-02402-40) perform the following steps:
5. Insert the motor shaft in its housing of the Photometer plane as showed in Fig. 17, then screw
its two fastening screws as showed in Fig. 16.
6. Position the Filters Drum Motor pulley to 1.5 mm with respect to the upper side of the
Photometer plane (as showed in Fig.15), then tighten the Pulley set screw (Fig. 15).
7. Replace the Photometer protection on the Photometer and screw its fastening screw (Fig. 14).
8. Replace the Photometer Assy (Fig. 14) in its housing performing the relative procedure
described into the previous Section 5.3.2, from step 8 to step 9 (recommended step).
5.3.4 Replacement of the Filters Drum Home Sensor

Filters Drum
Channel of the Photometer
Fig. 18 – Filters Drum

Filters Drum
Fastening screw
Channel of the Photometer
Photometer plane
Filters Drum (upper side)
Home Sensor
Fig. 19 – Filters Drum Home Sensor
photometer.
SC-30-02314-00).
6. Position the Filters Drum as showed in Fig. 19.
7. Unscrew the fastening screw of the Filters Drum Home Sensor (Fig. 19) and take out it
from the upper side of the Photometer plane (Fig.19).
To replace the new Filters Drum Home Sensor (Fig. 19 – P/N: 9-10-0024-52) perform the
following steps:
8. Insert the home sensor in its housing as showed in Fig. 19, then screw its fastening screw
(Fig.19).
10. Perform the procedure “Electronic adjustments of the Photometer” described into the
previous Section 5.3.1 (recommended procedure).
5.3.5 Replacement of the Photometer Halogen Lamp

instrument.
3. Loosen the two screws of the Lamp connector (Fig. 20) and take off the two terminal wires
(Fig. 21) from the connector.

4. Unscrew the Lamp screw (Fig. 20) and take off the Photometer Lamp (Fig. 20) from its housing.
Photometer Lamp
Lamp connector
Lamp screw
Lamp wires
Fig. 20 – Photometer Lamp connector
To replace the new Photometer Halogen Lamp (Fig. 21 – P/N: 900154) perform the following
steps:
5. Insert the lamp in its housing as showed in Fig. 20, then screw the Lamp screw (Fig.20).
6. Repeat the above step 3 in inverse mode.
Section 5.3.1, from step 3 to step 9.
Lamp wires
Halogen Lamp
Terminal wires
Fig. 21 – Photometer Halogen Lamp

5.3.6 Replacement of the “A/D C Board” of the Photometer

instrument.
3. Take out the Micro-flow Cell (Fig. 13) from its housing (Fig. 22), then unplug the flat cable
connector from the A/D C Board connector.
4. Unscrew the two fastening screws of the A/D C Board cover (Fig. 22) and remove the cover
from the Micro-flow Cell case (Fig. 22).
5. Unscrew the fastening screw of the Micro-flow Cell case (Fig. 23) and remove it from the
Photometer.
6. Unscrew the two fastening screws of the A/D C Board (Fig. 24) and remove the board from
the Micro-flow Cell case (Fig. 24).
A/D C Board Micro-flow Cell

cover housing
A/D C Board
Case
fastening
screw
Board cover Micro-flow Cell

fastening screw case
Fig. 22 – Micro-flow Cell housing Fig. 23 – Micro-flow Cell case
Board
fastening screw
A/D C Board
Board
fastening screw
Micro-flow Cell case

A/D C Board connector
Fig. 24 – “A/D C Board” of the Photometer

To replace the new “A/D C Board” (Fig. 24 – P/N: 1-30-0001-00) perform the following steps:
7. Replace the board on the Micro-flow Cell case (as showed in Fig. 24) inserting the photodiode
in its hole of the case, then screw its two fastening screw (Fig. 24).
Do not touch light-sensitive portion of photodiode with your hands.
5.3.7 Replacement of the Interferential Filters Kit

2. Unscrew the fastening screw of the Photometer cover (Fig.9) and remove it from the instrument.
Photometer (Fig. 18).
4. For each filter position on the Filters Drum (Fig. 18 and Fig. 25) perform the following steps:
a. take out the retaining ring (Fig. 25) using the straight tweezers;
b. take out the interferential filter from its seating (Fig. 25).
Filter n°1 Filters Drum (outer side)

(position #2)
Dark
(position #1)
Filter n°2
(position #3)
Retaining ring
Fig. 25 – Interferential Filters

To replace the new Interferential Filters Kit (P/N: 65-02619-00), perform the following steps:
Do not touch the filters with your hands. Make use of tweezers to replace them.
5. For each filter position on the Filters Drum (Fig. 18 and Fig. 25) perform the following steps:
a. make sure that the filter wavelenght is right;
b. replace the filter in its seating, positioning the smooth side of the small filter box
on the outer side of the Filters Drum (Fig. 25);
c. insert (right to the end) the retaining ring in its seating on the small filter box
(as showed in Fig. 25), using the straight tweezers.
6. Replace the Photometer protection on the Photometer and screw its fastening screw (Fig. 11).
5.3.8 Replacement of the Micro-flow Cell

instrument.
3. Take out the Ref. 5 and Ref. 6 tubes from the Micro-flow Cell (Fig. 11).
4. Unplug the flat cable connector (Fig. 11) from the Micro-flow Cell connector (Fig. 26).
5. Take out the Micro-flow Cell (Fig. 26) from its housing (Fig. 22).
Micro-flow Cell
connector
Fig. 26 – Micro-flow Cell
To replace the new Micro-flow Cell (Fig. 26 – P/N: 041008), perform the following steps:
9. Select “Cuvette Temperature” folder, then click on “START Reading” button. Make sure that
the temperature in the Micro-flow Cell is 37,0 °C ± 0,2 °C [note: the cooling and heating cell are
respectively indicated by the lighting of the LD2 (green Led) and LD3 (red Led) of the
Analytical Control Board as showed in the scheme SC-30-02314-00].
10. Click on “STOP Reading” button, then select the “Exit” button to exit from the Diagnostic
program.

5.4 PERISTALTIC PUMP

5.4.1 Adjustment of the Peristaltic Pump
Before applying the following adjustment procedure, make sure that the Sampling and
Aspiration Arms are setted correctly (compare the above Sections 5.1.1 and 5.1.2).
instrument.
3. Put a drop of lubricating oil - with the P. P. sliding guide lowered - on the Ref. 8 tube, between
the P. P. Rotor and the P. P. sliding guide (Fig. 27).
Peristaltic Pump
Ref. 9 tube
Rotor Fitting
Ref. 8 tube
Peristaltic Pump
Electrovalve
Lock nut
Ref. 10 tube
Peristaltic Pump
Lock screw
sliding guide
Fig. 27 – Peristaltic Pump sliding guide
Peristaltic Pump
Rotor
Peristaltic Pump
Electrovalve
Peristaltic Pump
Lock screw
sliding guide
Fig. 28 – Peristaltic Pump and P.P. Electrovalve

4. Make sure that the stop plate nut is loosed and the stop plate (Fig. 30) is high-placed.
5. Draw up the Peristaltic Pump sliding guide to Ref. 8 tube (compare the Fig. 28), then screw its
lock screw (Fig. 28).
6. Insert a reagent bottle with Probe Rinse solution in the position #R1 of the Reagents Plate.
7. Make sure that a reaction rack is correctly inserted in the position #SR1 of the Analytical Plate.
8. Select “Aspiration” button in the “Peristaltic Pump” folder. Verify the free rotation of the
Peristaltic Pump Rotor during the execution of the requested aspiration procedure. If it not be
so, unscrew the lock screw (Fig. 28), draw up the Peristaltic Pump sliding guide to Ref. 8 tube
with less pressure, screw its lock screw, then repeat the step 8.
9. Insert the value “700” in the “Final Aspirat.” field, then select 5 cycles in the “Cycles (1..9)”
field of the “Peristaltic Pump” folder.
10. Select “Start Calibration” button in the “Peristaltic Pump” folder. Wait until the five calibration
procedures has been completed.
11. Make sure that the liquid (Probe Rinse solution) is situated - in every calibration procedure -
approximately between the point “A” of the Ref. 5 tube and the point “B” of the Ref. 6 tube – as
showed in Fig. 29 – in order to assure the Micro-flow Cell filling. If it not be so, perform the
following steps:
a. change the value in the “Final Aspirat.” field (note: increase the aspiration volume
increasing the value and vice versa);
b. repeat from the above step 10.
Liquid
A
Ref. 5 tube
Aspiration Arm
Ref. 6 tube
B Liquid
Liquid
Micro-flow Cell
Fig. 29 – Micro-flow Cell filling

12. Drag down the stop plate until you feel the stop (compare the Fig. 30), then screw the stop
plate nut (Fig. 30).
Peristaltic Pump
Motor
Stop plate nut
Peristaltic Pump Stop plate

mechanical support
Peristaltic Pump
Rotor
Fig. 30 – Stop plate of the Peristaltic Pump
The adjustment of the Peristaltic Pump is obtained by a right trade-off between

Note the pressure of the Peristaltic Pump sliding guide on the Ref. 8 tube and the value
introduced in the “Final Aspirat.” field.
5.4.2 Replacement of the Peristaltic Pump

SC-30-02314-00).
5. Unscrew the lock screw and drag down the Peristaltic Pump sliding guide (Fig. 27).
6. Take out the Ref. 8 tube from the fitting of the Ref. 9 tube (Fig. 27).
7. Take off the Ref. 8 tube from the Peristaltic Pump sliding guide.
8. Unscrew the lock nut of the Ref. 10 tube and take out latter from the P. P. Electrovalve (Fig. 27).
10. Unscrew the two fastening screws of the Peristaltic Pump mechanical support (Fig. 31) and
remove the Peristaltic Pump from the instrument.

Ref. 6 tube Peristaltic Pump

mechanical support
Lock nut
Peristaltic Pump Peristaltic Pump

Electrovalve Motor
Mechanical support
fastening screw
Fig. 31 – P. P. mechanical support fastening screws
To replace the Peristaltic Pump (Fig. 32), perform the following steps:
12. Perform the procedure “Adjustment of the Peristaltic Pump” described into the previous
Section 5.4.1.
Peristaltic Pump mechanical support
Peristaltic Pump
sliding guide
Peristaltic Pump Electrovalve
Fig. 32 – Peristaltic Pump mechanical support

5.4.3 Replacement of the Peristaltic Pump Motor
1. Remove the Peristaltic Pump from the instrument performing the relative procedure described
into the previous Section 5.4.2, from step 1 to step 10.
2. Make sure that the stop plate nut is loosed and the stop plate (Fig. 30) is high-placed.
3. Unscrew the two set screws of the P. P. Rotor (Fig. 35) and take out it from the shaft of the P. P.
Motor.
4. Unscrew the four screws of the Peristaltic Pump Motor (Fig. 33) and take out it from the
Peristaltic Pump mechanical support.
Peristaltic Pump
mechanical support
Peristaltic Pump Rotor
P. P. Motor screws
Peristaltic Pump sliding guide

Fig. 33 – Peristaltic Pump Motor screws
To replace the new Peristaltic Pump Motor (Fig. 31 – P/N: 05-02401-25), perform the following
steps:
6. Replace the Peristaltic Pump in the instrument performing the relative procedure described into
the previous Section 5.4.2, from step 11 to step 12.
5.4.4 Replacement of the Peristaltic Pump Electrovalve

4. Unplug the J21 connector from the Analytical Control Board (compare the sch. SC-30-02314-00).

8. Unscrew the two fastening screws of the Peristaltic Pump Electrovalve (Fig. 34) and take out it
from the instrument.
Peristaltic Pump Electrovalve
Ref. 8 tube
Fastening screws
Ref. 9 tube
Lock nut
Ref. 10 tube
Fig. 34 – Peristalitc Pump Electrovalve
To replace the new Peristaltic Pump Electrovalve (Fig. 34 – P/N: 05-01541-60), perform the
following steps:
11. Select “EV Test” button in the “Peristaltic Pump” folder. Make sure that the P. P. Electrovalve
is correctly activated.
12. Select “Start Calibration” button in the “Peristaltic Pump” folder. Verify visually that the
hydraulic circuit functioning pertinent to the Micro-flow Cell is correct.
13. Wait until all calibration procedures has been completed.
14. Select “PRIME - Priming” button in the “Diluter” folder. Verify visually that the hydraulic
circuit functioning pertinent to the Washing Well is correct.
15. Wait until the priming procedure has been completed.

5.4.5 Replacement of the Peristaltic Pump Rotor

3. Unscrew the two fastening screws of the Peristaltic Pump mechanical support (Fig. 31) and
twiddle it inside of the instrument.
4. Unscrew the two set screws of the Peristaltic Pump Rotor (Fig. 35) and take out it from the
shaft of the P. P. Motor (Fig. 35).
Peristaltic Pump mechanical support
Peristaltic Pump
P. P. Motor screw Motor
Rotor set screw
Peristaltic Pump Rotor
Peristaltic Pump sliding guide
Fig. 35 – Peristaltic Pump Rotor
To replace the Peristaltic Pump Rotor (Fig. 28 – P/N: 645006), perform the following steps:
6. Perform the procedure “Adjustment of the Peristaltic Pump” described into the previous
Section 5.4.1.
NOTE Replacement of the Peristaltic Pump Tube
Take-down the P. P. sliding guide (Fig. 27), take out the Peristaltic Pump Tube (Ref. 8 tube)
from the two fittings situated on its both terminal points, then take off it from the P. P. sliding
guide.
To replace the Peristaltic Pump Tube, repeat the above steps in inverse order and then
perform the procedure “Adjustment of the Peristaltic Pump” described into the previous
Section 5.4.1.

5.5 CENTRAL MECHANICAL SUPPORT

5.5.1 Replacement of the Analytical Plate Stepper Motor
3. Remove the six reaction racks and the Reagents Plate from the Analytical Plate (Fig. 37).
4. Unscrew the special threaded locking nut (Fig. 36) with the specific key, then remove the
Analytical Plate support from the Central Mechanical Support (Fig. 37).
Special threaded locking nut

Sampling Arm
Aspiration Arm
Analytical Plate
support
Support screw
Central Mechanical Support Support screws
Fig. 36 – Central Mechanical Support
7. Unscrew the three screws of the Central Mechanical Support (Fig. 36), then – carefully – skew it.
8. Unscrew the two fastening screws of the Analytical Plate Stepper Motor (Fig. 38 and Fig. 46).
9. Remove the Analytical Plate Belt from the pulley of the Analytical Plate Stepper Motor
(Fig. 39) and take out the motor from the Central Mechanical Support (Fig. 39).
10. Loosen the two set screws of the Analytical Plate Motor pulley and extract the pulley from the
motor shaft (Fig. 40).

Reagents Plate
Analytical Plate
support
Special threaded
locking nut
Central Mechanical
Support
Fig. 37 – Analytical Plate (exploded view)
Analytical Plate
axis
Sampling Arm
axis
Motor
connectors
Sampling Arm Analytical Plate

Stepper Motor Stepper Motor
Fig. 38 – Analytical Plate and Sampling Arm Stepper Motors

Central Mechanical
Support Analytical Plate
axis
Circular position
indicator
Analytical Plate
pulley Analytical Plate
Belt
Analytical Plate
Motor pulley Home Sensor
set screw Analytical Plate
Motor pulley
Fig. 39 – Analytical Plate Motor pulley
To replace the new Analytical Plate Stepper Motor (Fig. 40 – P/N: 05-02401-45) perform the
following steps:
11. Insert the pulley of the Analytical Plate Stepper Motor on the motor shaft (Fig. 40).
12. Position the pulley to 5.00 mm with respect to upper motor plane (Fig. 41), then tighten the two
set screws of the pulley (Fig. 41).
Set screw Analytical Plate Motor pulley
Upper motor plane
Motor shaft Set screw
Analytical Plate
Stepper Motor
Fig. 40 – Analytical Plate Stepper Motor Fig. 41 – Analytical Plate Motor pulley


14. Turn the Analytical Plate axis (Fig. 39) and make sure that the thoots of the belt are correctly
inserted on the Analytical Plate Motor pulley and on the Analytical Plate pulley (Fig. 39).
16. Perform the adjustments procedure of the Sampling Arm described into the previous
17. Perform the adjustments procedure of the Aspiration Arm described into the previous
5.5.2 Replacement of the Sampling Arm Stepper Motor

2. Loosen the knurled knob of the Sampling Probe [Fig. 1 (a)] and remove it from its housing.
3. Unscrew the four fastening screws of the Sampling Arm cover (Fig. 1) and remove its two parts
from the arm.
4. Unplug carefully the motor connector from the Sampling Arm board.
5. Unscrew the Arm screw [Fig. 1 (b)] and remove the Sampling Arm from its axis.
6. Unscrew the four quarter-turn locks of the chassis (Fig. 3) and remove it from the instrument.
Sampling Arm axis Sampling Arm pulley
Position indicator Pulley protection

sector
Sampling Arm Belt Motor pulley
Flywheel
Sampling Arm
Home Sensor
Sampling Arm Stepper Motor
Central Mechanical
Support Motor connector
Fig. 42 – Sampling Arm Stepper Motor

Flywheel
Set screw
Set screw
Central Mechanical
Support Sampling Arm Motor pulley
Sampling Arm Sampling Arm Motor

Stepper Motor fastening screw
Motor connector
Fig. 43 – Flywheel of the Sampling Arm Stepper Motor
Set screw
Sampling Arm Motor pulley
Upper motor plane
Motor shaft Set screw
Sampling Arm
Stepper Motor
Fig. 44 – Sampling Arm Stepper Motor Fig. 45 – Sampling Arm Motor pulley

9. Unscrew the two fastening screws of the pulley protection (Fig. 42) and remove it from the
Central Mechanical Support.
10. Loosen the two set screws of the flywheel of the Sampling Arm Stepper Motor (Fig. 43).
11. Unscrew the two fastening screws of the Sampling Arm Stepper Motor (Fig. 38 and Fig. 43).
12. Remove the Sampling Arm Belt from the pulley of the Sampling Arm Stepper Motor (Fig. 42).
13. Extract the flywheel from the pulley of the Sampling Arm Stepper Motor (Fig. 42).
14. Take out the Sampling Arm Stepper Motor from the Central Mechanical Support (Fig. 43).
15. Loosen the two set screws of the Sampling Arm Motor pulley and extract the pulley from the
motor shaft (Fig. 44).
To replace the new Sampling Arm Stepper Motor (Fig. 44 – P/N: 05-02402-40) perform the
following steps:
16. Insert the pulley of the Sampling Arm Stepper Motor on the motor shaft (Fig. 44).
17. Position the pulley to 6.00 mm with respect to upper motor plane (Fig. 45), then tighten the two
set screws of the pulley (Fig. 45).
19. Turn the Sampling Arm axis (Fig. 42) and make sure that the thoots of the belt are correctly
inserted on the Sampling Arm Motor pulley and on the Sampling Arm pulley (Fig. 42).
21. Replace the Sampling Arm on its axis and adjust it performing the previous Section 5.1.1, from
step 6 to step 29.
5.5.3 Replacement of the Analytical Plate Belt

1. Perform the replacement procedure of the Analytical Plate Stepper Motor described into the
previous Section 5.5.1, from step 1 to step 4.
3. Unscrew the two fastening screws of the Analytical Plate Stepper Motor (Fig. 38 and Fig. 46).
4. Remove the Analytical Plate Belt from the pulley of the Analytical Plate Stepper Motor
and from the Analytical Plate pulley (Fig. 39), then take out the belt from the Analytical Plate
axis (Fig. 39).
To replace the new Analytical Plate Belt (Fig. 39 – P/N: 1-35-0005-00) perform the following steps:
5. Wrap the new belt around the pulley of the Analytical Plate Stepper Motor and around the
Analytical Plate pulley (Fig. 39).
7. Perform the replacement procedure of the Analytical Plate Stepper Motor described into the

5.5.4 Replacement of the Sampling Arm Belt

1. Perform the replacement procedure of the Sampling Arm Stepper Motor described into the
2. Unscrew the two fastening screws of the pulley protection (Fig. 42) and remove it from the
Central Mechanical Support.
3. Loosen the two set screws of the flywheel of the Sampling Arm Stepper Motor (Fig. 43).
4. Unscrew the two fastening screws of the Sampling Arm Stepper Motor (Fig. 38 and Fig. 43).
5. Remove the Sampling Arm Belt from the pulley of the Sampling Arm Stepper Motor
and from the Sampling Arm pulley (Fig. 42), then take out the belt from the Sampling Arm axis
(Fig. 42).
To replace the new Sampling Arm Belt (Fig. 42 – P/N: 1-35-0005-00) perform the following steps:
6. Wrap the new belt around the pulley of the Sampling Arm Stepper Motor and around the
Sampling Arm pulley (Fig. 42).
8. Perform the replacement procedure of the Sampling Arm Stepper Motor described into the
5.5.5 Replacement of the Analytical Plate Home Sensor

Analytical Plate
Stepper Motor
Central Mechanical Support

(interior side)
Analytical Plate Motor

fastening screw
Analytical Plate Home Sensor
Fastening screw
Fastening screw
Fig. 46 - Analytical Plate Home Sensor

6. Unscrew the two fastening screws of the Analytical Plate Home Sensor (Fig. 46) placed on the
interior side of the Central Mechanical Support (Fig. 46), then take out the sensor from its
housing.
To replace the new Analytical Plate Home Sensor (Fig. 46 – P/N: 9-10-0024-52) perform the
following steps:
7. Position the new home sensor in its housing from interior side of the Central Mechanical
Support - as showed in Fig. 46 - in order to obtain that the circular position indicator is centered
with respect to the internal sides of the home sensor (Fig. 39).
8. Screw the two fastening screws of the Analytical Plate Home Sensor placed on the interior side
of the Central Mechanical Support (Fig. 46).
11. Select “Sampling Arm/Plate Settings” folder and request an “General Reset” (Fig. 2). Make
sure that the requested reset procedure is correctly performed.
12. Wait until the reset instrument procedure has been completed.
After applying the previous replacement procedure, make sure that the Sampling and
5.5.6 Replacement of the Sampling Arm Home Sensor

Fastening screw Sampling Arm Home Sensor
Fastening screw
Central Mechanical Support

(interior side)
Sampling Arm Stepper Motor
Fig. 47 – Sampling Arm Home Sensor

5. Unscrew the two fastening screws of the Sampling Arm Home Sensor (Fig. 42) placed on the
interior side of the Central Mechanical Support (Fig. 47), then take out the sensor from its
housing.
To replace the new Sampling Arm Home Sensor (Fig. 42 – P/N: 9-10-0024-52) perform the
following steps:
6. Position the new home sensor in its housing from interior side of the Central Mechanical
Support - as showed in Fig. 47 - in order to obtain that the position indicator sector is centered
with respect to the internal sides of the home sensor (Fig. 42).
7. Screw the two fastening screws of the Sampling Arm Home Sensor placed on the interior side
of the Central Mechanical Support (Fig. 47).
10. Select “Sampling Arm/Plate Settings” folder and request an “General Reset” (Fig. 2). Make
sure that the requested reset procedure is correctly performed.
11. Wait until the reset instrument procedure has been completed.
After applying the previous replacement procedure, make sure that the Sampling and
5.5.7 Replacement of the Plate Resistor

3. Remove the six reaction racks and the Reagents Plate from the Analytical Plate (Fig. 37).
4. Unscrew the special threaded locking nut (Fig. 36) with the specific key, then remove the
Analytical Plate support from the Central Mechanical Support (Fig. 37).
7. Unscrew the two fastening screws of the keep plate of the elastic band (Fig. 48), then remove the
keep plate.
8. Remove the elastic band from the Plate Resistor Support (Fig. 48).
10. Take out the connector of the Plate Resistor from its two wires.
11. Unplug the two faston connectors of the Thermostat from it (Fig. 51).
12. Remove the Plate Resistor from its support, slip off its wires from the through hole (Fig. 49),
then remove the Plate Resistor from the instrument.

Plate Resistor support
Elastic band Elastic band
Keep plate
Fastening screws

Fig. 48 – Elastic band
Thermostat
Plate Resistor
Through hole
Fig. 49 – Plate Resistor
To replace the new Plate Resistor (Fig. 49 – P/N: 31-02410-00) perform the following steps:
13. Repeat the above steps in inverse order: from 12 to 6, then from 4 to 3.
15. Make sure that the Plate Resistor is turned on.
16. Make sure that the LD1 – yellow Led Plate Resistor on the Analytical Control Board (compare
the sch. SC-30-02314-00) is lighted (heating plate).
17. Wait at least 30 minutes to reach thermal equilibrium of the instrument.

18. Make sure that the LD1 – yellow Led Plate Resistor on the Analytical Control Board (compare
the sch. SC-30-02314-00) blinks (holding plate temperature).
19. Select “Plate Temperature” folder, then click on “START Reading” button. Make sure of the
thermal equilibrium of the instrument.
20. Click on “STOP Reading” button, then select the “Exit” button to exit from the Diagnostic
program.
22. Replace the cover of the Analytical Control Board and screw its four fastening screws.
5.5.8 Replacement of the Plate Temperature Sensor

6. Unscrew the Plate Temperature Sensor from the Plate Resistor support (Fig. 50), then remove the
sensor from the instrument.
Plate Resistor
support
Plate Temperature Sensor
Fig. 50 – Plate Temperature Sensor
To replace the new Plate Temperature Sensor (Fig. 50 – P/N: 05-02400-40) perform the following
steps:
8. Perform the procedure described into the previous Section 5.5.7, from step 14 to step 23.

5.5.9 Replacement of the Thermostat

4. Unplug the two faston connectors of the Thermostat from it (Fig. 51).
5. Unscrew the two fastening screws of the Thermostat (Fig. 51), then remove it from the Plate
Resistor support.
Fastening screw
Faston Thermostat
Wire
Wire Faston
Plate Resistor
support Fastening screw
Fig. 51 – Thermostat
To replace the new Thermostat (Fig. 51 – P/N: 630077) perform the following steps:
8. Perform the procedure described into the previous Section 5.5.7, from step 14 to step 23.
Replacement of the:
Instrument Vain Fan Æ P/N: 05-01206-40
Board Vain Fan Æ P/N: 05-02403-30
Unscrew all fastening screws of the fan and unplug its connector from the Analytical Control
Board (compare the scheme SC-30-02314-00).
To replace the new fan, repeat the above steps in inverse order.

Chapter 06 - Maintenance
CHAPTER 06 - MAINTENANCE
INDEX
6. MAINTENANCE ............................................................................................................................ 2
6.1 PREVENTIVE MAINTENANCE ................................................................................................ 2
6.2 LIST OF PARTS SUBJECT TO WEAR AND USAGE .............................................................. 3
6.3 EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE....................... 3
6.4 WASH SOLUTION BOTTLE - CLEANING ............................................................................. 4
6.4.1 WASH SOLUTION BOTTLE CLEANING PROCEDURE ................................................ .....4
6.5 PROBE CLEANER PAD – REPLACING PROCEDURE.......................................................... 5
6.6 HYDRAULIC CIRCUIT WASHING.......................................................................................... 5
6.6.1 HYDRAULIC CIRCUIT CLEANING PROCEDURE ............................................................. 6
6.7 CHANGING THE PERISTALTIC PUMP TUBE....................................................................... 6
6.7.1 PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE .................................. 6
6.7.2 CALIBRATION PUMP PROCEDURE ................................................................................... 7
6.8 WASHING MICRO FLOW CELL - PROCEDURE................................................................... 8
6.9 CHANGING THE PHOTOMETER LAMP ................................................................................ 9
6.9.1 PROCEDURE FOR REPLACING THE PHOTOMETER LAMP ........................................... 9
6.10 CHANGING THE TUBES KIT................................................................................................ 11
6.10.1 PROCEDURE FoR REPLACING THE TUBES KIT ........................................................... 11
6.11 DECONTAMINATION PROCEDURE ................................................................................... 12
Vegasys Service Manual Rev.01 15 March 2009 Page 1

6. MAINTENANCE
This chapter describes the maintenance routine operations of the Vegasys. Maintenance activities,
should be carefully done in order to guarantee the proper working of the instrument over the time.
6.1 PREVENTIVE MAINTENANCE

The following schedule does not include the cleaning of reagent containers. Cleaning and decision
about eventual substitution of reagent containers must be made in conformity with good
laboratory practice.
MAINTENANCE SCHEDULE
FREQUENCY PROCEDURE NOTES WHO

DAILY – Before Check the levels of the
Operator
Start Work wash solutions.
Check the levels of
DAILY – Before
Reagents, Standards and Operator
Start Work
Controls.
Check the levels of the
DAILY – Before
Waste Bottle, if necessary, Operator
Start Work
empty it
Check the probe cleaner
DAILY – Before See “Probe Cleaner Pad –
pad and, if necessary, Operator
Start Work Replacing Procedure”
replace it
Clean the Sampling and See “External
DAILY – After Aspiration Probe using Sampling/Aspiration Probe Operator
Shutdown either paper toweling or Cleaning Procedure”
gauze
See “External
Clean the Sampling and
Sampling/Aspiration Probe
EVERY WEEK Aspiration Probe (with Operator
Cleaning Procedure” (see the
sodium hypoclorite)
Note of the procedure)
EVERY TWO Clean the wash solution See “Wash Solution Bottle
Operator
WEEKS bottle Cleaning Procedure”
See “Hydraulic Circuit
ONCE A MONTH Clean the Hydraulic Circuit Operator
Cleaning Procedure”.
EVERY SIX Change the Peristaltic See “Procedure For Replacing
Operator
MONTHS Pump Tube the Peristaltic Pump Tube”
Change all the tubes (Tubes See “Procedure For Replacing Service
ONCE A YEAR
Kit) The Tubes Kit” Engineer
EVERY 2000 Change the Photometer See “Procedure For Replacing
Operator
HOURS lamp The Photometer Lamp”
Note: the above-described maintenance schedule refers to a workload of approximately 500 tests
per day. The interval frequency may vary according to the instrument daily workload.

6.2 LIST OF PARTS SUBJECT TO WEAR AND USAGE
Description
Racks for Reaction Cells
Packs of Sample Cups of 0.8 ml
Reagent Containers
Plastic Bottles (H2O/Rinse & Waste)
Sampling Probe
Aspiration Probe
Tubing Kit Complete
Peristaltic Pump Tube
Probe Cleaner Pad
Photometer Lamp
Kit for E.V.Diluter connection
Diluter E.V.
Peristaltic Pump E.V.
Wash Solution
Diluter Head Teflon Fitting
Diluter Fitting
6.3 EXTERNAL SAMPLING/ASPIRATION PROBE CLEANING PROCEDURE
1) Turn off the Analyzer.

2) Loosen the knob which blocks the sampling/aspiration probe to the arm.
3) Remove the sampling/aspiration probe from the support frame.
4) Use only lint-free paper toweling or gauze.
5) Dampen the gauze or paper toweling with distilled water and clean outside of the
sampling/aspiration probe. Wipe the probe from the top downwards only! This is to avoid
that any bits of cloth, paper or lint fibers accidentally enter the probe itself.
6) Insert sampling/aspiration probe in the support frame of the arm.
7) Tighten the knob which blocks the sampling/aspiration probe in the arm.
Note: The manufacturer suggests that once weekly the above – described cleaning procedure be
performed using, instead of only distilled water, a 5% sodium hypochlorite solution to dampen the
gauze and then be repeated using distilled water.

6.4 WASH SOLUTION BOTTLE - CLEANING

During normal use and over time, mold and dust can build up inside the wash solution bottle.
For this reason, the bottle must be periodically washed.
How often the bottle must be cleaned depends on its use and on the quality of the distilled water
used in that particular laboratory. However, the manufacturer recommends thorough washing at
least once every two weeks.
It is important not to underestimate the risks associated with mold and dust particles. They are to
be regarded as a serious hazard as they can be the cause of instrument malfunction.
6.4.1 WASH SOLUTION BOTTLE CLEANING PROCEDURE
1) Turn off the Analyzer;

2) Pull the aspiration cannula out from the bottle;
3) Take the cap off the bottle and empty it;
4) Fill the bottle with 5 % sodium hypochlorite solution;
5) Clean the inside of the bottle using a brush in order to remove all traces of mold and/or
residue;
6) Leave the sodium hypochlorite solution stand in the bottle for at least ten minutes;
7) Empty the bottle, rinse it repeatedly and well with tap water, and then twice more using
distilled water;
8) Dry the bottle;
9) Fill the bottle with proper solution (Rinse);
10) Close the bottle;
11) Reconnect the aspiration cannula;
12) Carry out eight “PRIME” cycles checking that the sampling hydraulic circuit is filled of
liquid.

6.5 PROBE CLEANER PAD – REPLACING PROCEDURE
Located at the base of the sampling arm, the probe cleaner consists of a number (approximately
16) of circular paper strips mounted on a holder. These clean the sampling probe before and after
the aspiration and dispensing of reagents.
1) The Analyzer must not be in movement, if preferred Turn off it;

2) Remove the probe cleaner pad from its housing (located at the base of the sampling arm);
3) Replace the used probe cleaner pad with a new one.
6.6 HYDRAULIC CIRCUIT WASHING

During normal use and over time, mold and dust can build up inside the wash bottle and can have
a negative effect on the hydraulic circuit, compromising the correct functioning of the system.
For this reason, the hydraulic circuit must be periodically washed.
How often the hydraulic circuit must be washed depends on the operating conditions/environment
of the single instrument and the quality of the distilled water used in that particular laboratory. The
manufacturer recommends thorough washing at least once a month.
It is extremely important that the user not underestimate the risks associated with mold and dust
particles. They are to be regarded as a serious hazard as they can be the cause of instrument
malfunction.
The hydraulic circuit input cannula is located inside the wash solution bottle.

6.6.1 HYDRAULIC CIRCUIT CLEANING PROCEDURE
1) Turn on the Analyzer;

2) Prepare a bottle containing 500 ml of a 5% sodium hypochlorite solution;
3) Insert the aspiration cannula, located inside the wash solution bottle, into the bottle
containing the sodium hypochlorite solution;
4) Carry out eight “PRIME” cycles;
5) Carry out three ‘Autozero’ cycles;
6) Pull the aspiration cannula out from the bottle containing the sodium hypochlorite solution;
7) Clean and dry the aspiration cannula. Insert the aspiration cannula into a bottle containing
distilled water;
8) Carry out five “PRIME” cycles;
9) Carry out three ‘Autozero’ cycles;
10) Insert the aspiration cannula back into the wash solution bottle. Said bottle should have, in
the meantime, been cleaned and filled with a fresh supply of the required solution;
11) Carry out a “PRIME” cycle and then an “Autozero” cycle to check the system efficiency.
6.7 CHANGING THE PERISTALTIC PUMP TUBE

The replacement of the Peristaltic Pump tube is recommended every six months. Said frequency
may vary depending on the workload of the laboratory. The quality and reliability of this tube is
required to a correct emptying of the micro-flow cell.
6.7.1 PROCEDURE FOR REPLACING THE PERISTALTIC PUMP TUBE

The diameter and the length of the tubings for the flow system are very critical for the proper
functioning of Vegasys. Please make sure that the tubings are in accordance with the
manufacturer's specifications.
1) Switch off the instrument and unplug the power cord;
2) Loosen knob “A” and move block “B” downwards (see Figure at following page);
3) Disconnect the old tube from the junction and remove it from block “B”;
4) Replace the new tube and connect it again to the junction;
5) Push block “B” up to block “D” and tighten knob “A” (it is recommended to coat the
external surface of the tube with vaseline oil to lubricate the movement;
6) Check the aspiration volume using the "Start Calibration" function of the Vegasys
software, if necessary repeat the calibration;
7) Carry out an ‘Autozero’ cycle as functional check.

Tube Segment Tube Segment

I II
6.7.2 CALIBRATION PUMP PROCEDURE

This procedure enables the user to set up the volume of the reaction mixture that is aspirated by
the peristaltic pump from the reaction cell to the microflow cell via the aspiration tube.
Warning: before doing this procedure, one reagent container with Rinse Solution must be placed
on the analytical plate at reagent position R1.
1) Launch “Diagnostic” program, type the required Password to enter and wait for an
instrument general reset;
2) Press “Peristaltic Pump” button;
3) Press “Start Calibration” button; the instrument carries out a microflow cell wash cycle;
4) Make sure that the tubes leading to and from the microflow cell (see area circled) fill up of
liquid.
5) Referring to the tubes within the circled area in the figure:
- if the liquid goes too much beyond tube segment I, it means that there is too much liquid
- if the liquid does not completely fill the tube segment II, it is necessary to decrease the
steps in the “Final Aspiration” field. Push the “Start Calibration” button again and
check the liquid level in the tubes. On the other hand, if the liquid in segment II is too
much and in segment I there is not enough, increase the number of steps in the “Final
Aspiration” field. Push the “Start Calibration” button again and check the liquid level
in the tubes.
Note: If the adjustment cannot be made by adjusting the software, it is necessary to adjust the
pressure between the silicon tube and the peristaltic pump wheel through the keep plate of the
peristaltic pump.

6.8 WASHING MICRO FLOW CELL - PROCEDURE

This procedure enables the automatic washing of the microflow cell with a 5% sodium
hypochlorite solution. Before running this procedure, put some 5% sodium hypochlorite solution
into one of the reagent container.
1) Put some 5% sodium hypochlorite solution into one of the reagent container;
2) Place the reagent container with 5% sodium hypochlorite solution in position R1 of the
Reagent Plate;.
3) Ensure that the reaction rack SR1 is available in the analytical plate;
4) Launch the “Diagnostic” program, type the required Password and wait for the instrument
general reset;
5) Select “Peristaltic Pump” button;
6) Press “Start Calibration”; the sampling probe takes the sodium hypochlorite solution from
the reagent container and dispense it into the reaction cell N°19. The aspiration probe will
then take the solution in the reaction cell and the solution flows through the microflow cell;
7) Leave the sodium hypochlorite solution stand in the microflow cell for at least 15 minutes;
8) Remove the reagent container with sodium hypochlorite solution from “R1” position of the
Reagent Plate;
9) Place a reagent container with Distilled Water in position “R1” of the Reagent Plate;
10) Set the “N° Cycles” equal to 5;
11) Press “Start Calibration” then wait end of procedure.

6.9 CHANGING THE PHOTOMETER LAMP

The manufacturer suggests to replace the lamp after approximately 2000 hours of use. The lamp is
mounted on the photometer, which is located on the left-hand side of the reaction plate.
6.9.1 PROCEDURE FOR REPLACING THE PHOTOMETER LAMP
WARNING! DO NOT TOUCH THE GLASS PART OF THE LAMP WITH YOUR FINGERS! IF
NECESSARY, USE A CLEAN CLOTH TO REMOVE DUST, OR ALCOHOL TO REMOVE
MORE STUBBORN DIRT.

2) Unscrew the screw which holds the white cover panel of the photometer. Remove the white
cover panel.
3) Unthread the terminals of lamp. (designated as "A" in Figure).
4) Unscrew the block of lamp. (designated as "B" in Figure) and remove the lamp;
5) Replace the old lamp with a new one making sure that the pin is correctly inserted in the
alignment hole;
6) Make sure that the lamp regulator voltage is “6.0 Volt ± 0.1”; if not, turn the TR1 trimmer
located on the Vegasys Control Board.;
7) Remount the assembly by repeating the above steps in reverse order;
8) Turn the Analyzer on;
9) Launch “Diagnostic” program and type the required Password;
10) Select “Diagnostic” button and then, “Change Photometer Lamp”;
11) Make sure that the wash solution bottle is filled of proper and fresh solution;
12) Select “Autozero” button;

The Vegasys will automatically sample and aspirate wash solution twice, performing the autozero
and displaying a chart which states the read value expressed in “mV” and “Counts” for each filter.
Acceptable values (in “mV” and “counts”) are given below:
Filter Min. Value (mV) Max. Value (mV)

Dark 0.5 5
340, 405, 492, 510, 546, 578 e 620 280 512
If any filter is out of range, before adjust the Gain and Offset, check if the microflow cell is:
perfectly clean
full of liquid and that there are no air bubbles on the inside.
Then follow procedure “Electronic Adjustment of the Photometer” Chapter 05 Par.5.3.1 of this
manual.

6.10 CHANGING THE TUBES KIT

Over time and through normal use, the tubes become worn. The manufacturer suggests to replace
the tubes at least once a year.
6.10.1 PROCEDURE FOR REPLACING THE TUBES KIT

2) Unlock the four screws of the instrument case (make a quarter of turn for each screw);
3) Line up the sampling probe arm to the center of the Reaction Plate;
4) Remove the instrument case;
5) Replace the tubes following the indication displayed on the “Hydraulic Tubing Diagram”;
6) Turn on the Analyzer;
7) Launch the Vegasys software;
8) Launch “Diagnostic”program and type the required Password;
9) Click on “Diluter” button;
10) Carry out eight “PRIME” cycles to make sure that the sampling hydraulic circuit is filled
with liquid;
11) Click on “Change Photometer Lamp” button;
12) Click on “Autozero” button to make sure that the aspiration hydraulic circuit is filled with
liquid;
13) Line up the sampling arm to the center of the Reaction Plate;
14) Remount the instrument case and lock the four screws (make a quarter of turn for each
screw);
15) Carry out three “PRIME” cycles to make sure the sampling hydraulic circuit is filled of
liquid;
16) Carry out two “Autozero” cycles to check the system efficiency. If needed, carry out the
“Calibration Pump Procedure”.

6.11 DECONTAMINATION PROCEDURE

Before replacing any instrument parts, repairing any defective items or performing any instrument
maintenance procedure, the operator or maintenance technician must carry out the below-
described decontamination procedure for the instrument parts involved in the operations.
This procedure can be performed on:
the entire Analyzer
those parts of the instrument subject to possible contamination
NECESSARY MATERIAL
an ESOFENOL solution diluted to 6% (60 cc in one liter of distilled water). ESOFENOL is an
antibacterial and antiviral substance.
Rubber gloves
Mask
Lab coat
EXTERNAL SURFACES AND INDIVIDUAL PARTS

Spray the solution all over the instrument, paying particular attention to wetting:
- the sampling arm
- the reaction plate (including the cuvettes)
- the racks
- the instrument case
Allow the solution to stand for approximately 30 minutes
Wipe the solution off the instrument and the various components using a sponge dampened
with distilled water
Carry out a decontamination of the internal hydraulic circuit.
HYDRAULIC CIRCUIT (ENTIRE INSTRUMENT)

Fill a container with a 5% sodium hypochlorite solution;
Insert the aspiration cannula, located into the wash solution bottle, into a bottle filled with the
5% sodium hypochlorite solution and carry out 3 “Autozero” cycles;
Remove the aspiration cannula from the 5% sodium hypochlorite solution and immerge it in
another bottle containing Rinse Solution or Distilled Water;
Carry out 3 “Autozero” cycles;
Insert the aspiration cannula into its proper bottle.

Chapter 07 – HOST COMMUNICATION
CHAPTER 07
– HOST COMMUNICATION –
INDEX
7 HOST COMMUNICATION...........................................................................................................2
7.1 COMMUNICATION WITH THE HOST COMPUTER.............................................................2
7.1.1 COMMUNICATION PARAMETERS...........................................................................2
7.2 PROTOCOL SPECIFICATIONS ................................................................................................2
7.3 HEADER RECORD (H) ..............................................................................................................4
7.4 PATIENT RECORD (P) ..............................................................................................................6
7.5 TEST ORDER RECORD (O) ......................................................................................................9
7.6 RESULTS RECORD (R) ...........................................................................................................12
7.7 MESSAGE TERMINATOR RECORD (L)...............................................................................14
7.8 REQUEST INFORMATION RECORD (Q) .............................................................................15
7.9 COMMENT RECORD (C)........................................................................................................16
VegaSys Service Manual Rev. 01 15 March 2009 Page 1

7 HOST COMMUNICATION
7.1 COMMUNICATION WITH THE HOST COMPUTER

The Vegasys can be connected to a host computer for the purpose of facilitating results print-out
and patients management.
In order to enable communication between the Vegasys and the Host computer, select the “Host
Link” field under “Options” in the “Parameters” menu.
7.1.1 COMMUNICATION PARAMETERS

The Vegasys System is linked to the managing computer using an RS-232C serial connector
having the following specifications:
¾ Baud Rate: 110, 300, 600, 1200, 2400, 9600, 14400, 19200, 28800, 38400, 56000, 128000,
256000 Bit/sec.
¾ Data bits: 4, 5, 6, 7, 8
¾ Parity: E (even), O (odd), N (none), M (mark), S (space)
¾Stop bit: 1, 1.5, 2
Default communication parameters for host communication are:

¾ Baud Rate: 9600 Bit/sec.
¾ Data bits: 8
¾ Parity: N
¾Stop bit: 1
7.2 PROTOCOL SPECIFICATIONS

This part of Help (Protocol Specifications) contains information for the laboratory computer
and analyzer. This exchange of data follows specific ASTM protocols:
E 1381-95 Standard Specification for Low-Level Protocol to Transfer Messages

between Clinical Laboratory Instruments and Computer Systems;
E 1394-97 Standard Specification for Transferring Information between Clinical

Instruments and Computer Systems.
ÆASTM uses a number of different terms to indicate the way it groups data.
• Field: an individual piece of data often referred to as a data field or a data element.
• Record: a number of logically related data fields grouped together to form one part of a
complete message.
• Repeat field: a data field of the same type as the one immediately preceding it. A delimiter
separates one instance of a repeat field from the next.
• Component field: part of data field that might contain more than one piece of data.

The default communication configuration for the program is the following: "9600, N, 8, 1".
Æ ASTM uses record types that are common and familiar to all laboratory personnel. It uses the
following record types:
• Header Record (H): contains identifying information about the sending station,
conventions that the device uses for field recognition, and the date
and time of send station transmission.
• Patient Record (P): contains patient information and identification number.
• Test Order Record (O): contains information about the assay or requests themselves and
includes other data.
• Result Record (R): contains information about the outcome of individual tests for an
individual patient and follows a sample program record. The results
contain the actual measurements derived from the test and a
comparison of the individual result to certain ranges specified as
norms for the laboratory.
• Message Terminator Record (L): This is the last record in the message. A header record
may be transmitted after this record signifying the
start of a second message.
• Request Information Record (Q): is used by either clinical instruments or computer
systems for a remote request for information from its
reciprocal system.
• Comment Record (C): Comment records shall apply to any other record except the
message trailer record. They may be free standing messages sent
from the instrument, unrelated to a particular patient or test
procedure.
The instrument does not send or accept comment records.
The following scheme shows the “Download” and “Upload” operations between the Vegasys
system and the Host computer:
Vegasys system Host computer

Download
Upload

7.3 HEADER RECORD (H)

Down Up Max
Field Field Title Description and Valid Values
Load Load Len
Record Type This is a required field that contains an

1 R A 1
ID “H” identifying it as a header record.
The Analyzer System uses only the four

default values shown here. Delimiters
may not be duplicated. The field
delimiter follows the escape character to
separate the delimiter specification from
a subsequent field in the header record.
Using default values, the first six
characters of the header record will
2 Delimiters I A 4 appear using the following characters:
H I\^&I
Field Delimiter I
Repeat Delimiter \
Component Delimiter ^
Escape Delimiter &
Message
3 I N
Control ID
Access
4 I N
Password
Sender Name ‘SHAnalyzer’: this is the name of the
5 I A 10
or ID device that is sending the data.
Sender Street
6 I N
Address
7 Reserved Field I N
Sender Tel.
8 I N
Number
Characteristics
9 I N
of Sender
10 Receiver ID I N
Comments or
11 Special I N
Instructions
12 Processing I N
ASTM Version
13 I N
No.
Date and Time of transmission:
14 Date and Time I A 14
formatted as YYYYMMDDHHMMSS.

For example: 3:35 PM on March 1, 1995

would be represented using the
following characters:
19950301153500.
R Required D Down Load

O Optional U Up Load
Legend:
I Ignored N Never
A Always S Sometimes
Example Header Record Layouts (H)

Record sent by Host computer H | \ ^ | | | HOST | | | | | | | | |19950301153500<CR>
Record sent by Vegasys system H | \ ^ & | | | SHAnalyzer | | | | | | | | | 19950301154000<CR>

7.4 PATIENT RECORD (P)
Down Up Max
Load Load Len
This is a required field that contains a

1 Record Type ID R A 1
“P” identifying it as a patient record.
This field starts with a “1” for the
Sequence patient and is incremented by 1 for each
2 R A 3
Number additional patient within the
transmission.
Practice This field can be assigned by the
3 Assigned R A 15 instrument with no corresponding
Patient ID download.
Laboratory
4 Assigned I N
Patient ID
5 Patient ID No. 3 I N
This field has two components:
6 Patient Name O S 36 • Last Name (up to 20 characters)
• First Name (up to 15 characters).
Mother's
7 I N
Maiden Name
Formatted as YYYYMMDD: For
example, a birth date of December 1,
8 Birth Date O S 8
1980 would be represented as:
19801201
This field indicates the sex of the
patient. The possible values are:
• M: male
• F: female
• U: unspecified
¾ Download
If present and equal to “U”, the
patient will be considered Male or
Child depending to his birthdate
9 Patient Sex R O 1 received and to the Child/Adult Age
Limit set in the Vegasys system.
¾ Upload
If patient has been received from the
Host computer this field will be set
NULL. If the patient has not been
received from Host computer this
field will be:
o M: male
o F: female
o U: if the patient is Child

Patient
The Analyzer System will ignore this
10 Race/Ethnic I N
field at launch.
Origin
For Analyzer, this is a four- component
field:
• Address (25 characters)
11 Patient Address O S 60
• City (25 characters)
• State (2 characters e.g.: NY, IT)
• Zip (5 characters)
Patient Tel
13 I N
Number
Attending
14 I N
Physician ID
15 Special Field 1 I N
16 Special Field 2 I N
17 Patient Height I N
18 Patient Weight I N
Patient Known
19 or Suspected I N
Diagnosis
Patient Active
20 I N
Medications
21 Patient's Diet I N
Practice Field
22 I N
No. 1
Practice Field
23 I N
No. 2
Admission Date
Admission date only.
24 and Discharge O S 8
Formatted as YYYYMMDD.
Date (if desired)
Admission
25 I N
Status
26 Location O S 20
Nature of
Alternative
27 Diagnostic I N
Code and
classifiers
Alternative
28 Diagnostic I N
Code and

classification
29 Patient Religion I N
30 Marital Status I N
31 Isolation Status I N
32 Language I N
Hospital
33 I N
Service
Hospital
34 I N
Institution
Dosage
35 I N
Category

Legend:
I Ignored N Never
Example Patient Record (P)

Record sent by P | 1 | B108K | | | MW5910^Smith | | 19861002 | M | | Park Avenue^New
Host computer York^NY^10002 | | | | | | | | | | | | | 20020923 | | Hematology | | | | | | | | | |
Record sent by P | 1 | B108K | | | MW5910^Smith | | 19861002 | M | | Park Avenue^New
Vegasys system York^NY^10002 | | | | | | | | | | | | | 20020923 | | Hematology | | | | | | | | |

7.5 TEST ORDER RECORD (O)
Down Up Max
Load Load Length
Record Type This is required field that contains

1 R A 1
ID an “O” identifying it as an order
This field starts with “1” for the first

Test Order Record and is
incremented by 1 for each additional
Sequence Test Order Record within the
2 R A 3
Number record.
This will be reset to “1” whenever
another patient record is transmitted.
Although the operator can manually

edit this field at any time, the value
of this field is usually assigned by
3 Specimen ID R A 15 the laboratory computer before
down loading. The Analyzer uses
and reports its results based on the
assigned specimen ID.
Instrument
4 I N
Specimen ID
This is a four-component field:
• Universal Test ID Code
(not used)
I N
• Universal Test ID Name
I N (not used)
Universal Test • Universal Test ID Type
5 9
ID N (not used)
I
• Manufacturer's or local code
R A (6 characters):
this is the code defined in the
Analyzer.
This field indicates the test priority
codes.
¾ Download
If present and equal to “S” the
test will be considered as STAT.
If absent or present but different
6 Priority O A
from “S”, the test will be
considered with no priority.
¾ Upload
It will be set to “S” if the test is a
STAT, otherwise it will be a
NULL field.

Request
7 Ordered I N
Date/Time
Specimen
8 Collected I N
Date/Time
Collection End
9 I N
Time
Collection
10 I N
Volume/Units
11 Collector ID I N
This field indicates the action to be

taken with respect to the specimens
that accompany or precede the
request.
¾ Download
12 Action Code O N If present and equal to “C”, the
test will be cancelled.
If absent or present and not equal
to “C”, the test will be added.
¾ Upload
It will always be set to NULL.
13 Danger Code I N
Relevant
14 I N
Clinical Info.
Date/Time
15 Specimen I N
Received
This is a numeric field indicating the
type of specimen:
Specimen The Imm. System uses the following
16 R A 1
Type ASCII characters:
0= Serum
1= Urine
Ordering
17 I N
Physician
Physician Tel.
18 I N
Number
User Field
19 I N
No. 1

User Field
20 I N
No. 2
Lab Field
21 I N
No. 1
Lab Field
22 I N
No. 2
Date /Time
Result
23 I N
Reported Last
or Modified
Instrument
24 I N
Charge
Instrument
25 I N
Section ID
The field indicates the direction of
26 Record Type 1 the transmission:
I A
O - Down Loading
F - Up Loading
Location or
Ward of
28 I N
Specimen
Collection
Nosocomial
29 I N
Infection Flag
Specimen
30 I N
Service
Specimen
31 I N
Institution
Legend:
I Ignored N Never
Example Test Order Record Layouts (O)

Record sent by Host computer O | 1 | AR102 | | ^^^GLU | | | | | | | | | | | 0 | | | | | | | | | | O | | | | |
Record sent by Vegasys system O | 1 | AR102 | | ^^^GLU | | | | | | | | | | | 0 | | | | | | | | | | F | | | | |

7.6 RESULTS RECORD (R)

Down Up Max
Load Load Len
This is a required field that contains an

1 Record Type ID A 1
“R” identifying it as a Results Record.
This field starts with “1” for the first
result and is incremented by 1 for each
additional result within the record.
Sequence
2 A 3 This will be reset to “1” when the
Number
results from another test order record
are transmitted to the laboratory
computer.
This is a four-component field:
I N
• Universal Test ID Code
N (not used)
I
• Universal Test ID Name
N 9 (not used)
3 Universal Test ID I
• Universal Test ID Type
A (not used)
R
• Local Manufacturer's or local
code (6 characters): this is the
code defined in the Analyzer.
This field reports the test result and
could be set as:
• NULL if test is pending (see
field 9)
• “*” if extended flags require this
value
Data or
• “<=0” if result is minor or equal
4 Measurement A 10
to zero
value
• Maximum 10-character, floating
point field that includes the
decimal point. The number of
precision point digits will vary
according to the test and is
configurable on the Analyzer.
This is a field for up to 6 characters
5 Units of Measure A 6 that the operator defines for analytic
measurement.
This field has two components; one
giving the lower limit and the other the
6 Reference Ranges A 21 upper limit of the range. The format
for this field is N^N. Character “.” Is
the decimal separator used.
This field indicates the normal status
Result Abnormal
7 A 2 of the result. The following codes are
Flags
valid values:

L - Below Low normal

H - Above High normal
LL - Below Panic normal
HH - Above Panic normal
< - Below absolute low (under linearity)
> - Above absolute high (over linearity)
E – Edited
Nature of
8 Abnormality N
Testing
The Imm. System currently
implements only three valid values:
9 Result Status A 1 I - pending results in the instrument;

F - final results;
V - operator verified/approved result.
Date of Change in
Instrument
10 N
Normative Values
or Units
11 Operator ID N
Date/Time Test
12 N
Started
Date and Time of test completion:

Date/Time Test
13 A 14 formatted as
Completed
YYYYMMDDHHMMSS.
14 Instrument ID N

Legend:
I Ignored N Never
Example Result Record Layouts (R)

Record sent by
R | 1 | ^^^GLU | 70.97 | UL | 70^105 | N | | F | | | | 20020923114302 |
Vegasys system

7.7 MESSAGE TERMINATOR RECORD (L)

Down Up Max
Load Load Len
This is a required field that contains
Record Type
1 R A 1 an “L” identifying it as an Message
ID
Terminator Record.
Sequence For a message terminator, this
2 R A 1
Number message should always be “1”.
This indicates the cause of
Termination termination. The following codes are
3 R A 1 valid values for the Analyzer :
Code
Null or N-normal termination
Legend:
I Ignored N Never
Example Message Terminator Record Layout (L)

Record sent by Host computer L|1|N
Record sent by Vegasys system L|1|N

7.8 REQUEST INFORMATION RECORD (Q)

Down Up Max
Load Load Len
This is a required field that
1 Record Type ID A 1 contains a “Q” identifying it as a
request.
2 Sequence Number A 1 It is always “1”.
This field can either be:

"ALL" - to mean all
demographics and tests being
ordered should be sent to the
Starting Range ID instrument at this time,
3 A 31
Number or can have two components:
• Computer system patient ID
No. (up to 15 characters);
• Computer system specimen
ID No. (up to 15 characters).
Ending Range ID
4 N
Number
5 Universal Test ID N
Nature of Request
6 N
Time Limits
Beginning Request
7 Results Date and N
Time
Ending Request
8 Results Date and N
Time
Requesting
9 N
Physician Name
Requesting
10 Physician N
Telephone Number
11 User Field No. 1 N
12 User Field No. 2 N
Request Information It is always "O" (requesting test
13 Status Codes
A 1
orders and demographics only).
Example Request Information Record Layouts (Q)

H | \^& | | | SHAnalyzer | | | | | | | | | 20020927100402
Record sent by Vegasys system Q | 1 | ALL | | | | | | | | | | O
L|1|N

7.9 COMMENT RECORD (C)
Down Up Max
Load Load Len
This is a required field that contains a

1 Record Type ID I A 1
“C” identifying it as a comment record.
This field starts with a “1” for the
comment record and is incremented by
Sequence
2 I A 3 1 for each additional comment with
Number
reference to the same patient record or
to the same test order record
This field indicates the comment
Comment
3 I A 1 origination point:
Source
I clinical instrument system
This field reports a phrase that describe
the comment; possible descriptions are:
♦ Unknown LIS Code
4 Comment Text I A 60 ♦ Data related to PID not accepted by
instrument system
♦ Data related to SID not accepted by
instrument system
This field indicates the comment type:
5 Comment Type I A 1 ♦ G generic/free text comment
♦ T test name comment

Legend:
I Ignored N Never
Example Comment Record (C)

Record sent by
C | 1 | I | Unknown LIS Code | G
Analyzer system

CHAPTER 08 - TROUBLESHOOTING GUIDE
CHAPTER 08
- TROUBLESHOOTING GUIDE -
PROBLEM POSSIBLE CAUSE/RIMEDY
THE SAMPLING PROBE DOES NOT The probe could be obstructed; plunge the probe.
ASPIRATE. If the diluter module works, check if there are
squeezed or torn tubing.
THE SAMPLING PROBE DOES NOT Check the valve of the diluter.
DISPENSE THE WASH SOLUTION Check if there is enough wash solution in the
INTO THE WASHING WELL bottle.
Check all tubing connection.
REPEATIBILITY OD RESULTS Sampling probe dirty.

INSUFFICIENT Hydraulic leak and/or air bubbles in the hydraulic
circuit (sampling).
Check sampling probe fit.
Check the fit of the hydraulic tubes and their
connection: if necessary replace the tubes and/or
adjust their connection fittings.
Deterioration of the Reagent(s)? If yes substitute
the bad reagent(s).
Photometer lamp not stable? If yes change the
lamp following the procedure described in
Chapter 06 – Maintenance.
Check the probe cleaner pad. It may be dirty. In
this case, change the probe cleaner pad.
Check the micro-flow cell, make sure it is clean;
if necessary, carry out the procedure described in
THE ASPIRATION INTO THE The aspiration probe may be dirty or obstructed.
MICRO FLOW CELL IS NOT The tubing may be torn or obstructed.
CONSISTENT Check that the probe descend enough below the
liquid level to correctly aspirate.
THE ASPIRATION ARM DESCENDS The silicon tubing of the peristaltic pump may be
ENOUGH BELOW THE LIQUID torn.
LEVEL TO CORRECTLY ASPIRATE The peristaltic pump rotor is too tight.
BUT DOES NOT ASPIRATE The probe may be obstructed or unhooked.

CHAPTER 08 - TROUBLESHOOTING GUIDE
THE WASHING WELL IS NOT The tubing from the washing well to the electro-
EMPTIED EVEN IF THE valve may be torn or not properly connected.
PERISTALTIC PUMP WORKS Check the electro-valve (of the aspiration circuit).
PROPERLY Check the peristaltic pump tubing.
ALL AUTOZERO VALUES ARE OUT The micro-flow cell may be empty. Fill it with
OF RANGE water.
The peristaltic pump may not be working
properly. Check if some tubing need to be
changed.
Check the aspiration probe and its connection
with the micro-flow cell. The may be some
leakage or air bubbles in the micro-flow cell, or
the probe may be dirty.
The wash solution bottle may be empty.
Check the peristaltic pump electro-valve.
Adjust the aspiration volume; there may be too
much or to little that has been aspirated.
The micro-flow cell is dirty or air bubbles get
into the cell.
Lamp is nearing the end of its life cycle or
premature deterioration. In both cases, change the
lamp following the procedure described in
THE RESULTS OF THE CONTROLS Make sure that the controls have been analyzed
ARE OUT OF RANGE with he methods specified by the manufacturer.
Make sure that the settings are correct for the
following parameters: wavelenght, temperature,
sampling volume, reagent volume, factor.
Make sure that the water used to prepare the
reagent and controls is either bi-distilled or
deionized water.
Check reagents, controls and standards Make sure
that they have been prepared in accordance with
the instructions given by the manufacturer.
Check that the recommended wash solution was
used and not simply distilled water.
KINETICS TESTS (PARTICULARLY The micro-flow cell may be contaminated. Clean
GOT OR GPT) GIVE LOW RESULTS. the micro-flow cell according to the procedure
described in Chapter 06 – Maintenance.
The reagent container may be contaminated.
The reagent may be expired or not fresh.
Check if the correct factor is used..
Check the volume settings.
Check the filter.
Check the temperature.

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VegaSys Service Manual Rev.01

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VEGASYS

Via E. Barsanti 17/A

Chapter 02 SYSTEM DESCRIPTION

Chapter 03 ELECTRICAL SCHEMES AND DRAWINGS

Chapter 04 DIAGNOSTIC PROGRAM

Chapter 05 SETTINGS & ADJUSTMENTS

Chapter 07 HOST COMMUNICATION

Chapter 08 TROUBLESHOOTING GUIDE

VegaSys Service Manual Rev.01 15 March 2009 Page 1

VegaSys Service Manual Rev.01 15 March 2009 Page 1

Upon consignment of the crate, take out the

the left side of the instrument. Rome-Italy

Open the crate/box from the top and very

MAKE SURE THAT UNPACKING IS CARRIED OUT BY TWO PEOPLE.

VegaSys Service Manual Rev.01 15 March 2009 Page 2

Ambient-climatic conditions for transportation, warehousing and storing the instrument

VegaSys Service Manual Rev.01 15 March 2009 Page 3

1.2 INSTRUMENT INSTALLATION

1.2.1 INSTALLATION SITE

USE Indoors and dry

1.2.2 ELECTRICAL POWER SUPPLY

VOLTAGE 100 : 230Vac 47/63Hz ±10%

VegaSys Service Manual Rev.01 15 March 2009 Page 4

PLEASE NOTE: IT IS IMPORTANT THAT THE LABORATORY’S ELECTRICAL POWER SOURCE BE

⇒ NO-BREAK MODULE (UPS - Uninterrupted Power Supply)

1.2.3 ACCESSORIES CONNECTION

1.2.3.2 Instrument-Computer connection

1.2.3.3 Liquid connections

VegaSys Service Manual Rev.01 15 March 2009 Page 5

1.2.4 LABELS AND SYMBOLS

CAUTION! READ INSTRUCTIONS IN THE OPERATOR MANUAL

VegaSys Service Manual Rev.01 15 March 2009 Page 6

Æ LIST OF CONSUMABLES AND ACCESSORIES EXCLUDED FROM WARRANTY:

1.2.6 REGULATORY COMPLIANCE

The Vegasys complies with:

1.2.7 LIMITATION OF USE

VegaSys Service Manual Rev.01 15 March 2009 Page 7

2.1 SYSTEM DESCRIPTION ............................................................................................2

2.2 MAIN COMPONENTS OF THE ANALYZER ..................................................2

2.2.1 ANALYTICAL PLATE ....................................................................................................2

2.2.2 SAMPLING SYSTEM .....................................................................................................3

2.2.3 SAMPLING ARM ............................................................................................................3

2.2.4 ASPIRATION ARM .........................................................................................................5

2.2.5 PROBE CLEANER ..........................................................................................................5

2.2.6 WASHING WELL............................................................................................................5

2.2.8 PERISTALTIC PUMP......................................................................................................5

2.2.9 PHOTOMETRIC SYSTEM..............................................................................................6

2.2.10 MICRO-FLOW CELL .....................................................................................................7

2.3 COMPUTER AND SOFTWARE FEATURES ......................................................8

2.4 DIMENSIONS AND WEIGHT .................................................................................8

2.5 TECHNICAL AND OPERATIVE FEATURES ...................................................9

VegaSys Service Manual Rev.01 15 March 2009 Page 1

2.1 SYSTEM DESCRIPTION

2.2 MAIN COMPONENTS OF THE ANALYZER

The basic operating cycle takes 18 seconds.

VegaSys Service Manual Rev.01 15 March 2009 Page 2

Fig. 1 – Analytical plate

2.2.2 SAMPLING SYSTEM

2.2.3 SAMPLING ARM

VegaSys Service Manual Rev.01 15 March 2009 Page 3

Aspiration arm Analytical plate

Fig. 2 – Sampling arm and aspiration arm

Fig. 3 – Probe cleaner

VegaSys Service Manual Rev.01 15 March 2009 Page 4