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  • MỘT SỐ KIẾN THỨC CƠ BẢN VỀ CHIẾT XUẤT DƯỢC LIỆU (tiếp)

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    36 views4 pages

    MỘT SỐ KIẾN THỨC CƠ BẢN VỀ CHIẾT XUẤT DƯỢC LIỆU (tiếp)

    Uploaded by

    Phạm Phát

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    © All Rights Reserved
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    ‘19 26/08/2023 MOT SO KIEN THUC CO BAN VE CHIET XUAT DUC LIFU (0p) - Tin chuyén mtn & NCKH - Khoa Duye - Bai hoe Duy Tan Trang chi Hei dap Kb hogeh tt nahi Thong bso ‘Thai kinda bibu Leh tht du miu 'Bpi nga gidng wien Tin ehuyén mén & NCKH Cun Pyar ntps:kduoe.cuytan edu.vdHoma/ArcloDetailvn/78/3887imot-so-kenshue-co-ban- . 2 MGT SO xaW THUC Co BAN VE catéT xUKT DUGC LIEU (tgp) MOT SO KIEN THUC CO.BAN VE CHIET XUAT DUOC LIEUILe) 414. Qus trinh thd tb ‘hat nig: Qu tri tm thf a us tinh Kuch tan gia hal pha léng aqua mt mang e8 tn ct bn thm, © ght la mang a6 chi cho dung maid qua ma Kg cho ct tan a qua. Mang goilé ming ban thé. Do dp ye thd thu cia cdc phan ti chét tan, dung mB s duce thdm tr pha ling c& néng 6 chSt tan hp hom sang pha ling 6 nding 48 cao hon, cho én Kh ape thy tnh can bing vol ap ly thm thd {Gng dung: trong i ba0 due Hou cadtnquyén sh ction cht ban thm, v vBy Kh duge gu cn tei, do tbe dyng cla chit nguyén sinh ma chi ed clung mal due tm vot bao lam cho duge bub tong né,cén cht tan trong t bao th ng Khuen tan ra nga8i duge. Do dé rg chit xu, ngué ta pha tim cc pha hy ct nguyén sina bing ri ogc bing cn ab thy hign qua inh chi ut 412. Quaitrinhthém ich ‘nig: qua rin hdr ich Ia qua win khuch tn ida hal ha lng qua mt mang c tiny chit hm ch, 6 nga ‘mang Khdng ch cho dung mb: qua ma cn cho echt an qua, nhung hl cho qua hing ent opin tr nh {ng dung: mang t B30 duoc leu 8 tinh cht cia mot mang thm ich, do 63 kh chit xubtndu mang té bao con iguyén vgn thi chi eb cht tan a phn tinh vation (hn ln la bast hd) Khudch tan qua mang thao; cén cc cht <6 Pisin trem (thing a ent keo, chttap..) tl kndng que duce mang if bio nn khéng b cht vao och cht. Nn vy, 128 thd coi mang tf ne nhu met mang Ine cin chon Ige- Ay cnn la wu adm cia mang té bao Ab voi gua inh cht xt Do 4, rong qua nh chit xu, ehdng nén xay cg ligu qua min; vl Kh dé mang t 8806] pha v6, tinh chon le tia ming th pao kong edn, cn ci 8 inn tp, gy ke kvm eho quan nh oh, 2 NhOmg ybu t& anh hung dén qué tinh chidt xubt due liga 2.4. Nhimg yu tb thuge vd than phan, eu tgo cua duge Hu 2.4.4. Mang té bio duge itu ‘Mang t6 bao due iu cd ns hating nib an qua tinh khubch tn. Khi edn sng, 46 no xy a qu tinh a0 ob {Ste tins chon ge Kh nt, 6 8 nol xy rae ign tng Kh tn, thm thu, thm ten, . Mngt bdo eeu to king dn gn, © he bj hay ain cht vt va thanh phn no noe ab cp ang wo nag che phn inn ajo gt mand dim nhign (hoa @5, ha khodng, pi sp.) Ng su thay 65 nay 06 th xy ra tng phn hogetoan pnén {ming t bdo va thrdng thay ab nibu & thee vt 6B 9 ‘i ve hye vl edn non hay mang mm nu & ey, hoa Ia thanh phn cia mang té bo ch yu 6 colse. Cellulose 6 tinh chit khong tan rong nese vi kg tan trong cbc dung mG kh, bn vong nhl a cao, 6 tinh mm déo gan 1B. BSI ver dre tol nay, dung mB Ys vo due iu, do eh en xay 1h cue iB NB xay mip, dB kno tho niu tap vio eh ei, i ture bud gi, rn ncn ha, 9B vb tn. Ui mang U8 bao rn ty vob th xy ea nog bib ah Mang tbo 66 h8 b ha bin, ha cutn, noe 66 hb bi ph thm mpL bp sp, 8 nhng eh 6 bn en id, ob tiny cht dng Pi rune vd ki, do 8 dung mB kh tim vo dre iu ‘Mang té bao co h8 b ha gb néa khong, b phi him lop oxy sie Mode caeicabonal, mang U6 bao rn day, in che, nn dung mbi kn thm vo dg iu. ‘Mang té bit co h8 i ph thm tp cht hy. ChB nhdy tan ge trong nude, nhung Kh it nude nb bl rong mB vb ted nbn nh, Lam bitin de ng mao qu rin ming 8 bao, gy cn WB sy thm ea dung ml nr qua ih eh tn, Do 6, v6inhcng rg fi 68g, rn che nén aay nhé docu, ao bk cho dung méi dB hm ws duge Hou. chit tan d8 Khe tan vo dung mi 2.42. Chit nguyén sinh Chit nun sinh 66 thnh phn isa nge phe ap va Kn én nh. Ch nguyn sin 6 tinh nh. nh nh, ng tan trong nude, Kg mau va khong bn dBi vr ni. nhit dd $0 60°C, ching bj mBt hoa tinh sina hoe (iy teuorg hyp nhimg hat kn, qua kb, chit nguyn sinh 6 thé chly due 4 80-4105°C). Cé thé ne ehdt nguyn sinh mgt mol tuéng di thé phic tgp, 6 hb oid la mth kao nhidu pha go thanh tr Ahong hop chit cas phn i, phn tn trong méi tng nude (id: it du, git mo, hat tinh be. alr th...) ‘19 26/08/2023 MOT SO KIEN THU'C CO BAN VE CHIET XUAT DUGC LIEU (tp) -Tin chuyén man & NOKH - Khoa Duge - Bai hge Duy Tan CChdt nguyen sinh inh ent ban thd, 6 nga a ch Bem abi Bi dung mBi ma kéng cho chBt tan qua. Do a6, ab chit dros ed cnt tan tong tbo, ng ta hal im cdch pha hiy chit rquyén sinh bing céch lam dng von caing bing nit (sy hod phot kno) Rage bang cin (ho neg rng. 24.3. Méts6 tgp chit céthb 66 trong duge lbu 16 I sin phdm cla cic qu nh tro och, a chdt err he cht tha cacy. Cc cht nay thug gay cn hoe cng cé thd te dyng thud I cho quan chidt xt. Dui dy a met sb tds ‘BS i nhing ae iu chia nhieu pectin, gém node cht ny: 16 1a nhing chit tan duce trong rue, va Khitan trong née th bj tung, go dung eich keo lam tang 4B abot gy cn 1 cho qua tenh chide mt thé loa céc cht ndy bing cach cho Ett rang bn cao 8, B51 ng arp iu chia nhieu tah Bt “Tesh b6t inh chit kéng tan trong nuselanh, nhung & mht a6 cao teh bgt bj hd néa, lim ang 46 nt cia cng eh 98y cén tr cho qua tinh chit xubt Do 46, di vei nhing duoc eu nay, Khdng nn xay dug Hou qué min, ténh oi héng ra nhibu th bot va khéng nn cid nit db cao 8 tr bi nS ha 'B4iyi hing are gu cia cht bo, edu ma, tinh du, sap, ahve 16 la nhing cht khong tan trong née va thing tan tong cdc dung mBlKhdng phn ove. Néu dng dung mB hit vb, ce cht ny 5 lam dung ma kn thm vio duge lu, gy cin tr qua tron cht wut, dod, cn ph lal lb cee dung mal tich hop tude Ki cid. Néu ding dung moi kxéng phan cye dé cht, ech cnt 8 lin niu tap, nhing tap nay 38 loa trong gi éaon tinh ond 186) nhdng are iu chia enzym Enzymn c bin cht 8 protein, nt 6 60-70°C, enzym bj mat hoat tinh, cn & mht tant enzym eb ngrng oat ‘ng, sau dB ndu nang abr rst B hien hyp thi enzym li drge phye HB. Tay ting wudna hyp ey thé mab enzyn 06h 98y edn 8 hoe eng 6 it du ig thug cho qua rnh eiét wut. VI dy: ayeosd tong ey bao gm hal ‘ninth rong va phn khng duong. Dub te dung cha enaym vin e6 sin wang duge NB, gp dibs Kg thu (im, fim), yosis Kd by phn (C6 3 phirong php dé dit enzym: + Phuong pip nt ust: ning due iu vio chit tng 85 (nue hod en) + Phuong php nit dn cho dug iu qua holm (ho nude s8inay hes ebn 8) ++ Phuong php nit kn: edo ge gu qua abng Hh8ng HY nbn 22, Nhimng yéuté thug v8 dung méi Mats yu beta dung m6 eds hg Lr qua inh ert xu fa 4 phn eve, 6 nhs, se eng BB mat 2.21. BB phan eye cia dung mei No chung dung mt phn ove thi dB héatan ci hd kg phn eye va kno ha tn cc chit cd nib nm phn _e. Navel, dung mBi phn ce man i hoa tan cde chit e6 rhb rh phn exe va kn hoa tan cc chit phan ove. Dua vito a phan ove oda dung mdi nqvdta phan loa ay sau “Dung mo khng phn ove ther du née, xéng,hexan,heptan, benzo olen, + Dung mi phan eve ybu va wi: eorofom, dlorolhan,aceton, + Dung mi phan eve manh: nude, gheern,cbn 2.22. DB nhot, ste cng bb mat cua dung mei [No chung, dung mel 8 66 nt eng thép hoc 8 sic cdng bi mat cdng nd th dng msi cing ‘go du Kg thud oi cho qua trnh chit sub va nguac a Mot sé 9 nhot a cde dung mel thudng 3p iim vio duge Hu 2.3. nhing ybut6 thu vd Ky thudt 1 1 nhing yéu tb hb thay i duce dng cic bib phip kj thugt tha nhau, rm tg ranhing ib kde thud ei cho qua trinn chit xt. D6 thé la nhing yu: nhs, th lan, 9 min cia ge Ku. khudy te, sium. 2.3.4. Nhlgt 9 chiét x ‘Teo céng tic tinh he sb uch tan cia Enstin, kn nit tg hi ng sb knuéch Ian cing tang, do 46, theo nh Bt Fick, lvong ch knudch tin cng tang ln. Hon na, khinnit 8 tang th 8 nhot ca dung mB gdm, dod 5 tg dtu én bud lo oe qua inh cht xudt tong met 9 rung hop eau ++ Bbivéinhing hep chit xém bn & nhit db cae: hit ang cao $8 a8y pha hy mo s6 hoat cht ahu vam, yeosid, acai ++ 61 v6i tap: kn nhig db tang, khdng cid tan ela hopt cht ang mad tan ca tp cng ding hoi tng theo, ch chit sb lin nn tap. Nn lal ves mots tap nh gm, ent nh. KD mht tang 8b rzng nt Bot bj hb ha, 49 mhet ci ch ent seb tang, gay kn Khan cho qua inh chit xu, tn ch. + bi voi dung moi dB bay ho cénhit 8 hp: KN tng nhit th ung mold bj hao yt. Kh tiem pl Kn a palo 86 phon Bi lvu dung ma + bi vi mot sb cnbt de bit c8 qua tinh Noa tan tba oh: Ki nit db tng, tan ea chung lig. Do 6, ab tanga tan th cn pi lam stim nhs 36 ‘+ Turnhang phan tic én, ta thy ty ting tng hep cu thé ma cbr ya chon rhBt a S90 cho pha hep (ty thuse vo eae yu 8 nn due Hou, dung mt, puna phap ent xu.) 23.2. Tho glan chide xudt Kr bt ds cid, ee cht 06 ph trong nbd ht 1 hogt eat) 96 due hd ton va khubch in vbo dung mdi tube, sa db mt én ce ch 0 pn kena lam (urn Wap ny nya, Keo. De 4, nut gian ent agin 38 khSng eis ge N6t hoa eh org due Bu; nhung nu the lan cit dai qu leh ent 9 b Hin Mh tap, oY bt tol ebo qua vin th ehé va bo qn. Tém a,

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