University of Santo Tomas

Faculty of Pharmacy
Department of Medical Technology
Full Enriched Virtual Mode
Clinical Faculty

SN: 43 Name: REYES, Danna Angelick G.

Section: 4PMT Date: Febuary 21, 2022
GRIFOLS Wadiana and Erytra Activity

Instruction: Answer the following guide questions comprehensively.

1. What does “100% use of cards“ & optimized reagent consumption mean? Do you consider
this feature as advantage of automated machine for blood banking? Explain your answer
(5pts)

In terms of defining the ‘100% use of cards’, it implies that the machine only uses the
columns needed for testing, This can pierce through one column at a time. Other machines
perform piercings by threes (they pierce 3 columns of the card at a time), this implies that they
can either decide to use the excess columns or they would be wasted since the gel would dry
up if left to sit unused. Furthermore, an optimized reagent consumption means that the
machine would dictate exactly how much of each reagent you would need to use for a specific
number of tests. The machine calculates the volume of reagent you would require to perform
certain tests. This is advantageous to the laboratory since this would minimize the amount of
reagent wasted. This would also prevent any errors that may occur when you add an
inadequate amount of reagent.

2. How can automated machines in blood banking reduce the possibility of errors to ensure
safety throughout processing? (5pts)

Since these machines are fully automated, this would help in negating the possibility
of human errors from happening. The user would simply load the reagents and samples into
the machine, the rest is automatically done by the machine. However, we must always
ensure the proper calibration of the machines when testing. Features such as the probe
impact detector can check whether there is an obstruction, this will then trigger the alarm and
alert the operator. The Wadiana also features Clot Detection which aids in reducing the
occurrence of erroneous results. Lastly, the machine captures a photo of the gel card for the
operator to view and review and validate the results.

3. Give the difference between the two machines using the following tabular format. (14pts)

Category Grifols Wadiana Grifols Erytra

Instrument Capacity The loading Capacity is 18 reagents 200 cards can last up to 1,600 tests, 72
which is composed of 16 reagent vials samples, 46 liquid reagents. Centrifuge
and 2 diluents, 48 samples, and 24 DG Capacity is at 24 DG Gel® Cards.
Gel® Cards Centrifuge Capacity is at 12
DG Gel® Cards.
Throughput 25 ABO/hr Maximum throughput: 100 cards/hr,
ensuring a 800 results/hr.
Full ABO/Rh blood group typing: 66
samples/hr.
Antibody screening (I, I1, III, IV): 147
samples/hr.
Operational Security → Automatic dispensing verification → Password-controlled system access.
by imaging of all wells.Positive → Automatic Database Backup
identification of samples and function.
reagents. → Dedicated management of Quality
→ DG Gel® cards integrity Control Results.
checking. → View warnings in real-time.
 University of Santo Tomas
Faculty of Pharmacy
Department of Medical Technology
Full Enriched Virtual Mode
Clinical Faculty
→ Direct card piercing to prevent → Certain roles are configurable for
cross contamination. each user with different
→ Continuous monitoring of levels permissions.
of reagents, diluents and wash
and disposal solutions.
→ Secured verification of reagents'
batch compatibility and expiry
control.
Traceability → Enhanced bidirectional→ System access is password
communication with the hospital controlled.
central computer (LIS). → The entire process and the results
→ Password-controlled access. are fully traceable.
→ It is not necessary to do manual → Complete result traceability report
data entry. which includes the analyzer, the
→ Hemovigilance software users, the information on reagents,
connections (BLUE Grifols, and the various modifications
Gricode). applied.
→ Database access from any
computer with an internet
connection. This will provide proper
validation of results
→ Monitor the status of the
autoanalyzer and the samples.
→ Bidirectional connection to the LIS.
→ Communication with other Grifols
Systems (Wadiana, DG Reader).
→ It is possible to work with several
interconnected Erytra units
Software WADiana 8XT Software, WADiana® Erytra Eflexis Software, AB ID Interpretation
Compact Software Advanced Version Software, and Windows 7 OS.
Flexibility and Adaptability → Automatic sample tube diameter → Provides a more Flexible
detection. performance.
→ More flexible management of → Smart and Compact design.
emergency samples with easy → Intuitive operations, which includes
application of DG Reader to the continuous and random, loading and
system. unloading of cards, samples, and
→ Network connection between reagents.
different WADiana® units for → Flexible incubator management
more efficient throughput, available depending on the test.
functional random access and → STAT management
better loading accessibility. → The machine only requires you to
→ Random positioning of samples load the samples in any position.
& reagents. The samples are automatically
→ Multi Diameter tube loading prioritized when you click the
without adaptor. “STAT” button.
→ Convenient Universal Reagents and
Wash Solutions.
→ Machine also features Optimal
Direct Drainage which maximizes
the system’s autonomy
→ Exclusive simultaneous perforation
and dispensing technology allows
for the 100% use of the card wells
and helps prevent cross
contamination.
→ Reduce hands-on operation time
with an intuitive experience,
including innovative system fluid
loading & unloading
→ Minimal Maintenance required.
 University of Santo Tomas
Faculty of Pharmacy
Department of Medical Technology
Full Enriched Virtual Mode
Clinical Faculty
Operations Sample dilution: List of techniques:
→ Procedures such as dispensing
serum and/or plasma.
→ Red blood cell suspension
dispensing.
→ Reagent dispensing.
→ Incubation of cards (RT and
37°C).
→ Centrifugation of cards.
→ Digitized results reading.
→ Results interpretation.
→ Report generation.
→ Minimal training needed to use the
machine.
→ Software interface is easy to use
and is customizable based on the
needs of the laboratory
→ Full ABO/Rh blood group typing
(forward and reverse) - Rh + Kell
phenotype - Weak D - Crossmatch
test - Unexpected antibody
screening - Identification of
unexpected antibodies - Enzyme
assays - Direct Coombs' test -
Determination of special antigens -
Self diagnostic

List of techniques:
→ Full ABO/Rh blood group typing
(forward and reverse)
→ Rh + Kell phenotype - Weak D
→ Crossmatch test
→ Unexpected antibody screening
→ Identification of unexpected
antibodies
→ Enzyme assays
→ Direct Coombs' test
→ Determination of special
antigens - Self diagnostic

4. Create a flow chart on how to process a Crossmatching in Grifols Wadiana. (10pts)

 University of Santo Tomas
Faculty of Pharmacy
Department of Medical Technology
Full Enriched Virtual Mode
Clinical Faculty

5. Grade and Interpret the following Gel Cards using this format (6pts) e.g.

Test Agglutination Grade Interpretation

AHG.CD 2+ POSITIVE
AHG IgG 1+ POSITIVE
AHG C3d 2+ POSITIVE
AHG Control - NEGATIVE

6. Grade and Interpret the following Gel Cards using this format (5pts)

Test Agglutination Grade Interpretation (Blood Type)

A mf The test is deemed to be valid
B - since the Control is negative.
However, the presence of
D mf Mixed-field reactions
Control - necessitates that we should
repeat the test to verify the
results.
 University of Santo Tomas
Faculty of Pharmacy
Department of Medical Technology
Full Enriched Virtual Mode
Clinical Faculty
Department of Med

B.

Test Agglutination Grade Interpretation (Blood Type)

A -
B -
D 4+ Blood type O+
Control -