Introduction to Analytical

Chemistry

University of San Agustin

Department of Chemistry and Physics
Analytical Chemistry

is a measurement science consisting of a set of

powerful ideas and methods that are useful in
all fields of science, engineering, and
medicine.

Two Major Divisions:

1. Qualitative Analytical Chemistry
“chemical identity/ies in the sample”

2. Quantitative Analytical Chemistry

“relative amounts of ….”
Qualitative analysis is what.
Quantitative analysis is how much.

©Gary Christian, Analytical Chemistry, 6th Ed. (Wiley)

Forum Discussion
• Cite specific examples of relating/applying
analytical chemistry to other branches of
chemistry and other sciences
.
An analysis involves several
steps and operations which
depend on:
•the particular problem
• your expertise
• the apparatus or
equipment available.
The analyst should be
involved in every step.

©Gary Christian,
Analytical Chemistry,
6th Ed. (Wiley)

Fig. 1.1. Steps in an analysis

 Different methods provide a range of precision,
sensitivity, selectivity, and speed capabilities.

©Gary Christian,
Analytical Chemistry,
6th Ed. (Wiley)
 Calibration curves are prepared from standards.

Unknown concentrations are then determined

from the signal intensity of the sample.

Figure 1.2 Calibration curves for the measurement of proteins

using matrix-assisted laser desorption ionization (MALDI) – mass
spectrometry and anionic liquid matrix. (Courtesy of Prof. Michael
Gross, Washington University in St. Louis.)
©Gary Christian, Analytical Chemistry,
6th Ed. (Wiley)
Example 1: Chemical Analysis
 HPLC
-instrument used for the separation and quantification of
compounds
 Example 2: Chemical Analysis
Quantification
of Caffeine in
Selected Energy
Drinks
-Carisma, M., Loking
D., Saludes, J.
(Faculty Research)

General flow of preparation and analysis of samples.