A.S.

Shelten George
E-mail: g_shelten@yahoo.com

Objective

Being a post graduate in Bio-Physics (Life Science) I am looking for an Institution

where I can actually utilise my experience and knowledge. I am willing to contribute my
knowledge that I have achieved in the former years of my academic studies to help an
Institution in fields of Life Science.

Education

 M.Sc. Bio-Physics from School of Biosciences, Mahatma Gandhi University, Kottayam,

India) in 2010.
 B.Sc. Industrial Microbiology from National College of Arts and Science, (affiliated to
the University of Kerala, Trivandrum, India) in 2007.

Knowledge Purview

Biotechnology

 Basic techniques in Microbiology, Cell biology and Immunology

 Basic Biochemical techniques

Molecular Biology

 DNA isolation
 Electrophoresis(AGE,PAGE)
 PCR techniques
 RFLP
 RAPD
 Western blotting
 Chromatography
 ELISA techniques
 Gene Cloning Vectors

Bioinformatics
 Genomics
 Proteomics
 Phylogenetic analysis
 Micro arrays
 Computer Aided Drug Designing
 Molecular Modelling
 DNA Sequencing
 DNA foot printing
 Basic Bioinformatics software’s handling
 Databases: NCBI, GenBank, EMBL, DDBJ, Swiss-prot, PIR, PDB, PRODOM, PRINTS

IT Skills

 Operating systems: MS-DOS, Windows2007, XP, Linux, Ubuntu

 Microsoft Office(Word, Excel, PowerPoint)
 Programming Languages: C, Basics of Java

Soft Skills

 Hard working
 Quick learner
 Self-motivated
 Communicator

Projects

 Done a Project Work, “PRODUCTION OF Calocybe indica- MILKY MUSHROOM” at the

College of Agriculture, Thiruvananthapuram as part of the B.Sc Industrial Microbiology
Course.

 Done a Project Work, “SYNTHESIS AND ACTIVITY STUDIES OF DE-NOVO DESIGNED

ANTIMICROBIAL PEPTIDES” at The Rajiv Gandhi Centre for Biotechnology,
Thiruvananthapuram as part of the M.Sc Biophysics Course.

“SYNTHESIS AND ACTIVITY STUDIES OF DE-NOVO DESIGNED ANTIMICROBIAL PEPTIDES”

Synthetic peptides and their derivatives which can act as antibiotics or cell penetrating
molecule is of great importance. A fundamental understanding of the influence of their
structure, nature of amino acid, sequence on activity and mechanism will be an important
part of any future strategy aimed at solving the growing problem of microbial resistance to
currently available antibiotics or delivering molecules in specific sites inside the cell without
causing cytotoxicity. Our research also involves the design and synthesis of a biodegradable
polymer of nano size as drug delivery system and developing new strategy for the chemical
synthesis of mini-proteins. Though the foundation of all these areas of research is organic
synthesis, chemical biology enables us to tackle these scientific problems on the threshold
between chemistry and biology.

The growing problem of resistance against existing antibiotics, coupled with a sustained
decline in the success rate of the discovery of new ones, is currently leading to a point in the
future where many infections could essentially be untreatable by the compounds that are
currently available. Amphibians and insects have enjoyed remarkable evolutionary success
and emerged as the most successful clade of all organisms constituting close to 80% of all
animal life forms. Living and evolving closely with other dominant life forms such as
microbes, they have developed an amazingly strong resistance to infectious microbes and in
many cases established symbiotic associations with microbes. Peptides which can kill broad
spectrum of microbes are widely distributed throughout the animal and plant kingdoms is
the defensive weapon that give them protection and served a fundamental role in the
successful evolution of complex multicellular organisms. These molecules could act as the
possible candidate molecules against microbial infection.

The main objectives of this project are;

 To identify the functional elements from non-coding DNA

 To predict the tertiary structure
 To estimate the stability of the protein structure

This study intended to identify novel functional peptides from non-coding region of
Saccharomyces cerevisiae.1561 intergenic sequences of Saccharomyces cerevisiae which
share no considerable similarity with the available sequences in the NCBI are taken for study.
Out of the 1561 sequences, 433 sequences showed ORFs which are used for functional
analysis, tertiary structure prediction and determining the stability of the protein structures
by calculating various parameters including hydrogen bonds, free energy, stabilization
centers, disulphide bridges, hydrophobic interaction, Ionic interaction and cationic-pi
interactions. The antimicrobial activity of the functionally predicted sequences was also
determined.

Bioinformatics software’s and tools used,

1. Databases: SGD, NCBI

2. Alignment Tool: BLAST

3. Software used to predict ORF: ORF FINDER

4. Subcellular localization prediction tool: WOLFPSORT

5. Software’s used for Function prediction: PROTFUN, PROTPARAM, SCANPROSITE,

INTERPROSCAN

6. Software used for Antimicrobial prediction: APD

7. Software used for Structure prediction: I-TASSER,SAVEs

8. Molecular Visualization Software: Swiss Pdb- Viewer

9. Stability prediction software’s: SCIDE,CAPTURE,PIC WEB SERVER, WHAT IF

Personal Information

Date of Birth : 23rd December 1984

Nationality : Indian
Sex : Male
Marital Status : Married
Languages known : Hindi, English, Malayalam
Permanent Address : Sathyanikethanam, Poonkulam, Near AGC., Vellayani.P.O.,
Trivandrum-695522, Kerala, India
Contact Number : +91-8590417772 (India)

Experiences

Completed One Year Training Course in Noorul Islam Institute Of Medical Sciences
(Bio-Medical Engineering Department), Neyyattinkara, Thiruvananthapuram.

Completed six months Training in Rajiv Gandhi Centre for Biotechnology (RGCB),
from May 2015 to October 2015.

Award

Terumo Penpol First Place in Blood Donation Quiz Competition.

Declaration

I hereby declare that the above mentioned information are true and correct to the
best of my knowledge and belief.

A.S.Shelten George

Sign

21/09/2021