NAME: ABORDO, ABSALUD, AGUILLAS, APULI, ARREOLA, BARROGA GROUP NO.

CARBOHYDRATE SAMPLE

Sample source Potato Starch

Characteristics White, thick, slightly soluble in water, precipitate settles at the bottom of
the container

Photo of isolate

PRELIMINARY QUALITATIVE DATA

Test Performed Result Interpretation/Inference

Molisch’s Test Purple ring at interphase It shows positive result.

Iodine Test Blue-Black Color It shows positive result.

HYDROLYSATES

Acid Hydrolysis Enzyme Hydrolysis

Weight/Volume of
Provided by facilitator 10g
Hydrolysate

Characteristics Viscous, white turbid Slightly soluble in water, precipitate settles at

liquid the bottom of the container

UNIVERSITY OF SANTO TOMAS Pharmaceutical Biochemistry Laboratory

FACULTY OF PHARMACY PHA 6112
Department of Pharmacy A.Y. 2022-2023
 QUALITATIVE DATA
Test Results
Performed

Acid Enzyme Standard

Standard Standard Standard Standard
Intact Hydroly Hydrolys (Fructose Standard Standard
(Glucose) (Xylose) (Lactose) (Sucrose)
sate ate )

Benedict N/A + - Blue w/ a Blue Dark Blue Blue w/ a Blue

’s Test Blue Dark blue Brick Red Brick Red faint of faint of
with a with Red Red
faint of black ppt.
red ppt.

Barfoed’ N/A - - - Blue with Blue w/ a Clear Blue Blue Blue

s Test Clear Blue Blue with a faint of faint of Blue
light a faint of red Red
blue red

Seliwan N/A + - Cherry Clear light Clear light Cherry Yellow Yellow
off’s Test Light Clear Light Pink red orange orange Red
pink light condensa
yellow tion
with
black ppt.

Bial’s N/A - - Light Clear Clear Clear Yellow Clear

Orcinol Bright Clear Light Yellow Light Light Yellow Yellow
Test yellow light Yellow Yellow Yellow
yellow
with
black ppt.

Mucic N/A - - - - - + - - -
Acid No No No No No Formed No No No
Test Crystal Crystal Crystal Crystal Crystal Crystals Crystal Crystal Crystal
(13 min Formati Formatio Formation Formation Formation (Shattered
Formation Formation Formation
Glass
& 6 s) on n Appearance)

Phenyl N/A + + + + + - + + -
hydraz 4.44 5 4.31 2 (Partial) Fine-Ion g Cotton-Ba Broad No
one Minutes Minutes Minutes Minutes 18 needle ll Shape Needle-lik Crystal
Test Needle-sh Needle-S Minutes shaped Crystal e crystal Formation
aped haped Fine-Ion g crystal Formation formation
crystal Crystal needle formation
formation Formation shaped
crystal
formation

THIN LAYER CHROMATOGRAPHY

UNIVERSITY OF SANTO TOMAS Pharmaceutical Biochemistry Laboratory

FACULTY OF PHARMACY PHA 6112
Department of Pharmacy A.Y. 2022-2023
 Illustrate the Computation of the Rf Value
chromatogra
m

CANNOT BE DETERMINED

Number of
spots from CANNOT BE DETERMINED
the protein
hydrolysat
e revealed
in the
chromatog
ram

Distances
travelled by the CANNOT BE DETERMINED
protein
hydrolysates
(spot) based on
the
chromatogram
(indicate for each
of the spots)

Distance travelled CANNOT BE DETERMINED

by the solvent

Rf values of the of CANNOT BE DETERMINED

the protein
hydrolysate

QUANTITATIVE DATA

Standard Sample

Concentrations Absorbance

T1 T2 T3 AVE

UNIVERSITY OF SANTO TOMAS Pharmaceutical Biochemistry Laboratory

FACULTY OF PHARMACY PHA 6112
Department of Pharmacy A.Y. 2022-2023
 2000 ug/mL 3.29 3.29 3.29 3.29

1000 ug/mL 1.669 1.76 1.757 1.729

500 ug/mL 1.705 0.698 0.721 1.041

250 ug/mL 0.466 0.469 0.793 1.728

125 ug/mL 0.227 0.238 0.238 0.703

62.5 ug/mL 0.7 0.067 0.76 0.71

Distilled water -

r-value 0.996127

Slope of the line 0.0015705

y-intercept 0.164

Acid Hydrolysate

Concentration 500 µ𝐿

Absorbance T1 T2 T3 AVE

3.047 3.047 3.047 3.047

Amount of Glucose

Y = mx + b
3.047 = 0.0015705(x) + 0.164
3.047 - 0.164= 0.0015705(x)
2.883 0.0015705(𝑥)
0.0015705
= 0.0015705 = 1835.721108 mcg

Enzyme Hydrolysate

Concentration 500 µ𝐿

Absorbance T1 T2 T3 AVE

0.246 0.246 0.246 0.246

Amount of Glucose

0.246 = 0.0015705(x) + 0.164

0.246 - 0.164= 0.0015705(x)
0.082 0.0015705(𝑥)
0.0015705
= 0.0015705 = 52.21267112 mcg

UNIVERSITY OF SANTO TOMAS Pharmaceutical Biochemistry Laboratory

FACULTY OF PHARMACY PHA 6112
Department of Pharmacy A.Y. 2022-2023
SIGNED DATA SHEET:

UNIVERSITY OF SANTO TOMAS Pharmaceutical Biochemistry Laboratory

FACULTY OF PHARMACY PHA 6112
Department of Pharmacy A.Y. 2022-2023