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Aspergillus Terreus As Lovastatin Producer To
Aspergillus Terreus As Lovastatin Producer To
Abstract. Ruminants produce methane emissions (CH4) which has contributed to the accumulation of greenhouse gases in the
atmosphere that have an impact on global warming. Methane produced from enteric fermentation and animal feces. Digestion in the
rumen process involves several components of microorganisms such as fungi, bacteria, and protozoa. Rumen microbial role in the
production of methane that methanogenic bacteria and protozoa which mostly live in symbiosis. Protozoa is an agent that serves as a
unifier between methanogenic with her in a symbiotic ciliate, causing the formation of methane. With the decline in the numbe r of
protozoa was possible mechanism symbiosis between ciliate protozoa and methanogenic disrupted resulting in methane formation is
not sufficient. Bacteria and protozoa body structure has the form of cell membrane phospholipids composed of charged phosphat e
group and attached to the two-chain fatty acids are associated with the presence of cholesterol. The most important stage in the
formation of cholesterol is the formation of mevalonate from HMG-CoA which occurs in the first stage. This process involves the
enzyme HMG-CoA reductase. HMG-CoA reductase is the target of HMG-CoA reductase inhibitors (statins) to inhibit cholesterol
synthesis. Statins inhibit the synthesis of mevalonate are spesific. Writing this idea aims to assess the effect of the addition of
inhibitors lovastatin from Aspergillus terreus to decrease methane production in cattle towards environmental friendly farm. Based on
the ability of lovastatin to inhibit the synthesis of cholesterol which is a component of the cell membranes of bacteria and protozoa, it
can be concluded that the compound lovastatin may be inhibiting agents ruminant methane production. Fungi from the
Deuteromycetes genus (Aspergillus sp. and Penicillium sp.) have been reported to potentially produces lovastatin, a secondary
metabolites as an anti-hypercholesterolemia agent (Chang and Miles, 1973). Feed fermentation using Aspergillus terreus can reduce
methane production.
B. DISSCUSSION
Ruminant digestive process performes occur in three
stages, mechanical digestion occurs in the mouth, enzymatic
digestion in the intestine, and fermentative digestion performed
by rumen microorganisms. Ruminants are polygastric animal
which digestive process not only by his own body, but also
fermentation process by microorganisms in the stomach (rumen)
[28]. Methane production by microorganisms in the rumen
consist of bacteria, fungi, and protozoa [12]. Methane production
by methanogenic bacteria decrease the gross energy produce
from the fermentation of feed about 6 to 10%. Methanogenic use
a hydrogen as a source of energy to form methane [14].
Protozoa also plays a role as predator of rumen bacteria
thus reduced the use efficiency of nitrogen in the rumen. These
could be a negative effect of protozoa existence to protein
metabolism in ruminants [27]. Protozoa perform a symbiosis
with methanogenic bacteria to produce a methane. Therefore, a
decrease the number of protozoa is the appropriate mechanism to
reduce methane production. Ciliated protozoa are known to have
interspesies hydrogen transfer mechanism specific to have a
symbiotic relationship with the endo and ecto methanogenic
bacteria. Methanogenic bacteria that bind to ciliated protozoa Fig. 1. cycle mevalonate formation and working principle of the
that mainly come from families Methanobacteriaceae [9]. enzyme HMG-CoA
The decreasing of methane is closely associated with the
presence of protozoa in the rumen. Symbiotic relationship Can be seen in the image above that the mevalonate
between methanogenic with ciliate protozoa shows how compounds produced by reducing HMG-CoA [15]. HMG-CoA
important a role in preventing the accumulation of methanogenic is a compound that is soluble in water and can be metabolized
hydrogen via hydrogen transfer mechanism interspecies with another when the enzyme HMG-CoA reductase is inhibited [30].
ciliate protozoa which causes only slight hydrogen converted HMG-CoA reductase is target of HMG-CoA reductase inhibitors
into methane [11]. (statins) to inhibit cholesterol synthesis. Statins inhibit the
Bacteria and protozoa body structure has the form of cell synthesis of mevalonate are specific [15].
membrane phospolipids (cholesterol) composed of charged Lovastatin can be produced from several species of fungi
phosphate group and attached to the two-chain fatty acids [20]. such as Aspergillus tereus, Aspergillus parasiticus, Aspergillus
Cholesterol formation occurs through four stages. The first stage flavus, Aspergillus umbrosus, penicillium funiculosum,
is the condensation of three acetate to form a six-carbon Trichoderma viridae, Trichoderma longibrachiatum,
intermediates and mevalonate. The second phase is change the Acremonium chrysogenum [23], Monascus pilorus [16],
mevalonate into active isoprene. The third stage occurs Aspergillus fischeri [22][13], Monascus purpureus [16],
polymerization of 5-carbon isoprene to form carbon 30 squalene. Aspergillus niger [22], and Aspergillus terreus [21].
The last stage is the cyclization of squalene to form four rings of References [24] shows that Aspergillus sp is one of a
the steroid nucleus through some reaction (oxidation, removal or group of fungi that have asexual spores Askomikota
transfer of a methyl group) to form cholesterol [17]. The most conidiophores found on the head. References [6] shows that
important stage in the formation of cholesterol is the formation Aspergillus terreus as one of the species of the fungus
of mevalonate from HMG-CoA which occurs in the first stage. Aspergillus sp can live optimally at temperatures between 35 and
This process involves the enzyme HMG-CoA reductase. HMG- 40oC and the maximum temperature for life is between 45 and
CoA is an integral membrane protein of the endoplasmic 48oC. References [6] shows that Aspergillus terreus conidia
reticulum smooth [19]. spores can germinate at a temperature of at least 11 to 13oC. In
Lovastatin as one of the statin compound will affect addition, this type of fungus can tolerate an environment that has
populations in the rumen microorganisms that can cause changes a high salt concentration, therefore, this fungus can be found in
in methane production. [15] Mevastatin or lovastatin did not brackish water marsh and coastal areas.
inhibit the growth of rumen bacteria specific ferment cellulose, Aspergillus terreus can be obtained by isolation using a
starch, and other polysaccharides into acetic acid, propionic, and dry grapes. References [25] shows that Aspergillus sp.
butyric. Here is a scheme involving the formation of mevalonate commercially used as a producer of organic acids and secondary
HMG-CoA. metabolic compounds such as lovastatin. References [10] shows
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