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The MFPI – Quality Control Laboratory is an advanced, accredited research facility of the University
delivering end to end food testing solutions of raw and processed food commodities. The lab takes up research
studies in determining various nutritional, bio-active, functional and anti nutritional composition in a wide
spectrum of food matrices existing in food value chain, using standard test protocols and high end equipment
maintained in state of calibration with internationally traceable Certified Reference Material. The lab is under
accreditation with NABL and implements ISO/IEC 17025:2005 Quality Management Systems.
The paradigm shift in Indian agriculture witnessed in case of food commodities from quantity to quality
is a welcome phenomenon in the context of present World order. With a total food grain production of 250 mt,
32 m MT of fruits and 71 m MT of vegetables India has established itself as food secured nation. The rapid
growth in allied sectors like diary, poultry and fisheries has further consolidated India's position as a nation with
marketable surplus. Consequently, our country has transitioned from production economy to market economy
encompassing growth, productivity, diversity, profitability and quality assurance. This transformation has led to
a spurt in processing activities of food commodities at various levels leading to free availability of processed
food at affordable cost. The pivotal role of “Testing Laboratories” in ensuring high quality standards of food
material has become much more relevant than before.
"Food safety" implies absence or acceptable and safe levels of contaminants, adulterants, naturally
occurring toxins or any other substance that may make food injurious to health on an acute or chronic basis.
Food quality can be considered as a complex characteristic of food that determines its value or acceptability to
consumers.
Food Quality Assurance is accomplished with the implementation of GAP, GMP, GHP, HACCP, MRA,
QM, ISO 9000 series (9000-9004) and 14000 and TQM. GMP covers the fundamental principles, procedures
and means needed to design an environment suitable for the production of acceptable quality. GHP describes the
basic hygienic measures that establishments should meet and which are the perquisites to other approaches, in
particular HACCP. GMP/GHP requirements have been developed by governments, codex and food industry in
collaboration with other groups, food inspection and control authorities.
In this context, main objective of the enforcement of quality assurance is to improve capabilities of a country
to enforce food safety norms according to Codex standards including food additives, food contaminants and heavy
metals, methods of analysis, sampling methods, import and export inspection and certification systems, residues of
veterinary drugs, pesticide residues, microbiological hazards, labeling, codes of practices, allergens, novel foods and
GMO's and product composition.
In India the “Food Safety and Standards Act of 2006” (FSSAI) came into force across the country on August
5, 2011, making it at par with the international standards. The act is enforced to ensure improved quality of food for
the consumers and censure misleading claims and advertisement by those in food business. FSSAI, established under
the overarching legislation, will lay down science based standards for food items and regulate their manufacture,
storage, distribution, sale and import to ensure availability of safe and wholesome food for human consumption.
Implementation and enforcement includes the areas like managing pesticide residue, traceability, testing facilities,
promote or penalise, penalty provisions, consumer safeguards, safeguards for food businesses and labelling.
To accomplish the task of Food quality control as per the provisions under FSSAI, an analytical
capacity is the basic requirement to monitor the safety of food. An efficient lab facility engaged in food quality
analysis needs to integrate analytical capability for microbiological safety of food, toxicity of chemicals in
foods, novel foods and processes, pesticides and veterinary residues.
MFPI-QUALITY CONTROL LABORATORY, PJTSAU, RAJENDRA NAGAR,
HYDERABAD
The Genesis
In pursuance with the vision for establishment of efficient Food Quality Control System, PJTSAU had
prioritized setting up of state-of-art, accredited Quality Control Facility to assist institutions and
entrepreneurs in fulfilling the requirements for implementation of international standards by analysis
of food samples, providing quality assurance services and technology transfer. Through an MOU
with the Ministry of Food Processing Industries, Government of India, the University in the year
2007-08 has established a well equipped Laboratory with high end analytical instruments run on
internationally accepted protocols. Essential infrastructural facilities include well furnished testing
lab rooms, a dedicated power line, soft water supply line, uninterrupted power supply to support all
the equipment, enclosure for gas cylinders and fire fighting equipment.
Objectives
Beneficiaries of deliverables:
University researchers, farmers, food processing sector, various national and international agricultural
research institutes (ICRISAT, ICAR, SAU’s, IICT etc) and consumers.
The MFPI-Quality Control Lab at Professor Jayashankar Telangana State Agricultural University is
committed to the standards of ethical conduct which define the obligations of public service. The QC lab
also has the responsibility of satisfying all the stake holders and be conscious of its environmental
responsibilities. The laboratory management and personnel are oriented towards performing quality
activities to assure integrity, accuracy, precision, reliability and timeliness of the data to be in the
service of farmers and the society at large. The laboratory's standard of service for the testing
programme is well defined and towards this objective, we will train our human resources on a
continual basis and keep them motivated to give optimum performance.
General requirements for the competence to carry out tests and/or calibrations as per
international standards specified under ISO/IEC 17025:2005(E) are complied with, in carrying out all
the pre analysis and analytical protocols of the Lab.
Performance results:
The present Quality Management System includes documentation pertaining
to the following quality control activities:
1. Equipment maintenance, calibrations, AMCs, intermediate checking
and trouble shooting.
2. Proficiency testing and inter-laboratory testing for proximate analysis
3. Training of Staff on quality and managerial aspects of ISO/IEC-
17025/2005, as well as equipment usage and handling of SOPs
4. Organization and personnel records
5. Document control to establish high degree of traceability
6. Continuous improvement through customer feedback and handling of
customer complaints
7. Utilization of NIST traceable reference standards for error free results
8. Adoption of stringent internal quality control mechanism for accurate
results
9. Quality plan, retesting and repeat testing for competence of testing
capability of staff
10. Maintenance of records pertaining to uncertainty measurements as a testimony of precision of
the results issued by the laboratory. NABL accreditation certificate
Impact & benefits:
Availability of an accredited laboratory working on the principles of ISO/IEC 17025:2005 quality
management system provides strong science based approach towards food related research. This will
enhance the credibility of the University in terms of data base generation and its utilization with regard
to crop genetic resources developed by the university and also add value to the processed products
designed out of those varieties through the efforts of food scientists of the laboratory. Such accredited
facility will help the stake holders of food chain in meeting the labeling requirements of FSSAI.
Location
The lab is situated on the Himayat Sagar main road in the Extension Education Institute campus of
PJTSAU, Rajendranagar, Hyderabad - 500 030.
Provisions :
The total operational area of the lab : 6000 s ft
2. Sr. Scientist 1
3 Research Associates 2
4. Technical Assistants 4
5. Computer Operator 1
6. Lab Attender 1
7. Security guards 1
ANALYTICAL CAPABILITY
I. Elemental estimation
Scope
Natural processes and contamination influence elemental levels in food material. Elements with
potential health hazards are Mercury (Hg), Palladium (Pd), Chromium, (Cr), Manganese (Mn),
Cobalt (Co), Nickel (Ni), Arsenic (As), and Cadmium (Cd) etc., Rapid industrialization and metal
prospecting has increased the levels of toxic metals in aquatic and soil ecosystem. Heavy metals
being immobile get accumulated in soil and food material. Such accumulation leads to the entry of
metals into food chain. On the other hand mineral profiling of food substances indicates the
nutritional worth and presence of essential elements which play a vital role in metabolism.
Estimation of trace elements upto ppb levels enhances the validity of the data generated in this
regard.
Execution
Gases used
Nitrogen, Argon
Sample range
Cereals, vegetables, pulses, fruits, processed food items and agricultural resources.
Clientale
Department of Agriculture, Agricultural research stations of PJTSAU, ICAR research
institutes like DSR, DRR, CRIDA, Private food industries, Farmers, Research Scholars, Food
and Pharma industry.
Gases used
Helium, Oxygen
Sample range
Cereals, Pulses & their Products leafy vegetables, vegetables, herbal preparation,
cooked products etc.,
· Model : 1029
· Make : Gerhardt
Technique
Estimates fat/oil content in the sample. The sample is extracted with petroleum ether. After
extraction for 1 hr. 36 min. the difference in the weights gives the fat content. Handles four
samples at once.
Sample range
Cereals, Pulses & their products, leafy vegetables, herbal
preparations etc.,
Fibertherm Crude Fibre Analyser
· Model : FT12
· Make : Gerhardt
Technique
Estimates crude fibre content in the sample. At a time digests 12 samples. Moisture and fat free
sample is digested for 1 hr. 46 min. and kept in the hot air oven and muffle furnace. The difference in
the crucible weight gives the weight of the fibre content in the sample per 100g.
Sample range
Cereals, Pulses & their products, leaf vegetable, vegetables, herbal preparations, cooked products etc.,
III VITAMIN AND AMINO ACID PROFILING
· Model : Polar10B00 · Model : 1260 Infinity
· Make : M/s Varian · Make : M/s Agilent
Technique
· Pumps : Quaternary pump module
· Column : C-18 Reverse phase column
· Mobile phase : Hexane sulfonic acid Na salt, Methanol, Aceto nitrile and
Chloroform
· Detector : UV –Vis detector, RI Detector, Fluorescence Detector
· Light sources : Deuterium lamp for UV and Quartz halogen for Vis
Estimates Amino Acid composition and B complex vitamins(thiamin, riboflavin, pyridoxine, niacin,
Nicotinamide, Folic acid) fat soluble vitamins (Vitamin A and B carotene), gossypol and isoflavones,
etc., using NIST traceable standards at one run for 20- 30 min. Samples are extracted with suitable
solvents and injected into HPLC. Quantification is done based on the standard retention time and
peak area.
Sample range
Cereals, pulses and their products.
UV-VIS Spectrophotometer
· Model : CE 7200
· Make : CECIL
Technique
Dual silicon photodiode
WL Range : 190-900 nm
WL calibration : Automatic at switch on
Works on the principle of light absorbance. Standards are prepared at different concentrations
and the colour is developed with different intensities. Standard graph is prepared based on the
absorbance. Once the samples are processed the concentration of the sample for the particular
parameter is read in the standard graph. Estimates sugars, carbohydrates, phytates , tannins,
total and B carotene and protein at different wavelengths.
Sample range
Cereals, Pulses, leafy vegetables, other vegetables and their products.
Clientale
Agricultural research stations of PJTSAU, ICAR institutes like DSR, DRR, CRIDA, APEDA,
Private food industries, Farmers, Research Scholars, AP Foods and Pharma industry.
III. FATTY ACID PROFILING
Scope
Edible oil consumption constitutes major portion of dietary intake by average Indians. A
wide variety of refined sources of edible oils are available in modern society. These sources from
different oil seed crops vary in the constitution of fatty acids making them specific for this
purpose. Though unsaturated fatty acids are recommended for good health, excess unsaturation
also results in poor keeping quality of oils. Hence, it is very essential to have a balance between
saturated and unsaturated fats to avail benefits of good health and consumer quality. In this
connection, profiling of major fatty acids available in oil seed crop varieties released by PJTSAU
is essential so as to prepare a data base for use by the Breeders and others concerned for
developing elite varieties in future, and also identify any unusual changes in composition which
might have beneficial/detrimental effects on health.
Execution
Equipment
Gas chromatography
Technique
Detectors
· FID ( Flame Ionization Detector)
· NPD (Nitrogen phosphorus Detector to detect Organophosphate pesticides)
· ECD (Electron capture detector to detect Organochlorine and Carbamate pesticides)
· Solvents : Acetone, Acetonitrile, Dichloromethane and Hexane.
· Capillary Column (5% Diphenyl, 95% Dimethyl polysiloxane)
· Carrier gas : Nitrogen
· Equipped with Autosampler
Standards available : Acephate, Carbofuran, Chlorpyriphos, Chlorpyriphos-methyl,
Dimethoate, Endosulfan, Malathion, Monocrotophos,
Quinolphos.
Sample is extracted with suitable solvents and then injected into GC using FAME procedure. Sample
gets vapourized and migrates along with the mobile phase i.e, carrier gas and is then detected.
Pesticide in a sample is quantified by plotting against standard retention time and peak area.
Gases used:
Nitrogen, Hydrogen and Dry air.
V. MICROBIAL TESTING
Scope
Presence of harmful microbial organisms in food stuffs is a major emerging challenge
worldwide, to comply with Food Safety regulations. Toxins released by microbes are potentially
hazardous. Testing of food samples for the presence of bacteria, fungi etc., warrants establishing
lab infrastructure with international standards.
Technique
Clean rooms with modular wall panels and non walkable ceiling panels supported by
recirculation Air Handling Units comprising of Heating Ventilation Air Conditioning system
(HVAC) and High Efficiency Particulate Air filters (HEPA) of 99.997% down to 0.3 micron rating
are under operation. The entire clean room area of approximately 600 sft comprises of three
change rooms, sample preparation/ instrumentation room, clean corridor, three clean rooms and
a critical area housing the Laminar air flow. The entire facility is validated for parameters like air
balancing, room pressure adjustments, temperature and relative humidity adjustments, terminal
HEPA installation leakage test, clean room classification test and documentation of validation
and testing results as per ISO-7 and ISO-8 standards.
Equipment
Laminar Air Flow Chamber (LAF)
· Make : Smart lab tech Pvt.lt
Used for sample inoculation and serial dilution, sub culturing, culture inoculation. LAF bench is
kept in restricted area to maintain the sterile condition.
Provisions
· HEPA FILTERS : To check the performance of Air Quality system
· MAGNAHELIC GUAGE : To check the pressure flow inside the chamber using Valometer
(12 to 18mm)
· FLAME : Glass beads or electric burner.
· PASS BOXES : To pass the autoclave material to LAF bench.
· HYGROMETER : To check the room humidity
Sample range
Cereals, flours, fruit juices, beverages, rural and urban cooked and raw food items, organically
developed antifungal agents, medicinal plant leaf powders, water samples.
Clientale
Food and beverage industries, UNICEF projects, Research scholars, Faculty of Home Science.
Radiation processing of Seed, raw grain and processed food commodities can be irradiated by exposing
to Gamma radiation at desired dose rate. This could be applied for purposes like enhancing shelf life, to
induce point mutations, surface disinfestations and disinfection, to maintain seed viability of field crops
etc.,
SAMPLES ANALYZED SO FAR: (2007-2018)
Toxins 10
Microbiology 1951
Irradiation 1490
Vitamins 1650
Proximates 8228
TOTAL: 33,655
Scope of Analysis Testing fee (in Rupees) per sample
(Name of analytes & matrix) Other organizations / PJTSAU Farming
agencies community
Commercial Research Research schemes / (limited to few
testing purposes colleges samples)
1. Foods & Agricultural Industry / Other PJTSAU PJTSAU Farmers
products institute students Students