PATIENT'S NAME : MRS. SHINDE RUCHA A. CLIENT : P.H.

PUNE
AGE / GENDER : 30 Years / Female REGISTRATION DATE : 12/01/2022 11:30am
DOB. : 28-June-1991 SAMPLE COLL. DATE : 12/01/2022 02:59pm
REFERRED BY DR : SELF ACCESSION DATE : 12/01/2022 03:43pm
PATIENT ID : 132008260 AUTHENTICATION DATE : 12/01/2022 07:37pm
SAMPLE COLLECTED BY : HOME COLLECTION
*132008260* PASSPORT NO. :

COVID-19 RT-PCR

SARS-COV 2 (COVID -19) detection by Qualitative Real Time PCR

RESULT NEGATIVE

SPECIMEN TYPE : Nasopharyngeal Swab and Throat Swab

Interpretation of the results: Target considerate for analysis of SARS CoV-2 are, N Gene and ORF1ab. Test is
considered positive if both SARS CoV-2 targets are detected.

ICMR Lab Registration Number: PHDIACENPMH

A) INTERPRETATION GUIDELINES:

For result as "POSITIVE"

1. Indicates presence of SARS-CoV-2 in the specimen provided.
2. A positive result does not distinguish between a viable/replicating organism and a non-viable organism

For result as "NEGATIVE"

1. Indicates absence of SARS-CoV-2 virus in the given specimen. However, it does not rule out the infection completely
and should not be used as the sole basis for making decisions related to treatment and other patient management.
2. Repeat sampling and testing of lower respiratory tract specimens is recommended in severe or progressive disease.
The gap of 2-4 days from first specimen should be considerate for repeat testing if suggested by authorized registered
medical practitioners.
3. If a subsequent test is tested positive, it may indicate an infection acquired subsequently or increase in viral load to
detectable level after the first test.
4. "Negative" result may be seen due to -
a. Test done too early or too late where the viral load is below detection limit.
b. Improperly collected and stored specimen.
c. Viral mutations

B) PATIENT INSTRUCTIONS:

1. Test will be done only if valid prescription of authorized registered medical practitioners or concerned state
authority doctors is available.
2. Kindly consult referring Physician/ Authorized Govt. hospital for appropriate follow up.
3."Positive" status needs to be notified to the all the local /Government authorities/ appropriate authorities as per
the existing rules/regulations.
4. The results are pertaining to the specimen tested and clinicalcorrelation with patient history, radio logical findings and
other co-infections is necessary to be determined.
5. Lower detection limit of the assay is 10 GCE/ reaction.

C) NOTE :

1.This test is conducted with a kit approved by ICMR.

2.All approved kits being used also may have different positive and negative predictive values leading to mismatch of
results.
3.For Positive results figures in next column in front of the Target Genes denotes the Ct value (cycle threshold) for

DR.SALMA PATWEGAR
* Not in NABL Scope # Referred test
Reviewed By :DR.NAMITA RAYTEKAR MD Microbiology
Reg. No. : 2012/04/0950
Page 1 of 2
PATIENT'S NAME : MRS. SHINDE RUCHA A. CLIENT : P.H.PUNE
AGE / GENDER : 30 Years / Female REGISTRATION DATE : 12/01/2022 11:30am
DOB. : 28-June-1991 SAMPLE COLL. DATE : 12/01/2022 02:59pm
REFERRED BY DR : SELF ACCESSION DATE : 12/01/2022 03:43pm
PATIENT ID : 132008260 AUTHENTICATION DATE : 12/01/2022 07:37pm
SAMPLE COLLECTED BY : HOME COLLECTION
*132008260* PASSPORT NO. :

COVID-19 RT-PCR
that gene. Cut off, Ct for the assay is >/= 37.
4.This test is qualitative assay &quantification of viral load is not done. Ct values can be affected by variability in sample
collection /site, techniques and kit used by laboratories. So, Ct values are not an absolute indication for viral load
and severity of disease. Thus, it shouldbe interpreted with caution.
5.Ct values more than 30 is considered as borderline cases which should be confirmed with a repeat new specimen after
three to four days if clinically indicated. Borderline positive cases may give variable results on repeat testing.
6.REPORTING CONDITIONS: It is presumed that the test performed on the specimen belong to the patient name
oridentified.
7.Test results may vary from laboratory to laboratory, and should be interpreted by referring doctor only. Only such
medical professional having understanding of reporting units, reference ranges and limitations of technologies should
interpret results.

D) REFERENCES :

1. ICMR - https://www.icmr.gov.in/
2. Kit Insert: CoviPathTM COVID-19 RT-PCR Kit; Doc. Part No. 100099241; Pub. No.MAN0024710; Rev.C

E) ABREVATIONS :

1. SARS CoV-2 - Severe Acute Respiratory Syndrome - Coronavirus -2

2. ICMR - Indian Council of Medical Research
3. RT- PCR- Reverse Transcriptase Polymerase Chain Reaction.
Note : As per Pune Municipal Corporation letter No - 5869 dated on 06/08/2021, it is mandatory to
download PMC Home isolation App for Covid-19 RTPCR Positive patients.
-------------------------------------------------------------------------------------------------------------------------------------------------
***End of Report***

DR.SALMA PATWEGAR
* Not in NABL Scope # Referred test
Reviewed By :DR.NAMITA RAYTEKAR MD Microbiology
Reg. No. : 2012/04/0950
Page 2 of 2