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Bio-Rad Laboratories IMMUNOHEMATOLOGY

ID-Centrifuge 6S / 6S Accu
ID-Centrifuge 12SII
ID-Centrifuge 24S
User Manual 13.0-01/2019

The Complete Solution for Safe Transfusion


Document

ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual

Version: 13.0

Document ............................................................H009120
ID-Centrifuge 6S / 6S Accu...............................009560 / 009563
ID-Centrifuge 12SII ............................................009550
ID-Centrifuge 24S ..............................................009530

Manufacturer

DiaMed GmbH
Pra Rond 23
1785 Cressier FR
Switzerland

Tel. +41 (0)26 67 45 111


Fax +41 (0)26 67 45 145

Version Date Comment

8.0 11.03 Eighth edition

9.0 27.01.05 Ninth edition, adaptation of the new layout

10.0 23.05.05 Reordering of the chapters.

10.1 30.08.05 Addition of ID-Centrifuge 6S Accu (section 5.2)

10.2 15.12.06 New document numbers WEEE compliance

11.0 25.10.10 Brand Change

12.0 01.09.18 Update of the Maintenance chapter

13.0 01.01.19 Update of the general safety instructions

Copyright ©

The reproduction, even partially, of this document is forbidden. No part may be copied in any form,
and it may not be used, edited nor transmitted by any electronic means (photocopy, photography,
magnetic supports or other recording processes), without the written authorization of Bio-Rad. All rights
and particularly reproduction, translation, edition, distribution and also industrial property and recording
are reserved.

2 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Table of Contents

1 Generalities......................................................................................................... 7
1.1 Appropriate Use ...........................................................................................................7
1.2 Glossary ........................................................................................................................7
1.2.1 Persons ............................................................................................................7
1.2.2 Product ............................................................................................................8
1.3 Typographical conventions ...........................................................................................9
1.3.1 Description .......................................................................................................9
1.3.2 Command ........................................................................................................9
1.3.3 Procedure.........................................................................................................9
1.3.4 Cross reference ...............................................................................................9
1.3.5 List of items ..................................................................................................10
1.3.6 Troubleshooting..............................................................................................10
1.3.7 Warnings ........................................................................................................10
1.3.8 Recommendation and Note ..........................................................................12

2 Safety and handling........................................................................................... 13


2.1 Introduction .................................................................................................................13
2.1.1 Principle..........................................................................................................13
2.1.2 Importance of the safety instructions ...........................................................13
2.1.3 Non-compliance with the safety rules ..........................................................13
2.2 Environmental conditions ............................................................................................14
2.3 General safety instructions .........................................................................................15
2.3.1 Observations and information........................................................................17
2.4 Special safety instructions..........................................................................................17
2.5 Storage conditions......................................................................................................17
2.5.1 Deactivation and storage...............................................................................17
2.5.2 Handling and transportation ..........................................................................18

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3 Description ........................................................................................................19
3.1 Principles of the process........................................................................................... 19
3.1.1 Basic principles ............................................................................................. 19
3.1.2 ID-Cards used ............................................................................................... 20
3.2 General description .................................................................................................... 20
3.2.1 Overview and designation of the main parts .............................................. 20
3.3 System description..................................................................................................... 21
3.3.1 Power socket, equipment fuse, and power switch..................................... 21
3.3.2 6S / 6S Accu control panel......................................................................... 21
3.3.3 12SII and 24S control panels ...................................................................... 22
3.3.4 Acoustic signals ............................................................................................ 22
3.4 Features...................................................................................................................... 23
3.4.1 Function description ...................................................................................... 23
3.5 Technical data............................................................................................................ 24
3.5.1 Performance data.......................................................................................... 24
3.5.2 Transport data ............................................................................................. 24
3.5.3 Physical data ................................................................................................. 24
3.5.4 Dimensions and weights ............................................................................... 25
3.5.5 Environmental conditions............................................................................... 25
3.5.6 Emissions....................................................................................................... 25
3.6 Commercial information ............................................................................................. 26

4 Commissioning ..................................................................................................27
4.1 Unpacking and setting up the ID-Centrifuge............................................................ 27
4.1.1 Installation site ............................................................................................... 27
4.1.2 Unpacking...................................................................................................... 29
4.1.3 Connecting the ID-Centrifuge to the power network .................................. 30
4.1.4 Unpacking the centrifuge head .................................................................... 30
4.2 Initial operation ........................................................................................................... 32
4.2.1 Configuration.................................................................................................. 32
4.2.2 Configuration procedure ................................................................................ 33
4.3 Faults during the initial operation .............................................................................. 36
4.3.1 Rectifying faults ............................................................................................. 36
4.3.2 Keeping a maintenance record .................................................................... 36

4 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
5 Operation .......................................................................................................... 37
5.1 Centrifuge 6S..............................................................................................................38
5.1.1 Normal procedures ........................................................................................38
5.1.2 Emergency stop.............................................................................................40
5.1.3 Other abnormal procedures ..........................................................................40
5.2 Centrifuge 6S Accu....................................................................................................41
5.2.1 Normal operations .........................................................................................41
5.2.2 Emergency stop.............................................................................................42
5.2.3 Other abnormal procedures ..........................................................................42
5.3 Centrifuge 12SII ..........................................................................................................43
5.3.1 Normal procedures ........................................................................................43
5.3.2 Emergency stop.............................................................................................45
5.3.3 Other abnormal procedures ..........................................................................46
5.4 Centrifuge 24S............................................................................................................47
5.4.1 Normal procedures ........................................................................................47
5.4.2 Emergency stop.............................................................................................48
5.4.3 Other abnormal procedures ..........................................................................49
5.5 Turning off the centrifuge...........................................................................................50

6 Maintenance ..................................................................................................... 51
6.1 Maintenance report.....................................................................................................51
6.2 Maintenance plan .......................................................................................................52
6.3 Auxiliary materials .......................................................................................................53
6.3.1 Cleaning agents .............................................................................................53
6.3.2 Measuring devices .........................................................................................53
6.4 Cleaning ......................................................................................................................54
6.5 Decontamination .........................................................................................................55
6.6 Check and test of the instrument.............................................................................56
6.6.1 Seal and lid ...................................................................................................56
6.6.2 Mobility of the ID-Cards holders...................................................................56
6.6.3 Checking the target speed ...........................................................................57
6.6.4 Checking the centrifugation time ..................................................................59
6.6.5 12SII Centrifuge: Inspecting the O-rings.......................................................62
6.6.6 12SII and 24S Centrifuges: Fan Filter ..........................................................62

7 Troubleshooting ................................................................................................ 63
7.1 List of possible problems ..........................................................................................63
7.1.1 Centrifuge 6S / 6S Accu ..............................................................................64
7.1.2 Centrifuges 12SII and 24S............................................................................66
7.2 Other operations.........................................................................................................68
7.2.1 Opening the lid mechanically ........................................................................68

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8 Appendix............................................................................................................69
8.1 Graphical symbols...................................................................................................... 69
8.1.1 Instrument-related symbols ........................................................................... 69
8.1.2 Package-related symbols .............................................................................. 69
8.2 Quality system............................................................................................................ 70
8.2.1 Decontamination certificate ........................................................................... 70
8.2.2 Maintenance record....................................................................................... 72
8.2.3 Performance variation.................................................................................... 74
8.3  Conformity........................................................................................................... 74
8.4 Device disposal .......................................................................................................... 75
8.4.1 General warnings........................................................................................... 75
8.4.2 Disposal ......................................................................................................... 75
8.5 List of documents...................................................................................................... 75
8.5.1 User Manual .................................................................................................. 75
8.5.2 Service Manual (english only) ....................................................................... 76
8.6 Identification plate ...................................................................................................... 76

6 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Generalities 1

Chapter overview

This chapter contains basic information on the structure of the document, its specifications and on the
documentation.

Preface

This manual is intended for all persons operating or carrying out any activities on the ID-Centrifuge 6S
/ 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S.

It contains all the information necessary for safe operation and work with your ID-Centrifuge.

The centrifuges are normally supplied with a User Manual available in several languages.

The Service Manual is available in English for the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or
ID-Centrifuge 24S but only to authorized technical staff.

 See section “8.5 List of documents” on page 75.


This manual is produced by DiaMed GmbH with the same care as the ID-Centrifuge 6S / 6S Accu,
ID-Centrifuge 12SII, or ID-Centrifuge 24S. It is a fixed part of the ID-Centrifuge. If you have any
questions or you are unclear about anything, please contact your Bio-Rad agent immediately or
contact us directly.

1.1 Appropriate Use


The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S may be used only for
centrifugation with ID-Cards.

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S may only be used by
trained and authorized personnel in a medical laboratory. It may not be used in the patient’s direct
environment.

The centrifugation of materials other than those described in the User Manual is forbidden (e.g. tubes
with sample material). Any other application is considered improper use of the centrifuge.

The instructions contained in the present User Manual must be adhered to, in particular the safety
instructions.

1.2 Glossary
The following terms, among others, are used in the present User Manual.

1.2.1 Persons

Manufacturer

The manufacturer of the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S is:

DiaMed GmbH, CH-1785 Cressier sur Morat.

Operator

The operator is the owner of an ID-Centrifuge, both when using it as its owner and when transferring
it to a third-party.

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Generalities

Personnel

The personnel gathers persons who have any kind of activity with the ID-Centrifuge 6S / 6S Accu, ID-
Centrifuge 12SII, or ID-Centrifuge 24S and who are qualified in accordance with the manufacturer’s
requirements and who are consequently authorized.

Technical personnel

The term «technical personnel» designates the duly trained persons, who are allowed to perform
specific tasks on the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S.

For instance, an electrician is designated as technical personnel for the activities linked to wiring the
ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S to the electrical network.

Personnel qualifications

Several kinds of personnel qualifications are required in order to perform the activities related to the ID-
Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S. These qualifications are described
in the corresponding sections of the present User Manual.

The personnel qualifications define the minimal requirements which must be met by the authorized
personnel.

Serious injury

A serious injury is an injury (ICAO definition) which is sustained by a person in an accident and which:

• requires hospitalization for more than 48 hours, commencing within seven days from the date the
injury was received;
• results in a fracture of any bone (except simple fractures of fingers, toes, or nose);
• involves lacerations which cause severe hemorrhage, nerve, muscle or tendon damage;
• involves injury to any internal organ;
• involves second or third degree burns, or any burns affecting more than 5 per cent of the body
surface;
• involves verified exposure to infectious substances or injurious radiation.

Light injury

Any injury not corresponding to the definition of a serious injury is considered as a light injury.

1.2.2 Product

This is the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S distributed by the
manufacturer.

Centrifuges

The term «centrifuge» is used for the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge
24S manufactured by DiaMed GmbH, CH-1785 Cressier sur Morat.

If the information refers exclusively to a specific type of centrifuge, this is indicated explicitly in the text.

8 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Generalities

1.3 Typographical conventions


The following styles are used in this manual.

1.3.1 Description

This style, use in conjunction with illustration numbers, is preceded with the corresponding numbers:

Example:

(1) First element


(2) Second element
(3) Etc...

1.3.2 Command

Any software command, button, function key, window, icon, option, tab, checkbox, selection box,
article, menu, tool bar, field and section used in this document is represented by a bold italic font.

Example:

The Exit command allows to quit the software.

1.3.3 Procedure

Each procedure step to be carried out step-by-step by the user is preceded by a letter.

Example:

A. Open the drawer.


B. Put the microplate into position as shown.
C. Close the drawer.

1.3.3.1 Procedure result


A procedure result is shown by the following symbol .
Example:

A. Click on the Parameters button.

 The parameter window is displayed.

1.3.4 Cross reference

This style is used to help the user find complementary information linked to the current subject.

Example:

 See section “4.2.4 Sensor Board Positioning” on page 27.

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Generalities

1.3.5 List of items

This style is used in order to display a list of elements.

Example:

• item 1;
• item 2;
• item 3.

1.3.6 Troubleshooting

The complete description along with the error message, the explanation and the remedy appears as
follows:

Problem
- Explanation, possible cause

 Remedy

1.3.7 Warnings

In relation to the importance of the warning and its related risks, three warning styles are defined.

The safety aspects are used in accordance with the requirements contained in the following norms:

• ANSI Z535.4;
• ISO 3864, ISO 3864-1:2002, and ISO 3864-2:2004.

10 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Generalities

1.3.7.1 Danger
Used to designate an imminent and dangerous situation which, if not avoided, may lead to death or
serious injury.

The primary risk is given in capital letters below the DANGER symbol. However, mentioning a specific
risk does not exclude the presence of subsidiary risks.

Example:

DANGER
ELECTROCUTION
Never touch an exposed electrical wire. A contact with an electrical wire may cause an
electrocution.

1.3.7.2 Warning
Used to designate a potentially dangerous situation which, if not avoided, may lead to death or serious
injury.

Example:

WARNING
Always disconnect the electrical cord before opening the instrument. A contact with an
exposed electrical wire may cause an electrocution.

1.3.7.3 Caution
Used to designate a potentially dangerous situation which, if not avoided, may lead to light injury or
cause equipment damage.

Example:

CAUTION
Do not manipulate a broken mirror with bare hands as this operation may result in
cuts.

The use of the CAUTION sign without the warning triangle means that the only risk consists in
equipment damage.

Example:

CAUTION
The use of other cleaning agents or hard objects may damage the instrument. Do not use
other cleaning agents or products without obtaining first the authorization of the
manufacturer.

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Generalities

1.3.8 Recommendation and Note

When complementary information is required and if their non-compliance only leads to minor
inconveniences, recommendations and notes are given.

1.3.8.1 Recommendation
Used to designate a preferred procedure or a recommended practice. Bio-Rad denies any
responsibility in case of non-compliance with the recommendations.

Example:

RECOMMENDATION

Check that the instrument is closed before switching it on.

1.3.8.2 Note
Used to accompany a general remark or a purely informative comment.

Example:

NOTE
The reassembly of the instrument is performed in the opposite order of its disassembling.

12 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Safety and handling 2

Chapter overview

This chapter defines the safety instructions which guarantee a safe and trouble-free operation of the
ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S.

2.1 Introduction
2.1.1 Principle

WARNING
The user must have read and understood this chapter before he performs any operation
on the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S instrument.

In case of unclear information, please contact the manufacturer or your Bio-Rad dealer.

2.1.2 Importance of the safety instructions

Every safety and protection instruction which can be found in this manual must be adhered to in order
to avoid personnel injuries, property damages or environmental pollution.

In a similar manner, the legal bylaws, the measures in prevention of accidents and for the protection
of the environment, as well as the recognized technical rules aiming at appropriate and safe working
conditions which are applied in the country and at the place of use of the ID-Centrifuge 6S / 6S
Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S instrument must be adhered to.

2.1.3 Non-compliance with the safety rules

Disregarding the safety rules, as well as existing legal and technical regulations, may lead to accidents,
property damages or to environmental pollution.

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Safety and handling

2.2 Environmental conditions


WARNING
The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S must not be
located near a water tap or any other source of water.

This system may only be used in closed rooms and never in the immediate environment of
patients.

The electrical safety of the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-
Centrifuge 24S instrument is only guaranteed if the electrical installation is conform to the
reglementation related to medical-use buildings and laboratories and if this installation
works properly.

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S may not be used
in buildings prone to explosion hazards.

CAUTION
The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S must be kept
away from any interference source.

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S may not be
stored under exposure to the direct sunlight, to heat, to dust or to an excessive humidity
(only use the instrument in a clean laboratory environment).

14 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Safety and handling

2.3 General safety instructions

DANGER
Electrocution
During maintenance operations, when the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII,
or ID-Centrifuge 24S powered and uncovered, the instrument may not be left
unmonitored.

DANGER
INFECTION
There is a risk of infection from skin contact with blood samples. Always wear protective
gloves when working, in accordance with laboratory safety regulations.

WARNING
The initial operation may only be carried out by qualified persons who, as a result of their
training and experience, are familiar with the initial operation and the operation of
laboratory instruments.

Maintenance and repairs may only be performed in conformance with the instructions and
by the technical personnel authorized by the manufacturer.

The sole possession of the Service Manual does not allow the personnel to perform any
kind of repair on the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge
24S.

Take into account all the warnings and follow all the instructions displayed on the ID-
Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S and which are printed
in the documentation.

It is mandatory to use the products specified in the present User Manual to clean the ID-
Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S. If you plan to use
another product, only do so after having obtained the authorization of the manufacturer.

Using materials other than those defined in the User Manual (unauthorized dangerous
goods for instance) is forbidden. Breaking this rule will be considered by the manufacturer
as guilty negligence.

Never try to use replacement pieces other than those authorized by the manufacturer of
the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S.

The centrifuge must not be operated with an open protective lid due to the risks of injury
when the head is rotating.

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Safety and handling

CAUTION
The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S must be used
on an appropriate table (See section “4.1 Unpacking and setting up the ID-Centrifuge” on
page 27).

Ensure that the ventilation around the ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or
ID-Centrifuge 24S is sufficient to avoid any excessive heating. A space of 300 mm all
around the instrument must be left clear of any obstacle.

The centrifuges must only be used with accessories approved by the manufacturer
(example: ID centrifuge head, ID-Card holder).

Do not operate the centrifuge in the case of damage or switch off immediately. Switch off
the power switch and remove the power plug from the power supply! Mark the centrifuge
as defective to prevent further use.

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S may not be used
in combination with other machines. The manufacturer’s agreement must be obtained
before using the instrument in conjunction with other equipment.

Use the cleaners mentioned in the User Manual for cleaning the centrifuge. Other cleaners
should be used only after consulting the manufacturer.

The incubation of materials other than those described in the User Manual is forbidden
(e.g. tubes with sample material). Any disregard is considered gross negligence by the
manufacturer.

Switch off the power switch and remove the power cord from the power supply when
cleaning the centrifuge or when replacing the fuses.

Repairs must be carried out by authorised personnel.
Do not try to start up the centrifuge yourself (except when indicated by directions in the
User Manual). Owning a Service Manual does not mean you are authorised to carry out
repairs on the centrifuge. The manufacturer offers special training courses for repairing
the instrument. You can obtain information from your Bio-Rad representative or
manufacturer.

This User Manual must always be accessible to all personnel working with the ID-
Centrifuge.
Never let any liquid enter the system. In case of liquid spill inside the instrument, act
immediately as follows:

A. Switch off the instrument using the main switch.


B. Unplug the power cord.
C. Dry up the instrument.
D. Clean and decontaminate the instrument.
E. Check all its electrical functions.

RECOMMENDATION

For further information, please contact your Bio-Rad dealer or the manufacturer.

16 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Safety and handling

2.3.1 Observations and information

In case of malfunction or any other technical incident for which a remedy has not been described in
the user manual, please contact the manufacturer immediately.

Safety measures ordered by the manufacturer are mandatory.

2.4 Special safety instructions


Safety instructions are inserted at certain points in this manual to indicate special hazards which occur
only in certain situations.

They should be observed in the same way as the general safety instructions contained in this chapter.

2.5 Storage conditions


2.5.1 Deactivation and storage

Deactivation is normally performed to transport the centrifuge to a different place inside or outside the
laboratory, for repair in an internal or external workshop or for storage for later use.

WARNING
To prevent danger to any persons during deactivation and during the inactive state, the
centrifuge must be decontaminated before being transported to another place.

A. Decontaminate the centrifuge as described in section “6.5 Decontamination” on page 55.


B. Fill in a decontamination certificate after decontamination. You can find a form for the decontamination
certificate in the appendix at the end of this manual.
C. Make a copy of the decontamination certificate and keep the original together with the centrifuge.
D. Note the instructions in chapter “2.5.2 Handling and transportation” on page 18 before transporting the
centrifuge.

2.5.1.1 Storage
If the centrifuge is temporarily stored in a laboratory environment, complete packing is normally not
required.

However, if the centrifuge is stored in a storage room, basement or similar area for a longer period of
time, we recommend that you pack it completely to protect it.

Centrifuges must be packed for storage as described in chapter “2.5.2 Handling and transportation” on
page 18.

RECOMMENDATION

Use only the original packing to pack the centrifuge.

For storage conditions, see chapter “3.5.5.2 Storage” on page 25.

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Safety and handling

2.5.2 Handling and transportation

RECOMMENDATION

Use only the original packing for transport and shipping.

2.5.2.1 Packing

CAUTION
Packing is required particularly when shipping or for the long-term storage of the centrifuge.

A. Before packing the centrifuge carry out a complete shut-down.


B. Reconnect the centrifuge to the power network.
C. Turn on the centrifuge and open the lid.
D. Turn off the open centrifuge. Pull the power lead out of the appliance socket and out of the power
supply socket.
E. Place the packing and the centrifuge head in the tub of the centrifuge.
F. Close the lid so that you hear a click.
G. Place the centrifuge with its packing material into the transport box.
H. Place all accessories in the box.
I. Place all necessary papers in the box:
• delivery note with complete information;
• decontamination certificate.
2.5.2.2 Packing list
The box should contain the following equipment:

• 1 instrument (6S / 6S Accu, 12SII, or 24S)


• 1 power cord (CH, EURO, UK or US)
• 1 User Manual
• 4 O-rings (only 12SII)
• 2 spare filters for fan (only 12SII and 24S)
• 1 declaration of conformity
• 1 unlocking pin (only 24S)
• 1 reception form
• 1 installation protocol
2.5.2.3 Transportation recommendation
For domestic transportation, it is recommended to use: car, truck, postal parcel and train.

For international and overseas transportation, it is recommended to use a professionnal shipping


company.

NOTE
During transportation, make sure that all the instructions on the parcel are adherred to

18 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Description 3

Chapter overview

This chapter describes the design and function of individual modules of the ID-Centrifuge 6S / 6S
Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S including the operating and display panels as well as
the basic principles of the process.

3.1 Principles of the process


3.1.1 Basic principles

The ID-System is based on a process for determining antigen and antibody complexes (EU Patent
0305337). Agglutinated particles are separated from non-agglutinated particles by means of an inert
gel. ID-Cards containing six microtubes filled with gel are used.

The reaction takes place in the microtubes prefilled with gel. The gel suspension contains the specific
antibodies, reagents or buffer mixtures depending on the determination to be performed.

(1) ID-Card
(2) Microtubes (4)
(2)
(3) Gel column
(4) Opening to reaction chamber
(5) Identification label (3)

After adding the test material, the separation


(1)
between agglutinated and non-agglutinated
erythrocytes can be visually observed in the gel
column after the centrifugation that follows.
Depending on the intensity of the reaction,
(5)
erythrocytes penetrate the gel to varying degrees
and reactions can be graded as follows: Fig. 3-1 ID-Card description

Fig. 3-2 Degrees of reaction

The reactions remain visible in an unchanged state for several days and can be clearly assessed.

The methodology and reactions are standardized. High precision is required with respect to
centrifugation time and speed is to ensure the quality of the analyses.

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Description

3.1.2 ID-Cards used

Only ID-Cards may be used in the centrifuges. There are different types of cards available:

• ID-Cards with gel which contains specific antibodies to determine erythrocyte antigens (blood
groups antigens and other rare antigens);
• ID-Cards with gel which contain the anti-human globulin (mono-/polyspecific for performing anti-body
search tests, anti-body identification, tolerance test and to determine the direct Coomb tests;
• ID-Cards with neutral gel to determine the serum check, NaCl and the enzyme test.
Detailed information on the ID-Cards available is contained in the Sales Catalog for the ID-System.

3.2 General description


3.2.1 Overview and designation of the main parts

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S have more or less the
same design. They mainly differ in the number of ID-Cards which they can process at the same time.

(4)
(3)
(2)
(1)

(5)

(6)
(7)

Fig. 3-3 Main elements

(1) Housing
(2) Tub
(3) Drive spindle
(4) Cover
(5) Operating panel
(6) Centrifuge head
(7) ID-Card

20 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Description

3.3 System description


3.3.1 Power socket, equipment fuse, and power switch

(1) Power switch:


I = ON
O = OFF
(2) Equipment fuses
(3) Equipment plug (1)
(4) Power cord (2)

(5) Equipment plug (3)


(4)
(6) Power plug (Swiss, Euro, UK or US version)

(5)
(6)

Fig. 3-4 Power socket, fuse and power switch

3.3.2 6S / 6S Accu control panel

All controls, with the exception of the power


switch mentioned above, are grouped on the
front control panel. The keys on the operating
panel are for direct control; the three LEDs
provide information on the current status of the
centrifuges.
(1) (2) (3)
START button:  Fig. 3-5 ID-Centrifuge 6S / 6S Accu, control panel
Starts centrifugation.

STOP/OPEN button: 
Stops the centrifuge and opens the lid when the
centrifuge has stopped.

POWER LED: 
Lights up when the power supply is on.

READY LED: 
Lights up or flashes depending on the current
operating state.

ALARM LED: 
Lights up when fault.

The LEDs have 3 states:

(1) LED on
(2) LED flashing
(3) LED off

H009120 V13.0-01/2019 21
Description

3.3.3 12SII and 24S control panels

All controls, with the exception of the power


switch, on the centrifuges 12SII and 24S are
grouped on the front operating panel. The
buttons on the operating panel are for direct
Fig. 3-6 ID-Centrifuge 12SII, control panel
control of the centrifuges. The LCD provides
information in plain text on the current status of
the centrifuges.

START button: 
Starts centrifugation.

STOP/OPEN button: 
Fig. 3-6 ID-Centrifuge 24S, control panel
Stops the centrifuge and/or opens the lid when
the centrifuge has stopped.

LCD: 
Alphanumeric text display for the operating
status, 16 characters.

3.3.4 Acoustic signals

The displays on the control panel of the


centrifuge are supported by an in-built signal
transmitter. This tells the operating personnel that
centrifugation has ended or that there are
operating faults even when they cannot see the
control panel.

3.3.4.1 Centrifuge 6S / 6S Accu


• every press of the button is acknowledged by
one beep;
• blocked head alarm with Beethoven melody;
• start without head alarm with Beethoven
melody;
• end of program signal with two-tone horn.

3.3.4.2 Centrifuge 12SII and 24S


• every press of the button is acknowledged by
one beep;
• alarm for centrifugation interruption due to
fault: two-tone horn;
• excessive vibration alarm with Beethoven melody;
• end of program signal with two-tone horn.

22 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Description

3.4 Features
3.4.1 Function description

After opening the lid, place the centrifuge head


loaded with ID-Cards on the drive spindle. Then
close the lid. The centrifuge is now ready for
centrifugation. After starting, centrifugation is fully
automatic. A centrifugation lasts for 10 minutes.
The process need not be supervised by the
operating personnel.

A stable speed and a precise centrifugation time


are important factors affecting the quality of the
examinations. These parameters are monitored
and controlled by the internal microprocessor.

The following overview shows and names


different modules and accessories from the
centrifuge.

During rotation, the ID-Cards in the centrifuge


head are swiveled through 90 degrees in order to
generate a defined centrifugal force which acts
evenly on all samples in the microtubes of the
ID-Cards.

The uniform effect of the centrifugal force is very


important for the quality of the examinations.
Therefore, on centrifuges 12S ll and 24S, a
vibration sensor monitors the smooth running of
the centrifuge head.

If centrifugation is interrupted prematurely by the


microprocessor due to speed, centrifugation time
or vibration errors, the centrifuge generates an
appropriate error message. The results of the Fig. 3-7 ID-Card during centrifugation
samples are invalid.

Centrifuge 6S / 6S Accu and 12SII

At the end of a trouble-free centrifugation, the lid


opens automatically and the centrifuge generates
a buzzer alert.

The centrifuged ID-Cards can then be removed


from the centrifuge and the test can be
evaluated.

Centrifuge 24S

On the centrifuge 24S, the lid remains closed for


the operating personnel to open.

The centrifuged ID-Cards can then be removed


from the centrifuge and the test can be
evaluated.

H009120 V13.0-01/2019 23
Description

3.5 Technical data


3.5.1 Performance data

Performance data Unit 6S / 6S Accu 12SII 24S

Number of ID-Cards - 6 12 24
Nominal speed min-1 1175 1030 910
Tolerance on speed min-1 ± 5 ± 5 ± 5
Nominal acceleration g 85 85 85
Tolerance on acceleration g ± 1 ± 1 ± 1
Nominal centrifugation time min 10 10 10
Tolerance (centrifugation sec ± 12 ± 12 ± 12
time)

3.5.2 Transport data

Weight
Type Size WxHxD [cm]
[kg]

6S / 6S Accu 41x41x32 8.1 H

12SII 46x46x32 6.5


24S 52x52x32 14.4
D W

Fig. 3-8 Space requirements

3.5.3 Physical data

Physical data Unit 6S / 6S Accu 12SII 24S

Supply voltage VAC 100...240 100...240 100...240


Power frequency Hz 50...60 50...60 50...60
Max. power draw VA 40 55 70
Power supply fuses A 2 (slow blow) 2 (slow blow) 2 (slow blow)
(100 - 240 VAC)

24 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Description

3.5.4 Dimensions and weights

B A

Fig. 3-9 Dimensions

Dimensions and weights Unit 6S / 6S Accu 12SII 24S

A = Width cm 27 30 37
B = Depth cm 32 36 42
C = Height cm 17 18 18
D = Height with lid open cm 43 45 53
Weight of centrifuge kg 6.6 5.5 11.1
Weight of centrifuge head kg 0.29 0.65 1.2

3.5.5 Environmental conditions

3.5.5.1 Operational conditions


Unit
Operating ambient temp. °C ...................+5 ... +40
Ambient air humidity
(Non-condensing) % ....................20 ... 90

3.5.5.2 Storage
Storage ambient temp. °C ....................-10 ... +65
Ambient air humidity
(Non-condensing) %......................20 ... 90

3.5.6 Emissions

Noise dB(A) ...............< 70

H009120 V13.0-01/2019 25
Description

3.6 Commercial information


The complete instrument described in the present user manual can be ordered to the manufacturer

DiaMed GmbH
Export service
CH-1785 Cressier-sur-Morat

Phone: +41 26 67 45 111

These instruments bear the following article numbers:

Type Art. #

ID-Centrifuge 6S ..............................................009560
ID-Centrifuge 12SII .........................................009550
ID-Centrifuge 24S ............................................009530

26 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Commissioning 4

Chapter overview

This chapter describes the unpacking and setting up of the ID-Centrifuge as well as its connection to
the power supply. All other preparative steps necessary for normal operation of the centrifuge are also
described.

WARNING
The safety regulations contained in chapter “2 Safety and handling”, as well as all the
safety regulations contained in this chapter must be applied.

NOTE
The displays shown in this chapter are only examples. They do not necessarily correspond with the
text on your control panel.

4.1 Unpacking and setting up the ID-Centrifuge


4.1.1 Installation site

Choose a stable, flat surface for the centrifuge, such as a laboratory table.

CAUTION
Do not place the centrifuge on a shelf or on a trolley. The centrifuge must be protected
against direct sunlight, large variations in temperature and humidity.

A power supply connection must be available at the installation site and it must be possible to reach
this connection with the power lead supplied. Extension leads are not suitable.

The installation site must be sufficiently well lit for the use of the centrifuges.

Moreover, in order to ensure optimal handling and smooth operation, the installation site of the
centrifuge should be chosen such that the following conditions are met:

4.1.1.1 Environmental conditions


For numerical values, section “3.5.5 Environmental conditions” on page 25

DANGER
ELECTRIC SHOCK
The ID-Centrifuges are not protected against water entry. This can lead to an electric
shock. Do not place the centrifuge in the vicinity of running water or anywhere where
water may enter.

DANGER
FIRE OR EXPLOSION
The ID-Centrifuges are not protected against explosions. Sparks could cause a fire or
explosion. Do not place the centrifuge in the vicinity of inflammatory or explosive
substances.

H009120 V13.0-01/2019 27
Commissioning

CAUTION
The centrifuge may overheat if sufficient air circulation is not provided. This may cause
malfunction or complete breakdown of the centrifuge. Make sure the area between the
bottom of the centrifuge and the work top and the safety area of at least 300 mm around
the centrifuge is kept free.

A safety area of at least 300 mm should be kept


free on both sides and behind the centrifuge in
order to provide easy access for the power
connection as well as sufficient air circulation.

Fig. 4-1 Safety area

The entire safety zone area must be left free


above the centrifuge, from the surface of the
table upwards, in order to facilitate free access
for operation.

Any shelves above the centrifuge must particularly


be avoided, as items may fall off these onto the
centrifuge.

Fig. 4-2 Safety area

28 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Commissioning

4.1.2 Unpacking

NOTE
The centrifuge weighs less than 13 kg and can generally be handled with ease by one person.

A. Remove all accessories from their packing.

RECOMMENDATION

Do not damage the packaging when unpacking the centrifuge and keep it in a safe place for later
use, such as storage or transport.

B. Ensure that all items are present. The box should contain the following accessories:
• 1 power cord (CH, EURO, UK or US)
• 1 User Manual
• 4 O-rings (only 12SII)
• 2 spare filters for fan (only 12SII and 24S)
• 1 declaration of conformity
• 1 unlocking pin (only 24S)
• 1 reception form
• 1 installation protocol
C. Lift the centrifuge out of the box.
D. Remove the upper part of the packaging from the centrifuge.
E. Turn the centrifuge around (180°) and remove the rest of the packaging.
F. Place the centrifuge in an upright position (cover facing upwards) on the work top.

CAUTION
Check the centrifuge for any exterior damage. If you find any damage, inform your Bio-Rad
representative immediately.

RECOMMENDATION

The centrifuge head is packed for transportation in the tub of the centrifuge with special
transportation safety devices. The centrifuge head can only be unpacked once the centrifuge has
been connected to the power supply and turned on for the first time.

H009120 V13.0-01/2019 29
Commissioning

4.1.3 Connecting the ID-Centrifuge to the power network

DANGER
ELECTRIC SHOCK
The ID-Centrifuge should be used only with the 3-pole power cord supplied. If the ground
conductors are bypassed, an electric shock may occur. For reasons of safety the power
plug will fit only in grounded wall sockets. If the power plug does not fit into the wall
socket the power cord must be changed accordingly by an electrician.

WARNING
Risk of tripping
The power cord must not be hindered by any objects. Route the power cord so that people
can not tread or trip over the cable.

A. Make sure the power switch is set to O. The


side of the rocker switch marked O must be
pressed in.
B. First plug the equipment plug of the power cord
firmly into the socket on the rear of the
centrifuge.
C. Then insert the power plug firmly into the
grounded wall socket.

4.1.4 Unpacking the centrifuge head

A. Switch on the centrifuge and test its operating


status. The centrifuge reacts as follows:

 6S / 6S Accu
The POWER and READY LEDs light up.

Fig. 4-3 6S / 6S Accu, control panel

 12SII and 24S


The following message appears on the
LCD.
Ready

Fig. 4-4 12SII and 24S, control panel

30 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Commissioning

B. Press the STOP/OPEN button to open the lid.

Fig. 4-5 Opening the lid

C. Lift the centrifuge head and its packaging


carefully out of the tub.

RECOMMENDATION

Carefully keep all of the centrifuge head


packaging for later use. This packaging must be
used when transporting centrifuges containing a
centrifuge head.

D. Now clean the empty tub of the centrifuge using


a dry cloth, until all residual scraps of packing
have been cleanly removed.

CAUTION
Do not use any solvent or other liquid for
cleaning purposes apart from a 70% ethyl
alcohol solution and use even this extremely Fig. 4-6 Lifting the centrifuge head
sparingly. Further information on this can be
found in the section “6.3.1 Cleaning agents”
on page 53.

E. Switch off the centrifuge.


F. Replace the centrifuge head into position.
G. Close the lid of the centrifuge, ensuring that it
locks into position.
H. Now bring the centrifuge into its final position at
the installation site.

H009120 V13.0-01/2019 31
Commissioning

4.2 Initial operation


Prior to the commencement of normal operation with the centrifuge, the initial operation measures
described below should be taken. These measures involve configuration, checking the target speed,
checking the centrifugation time and keeping a maintenance record.

4.2.1 Configuration

4.2.1.1 Centrifuge 6S / 6S Accu


The 6S / 6S Accu has no LCD. For this reason, no configuration is required. Please continue reading
section “6.6.3 Checking the target speed” on page 57.

4.2.1.2 Centrifuge 12SII and 24S

CAUTION
Before starting configuration, the centrifuge must be turned off and the lid must
be closed and locked.

NOTE
If you make a mistake when configuring the general parameters, finish the procedure and then
return to the start to correct the erroneous setting. When repeating this process, you can
immediately acknowledge all of the settings which do not need to be changed and you can
therefore move quickly from one configuration stage to the next.

The configuration is carried out in stages. The following parameters can be configured:

• direction of rotation of the centrifuge cw =


right, or ccw = left. The direction of rotation is
generally clockwise (cw = clockwise);
Language
• languages of the display available:
Contrast
- German,
- French,
- English, CW CCW
- Spanish,
- Italian, and
- Portuguese.
• LCD contrasts available:
1...40 low, 
41...80 normal, 
81...99 strong.
• Beep: on or off.
The configuration is carried out in stages and in
a specific sequence. All settings are made using beep
both the START and the STOP/OPEN
buttons.

• START Step-by-step change of the Fig. 4-7 Centrifuge configuration


parameters;
• STOP/OPEN Confirmation of the setting
displayed and switching to the next
configuration stage.

32 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Commissioning

4.2.2 Configuration procedure

4.2.2.1 Starting the configuration


A. Switch on the power switch at the rear of the
centrifuge.


Ready
The following message appears on the
LCD:

Fig. 4-8

B. Press STOP/OPEN to open the lid.

 The following message appears on the Lid is open


LCD:

Fig. 4-9

C. Press the STOP/OPEN button for about 2


seconds.
System Set-up

Fig. 4-10

 The following messages appear in


succession on the LCD:
To change : Start

Fig. 4-11

To save : Stop

Fig. 4-12

 The last message remains. It means that


the centrifuge head is turning in the
clockwise (cw) direction. You can now Direction : cw
either confirm or change the direction of
rotation. The normal direction of rotation is
counterclockwise (ccw).
Fig. 4-13

H009120 V13.0-01/2019 33
Commissioning

4.2.2.2 Changing the direction of rotation


A. Press the START button.

 The following message appears on the Direction : ccw


LCD:

NOTE Fig. 4-14

This message means that the centrifuge head is


now turning in the counterclockwise (ccw)
direction.

B. Confirm the displayed direction of rotation by


pressing the STOP/OPEN button.


Lang. English
The following message appears on the LCD:

NOTE Fig. 4-15

The message means that all texts will appear on the


LCD in English. You can now either confirm or
change the language.

4.2.2.3 Changing the language


A. Press the START button until the required
language appears on the LCD.
B. Confirm the displayed language by pressing the Lang. English
STOP/OPEN button


Fig. 4-16
The following text appears on the LCD:

 The message means that the LCD is set Contrast : 49


to the contrast stage 49 in a range
between 1 - 40 low, 41 - 80 normal, 81 -
99 high. You can now confirm or change
the setting. Fig. 4-17

4.2.2.4 Changing the contrast setting


A. Press the START button until the required
contrast stage appears on the LCD. Contrast : 70
B. Confirm the displayed contrast stage by press
the STOP/OPEN button.


Fig. 4-18
The following text appears on the LCD:

NOTE Alarm : ON

The message means that the beep is on. You


can then confirm or switch off the setting for the
beep. Fig. 4-19 Text displayed

34 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Commissioning

4.2.2.5 Setting the beep


A. Press the START button until the required alarm
setting appears on the LCD.
B. Confirm the displayed alarm setting by pressing Alarm : OFF
the STOP/OPEN button.

Fig. 4-20

 The following text appears on the LCD:

NOTE * End *

The configuration procedure is then completed.

Fig. 4-21

H009120 V13.0-01/2019 35
Commissioning

4.3 Faults during the initial operation


The faults which can occur during the initial operation are identical to the faults which arise during
operation.

4.3.1 Rectifying faults

You will find a full description of possible faults and the measures which must be taken to rectify
these faults in chapter “7 Troubleshooting”.

4.3.2 Keeping a maintenance record

The manufacturer recommends starting a maintenance record for every centrifuge at the time of the
initial operation. This log should be used to record all occurrences, such as all of the checks which
are carried out, maintenance and replacement work and all necessary repair work carried out on the
centrifuge.

A photocopying template with a form for keeping a maintenance record can be found in the appendix
at the end of this User Manual.

All of the necessary information concerning the initial operation can be entered in the spaces provided
on this form, together with all subsequent notes.

Whenever repair work is necessary, the centrifuge should be accompanied by the completed
maintenance record, in order that the repair personnel can have access to this. This can reduce the
necessary repair time considerably.

36 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation 5

Chapter overview

This chapter describes the normal operation of the centrifuge. The maintenance work necessary for
smooth functioning is described in chapter “6 Maintenance”.

DANGER
BIOHAZARD
Infection may occur due to skin contact with blood samples. Blood remains spun off
during operation of the cellwash centrifuge may be present in the tub or inside of the lid.
Wear safety gloves according to laboratory safety stipulations.

WARNING
The ID-Centrifuge may be operated only by persons instructed in their handling.
Observe chapter “2 Safety and handling” as well as all safety instructions contained in this
chapter.
If malfunctions occur, consult chapter “7 Troubleshooting”.

NOTE
Bio-Rad provides beginner and advanced courses for users. For detailed information contact your
Bio-Rad agent.

NOTE
The displays shown in this chapter are only examples. They do not necessarily correspond with the
text on your operating panel.

H009120 V13.0-01/2019 37
Operation

5.1 Centrifuge 6S
5.1.1 Normal procedures

5.1.1.1 Switching on the ID-Centrifuge

DANGER
ELECTRIC SHOCK
If operated or handled incorrectly, electrical equipment can lead to an electric shock. 
Never touch electrical equipment or parts of them, e.g. power cord, power plug or sockets
with wet hands.

A. Turn on the power switch at the rear of the


centrifuge. At the end of the self test, the
centrifuge indicates it is ready to operate.

 6S / 6S Accu
The POWER and READY LEDs light up.

B. Press the STOP/OPEN button.

 The lid opens.

Fig. 5-1 6S / 6S Accu centrifuge

5.1.1.2 Loading the centrifuge head


C. Load the centrifuge head using the ID-Cards to
be processed.

Fig. 5-2 6S / 6S Accu: Loading the centrifuge head

CAUTION
Load the centrifuge head evenly with ID-
Cards. On centrifuges 12S ll and 24S this
will ensure that centrifugation is not
automatically interrupted by the integrated
vibration sensor.

WARNING
An interrupted test must be repeated with
new samples

38 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

5.1.1.3 Loading options for the centrifuge head


If you must load less than the maximum number
of ID-Cards, you must use the following loading
options.

Fig. 5-3 Centrifuge 6S / 6S Accu loading options


(2, 3, or 4 ID-Cards)
5.1.1.4 Inserting the centrifuge head
D. Place the centrifuge head on the drive spindle
and slide it down until the bottom is reached
audibly. The driver pin in the centrifuge head then
slides automatically into the groove of the drive
spindle.

Fig. 5-4 6S / 6S Accu: Inserting the centrifuge head

5.1.1.5 Performing a centrifugation


E. Close the lid until it engages audibly. It is then
automatically locked.

 The centrifuge indicates it is ready to


operate by lighting up the POWER and
READY LEDs.
Fig. 5-5 6S / 6S Accu: Centrifuge ready

F. Press the START button. The centrifuge


accelerates the centrifuge head to nominal speed.
When the nominal speed is reached, the
centrifugation time of 10 minutes starts.

 Centrifugation starts when the READY LED


starts to flash. During the centrifuge time of
10 min, the READY LED flashes slowly for
8 minutes. For the last 2 minutes, the LED
flashes at a faster rate.

NOTE Fig. 5-6 6S / 6S Accu: Centrifuge running


If there is no fault, the centrifugation runs fully
automatically after the start. The centrifuge need
no longer be operated or supervised.

H009120 V13.0-01/2019 39
Operation

G. At the end of the centrifugation, the head drive


switches off automatically.

 As soon as the centrifuge head has come


to rest, the lid on the centrifuge opens
automatically. 
The 2-tone horn sounds. The POWER LED
lights up and the lid opens. Fig. 5-7 6S / 6S Accu: Centrifugation completed

5.1.2 Emergency stop

If centrifugation must be stopped prematurely for


any reason (emergency stop), press the STOP/
OPEN button. This immediately interrupts a
centrifugation and stops the centrifuge head from
rotating.

When centrifugation is interrupted by pressing the


STOP/OPEN button, the 2-tone horn sounds Fig. 5-8 6S / 6S Accu: Centrifugation interrupted by
and the lid opens. personnel

WARNING
When centrifugation is interrupted by the
operating personnel, the test is invalid. You
must repeat the test using new samples.

5.1.3 Other abnormal procedures

5.1.3.1 Centrifugation interrupted by the centrifuge


Acceleration, vibrations and the nominal speed
are automatically monitored by the centrifuge. If
the centrifuge detects a fault, the started
centrifugation is interrupted automatically and the
centrifuge signals this by the audible and visual
alarms for centrifugation interruption.

When centrifugation is interrupted, the Beethoven Fig. 5-9 6S / 6S Accu: Alarm


melody sounds, the ALARM LED comes on when
fault.

ALARM comes on


The nominal speed was not reached or the
centrifuge head is blocked.

WARNING
When centrifugation is interrupted by the
centrifuge, the test is invalid. You must
repeat the test using new samples.

40 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

5.2 Centrifuge 6S Accu


CAUTION
Please read these instructions carefully before using the centrifuge for the first time !

NOTE
The ID-Centrifuge 6S Accu is operated in the same manner as the ID-Centrifuge 6S, with the
exception of the instructions contained on the present section

5.2.1 Normal operations

The centrifuge can be used with 3 different power sources:

• Power supply (100-240V)


• Battery package (12V)
• External 12V supply

CAUTION
The battery package may only be charged with the delivered battery charger (Bosch AL 60 DV)

With a fully charged battery approx. 35 cycles can be performed.

The switch (Pos. 0/1) can only be used with the 100-240V power supply.

In the standby position, we recommend that the switch on the front side of the centrifuge be set to
the ext. power position, in order to economize the battery.

5.2.1.1 Operation with the 100-240V power supply


A. Plug the power cable into the rear side of the centrifuge.
B. Plug the other end of the cable into the mains connection.
C. Set the switch on the rear side of the centrifuge to position 1.
D. The yellow power lamp lights up meaning that the centrifuge may be operated.

H009120 V13.0-01/2019 41
Operation

5.2.1.2 Operation with the battery package


Battery not yet integrated

A. Open the plexiglas cover on the back side with the snap locks.
B. Connect the charged battery my means of the snap connector.
C. Place the battery into its holder.
D. Fix the plexiglas cover.
E. Set the switch on the front side of the centrifuge to the accu position, after which the centrifuge may
be operated (the power and ready lamp lighten up when the lid is closed).
With the integrated battery

A. Set the switch on the front side of the centrifuge in the accu position, after which the centrifuge may
be operated.
Operation with the external 12V power supply

A. Set the switch on the front side of the centrifuge to the ext. power position.
B. Connect the 12V supply cable to the foreseen connector on the rear side of the centrifuge and
connect the power cable after which operation may begin.

5.2.1.3 Charging the battery with the Bosch AL 60 DV charger


A. Connect the battery connector to the battery charger connector.
B. Connect the charger to the power supply (100-240V).
C. During the charging phase the centrifuge green lamp blinks.
D. When the battery has been fully charged the green lamp lights up continuously.
E. A complete charging cycle lasts approx. 4 hours.

5.2.2 Emergency stop

 See section “5.1.2 Emergency stop” on page 40

5.2.3 Other abnormal procedures

 See section “5.1.3 Other abnormal procedures” on page 40

42 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

5.3 Centrifuge 12SII


5.3.1 Normal procedures

5.3.1.1 Switching on the ID-Centrifuge

DANGER
ELECTRIC SHOCK
If operated or handled incorrectly, electrical equipment can lead to an electric shock. 
Never touch electrical equipment or parts of them, e.g. power cord, power plug or sockets
with wet hands.

A. Turn on the power switch at the rear of the


centrifuge. At the end of the self test, the
centrifuge indicates it is ready to operate.

 The following text appears on the LCD:

B. Press the STOP/OPEN button. Ready


 The lid opens.

Fig. 5-10 12SII centrifuge

5.3.1.2 Loading the centrifuge head


C. Load the centrifuge head using the ID-Cards to
be processed.

CAUTION
Load the centrifuge head evenly with ID-
Cards. This will ensure that centrifugation is
not automatically interrupted by the
integrated vibration sensor.

Fig. 5-11 12SII: Loading the centrifuge head

WARNING
An interrupted test must be repeated with
new samples.

5.3.1.3 Loading options for the centrifuge head


If you must load less than the maximum number
of ID-Cards, you must use the following loading
options.

Fig. 5-12 Centrifuge 12SII loading options


(4, 6, or 8 ID-Cards)

H009120 V13.0-01/2019 43
Operation

5.3.1.4 Inserting the centrifuge head


D. Place the centrifuge head on the conical drive
spindle and gently press it down completely.

5.3.1.5 Performing a centrifugation


E. Close the lid until it engages audibly. It is then
automatically locked.

Fig. 5-13 12SII: Inserting the centrifuge head

 The centrifuge indicates it is ready to


operate by displaying the following text.
Ready
F. Press the START button. The centrifuge
accelerates the centrifuge head to nominal speed.
When the nominal speed is reached, the
centrifugation time of 10 minutes starts. Fig. 5-14 12SII centrifuge ready

 Centrifugation starts when the timer starts


counting backwards.
1030 rpm ; 9.59
NOTE
If there is no fault, the centrifugation runs fully
automatically after the start. The centrifuge need Fig. 5-15 12SII centrifuge running

no longer be operated or supervised.

G. At the end of the centrifugation, the head drive


switches off automatically.


Process finished
As soon as the centrifuge head has come
to rest, the lid on the centrifuge opens
automatically.
Fig. 5-16 12SII centrifuge after centrifugation
The 2-tone horn sounds and the following
message appears on the LCD.

After 3 to 4 seconds, the lid opens and the


following message appears on the LCD.
Lid is open

Fig. 5-17 12SII centrifuge, lid open

44 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

5.3.2 Emergency stop

If centrifugation must be stopped prematurely for


any reason, press the STOP/OPEN button. This
immediately interrupts a centrifugation and stops
the centrifuge head from rotating.

When centrifugation is interrupted by pressing the


STOP/OPEN button, the 2-tone horn sounds
and the following text appears on the LCD. Interruption

Fig. 5-18 12SII: Centrifugation interrupted

After 3 to 4 seconds, the lid opens and the


following message appears on the LCD:
Lid is open

Fig. 5-19 12SII: lid is open

WARNING
When centrifugation is interrupted by the
operating personnel, the test is invalid. You
must repeat the test using new samples.

H009120 V13.0-01/2019 45
Operation

5.3.3 Other abnormal procedures

5.3.3.1 Centrifugation interrupted by the centrifuge


Acceleration, vibrations and the nominal speed
are automatically monitored by the centrifuge. If
the centrifuge detects a fault, the started
centrifugation is interrupted automatically and the
centrifuge signals this by the audible and visual
alarms for centrifugation interruption.

The nominal speed was not reached or the


centrifuge head is blocked.

Beethoven melody; the following text appears on Low tacho


the LCD.

Fig. 5-20 12SII: Nominal speed not reached

After 3 to 4 seconds, the following message


appears:
Check the head

Fig. 5-21 12SII: Check the head

Unbalanced loading of the centrifuge head.

Beethoven melody; the following text appears on


the LCD. Vibrations

Fig. 5-22 12SII: Vibrations

After 3 to 4 seconds, the following message


appears:
Check the head

Fig. 5-23 12SII: Check the head

WARNING
When centrifugation is interrupted by the
centrifuge, the test is invalid. You must
repeat the test using new samples.

46 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

5.4 Centrifuge 24S


5.4.1 Normal procedures

5.4.1.1 Switching on the ID-Centrifuge

DANGER
ELECTRIC SHOCK
If operated or handled incorrectly, electrical equipment can lead to an electric shock. 
Never touch electrical equipment or parts of them, e.g. power cord, power plug or sockets
with wet hands.

A. Turn on the power switch at the rear of the


centrifuge. At the end of the self test, the
centrifuge indicates it is ready to operate.

 The following text appears on the LCD:

B. Press the STOP/OPEN button. ready


 The lid opens.

Fig. 5-24 24S: Centrifuge ready


5.4.1.2 Loading the centrifuge head
C. Load the centrifuge head using the ID-Cards to
be processed.

CAUTION
Load the centrifuge head evenly with ID-
Cards. This will ensure that centrifugation is
not automatically interrupted by the
integrated vibration sensor.

Fig. 5-25 24S: Loading the centrifuge head

WARNING
An interrupted test must be repeated with
new samples.

5.4.1.3 Loading options for the centrifuge head


If you must load less than the maximum number
of ID-Cards, you must use the following loading
options:

Fig. 5-26 Centrifuge 24S loading options


(4, 8, 12, 16, or 20 ID-Cards)

H009120 V13.0-01/2019 47
Operation

5.4.1.4 Inserting the centrifuge head


D. Place the centrifuge head on the drive spindle.
Then rotate the drive pin until it engages in the
groove of the drive spindle. Then push the
centrifuge head completely down until it engages
audibly.

5.4.1.5 Performing a centrifugation


E. Close the lid until it engages audibly. It is then
automatically locked.


Fig. 5-27 24S: Inserting the centrifuge head
The centrifuge indicates it is ready to
operate by displaying the following text.
Ready
F. Press the START button. The centrifuge
accelerates the centrifuge head to nominal speed.
When the nominal speed is reached, the
centrifugation time of 10 minutes starts. Fig. 5-28 24S centrifuge ready

 Centrifugation starts when the timer starts


counting backwards.
910 rpm ; 9.59
NOTE
If there is no fault, the centrifugation runs fully
automatically after the start. The centrifuge need Fig. 5-29 24S centrifuge running

no longer be operated or supervised.

G. At the end of the centrifugation, the head drive


switches off automatically.


Process finished
When the centrifuge head has come to
rest, the lid remains closed.
Fig. 5-30 24S centrifuge finished.
The 2-tone horn sounds and the following
message appears on the LCD.

5.4.2 Emergency stop

If centrifugation must be stopped prematurely for


any reason, press the STOP/OPEN button. This
immediately interrupts a centrifugation and stops
the centrifuge head from rotating.

When centrifugation is interrupted by pressing the


STOP/OPEN button, the 2-tone horn sounds
and the following text appears on the LCD. Interruption

Fig. 5-31 24S: Centrifugation interrupted

48 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Operation

Press the STOP/OPEN button again. The lid


opens and the following text is displayed on the
LCD: Lid is open

Fig. 5-32 24S: lid is open


WARNING
When centrifugation is interrupted by the
operating personnel, the test is invalid. You
must repeat the test using new samples.

5.4.3 Other abnormal procedures

5.4.3.1 Centrifugation interrupted by the centrifuge


Acceleration, vibrations and the nominal speed
are automatically monitored by the centrifuge. If
the centrifuge detects a fault, the started
centrifugation is interrupted automatically and the
centrifuge signals this by the audible and visual
alarms for centrifugation interruption.

The nominal speed was not reached or the


centrifuge head is blocked.

Beethoven melody; the following text appears on Low tacho


the LCD.

Fig. 5-33 24S: Nominal speed not reached

After 3 to 4 seconds, the following message


appears:
Check the head

Fig. 5-34 24S: Check the head

Unbalanced loading of the centrifuge head.

Beethoven melody; the following text appears on


the LCD. Vibrations

Fig. 5-35 24S: Vibrations


After 3 to 4 seconds, the following message
appears:
Check the head

WARNING
Fig. 5-36 24S: Check the head
When centrifugation is interrupted by the
centrifuge, the test is invalid. You must
repeat the test using new samples.

H009120 V13.0-01/2019 49
Operation

5.5 Turning off the centrifuge


If the centrifuge is no longer required for a longer
period of time, e.g. at the end of a working day,
you should switch the centrifuge off properly.

A. Press the STOP/OPEN button; the lid opens.


B. Switch off the power switch at the rear of the
centrifuge.

 The LCD clears.

C. If necessary, clean the centrifuge and the


centrifuge head according to the instructions
section “6.4 Cleaning” on page 54.
D. Place the empty centrifuge head in the centrifuge.
This is the best way to protect it from damage.
E. Close the lid. It is locked automatically.

Fig. 5-37 Placing the centrifuge head back

50 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance 6

Chapter overview

In this chapter all maintenance procedures necessary at regular intervals to maintain operational
readiness and quality assurance are described.

RECOMMENDATION

Most maintenance work can be performed by operating personnel using these instructions.
If necessary, ask a specialist who is authorised to carry out maintenance work.
Observe chapter “2 Safety and handling” as well as all safety instructions contained in this chapter.

DANGER
ELECTRIC SHOCK
If operated or handled incorrectly, electrical equipment can lead to an electric shock. 

Never touch electrical equipment or parts of them, e.g. power cord, power plug or sockets,
with wet hands. Before carrying out any maintenance work switch off the centrifuge and
remove the power cord from the socket at the rear of the centrifuge.

6.1 Maintenance report


RECOMMENDATION

The manufacturer recommends that all maintenance, control and replacement work be entered in a
“Maintenance report”; a copy of which can be found in the appendix of this User Manual.

This report is very useful when searching for the source of malfunctions and should thus be readily
accessible for maintenance personnel.

H009120 V13.0-01/2019 51
Maintenance

6.2 Maintenance plan


The maintenance work described in the maintenance plan below should be carried out by the user or
technical support according to the instructions in the maintenance plan and recorded in the
maintenance report.

The maintenance steps in the maintenance plan are described in the following chapters. Read them
carefully before starting.

WARNING
ID-CENTRI 6S/6S ACCU / ID-CENTRI 24S
No part of the centrifuge may be lubricated or oiled.

ID-CENTRI 12SII
No internal part of the centrifuge may be lubricated or oiled.

Maintenance plan

Time Activity Carried out by Section

As required Cleaning Operator 6.4, p. 54


As required Decontamination Operator/Technical support 6.5, p. 55
Weekly Check the mobility of the ID-Card  Operator/Technical support 6.6.2, p. 56
holders
Weekly Inspect the seal and lid Operator/Technical support p. 56
Monthly 12SII Operator/Technical support 6.6.5, p. 62
Inspect and/or replace O-rings
Every 6 12SII and 24S Operator/Technical support 6.6.6, p. 62
months Inspect and/or replace fan filter
Yearly Check the target speed Technical support 6.6.3, p. 57
Yearly Check the centrifugation time Technical support 6.6.4, p. 59
Yearly 12SII Oil the springs of the lid hinges Technical support

Technical support

Enter the address, telephone and fax numbers of the Bio-Rad technical support below.

Street/No.

Post codeTown

Tel.

Fax

52 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance

6.3 Auxiliary materials


6.3.1 Cleaning agents

The most suitable cleaning agent is a 70% ethyl alcohol solution.

Other agents for cleaning are soft, lint-free cloths and cotton-wool buds or swabs.

Other types of cleaning agents, especially hard objects, are not suitable for use when cleaning.

6.3.2 Measuring devices

6.3.2.1 Stroboscope
You will need an optical speed counter (stroboscope) for checking the accuracy of the target speed.

There is a suitable stroboscope in the manufacturer’s range of products.

6.3.2.2 Stopwatch
You will need a standard commercially available stopwatch for checking the accuracy of the
centrifugation time.

6.3.2.3 ID test cards


Finally, you require a sufficient amount of ID test cards or unused, sealed ID-Cards in order to fill the
centrifuge head completely.

Type Amount of ID test cards

6S / 6S Accu 6
12SII 12
24S 24

The ID test cards can only be obtained from Bio-Rad.

H009120 V13.0-01/2019 53
Maintenance

6.4 Cleaning
The reagents and samples are all contained in the ID-Cards so that it is not necessary for the
centrifuge to come into contact with them. If contamination with samples occurs carry out a
decontamination (see section “6.5 Decontamination” on page 55).

Material required

• mild chloride-free cleaning agent (e.g. 70% ethyl alcohol solution);


• lint-free cloth, cotton buds or swabs.

DANGER
FIRE, EXPLOSION, AND CORROSION
When handling agents, take note of the details on the cleaning agent containers and of all
local safety provisions in force.

CAUTION
Do not use ammonium or chloride-containing cleaning agents or agents which are
abrasive or which crystallise after drying. Never use hard objects.

When using cleaning agents, make sure you read the instructions on the
container and adhere to all statutory safety regulations.

A. Switch off the centrifuge with lid open and remove the power cord.
B. Remove the centrifuge head from the centrifuge.
C. Clean the tub, lid and outer housing with a lint-free cloth moistened with a chloride-free cleaning
agent.
D. Clean the surfaces of the centrifuge head in the same way. Clean the inside of the card holders using
cotton buds or swabs moistened with a chloride-free cleaning agent.

RECOMMENDATION

Once cleaning has been completed, give the centrifuge and the head sufficient time to dry (at least
30 minutes).

DANGER
FIRE AND EXPLOSION
If the time allowed for drying is too short, the fumes from the cleaning agents are unable
to escape. These fumes can ignite when the centrifuge is turned on.

E. Place the power cord back into position

 You can bring the centrifuge back into service.

54 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance

6.5 Decontamination
The reagents and samples are enclosed in the ID-Cards if used correctly so that they do not come
into contact with the centrifuge, centrifuge head or card holder. However, if contamination of the
centrifuge, centrifuge head or card holder occurs, they must be decontaminated immediately.

RECOMMENDATION

Do not use a cleaning agent which contains chloride or ammonium, or one which crystallises after
drying, for decontamination purposes, as this may damage the centrifuge. Consult the manufacturer
if you intend to use any cleaning agent not listed in this User Manual, his approval should be
obtained.

DANGER
RISK OF INFECTION
If the centrifuge, centrifuge head or card holder is contaminated, there is a risk of
infection. Observe all safety regulations which apply and carry out a decontamination. 
Wear protective gloves. 
All material used in the decontamination procedure, e.g. cloth, cotton buds, swabs, should
be considered contaminated and thus disposed of. 
In the case of extreme contamination it may be necessary to disassemble centrifuge
components, e.g. the centrifuge head. Consult a specialist in this case.

WARNING
When carrying out decontamination, wear appropriate protective equipment, at least
gloves.

H009120 V13.0-01/2019 55
Maintenance

6.6 Check and test of the instrument


6.6.1 Seal and lid

RECOMMENDATION

If any damage is found the seal must be replaced. Consult the Bio-Rad technical support team. In
the case of damage switch off the centrifuge. Mark the centrifuge as defective to prevent further
use.

The seal and the lid are safety components. They


prevent the area around the centrifuge from being
contaminated with the sample material. It is
therefore essential that these parts are inspected
periodically.

A. Check the seal for damage, e.g. nicks, tears and


pressure marks.
B. Check the lid, hinges and locking bolts for
damage.

Fig. 6-1 Points to check on the centrifuge

6.6.2 Mobility of the ID-Cards holders

The ID-Cards holders on the centrifuge head are


strongly stressed due to the forces occurring.
The ID-Cards holders must therefore be checked
for ease of movement and the full 90 degree
swivel angle.

A. Place the centrifuge head in the centrifuge.


B. Then swivel each ID-Cards holder 90 degrees.
Let go of the ID-Cards holder. It must fall back
into it’s normal position through its own weight.
If one or several ID-Cards holders do not move
to the full 90 degree swivel angle or if the swivel
movement is stiff, do not use this centrifuge head
any more for tests. Contact a specialist.

C. Enter any finding in the maintenance report.

Fig. 6-2 Holder inclination


NOTE
The ID-Cards holders are numbered on the
centrifuge head in order to ensure that you do
not leave any out.

56 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance

6.6.3 Checking the target speed

Before commencing operations, the accuracy of the speed and the function of the centrifuge ID-Cards
holder should be checked. The constancy of the speed and the pivoting of the ID-Cards holder to
90 degrees are decisive factors in determining the quality of the centrifugation carried out.

6.6.3.1 Equipment required


• Stroboscope
• ID test cards (or unused and sealed ID-Cards)

RECOMMENDATION

Specialist knowledge in handling a stroboscope is required if the speed is to be checked


successfully. If you are not in possession of such knowledge, please contact your Bio-Rad
representative.

6.6.3.2 Procedure
A. Turn on the centrifuge using the power switch at the rear.
B. Press the STOP/OPEN button.The lid opens.
C. Fill the centrifuge head with the required number of ID test cards or ID-Cards for the first test of a
series of three tests. Read the section “5.1.1.2 Loading the centrifuge head” on page 38.

Test 1 Test 2 Test 3


Centrifuge type
No. of ID-Cards No. of ID-Cards No. of ID-Cards

6S / 6S Accu 2 4 6

12SII 4 8 12

24S 8 16 24

D. Close the lid until it engages audibly.


E. Place the switched-on stroboscope precisely
above the card holders on the lid.
F. Press the START button.

 The centrifuge starts.

G. When the centrifuge has stabilized to nominal


speed, adjust the stroboscope until the head
visually stands still.

Fig. 6-3 Stroboscope position

H009120 V13.0-01/2019 57
Maintenance

6S / 6S Accu
The nominal speed of 1175 rpm is reached when
the READY LED starts to flash.

Fig. 6-4 6S / 6S Accu centrifuge

12SII
The nominal speed of 1030 rpm is reached when
the timer starts counting backwards. 1030 rpm 9:59

Fig. 6-5 12SII centrifuge

24S
The nominal speed of 910 rpm is reached when
the timer starts counting backwards. 910 rpm 9:59
H. Compare the rpm displayed on the stroboscope
with the nominal speed. The permitted deviation
for all centrifuges is max. ± 5 rpm. Write down Fig. 6-6 24S centrifuge
the reached speed.
I. On the 12SII and 24S, check that the speed
displayed on the centrifuge coincides.
J. Check through the transparent lid whether all
card holders have completely swiveled to 90°.
K. Press the STOP/OPEN button.
L. Perform tests 2 and 3 using the prescribed 90
number of ID test cards or ID-Cards.
M. Open the maintenance report as described in the
section “8.2.2 Maintenance record” on page 72
and enter the test results of all three tests in the
maintenance report.
Fig. 6-7 ID-Cards swivel

CAUTION
If any of the tests is negative, immediately
inform your Bio-Rad agent. Do not perform
any centrifugations with this centrifuge.

58 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance

6.6.4 Checking the centrifugation time

Before commencing operations, the accuracy of the centrifugation time of the centrifuge should be
checked. Compliance with the precise centrifugation time is a decisive factor in determining the quality
of the centrifugation carried out.

6.6.4.1 Equipment required


• Stopwatch
• ID test cards (or unused and sealed ID-Cards)

6.6.4.2 Procedure
A. Switch on the centrifuge using the power switch at the rear.
B. Press the STOP/OPEN button. The lid opens.
C. Fill the centrifuge head with the required number of ID test cards or ID-Cards for the first test of a
series of three tests. Read the section “5.1.1.2 Loading the centrifuge head” on page 38.

Test 1 Test 2 Test 3


Centrifuge type
No. of ID-Cards No. of ID-Cards No. of ID-Cards

6S / 6S Accu 2 4 6

12SII 4 8 12

24S 8 16 24

D. Close the lid until it engages audibly.


E. Press the START button. The centrifuge starts
rotating.
F. When the centrifuge has stabilized to nominal
speed, start the stop-watch.

 Start of centrifugation time:

6S / 6S Accu
The centrifugation time starts when the READY
LED starts to flash. During the centrifuge time of
10 min, the READY LED flashes slowly for 8
minutes and then flashes quickly for the last 2
minutes.

Fig. 6-8 6S / 6S Accu centrifuge

12SII and 24S


The centrifugation time starts when the timer
starts counting backwards. 1030 rpm 9:59

Fig. 6-9 12SII centrifuge

H009120 V13.0-01/2019 59
Maintenance

G. When the end of the centrifugation is reached,


stop the stop-watch. 910 rpm 9:59

 End of centrifugation time:


Fig. 6-10 24S centrifuge

6S / 6S Accu
At the end of the centrifugation time the READY
LED extinguishes.

Then the 2-tone horn sounds, the lid opens.

Fig. 6-11 6S / 6S Accu centrifuge

12SII
At the end of the centrifugation time the
message in the LCD turns to „STOP“. Then the Wait please
following messages appear in the LCD and the
2-tone horn sounds:

Fig. 6-12 12SII centrifuge

Process finished

Fig. 6-13 12SII centrifuge

After 3 to 4 seconds, the lid opens and the


following message appears in the LCD:
Lid is open

Fig. 6-14 12SII centrifuge

24S
At the end of the centrifugation time the following
messages appear in the LCD: Open lid

Fig. 6-15 24S centrifuge

60 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Maintenance

The 2-tone horn sounds and the lid remains


closed.

H. Check the reached centrifugation time against the Process finished


nominal time. The permitted deviation for all
centrifuges is max. ± 12Sec. Note down the
centrifugation time reading. Fig. 6-16 24S centrifuge

I. Then perform tests 2 and 3 using the


corresponding number of ID test cards or ID-
Cards.
J. Open the maintenance report as described in the
section “8.2.2 Maintenance record” on page 72
and enter the test results of all three tests in the
maintenance report.

CAUTION
If the test is negative, inform your Bio-Rad
representative immediately. Do not carry out
any centrifugation with this centrifuge.

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Maintenance

6.6.5 12SII Centrifuge: Inspecting the O-rings

The centrifuge 12SII has a tapered drive spindle


with 2 O-rings as drivers. The O-rings are made
of rubber and have a restricted life.

CAUTION
Replacing the O-rings is a task reserved to
duly trained technical personnel.

Fig. 6-17 O-rings location

6.6.6 12SII and 24S Centrifuges: Fan Filter

6.6.6.1 Inspecting the Fan Filter


The centrifuges 12S ll and 24S have an
integrated fan to cool the centrifuge to normal
temperature during operation. The intake air is
cleaned by a fan filter. The fan filter must be
inspected every 6 months. If it is contaminated, it
must be replaced.

6.6.6.2 Replacing the Fan Filter


Material required

• screwdriver size 2
• fan filter (Part No. 027 12 12)

Procedure

A. Switch off the centrifuge using the power switch.


B. Disconnect the power plug.

NOTE
12SII Centrifuge: The fan filter is on the bottom.
Turn the centrifuge on one side. Make sure the
centrifuge head has been removed. Fig. 6-18 Replacing the fan filter

C. Use the screwdriver to remove the plastic cover of


the fan filter.
D. Check the fan filter (contrast between bright and
light areas on the filter). If the contrast is severe,
replace it with a new fan filter.
E. Make sure you insert the new fan filter with the
smooth side facing outwards.
F. Put the fan filter cover back into place.

62 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Troubleshooting 7

Chapter overview

Your ID-Centrifuge should function smoothly for many years if used properly and subjected to regular
maintenance. However, malfunctions may occur occasionally. In this chapter the possible malfunctions
and the remedies necessary are described.

RECOMMENDATION

Most remedies can be carried out by the operator according to the following instructions.
Do not carry out instructions intended only for authorised specialists.
Observe chapter “2 Safety and handling” as well as all safety instructions contained in this chapter.

DANGER
ELECTRIC SHOCK
If operated or handled incorrectly, electrical equipment can lead to an electric shock. 
Never open the centrifuge housing in the case of a malfunction. Only authorised persons
may do this.

7.1 List of possible problems


CAUTION
The user must have read and understood this documentation before carrying out
any troubleshooting whatsoever with the ID-Centrifuge 6S / 6S Accu, ID-
Centrifuge 12SII, or ID-Centrifuge 24S. In case of unclear information, please
contact the distributor.

RECOMMENDATION

If it is not possible to remedy a malfunction, contact the Bio-Rad customer service.

H009120 V13.0-01/2019 63
Troubleshooting

7.1.1 Centrifuge 6S / 6S Accu

POWER LED remains dark


- Power cord not plugged in

 Plug in the power cord.

- Power switch turned off

 Turn on the power switch.

- The socket which the centrifuge is plugged into is not providing an electricity supply

 Try plugging the centrifuge into another socket.

- Power fuse defective

 Call technical support.

READY LED remains dark


- Cover lock not engaged

 Close lid again until lock engages audibly.

ALARM LED comes on


- Centrifuge head blocked

 Insert centrifuge head correctly.

Cover does not open


- Seal sticking

 Clean lid and seal.

- Controller fault

 Switch off power switch and switch on again.

 Press STOP/OPEN button.

Cover still does not open


- Lock defective

 Call technical support.

- Controller fault

 Call technical support.

64 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Troubleshooting

Incorrect speed
- Controller fault

 Call technical support.

Incorrect centrifugation time


- Controller fault

 Call technical support.

H009120 V13.0-01/2019 65
Troubleshooting

7.1.2 Centrifuges 12SII and 24S

Back light on LCD dark, no text message


- Power cord not plugged in

 Plug in the power cord.

- Power switch turned off

 Turn on the power switch.

- The socket which the centrifuge is plugged into is not providing an electricity supply

 Try plugging the centrifuge into another socket.

- Power fuse defective

 Call technical support.

«Vibrations/check head»
- Centrifuge head loaded unbalanced

 Load centrifuge head as per section “5.3.1.2 Loading the centrifuge head” on
page 43 or section “5.4.1.2 Loading the centrifuge head” on page 47.

«Cover open»
- Cover lock not engaged

 Close lid again until lock engages audibly.

«Insert head»
- Centrifuge head not inserted

 Insert centrifuge head.

«Check head and low tacho»


- Centrifuge head blocked

 Insert centrifuge head correctly.

12SII ONLY 
«High tacho»
- Defective O-rings on drive spindle

 Replace O-rings as per section “6.6.5 12SII Centrifuge: Inspecting the O-


rings” on page 62.

66 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Troubleshooting

Cover does not open


- Seal sticking

 Clean lid and seal.

- Controller fault

 Switch off power switch and switch on again.

 Press STOP/OPEN button.

Cover still does not open


- Lock defective

 Call technical support.

- Controller fault

 Call technical support.

Incorrect speed
- Controller fault

 Call technical support.

Incorrect centrifugation time


- Controller fault

 Call technical support.

H009120 V13.0-01/2019 67
Troubleshooting

7.2 Other operations


7.2.1 Opening the lid mechanically

7.2.1.1 ID-Centri 6S / 6S Accu and ID-Centri 12SII


(1)
By opening mechanically the centrifuge, samples
can be removed after a power cut or if the
(2)
electronics or mechanism is defective.

A. Remove the plastic lid (1). (3)


B. Using an hexagonal socket wrench (2), remove
the screw (3).

Fig. 7-1 Opening the centrifuge (6S / 6S Accu and 12SII)

7.2.1.2 ID-Centri 24S


A. Insert a screwdriver in the small hole in the right-
hand side of the centrifuge.

Fig. 7-2 Opening the centrifuge (24S)

68 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Appendix 8

8.1 Graphical symbols


8.1.1 Instrument-related symbols

Symbol Description

Biohazard

The sticker on top of the housing has the following meaning:


Equipment can be potentially infectious due to the samples or reagents used.

Disposal of Waste Equipment


This symbol on our product nameplates indicates that this product must not be dis-
posed of with your other waste. You must dispose of this product by handing it
over to a designated collection point for the recycling of electrical and electronic
equipment waste. Contact your local city office or your waste disposal service for
more information on the collection.

8.1.2 Package-related symbols

Symbol Description

Always carry upright.

Do not throw nor let the instrument fall down.

Do not use a handle to carry.

Protect from moisture and water sprays.

Do not stack more than xx (12 in the example beside) identical packings on the first
one.
12

H009120 V13.0-01/2019 69
Appendix

8.2 Quality system


8.2.1 Decontamination certificate

The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S and their accessories must
be cleaned and decontaminated before performing any maintenance, revision task, before taking the
instrument out of service and when preparing for transportation.

 See section “6 Maintenance” on page 51.

NOTE
You can find the official decontamination certificate on the Bio-Rad website, section «distributors -
secret area - official forms».

WARNING
Fill in a copy of the decontamination certificate and place it on the ID-Centrifuge 6S / 6S
Accu, ID-Centrifuge 12SII, or ID-Centrifuge 24S, in a conspicuous place.

The absence of decontamination certificate may extend the time needed for revision and maintenance.

Bio-Rad will bill any cleaning and decontamination operation.

RECOMMENDATION

The original certificate must remain in the present manual.


When a certificate is required, make a copy and fill in the latter sample.

70 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Appendix

   
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H009120 V13.0-01/2019
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Appendix

8.2.2 Maintenance record

RECOMMENDATION

The original blank maintenance sheet must remain in the present manual.
When a maintenance record is required, make a copy and fill in the latter sample.

Type Page

Serial number 

First startup

Name: Date: Signature:

Speed in rpm Test 1 Test 2 Test 3

Sould be ±5 rpm rpm rpm rpm

Time in min Test 1 Test 2 Test 3

Sould be 10 min
±12 sec min min min

Maintenance / Event / Defect Action Operator Date

72 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Appendix

Type Page

Serial number 

Maintenance / Event / Defect Action Operator Date

H009120 V13.0-01/2019 73
Appendix

8.2.3 Performance variation

If any variation of the performance of the instrument is observed or suspected, contact immediately
your Bio-Rad representative.

8.3  Conformity
The ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S have been designed,
constructed and fabricated in compliance with the following guidelines and standards:

• 98/79/EC;
• 73/23/EEC;
• EN 61326:1997 + A1:1998 + A2:2001;
• EN 61000-3-2:2000;
• EN 61000-3-3:1995 + A1:2001;
• EN 61010-1:2001;
• EN 61010-2-101:2002;
• EN 61010-2-020:1992 + A1:1996.

The EU declaration of conformity which applies is supplied with the centrifuge.

74 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Appendix

8.4 Device disposal


8.4.1 General warnings

WARNING
In order to protect the persons and the environment, any instrument and its accessories
must be disposed of in an appropriate way. Its is mandatory to strictly apply laws and local
bylaws relative to an appropriate disposal procedure.

CAUTION
An instrument may only be dismounted and divided into its basic components by a
qualified technician.
Parts and sub-parts removed by a technician may not be reused unless their
intended use has been approved in writing by the manufacturer. Any future
application has to be precisely described in the request.

8.4.2 Disposal

The following components may be found in the instruments:

• packing material: cardboard, LD-PE (padding);


• electrical components: e.g. electric motor, solenoid, plastic-insulated wires;
• electronic components: e.g. epoxy printed circuit boards, electronic components;
• plastics: e.g. LD-PE, PUR (housing), Plexiglas (cover), silicon (cover seal), POM;
• metals: e.g. aluminium, sheet metal, copper.

8.5 List of documents


8.5.1 User Manual

English.................................................................H009120
German ...............................................................H009069
Italian ...................................................................H009070
Spannish .............................................................H009071
Portuguese ..........................................................H009072
Greek ..................................................................H009073
Swedish...............................................................H009074
Danish .................................................................H009075
French .................................................................H009076
Norwegian ...........................................................H009113
Croatian...............................................................H009109

H009120 V13.0-01/2019 75
Appendix

8.5.2 Service Manual (english only)

Centrifuge 6S / 6S Accu ...................................H005218


Centrifuge 12SII ..................................................H005266
Centrifuge 24S ....................................................H005269

8.6 Identification plate


(1) (2) (3)
A model identification plate has been attached to
the back of the ID-Centrifuge in order to identify
your ID-Centrifuge exactly. This plate contains the
following information:

(1) Type
(2) Serial number
(3) Item number
(4) Nominal operating speed
(5) Power supply connection
(voltage, frequency, power consumption)
(6) Fuse for 100–240 VAC (4) (5) (6) (7) (8)
(7) IVD In vitro diagnostic medical device
Fig. 8-1 Identification plate
(8) Date of manufacture (year + month)
Please copy the following information from the
identification plate in the fields below:

Type

Serial number

76 ID-Centrifuge 6S / 6S Accu, ID-Centrifuge 12SII, and ID-Centrifuge 24S User Manual • © Bio-Rad
Notes:

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Website www.bio-rad.com/diagnostics Australia 61-2-9914-2800 Austria 43-1-877-8901 Belgium +32 (3)710-53-00 Brazil +55 (31)3689-6600 Canada 1-514-334-4372
Clinical China 86-21-61698500 Czech Republic 420-241-430-532 Denmark +45-4452-1000 Finland 358-9-804-22-00 France 33-1-47-95-60-00 Germany +49 (0)89-318-840
Greece 30-210-7774396 Hong Kong 852-2789-3300 Hungary +36-1-459-6100 India 1800-180-1224 Israel 972-3-9636050 Italy +39-02-216091 Japan 81-3-6361-7070
Diagnostics Group Korea 82-2-3473-4460 Mexico +52 (55)5488-7670 The Netherlands +31-318-540666 New Zealand 64-9-415-2280 Norway +47-23-38-41-30 Poland 48-22-3319999
Portugal 351-21-472-7700 Russia +7-495-721-1404 Singapore 65-6415-3170 South Africa 27-11-442-85-08 Spain 34-91-590-5200 Sweden +46-8-555-127-00
Switzerland +41 (0)26-674-55-05/06 Taiwan 886-2-2578-7189 Thailand 662-651-8311 United Kingdom +44 (0)20-8328-2000

User Manual H009120 V13.0 © Bio-Rad 01/2019

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