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1
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Round, oval, or boat-shaped cells with translucent basophilic cytoplasm observed greatest at the center
of the cell and with peripheral nuclei are examples of:
Select one:
a. Endocervical cells
b. Intermediate squamous cells
c. Mature superficial squamous cells
d. Parabasal cells
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The correct answer is: Intermediate squamous cells
Question 2
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Which of the following is NOT a purpose of mounting?
Select one:
a. Mounting may prevent the permanent keeping of slides
b. Mounting may protect the tissue from physical injury
c. Mounting may protect the tissue stain from bleaching
d. None of the above answers are correct
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The correct answer is: Mounting may prevent the permanent keeping of slides
Question 3
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What should be done to cytologic specimen with excessive red blood cells that are not needed for the
examination?
Select one:
a. Centrifuge the specimen and analyze the supernatant
b. Add acetic acid to the specimen before fixation
c. Process the specimen as usual using the routine technique
d. Discard the specimen and recollect
e. Centrifuge the specimen and analyze the concentrated cell
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The correct answer is: Add acetic acid to the specimen before fixation
Question 4
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Which of the following decalcifying agents is NOT recommended for the nuclear study of osteocytes?
Select one:
a. Perenyi’s fluid
b. Trichloroacetic acid
c. EDTA
d. Von Ebner’s fluid
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The correct answer is: Perenyi’s fluid
Question 5
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What is the main difference between freeze drying and freeze substitution methods?
Select one:
a. Freeze drying uses vacuum while freeze substitution uses Rossman fluid
b. Freeze drying uses Rossman fluid while freeze substitution uses vacuum
c. Freeze drying requires dehydration of tissues while freeze drying does not
d. Freeze substitution requires dehydration of tissues while freeze drying does not
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The correct answer is: Freeze drying uses vacuum while freeze substitution uses Rossman fluid
Question 6
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What should be done to prevent damage to the organic substances of the tissue by the acid decalcifying
agents?
Select one:
a. Dilute the decalcifying agent with normal saline solution
b. Adequately fix the tissue
c. Increase the concentration of the decalcifying agent
d. Replace the decalcifying agent with EDTA
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The correct answer is: Adequately fix the tissue
Question 7
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What is the fixative used to make cell blocks?
Select one:
last page )
a. Heat
b. Methyl alcohol
c. Formalin
d. Ethyl alcohol
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The correct answer is: Formalin
Question 8
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The coverslip of a permanently mounted slide can be removed by:
Select one:
a. Freezing the slide overnight
b. Exposing the slide to water vapor
c. Soaking the slide in xylene
d. Covering the mounted slide with a mixture of 6 parts butyl acetate and 1 part Durofix
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The correct answer is: Soaking the slide in xylene
Question 9
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A maturation index of 0/95/5 is indicative of:
Select one:
a. Estratrophy
b. Birth
c. Lactation
d. Childhood
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The correct answer is: Birth
Question 10
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Which of the following protocols is NOT recommended for decalcification method?
Select one:
a. Rapid agitation
b. None of the above
c. Smaller pieces of tissue
d. Higher volume of decalcifying agent
e. Processing at 18-30C
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The correct answer is: Rapid agitation
Question 11
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Predominant normal vaginal flora responsible for preventing opportunistic infections by providing an
acidic environment:
Select one:
a. Lactobacillus acidophilus
b. Staphylococcus aureus
c. Trichomonas vaginalis
d. Gardnerella vaginalis
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The correct answer is: Lactobacillus acidophilus
Question 12
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In glycerin jelly mounting medium, the purpose of phenol crystal is to:
Select one:
a. Preserve the solution
b. Act as a solidifying agent
c. Increase the refractive index
d. Dilute the gelatin and glycerol
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The correct answer is: Preserve the solution
Question 13
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The presence of spermatozoa in the Pap smear of the patient is considered pathologic. This statement
is:
Select one: ↳ normal
a. False
b. True
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The correct answer is: False
Question 14
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All of the following are specimen that can be processed by cell blocking, EXCEPT:
Select one:
a. Vaginal secretions
b. None of the above
c. Thoracic fluid
d. Sputum
e. Bronchial aspirate
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The correct answer is: Vaginal secretions
Question 15
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During the microscopic examination of a calcified tissue, the pathologist noticed that the tissues
surrounding the supposed area of the calcified matter are either destroyed or missing. Based on this
finding, the best possible explanation is:
Select one:
a. Dehydration of the tissue was performed after decalcification
b. The tissue was not fixed properly
c. The concentration of the decalcifying agent was too low
d. Freezing of the tissue is too slow
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The correct answer is: The tissue was not fixed properly
Question 16
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If mounting will be delayed, stained tissue slides must be left in xylene. This statement is:
Select one:
a. False. Stained tissue slides must be left in tap water to remove excess xylene
b. False. Stained tissue slides must be removed from xylene and stored in the refrigerator
c. True
d. False. Stained tissue slides must be removed from xylene immediately
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The correct answer is: True
Question 17
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Which of the following statements regarding Doderleins bacillus is INCORRECT?
Select one:
a. Increase in number of the bacteria is always considered pathologic
b. The presence of bacteria maintains the normal flora in the vagina
c. The bacteria is the cause of the low pH in the vagina
d. None of the above answers is correct
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The correct answer is: Increase in number of the bacteria is always considered pathologic
Question 18
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Using the maturation index, the only cell that can be seen in the specimen of a woman without ovaries
is:
Select one:
a. Parabasal cell
b. Intermediate cell
c. Basal cell
d. Mature superficial squamous cell
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The correct answer is: Intermediate cell
Question 19
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Exfoliative cytology is useful in:
Select one:
a. Diagnosing cancer
b. Determining the prognosis of a disease
c. Estimate the age group of the female
d. All answers are correct
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The correct answer is: All answers are correct
Question 20
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Which is considered as the fastest decalcifying agent?
Select one:
a. Formic acid
b. Electrophoresis
c. Nitric acid
d. Chelators
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The correct answer is: Nitric acid
Question 21
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Knob-like structures found in the inner nuclear membrane of most somatic cells which denote genetic
sex:
Select one:
a. Chromatin
b. Neither
c. Both
d. Barr bodies
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The correct answer is: Both
Question 22
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Canada balsam, clarite, xam, and DPX are examples of which type of mounting medium:
Select one:
a. Temporary mounting medium, except for Canada balsam
b. Permanent mounting medium, except for clarite
c. All are permanent mounting medium
d. Permanent mounting medium, except for xam
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The correct answer is: All are permanent mounting medium
Question 23
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The quantitation method that examines the relation of the similar cells examined maturation index but
in reversed order is called:
Select one:
a. Femininity index
b. Acidophilic index
c. Crowded cell index
d. Karyopyknotic index
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The correct answer is: Femininity index
Question 24
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Which of the following mounting medium is recommended for a tissue stained by a dye that is soluble to
alcohol or xylene?
Select one:
a. Canada balsam
b. Water
c. Glycerin jelly
d. DPX
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The correct answer is: Glycerin jelly
Question 25
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Which is considered as the fastest decalcifying agent?
Select one:
a. Negative electrode
b. Phloroglucin-nitric acid
c. EDTA
d. Formic acid-sodium citrate solution
e. Aqueous nitric acid solution
Feedback
The correct answer is: Phloroglucin-nitric acid
Question 26
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Which of the following statements regarding decalcification is TRUE?
Select one:
a. The fastest decalcifying agent is a chelator
-
- mini acid
b. Tissues must be fixed with formalin for at least 2 days
c. Before dehydration, tissues decalcified with trichloroacetic acid must be washed water X no washing out
-

d. Negatively charged calcium ions is removed faster during electrophoresis por i truly
-
-

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The correct answer is: Tissues must be fixed with formalin for at least 2 days
Question 27
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Measuring the degree of decalcification using strong ammonia is not useful in:
Select one:
chemical test
a. Chromic acid not compatible
b. EDTA
-

if
c. Aqueous nitric acid
d. Formic acid
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The correct answer is: Chromic acid
Question 28
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The best way to resolve excessive mounting medium oozing out at the edges of the coverslip is:
Select one:
a. Remount the slide
b. Soaking the slide in xylene
c. Wiped it with cloth soaked with xylene
d. Use other solvent such as alcohol
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The correct answer is: Wiped it with cloth soaked with xylene
Question 29
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Tissue slides can already be examined microscopically immediately after mounting. This statement is:
Select one:
a. True
b. False. The mounted slides must be placed on a hot plate for up to 2 hours before examination
c. False. The mounted slides must be placed in a wax oved for up to 2 hours before examination
d. False. The mounted slides must be left at room temperature for 2 to 3 days before examination.
e. Any of the answers is correct, except A
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The correct answer is: True
Question 30
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Which of the following materials of histotechnique is used last?
Select one:
a. Ethyl alcohol
b. Strong ammonia
c. Aqueous nitric acid
d. Neutral buffered formalin
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The correct answer is: Ethyl alcohol
Question 31
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To accelerate and ensure complete mounting of tissue slides with coverslips, the mounting media
should be miscible with:
Select one:
a. Water
b. Xylene
c. Paraffin
d. Alcohol
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The correct answer is: Xylene
Question 32
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The excess xylene on the tissue must be wiped dry with:
Select one:
a. Clean, dry, fine cloth the tissue must not be Nbd
b. Clean, dry, soft tissue out
c. Dry cloth soaked with alcohol for it croakpommy come
d. Soft tissue or fine cloth
e. None
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The correct answer is: None
Question 33
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If most synthetic mounting media become too thick, they may be thinned with:
Select one:
a. Methyl alcohol
b. Xylol
c. Ethyl alcohol
d. Ether
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The correct answer is: Xylol
Question 34
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Cells that are normally found and abundant during two weeks of age to puberty, after childbirth, and
after menopause:
Select one:
a. Parabasal cells
b. Endometrial cells
c. Navicular cells
d. Endocervical cells
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The correct answer is: Parabasal cells
Question 35
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During the process, decalcifying solutions should be changed:
Select one:
a. Every hour
b. Every minute
c. Every week
d. Every day
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The correct answer is: Every day
Question 36
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Using the chemical method, the completeness of decalcification is indicated by:
Select one:
a. Clear solution
b. Red color in blue litmus paper
c. Blue color in red litmus paper
d. Cloudy solution incomplete
-

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The correct answer is: Clear solution
Question 37
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Cloudy areas in a mounted slide can be solved by:
Select one:
a. Removing the excess xylene before mounting
b. Repeating the dehydration process
c. Sealing the edges of the coverslip
d. Drying out the tissue before mounting
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The correct answer is: Repeating the dehydration process
Question 38
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Which of the following statements CORRECTLY characterizes a good mounting medium?
Select one:
a. It should dry quickly to avoid delays in microscopic examination
b. It should set hard to maintain the condition of the tissue
c. To maintain the color of the tissue, the harden state of the mounting medium should have a pH that is
higher that its liquid form
d. All of the above
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The correct answer is: It should set hard to maintain the condition of the tissue
Question 39
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Which of the following is the correct interpretation of Pap smear if the result is shows atypical cytology
but no evidence of malignancy?
Select one:
a. Class 0
b. Class III
c. Class IV
d. Class II
e. Class I
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The correct answer is: Class II
Question 40
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Xylol in routine Pap staining is used to:
Select one:
a. Deparaffinize tissues
b. Dehydrate tissues
c. Clear tissues
d. A and B answers are correct
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The correct answer is: Clear tissues
Question 41
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To avoid distortion of the image during microscopy, the refractive index of the mounting medium should
be near to the refractive index of:
Select one:
a. Air between the tissue slide and objective
b. Glass slide and coverslip
c. Water solvent of the mounting medium
d. The tissue
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The correct answer is: Glass slide and coverslip
Question 42
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Carnoys fluid is useful for blood specimen because:
Select one: →
a. It lyses RBCs alcohol
b. It coagulates blood cells
c. It preserves RBC well
d. It is the fastest fixative
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The correct answer is: It lyses RBCs
Question 43
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Decalcification is performed after:
Select one:
a. Impregnation
b. Gross examination
c. Clearing
d. Fixation
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The correct answer is: Fixation
Question 44
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Which of the following is the correct interpretation of Pap smear if the result shows conclusive
malignancy?
Select one:
a. Class V
b. Class III
c. Class IV
d. Class I
e. Class II
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The correct answer is: Class V
Question 45
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Barr bodies are observed in the squamous cells of:
Select one:
a. Male
b. Female
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The correct answer is: Female
Question 46
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All of the following are criteria of a normal pregnancy, EXCEPT:
Select one:
a. Absence of ferning
b. High number of intermediate cells
c. High number of parabasal cells
d. Abundance of Lactobacillus acidophilus bacteria
e. High number of pregnancy cells
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The correct answer is: High number of parabasal cells
Question 47
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Question text
Ferning is produced when:
Select one:
a. Progesterone is increased
b. Estrogen is increased
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The correct answer is: Estrogen is increased
Question 48
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Exfoliative cytology is limited only to the study of cells scraped off from the vagina and cervix. This
statement is:
Select one:
a. False
b. True
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The correct answer is: False
Question 49
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Pap smear specimens that have more than 75% of cells covered by RBCs and WBCs are considered
unsatisfactory specimens. This statement is:
Select one:
a. False
b. True
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The correct answer is: True
Question 50
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Fixing the cytologic smear for 30 minutes and mounting it with a coverslip using glycerin is a method
used when the specimen is:
Select one:

mailing
a. Gastric fluid
b. To be transported
c. To be stored permanently
d. To be processed for routine examination
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The correct answer is: To be transported