INTRO TO IMMUNOLOGY LAB [SERIAL NO: 4]

VIALS USE IN PHLEBOTOMY

(use of differently colored vials in phlebotomy)

These were evolved with the aid of Joseph Kleiner in 1947 and are currently being produced
with the aid of Becton, Dickinson and organization, a global issuer of clinical substances to the
healthcare and pharmaceutical enterprise .Extraordinary phlebotomy tubes are identified with
the aid of the color of the stopper. Each color is specified for different tests.

Dark Green colored vial :

Additive : sodium heparin

Phlebotomy vials with green top contain sodium herapin, lithium herapin and
ammonium herapin, that is actually coated in the inner of the tube. Tests :
 Insulin
 Renin and aldosterone
 Aluminium
 Gut hormones
 Amino acids
 Homocysteine
 Chromosomal tests.

Red Colored Vial :

Additive : silica particles

Vials with red-colored lids are used in serology and immunohematology. Serology is
done to check
 syphilis
 rubella titer
 strep testing
 pregnancy test
 cold agglutinins
 haptoglobin

Purple colored vial :

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INTRO TO IMMUNOLOGY LAB [SERIAL NO: 4]

Additive: EDTA

Vials with purple colored lid contain EDTA additive that binds with calcium ions,
effectively inhibiting coagulated Full blood count.
 Erythrocyte sedimentation rate
 Blood film for abnormal cells
 Reticulocytes
 HbA1C for diabetic control
 Parathyroid hormone.

Yellow colored vial :

Additive : sodium polyanethol sulfonate

These vials are used for a huge variety of

tests requiring separated serum for analysis
 Immunology tests
 Drug level tests
 Toxicology tests
 Tumour marker tests
 Endocrinology tests
 Biochemistry tests

Grey bottle vial :

Additive : Sodium Floride And Potassium Oxalate

As compared t the yellow one this vial is used cony for two purposes. It
basically used for biochemistry in which whole blood is required for analysis.
 Lipid panels
 Hepatic function tests
 Electrolytes
 Markers of cardiac damage
 Protein electrophoresis
 Evaluation of endocrine and metabolic disorders
 Therapeutic drug monitoring and toxicology

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