Lyceum -Northwestern University

Tapuac District, Dagupan City, Pangasinan

College of Arts and Sciences
Natural Sciences Department

“Photographic Atlas: A Compilation of Laboratory Activities in

Biochemistry”
Bachelor of Science in Nursing
1_J
S.Y. 2022 – 2023

Submitted By:

Last Name, First Name and Middle Initial

Submitted To:

Mr. Mark Joseph B. Escobal

Instructor

Grade:
 Table of Contents

Title page …………………………………………………………………………………………………………………………

Table of contents ………………………………………………………………………………………………………
Introduction …………………………………………………………………………………………………………………… Error:
Reference source not found
Acknowledgement …………………………………………………………………………………………………………… iv
Members of the Group ……………………………………………………………………………………………… v
Lab. Activity #1: pH and Buffer ……………………………………………………………… vi
Introduction
Materials
Procedure
Result and Conclusion
Lab. Activity #2: Osmotic Behaviors of RBCs ………………………………… vii
Introduction
Materials
Procedure
Result and Conclusion
 Introduction
This photographic atlas is a compilation of different
laboratory activities . . . . . .
 Acknowledgement
The group take this opportunity to gratefully acknowledge the
assistance and contributions of their fellow students for making
this laboratory activities possible. Perseverance, dedication and
unwavering supports- the underlying principles that made us pursue
these activities.
A top to these, for the blessed wisdom and strength that our
Almighty God had bestowed us, we like to inflict our sincerest
gratitude. We also commend the untiring supports from our dearest
parents who are always there to understand us and shine a guiding
light to the path of success, and sustain our financial needs.
The group would like to express their great appreciation to
Professor Mark Joseph B. Escobal for the sincere guidance,
motivating, and enthusiastic encouragement as well as skills in
laboratory we received to uphold our activities.
 Members of the Group

Standing from L-R: dela Cruz, Juan, Paterno, Pedro……..

Sitting from L-R: Sanchez, Juana Font size: 9
 Laboratory Activity #1
pH and Buffer

Introduction (one paragraph is enough)

This laboratory experiment/activity will tests . . . .
Generally, pH BUFFERS are composed of a mixture of a conjugate
base with a weak acid which can absorb excess hydrogen atoms
to maintain a stable pH value in solution. They are
subsequently ideal for calibrating electrochemical
potentiometers for low uncertainty pH measurements with
greater traceability.

Materials: (include pictures of your materials and equipment)

Milk-6
EggWhite-9
Vinegar-3
Coke-3
Pepsi-3
Coffee-5
Calamansi Juice-3
Toothpaste-7
Shampoo-5
Soap Solution-7
Rubbing Alcohol-5
Limewater-6

Procedures: (include pictures in step-by-step format)

First is the Antilog pH, Before you can get it you need to
convert the pH value obtained above to hydronium ion
concentration. Next is the Urine, the 3 ml of urine add 3
drops of bromyhmol and next is the 3 ml of urine with a 3
drops of bromcresol last is the 3 ml of urine with 3 drops of
thymol. if the color is intermediate between yellow and deep
blue, no further rough test is necessary.

Result and Conclusion (with pictures)

Based on the result of our test is the bromthymol turned yellow and
it has a pH value of 6 and the bromcresol turned deep blue and it's
pH value is 7.6 next is the thymol it turned deep yellow. So the
result of the urine is acidic and the average pH of the urine is pH
6.
 Laboratory Experiment #2
Osmotic Behaviors of RBCs

Introduction

Materials

Procedures

Result and Conclusion

 Laboratory Experiment #2
Denaturation OF Proteins

Introduction
This laboratory experiment will tests the Nature/Organization
of protein molecule and also the properties of proteins. The
test that are used in the experiment will show the change in
the protein based (that is used) in different types of
testing.

Materials
2 pieces of egg
Water
Concentrated Nitric Acid
Egg Albumin Solution
Concentrated Hydrochloric Acid
Concentrated sulfuric Acid
Cupric Sulfate Solution
Lead Acetate Solution
Mercuric Chloride
Trichloroacetic Acid
Picric Acid
Phosphotungstic Acid
Chloroform
Test Tubes
2 Test Tube Rack
Funnel
Pipet
Beaker
Graduated Cylinder

Procedures
a. Coagulation of Proteins

1. By Heat

Place 3 ml of egg albumin solution in a test tube and boil

gently for a few minutes. Describe the change in the albumin
solution.
 Laboratory Experiment #2

Result: after heating for a few minute

the solution formed bubbles which is
positive solution.

2. Heller’s Ring Test

Place 1 ml of conc. Nitric acid in a test tube. Dilute 1 ml of egg

albumin solution with 1 ml of water and mix well. Incline the test
tube containing the nitric acid and carefully pour the 3 ml of
dilute albumin solution down the side of the tube so that it forms a
layer above the acid solution. What do you observe at the juncture
of the two liquids

Result: The nitric acid cooked the

egg and separated it the three
solution got separated with each other
and formed a cloudy white ring.
 Laboratory Experiment #2

3. By Alcohol

Place 1 ml. of egg albumin in a test tube and add 1 ml. of 95%

Result and Conclusion