Slip # : 230300990

Pat.Name : BAKHTO MAI Slip Date : 13/03/2023 10:42PM

Age/Gender : 55 Year(s) / Female Reported : 15/03/2023 12:30AM

CNIC No. : Ph.No. : 0 Registered : British Lab Nishtar Road

Specimen : Blood Performed : British Lab

Referred By : CITY LAB ANDDAIGNOSTIC CENTER

PCR HCV RNA (QUANTITATIVE)

Results: This sample is DETECTED for HCV RNA

Concentration: of HCV RNA is 7761 IU/mL

SENSITIVITY:
The test has been shown to be potent to detect at least 30 IU/ml using a HCV positive serum.

Principle:

HCV-diagnosis-by-PCR-is-based-on-the-amplification-of-specific-regions-of-pathogen-genome.-In-Real-Time-PCR-(RT-
PCR)-the-amplified-product-is-detected-via-the-fluorescent-dyes,-which-specifically-binds-to-the-5`-oligonucleotides.
An-Internal-Control-(IC)-is-run-with-each-sample.-The-HCV-and-IC-specific-probes-are-each-labelled-with-different-fluor
ophore,-thus-allowing-for-simultaneous-detection-of-both-amplified-product-at-each-cycle.-The-non-competitive-Internal-
Control-(IC)-is-detected-at-all-HCV-levels.-In-this-way-inhibition-in-the-sample-could-be-observed.-The-homogenous-for
mat-and-sealed-PCR-tubes-eliminate-chances-of-contamination-by-amplified-products.
To-obtain-results-in-copies/ml,-multiply-IU/ml-value-with-1.21.

Formula:-Results-in-IU/ml-`x-1.21-=-copies/ml.
RELIABILITY:
Due to the co-purification and amplification of synthetic internal control RNA maximum reliability of the HCV
quantification kit is granted the absence of PCR inhibitor as well as the correct performance of the extraction can be
detected. Using ready to use virus comp controls a confident quantification of HCV in human serum or plasma samples
is accomplished.

NOTE:
The titer of circulation HCV in blood fluctuates in accordance with the virus latency & variable results may occur in
different phase of viral infection. Therefore clinical correlation is very important Lab to Lab variation may occur due to
difference in sensitivity, specificity and reproducibility of different assay technique used .

Report Electronically Verified By : Unaiza Asim

Electronically verified report, not require any signature.

DR. SHAHID MEHMOOD DR.FAIZA SHAFQAT SADAF SHAHEEN

MBBS (Pb) MBBS, MPhil (Hemat) (M.Phil) Microbiology
Managing Director Consultant Pathologist PHD Microbiology