Pangasinan State University

BAYAMBANG CAMPUS
Zone VI, Bayambang, Pangasinan

CELL and MOLECULA BIOLOGY

Sci 121 Laboratory
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Name: Score:
Year and Section: Date:
Laboratory Activity No. 7
DNA EXTRACTION

Materials
250 ml bottled water
3 plastic cups
Clear dishwashing liquid
1 tbsp salt
100 ml isopropyl alcohol
Food coloring (any color)
1 vial (optional)

Procedure
1. Make a saline solution in a plastic cup by mixing 1 tbsp of salt and 250 ml water.
2. Gargle the saline solution for 1 minute.
3. Spit the saline solution into a new cup.
4. Add 1 drop of the dishwashing liquid into the saline solution and stir gently by swirling
the cup..
5. In a separate cup mix the isopropyl alcohol and 3 drops of food colloring.
6. Gently pour the alcohol mixture into the saline solution little by little until you see white
clouds and strings form.
7. You can transfer your DNA in avial and preserve it.

I. Answer the following questions.

1. What is the use of gargling saline solution in the experiment?

2. What is the importance of the dishwashing liquid in the experiment?

 Pangasinan State University
BAYAMBANG CAMPUS
Zone VI, Bayambang, Pangasinan

CELL and MOLECULA BIOLOGY

Sci 121 Laboratory
------------------------------------------------------------------------------------------------------------------------------------------

3. Why does DNA form when isopropyl alcohol is added?

Figure 1. (Picture of your DNA)