Home › Technical Reference Library › Protocols › Cell Analysis Protocols › NucRed Live 647 ReadyProbes Reagent Protocol

NucRed Live 647 ReadyProbes Reagent Protocol

Nuclear stain for live cells

NucRed Live 647 ReadyProbes Reagent is a bright far-red cell-permeant nuclear stain for live or fixed
cells. 

This protocol can be used for: You will need the following for this protocol:

Nuclear staining to identify nuclear condensation in apoptotic cells using fluorescence Cells growing in culture
microscopy NucRed Live 647 ReadyProbes Reagent (Cat. No. R37106)

Fluorescence microscope with far-red filter

This protocol should not be used for:

Flow cytometry

Protocol  
1. Culture cells in an appropriate medium and vessel for fluorescence microscopy. Protocol tips
In some cases, more or fewer drops may be
needed to achieve optimal staining
2. Add two drops of NucRed Live 647 Reagent per milliliter of medium. intensity.

Image quality may be improved by

replacing the culture medium with Live Cell

3. Incubate for 15–30 minutes.  Imaging solution.

4. Image the cells.

Spectral information and storage

  NucRed

Excitation/Emission (nm) 642/661

Standard filter set Cy®5

Nuclear staining in live hUVECs. hUVECs were grown

EVOS Light Cube Cy®5
overnight, stained with NucRed Live 647 ReadyProbes

Storage conditions Room temperature Reagent for 20 minutes at 37°C, and then imaged.
Ordering information

N.° de Catálogo R37106

Nombre NucRed™ Live 647 ReadyProbes™ Reagent

Tamaño 1 kit

Precio (EUR) 281,00

Cantidad

Añadir a la cesta