Primary and Secondary

Screening
Crowded Plate Technique
Members:
Mearelle Kate Alfante, Sigred Evangelista
Glenn Royce Alba, Regie Japsay
Types of Screening
Primary screening helps to
determine the microorganisms
and organic acid in cells and
other desired organisms.

Secondary screening can be

used for determining the
quantitative and qualitative
information about the cells,
desired organisms or strains.
 Crowded Plate Techniques

This technique of primary screening helps

to determine the microorganisms that are
capable of producing the antibiotics or
not.

A “suitable aliquot” of “serial dilution” is

chosen that can produce 300 to 400
colonies when it is plated on the agar
plate, which is also called a crowded
plate.
Source:Screening of industrially important microorganism - industrial microbiology.
BrainKart. (n.d.). Retrieved October 27, 2022, from
https://www.brainkart.com/article/Screening-of-Industrially-Important-
Microorganism_40998/
 PROS CONS

Still requires further

Simple, fairly rapid
analysis

it will not give indication of

good technique for
antibiotic producing
obtaining microorganisms
organism against a desired
that can produce antibiotics
organism
 Application of the Technique
Split-agar assay of antifungal soil microbial metabolites
by Aparna & Rao

Aparna, K., & Rao, D.L.N. (2016). Split-agar assay of antifungal soil microbial metabolites. Biocatalysis and Agricultural Biotechnology, 6, pp 184-188.
Highlights of the Research
Development of a novel method of quantifying the soil
suppressiveness of phytopathogenic fungi
Modified crowded plate technique was employed in obtaining
the antagonistic microorganisms
Thank you!