Prominence-I Maintenance Guide EN

228-91532
Mar. 2014
SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH
Prominence-i
LC-2030
LC-2030C
LC-2030C 3D
Maintenance Guide
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction
Read this Instruction Manual thoroughly before using
the product.
Thank you for purchasing this product.
This manual describes the maintenance for this product, including inspection, part
replacement, and rinsing. Read this manual thoroughly before using the product and
operate the product in accordance with the instructions in this manual. The following
instruction manuals are included with the product. PDF documents are included in the
instruction manual CD-ROM(Part No. S228-56248-41).
System Guide Describes the basic operation and troubleshooting for this
(Booklet/PDF) product.
Describes "validation" for verifying that this product meets
Integrity Guide "CHECK CRITERIA" and "calibration" for adjustment so that this
(PDF) product meets the CHECK CRITERIA, in order to secure the
reliability of analysis data.
Describes the operation of the operations screen and the
Operation Guide
operational method for the Web screen, iPhone, and iPad for
(PDF)
this product.
Maintenance Guide This instruction manual. Describes the maintenance for this
(PDF) product, including inspection, part replacement, and rinsing.
Read "Introduction" in this section thoroughly before using the product. "Introduction"
describes the information about the warranty, after-sales service, safety instructions and
precautions to ensure safe operation of the instrument.
For information on the operation, hardware validation, and accessories and options for
this product, refer to "System Guide", "Integrity Guide", and "Operation Guide".
Keep this manual for future reference.
Important
• If the user or usage location changes, ensure that this manual is always kept
together with the product.
• If this manual or a product warning label is lost or damaged, immediately contact
your Shimadzu representative to request a replacement.
• To ensure safe operation, read "Safety Instructions" P.iv thoroughly before using
the product.
• To ensure safe operation, contact your Shimadzu representative if product
installation, adjustment, re-installation (after the product is moved), or repair is
required.
ã 2013 Shimadzu Corporation. All rights reserved.

Original version is approved in English.
Prominence-i LC-2030 LC-2030C LC-2030C 3D i

Introduction
Notice
• Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
• Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
• All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission in writing from Shimadzu Corporation.
• A part of illustration of this manual might be different from the instrument used.
• Microsoft and Windows are registered trademarks of Microsoft Corporation in the
United States and/or other countries.
iPhone and iPad are registered trademarks of Apple Inc. in the United States and/or
other countries.
Other company names and product names mentioned in this manual are trademarks or
registered trademarks of their respective companies. The ä and â symbols are omitted
in this manual.
• Prominence-i is registered trademarks of Shimadzu Corporation in the United States and
Europe (Office for Harmonization in the Internal Market).
ii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Indications Used in This Manual
Indications Used in This Manual

Warnings, cautions, and notes are indicated using the following conventions:
Indication Meaning
Indicates a potentially hazardous situation which, if not

avoided, could result in serious injury or possibly death.
Indicates a potentially hazardous situation which, if not

avoided, may result in minor to moderate injury or equipment
damage.
Emphasizes additional information that is provided to ensure
the proper use of this product.
The following symbols are used in this manual:
Indication Meaning
Indicates an action that must not be performed.
Indicates an action that must be performed.
Indicates information provided to improve product performance.
Indicates the location of related reference information.
Prominence-i LC-2030 LC-2030C LC-2030C 3D iii

Introduction
Safety Instructions
To ensure safe product operation, read these important safety instructions carefully
before use and follow all WARNING and CAUTION instructions given in this section.
n Product Applications
Use this instrument ONLY for the intended purpose.

This instrument is a high performance liquid chromatograph system. Using this
instrument for any other purpose could cause accidents.
Safety regulations and standards

For notifications on installation and safety controls, follow the necessary
procedures in compliance with the laws and regulations applicable in the
country where the product is used.
n Installation Site
Use of open flame in the vicinity of this instrument

must be strictly prohibited.
Do NOT install the instrument in the same room
with any other equipment that emits. Otherwise
could potentially emit sparks, since sparks could
cause a fire.
High performance liquid chromatograph uses large
amounts of flammable organic solvents.
Provide fire extinguishers for use in case of fire.
The room where the instrument is installed should

be well ventilated.
Otherwise, solvent vapors could cause poisoning or
ignite and cause a fire. The solvents used in high
performance liquid chromatograph are flammable
and toxic.
Provide sink washing equipment as close to the

instrument as possible.
If solvent gets into the eyes or onto the skin, it must
be flushed away immediately.
iv Prominence-i LC-2030 LC-2030C LC-2030C 3D

Safety Instructions
Avoid installation sites that are exposed to corrosive gases or excessive dust.
These adverse conditions may be detrimental in maintaining the instrument's
performance and may shorten its service life.
Keep away from equipment generating strong magnetic fields.

Do NOT install the instrument near equipment that generates strong magnetic
fields. If the power supply line is subject to high electrical noise, install a surge
protector.
When transporting the instrument, do not hold it by the front panel.

Otherwise, it may cause the front panel to get damaged or detached.
During installation, consider the entire mass of this system combined with
other components.
• Model without a sample cooler: 58 kg
• Model with a sample cooler: 63 kg
The mass of this system is as described above. Please set up this instrument in
consideration of the entire mass combined with other components, PC, the
monitor, and the printer, etc.
"7 Technical Information" in the System Guide
The lab table on which this instrument is installed

should be strong enough to support the total mass
of the system. It should be leveled, stable and have
depth of at least 600 mm.
Otherwise, the instrument could tip over or fall off
the table.
Keep at least 100 mm between the rear of the

instrument and the wall.
This allows for sufficient air circulation and
ventilation from the grille to provide cooling and
prevent the instrument from overheating and
impairing the performance.
Install the instrument in a location that satisfies the following conditions to

preserve its performance:
• The room temperature is maintained between 4 and 35 °C, with minimal
temperature variation during a day.
• Air currents from heating or air conditioning equipment are not directed
onto the instrument.
• Ensure that Instrument is not exposed to direct sunlight.
• There is no vibration.
• Humidity is maintained within 20 to 85 %.
• There is no condensation.
• The location conforming to the installation environment (IEC)
(Installation category II, Pollution degree: 2, Altitude: Up to 2000 m, Indoor)
Prominence-i LC-2030 LC-2030C LC-2030C 3D v

Introduction
Install the product in locations where the power switch can be easily operated.
The power switch must be turned off in case of an emergency.
n Installation
To ensure safe operation, contact your Shimadzu representative if product installation,

adjustment, or re-installation (after the product is moved) is required.
Do NOT place heavy objects on the power cord. Do

NOT keep any hot items in the vicinity.
Do NOT modify the cord in any way. Do NOT bend
it excessively.
Otherwise, fire, electric shock or malfunction may
occur.
If the cord is damaged, contact your Shimadzu
representative.
Use only the specified cable.

Arrange the cables as specified.
occur.
Do NOT insert the adapter's grounding lead into

the power outlet or allow it to make contact with
the power outlet.
Otherwise, fire or electric shock may occur.
Do NOT connect the grounding lead to the gas

tube, the water service tube or the telephone line.
Otherwise, accidents or malfunction may occur.
To unplug the instrument, pull the plug itself, NOT

the cord.
Before connecting the cable, turn off the power and
unplug the instrument.
occur. If the cord is damaged, contact your
Shimadzu representative.
Take measures to prevent the instrument from falling in the event of an

earthquake or other disaster.
Strong vibrations could cause the instrument to fall over, resulting in injury.
vi Prominence-i LC-2030 LC-2030C LC-2030C 3D

Safety Instructions
Ground the instrument.

Grounding is necessary to prevent electric shock in the event of an accident or
electrical discharge, and important for ensuring stable operation.
To prevent electric shock and to maintain stability in operation of the product,
be sure to ground the product.
The product will be grounded when the provided 3-wired power plug is
inserted into a 3-wired power socket equipped with a ground terminal.
Connect the instrument only to a power supply of indicated voltage in

consideration of total power consumption of this instrument, other LC
components, PC, monitor, and printer, etc.
Otherwise, fire or electric shock could result.
Check that the power supply voltage is stable and that its current capacity is
sufficient to operate all the components of the system. If not, the instrument
will not operate at its rated performance, affecting not only this instrument,
but other instruments connected to the same power supply. Connect the
instrument to a power supply that complies with the capacity and use a power
cord that complies with the capacity.
Please refer to the following for power supply voltage and power consumption
of this product.
"7 Technical Information" in the System Guide
When installing the instrument, be careful not to

pinch your fingers between the system components.
Otherwise, injury may result from pinching your
fingers, because the clearance between the
components is only 20 mm.
When opening the doors, be careful not to pinch

your fingers as this could result in injury.
Before plugging in the instrument, make sure that

the main power switch on the side of the
instrument is OFF.
Prominence-i LC-2030 LC-2030C LC-2030C 3D vii

Introduction
n Operation
Do NOT use flammable sprays (hair sprays,

insecticide sprays, etc.) near the instrument.
Otherwise, they could ignite and cause a fire.
Never use a cracked reservoir bottle.

If a helium degassing unit is used, pressure is
exerted on the reservoir bottles and may cause
cracks in the bottles. It could break the reservoir
bottles and cause injury.
Do NOT apply water to office equipment such as the PC as well as the

instrument.
Before removing any high pressure flow line part, such as tubing, the sample
loop, the needle, and the needle seal, follow the safety precautions below:Be
sure to stop the pump and confirm the pressure of the mobile phase has fallen
to zero.
Removing any of these parts while high pressure remains could cause the
mobile phase and the clogging particles to eject rapidly and injury could occur.
Confirm that the pressure has fallen to zero before removing these parts.
If the pressure has not fallen to zero, the high-pressure flow line might be
clogged. After confirming the end of the drain tubing is in the waste container,
open the drain valve and release the pressure of the mobile phase.
Take thorough measures to prevent buildup of

static electricity.
Static electricity could result in fires or explosion.
"Static Electricity Precautions" P.xi
Always wear protective gloves and protective

goggles when handling solvents and samples.
If solvent gets into the eyes, it can result in
blindness.
Should solvent get into the eyes, flush immediately
with large amounts of water and get medical
attention.
Do NOT bend any tubing of the instrument at the same position repeatedly.
Otherwise, a rupture or cracks of the tubing are likely to occur, and might
result in solvent leaks.
viii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Safety Instructions
n Inspection and Maintenance
Never remove the main cover.

Otherwise, this may cause injury or malfunctioning of the instrument.
The main cover does not need to be removed for routine maintenance,
inspection and adjustment. Have your Shimadzu representative perform any
repairs requiring removal of the main cover.
Do NOT allow spilled water to remain on the instrument surface, and do not
use alcohol or thinner-type solvents to clean the surfaces.
Otherwise, these can cause rusting and discoloration.
If the power cord plug gets dusty, remove the plug from the power outlet
and wipe away the dust with a dry cloth.
Otherwise, fire may occur.
Replacement parts must be of the specifications given in "Component Parts"

or "Maintenance Parts" of the instruction manuals such as the System Guide
and the Maintenance Guide.
Use of any other parts may result in instrument damage and malfunction.
Dispose of the waste liquid properly and in accordance with the instruction
by your administrative department.
n Repair, Disassembly and Modification
Do NOT modify or disassemble the product without permission.

Otherwise, electric shock or short-circuit accidents may occur. This may also
cause injury or malfunctioning of the instrument.
When repair is necessary, request through your Shimadzu representative.

Failing to do so may result in an ignition, electric shock, or injury.
Prominence-i LC-2030 LC-2030C LC-2030C 3D ix

Introduction
n In an Emergency
If any problem is detected, such as a burning smell, take the following action:
Emergency Shutdown Procedure
1 Turn the power to the instrument OFF.
2 Disconnect the power cable at the rear of the instrument.
When the instrument is used again, inspect the instrument and, if necessary, contact your
Shimadzu representative to request servicing.
n During a Power Outage
Take the following measures in the event of a power outage.
1 Turn the power to the instrument OFF.
2 After confirming all related items in this section "Installation" P.vi and "Operation"
P.viii, use the standard startup procedure to start the instrument.
x Prominence-i LC-2030 LC-2030C LC-2030C 3D

Static Electricity Precautions

Liquid chromatograph (LC) uses flammable organic solvent(s) as the mobile phase. LC
systems are also often used where large amount of flammable substances are present.
Thus, an accident can produce large scale damage. Operators must be constantly on guard
against accidents involving fire or explosion.
The major cause of these accidents is static electricity. Devising preventative measures for
static electricity can be difficult, because the symptoms before an accident vary and can
be hard to detect, since such accidents occur as a result of several simultaneous incidents.
Recommended methods for preventing static electricity accidents are provided below.
Take thorough safety measures based on this information.
n Typical Cause of Static Electricity Accidents
Static electricity accidents are generally caused by this sequence of events:
Generation of static When liquid is passed through thin tubing at high flow
electricity rates, as in liquid chromatograph, the electrostatic charges
of the flowing matter generate static electricity.
Accumulation of static If electrostatically charged liquid is allowed to accumulate

electricity in an electrically insulated container, the charge will
gradually increase, and can eventually reach several
È thousand volts.
Release of energy through If this happens and an electrical conductor is brought
electrical discharge within a certain distance of the container, an electrical
discharge will occur, releasing thermal energy which will
È
ignite any flammable gas of sufficient density in the
Ignition of flammable vicinity.
substances
Prominence-i LC-2030 LC-2030C LC-2030C 3D xi

Introduction
Preventing Static Electricity Accidents
The best way to prevent static electricity accidents is simply to prevent the occurrence
and accumulation of electrostatic charges.
It is important to take multiple preventive measures simultaneously.

If large amounts of flammable solvents are collected in a large container,
implement preventative measures 1, 2, and 3 below.
Keep the room at a proper humidity.

Ambient humidity exceeding 65 % will prevent static.
n Preventive Measure 1
Use a metal container for the waste liquid, and ground the container.
This will ensure that the electrical charges of the container and liquid pass to the ground.
Accessories for this measure
(1) Grounding wire with clip Part No. S228-21353-91

(2) 18-L metal container Part No. S038-00044
(3) 4-L metal container Part No. S038-00043-01
Be sure to ground the metal waste container properly.

If the grounding wire is not properly attached or connected to the ground,
static electricity can build up in the container.
Be sure to verify, using a tester, that the waste container is properly grounded.
Some metal containers have surfaces that are oxidized or laminated. Such a
container may not conduct electricity.
If the liquid to be drained into the waste container is virtually nonconductive

(10-10 S/m or less), it will be necessary to add properly conductive, and
therefore safe, liquid to the tank.
This conductive liquid may be added beforehand.
xii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Cover the spaces between the tubing and the sides of the inlet and outlet openings of
the waste container with caps or other protective covering. This will prevent any sparks
generated outside the container from getting inside.
Accessories for this measure

Caps for 18-L or 4-L container (with three 3-mm diameter openings)
Part No. S228-21354-91
Keep electrostatically charged objects, including the human body, away from the waste
liquid container.
To prevent electrostatic charging of the human body, take the following precautions:
• Wear anti-static clothing and shoes.
• Ground the human body with anti-static wrist straps. (For safety, the wrist strap should
be connected to the ground using an intervening resistor of about 1 MW.)
• Spread anti-static matting or the like on the floor, to make the floor conductive.
Persons who have not taken anti-static precautions should touch some
grounded metal object before coming near the waste liquid container, in order
to drain static charges.
Use tubing with an inner diameter of at least 2 mm for drain lines with high flow rates.
Periodically check the tubing connections for leaks.

Air bubbles in liquid can multiply the electrostatic charge by a factor of 20,
30 or more.
Prominence-i LC-2030 LC-2030C LC-2030C 3D xiii

Introduction
If it is not possible to use a conductive waste liquid container, take the following
precautions:
Ensure that the end of the inflow tubing is always submerged inside the container. Also,
place some type of grounded metal object, such as a ground wire connected to the
instrument, into the liquid.
For low conductivity (less than 10-10 S/m) liquids, take preventive measures 1
to 4.
For low conductivity (less than 10-10 S/m) liquids, preventive measure 5 has no
effect.
Use as small a container as possible to minimize damage in the event of fire.
For Reference
Anti-static equipment (anti-static clothing, shoes and matting) and charge measurement
equipment (potentiometer) are sold by specialty manufacturers.
xiv Prominence-i LC-2030 LC-2030C LC-2030C 3D

Precautions for Mobile Phase Selection and Use
Precautions for Mobile Phase Selection and Use
Do NOT use resin parts for the high-pressure tubing while pumping at high
pressures.
Pumping at high pressure may cause resin tubing to be ruptured or
disconnected, which could result in mobile phase leaks.
Please note the maximum withstand pressure of each part when resin parts
are used for the high-pressure tubing.
Do NOT use highly volatile acids, such as acetic acids in high concentration
(10 % to 50 %) or 1 % TFA (trifluoroacetic acid) solution, as a mobile phase
or rinse solution of the autosampler continually.
Doing so could cause metallic parts in the instrument to corrode. If such liquids
have been used for analysis, purge the mobile phase or rinse solution from the
flow line with distilled water or other liquid that is less corrosive. Also, turn
the autosampler off after analysis and open the front door slightly to let the
vapor release from the inside of the instrument.
Do not use solutions of pH 13 or more..

Some types of mobile phases may damage the flow cell quartz if used for a
long period of time at pH10 or more, resulting in transformation of the
transmission characteristics. After using this type of mobile phase, pump HPLC
grade pure water or other liquid to rinse the flow cell.
The following solvent could damage the system. Never use it in the degassing
unit.
• HFIP (Hexafluoroisopropanol) • Perfluoro 1-methyldecalin

• HF (Hydrogen fluoride) • Perfluoro dimethyldecalin
• Freon 113 • Perfluoro methyl-cyclohexane
• Fluorinert FC-40 • Perfluoro dimethyl-cyclohexane
• Fluorinert FC-72 • AK-225
• Fluorinert FC-75 • Nitric acid of 30 % or more of

concentration
• Perfluoro benzene
• Sulfuric acid of 40 % or more of
• Perfluoro octane concentration
• Perfluoro decalin • Hydrogen peroxide
Prominence-i LC-2030 LC-2030C LC-2030C 3D xv

Introduction
If PEEK resin parts are used for tubing connections, do not use the following
mobile phases.
These mobile phases weaken PEEK resin, which may lead to cracked tubing and
mobile phase leaks.
• Concentrated sulfuric acid • Dichloromethane

• Concentrated nitric acid • Chloroform
• Dichloroacetic acid • Dimethyl sulfoxide (DMSO)
• Acetone*1 • Fluorine organic solvents such as
• Tetrahydrofuran (THF) hexafluoroisopropanol (HFIP)
*1 There is no problem with temporarily using low-concentration aqueous solutions
with an acetone concentration of 0.5 % or less, e.g. for the purpose of checking
the performance of the gradient.
• Use only HPLC grade or comparable mobile phase, and filter it with a filter
of 0.45 µm mesh or finer before use to remove particulates and foreign matter.
• Halogen ions can corrode the stainless steel material (SUS316L) used in the
plumbing, so if such materials are used for the wetted parts of the equipment,
avoid, as much as possible mobile phases that contain halogen ions - such as
KCl, NaCl and NH4Cl or mobile phases that generate halogen ions in certain
reactions. If such mobile phases must be used, clean all flow lines thoroughly
with distilled water immediately after analysis.
• Avoid ammonia aqueous solution at a concentration exceeding 0.1 %. It may
damage the plunger of the pump and shorten the service life of the plunger
and the plunger seal.
• When an absorbance detector or photodiode array detector is used for high-
sensitivity analysis, be sure to use HPLC grade mobile phases that have a low
absorptivity of UV rays.
• Always degas the mobile phase, as air bubbles may tend to form during solvent
mixing or during temperature or pressure changes. Air bubbles may cause
pump malfunctions and detector signal noise.
• Understand the properties, including boiling points, firing point and viscosities,
of the mobile phase.
xvi Prominence-i LC-2030 LC-2030C LC-2030C 3D

Warning Labels
Warning Labels
In order to ensure safety, warning labels are attached in places requiring caution.
If a warning label is lost or damaged, obtain a new label through your Shimadzu
representative and attach it in the correct position.
Warning
No. Description
Label
(Part No. S037-72999-02)
1 During operations such as calibration, the autosampler operates even
with the front panel open. Do NOT touch the needle moving parts.
(Part No. S228-57699)

2 Do NOT forget to tighten tubing completely. Otherwise, leakage
may occur.
(Part No. S206-77586)

3 Be careful not to touch when the operating temperature of the
column oven is high (60 °C or over).
(Part No. S037-70854-02)

4 The lamp emits ultraviolet light when illuminated. Do NOT look
directly at the lamp light.
(Part No. S037-72999-12)
Allow the lamp and lamp mounting parts to cool down adequately
5
before replacing the lamp. The lamp compartment is extremely hot
just after turning off the lamp.
Prominence-i LC-2030 LC-2030C LC-2030C 3D xvii

Introduction
Warning
No. Description
Label
(Part No. S228-53303)
If a drain tube is not connected, the rinse solution may leak,
6 resulting in malfunction of the instrument. When a model with a
sample cooler is used, the condensation water may leak, resulting
in malfunction of the instrument.
(Part No. S228-53303)
7 If a drain tube is not connected, leaked solutions will not drain,
resulting in malfunction of the instrument.
(Part No. S228-53303)
If the column oven temperature is set to below the room
8 temperature, condensation may occur. If a drain tube is not
connected, leakage may occur, resulting in malfunction of the
instrument.
(Part No. S228-53303)
9 Be careful of dirt on the air filter. If the filter is clogged with dust,
the instrument's performance may be impaired.
xviii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Warranty
Warranty
Shimadzu provides the following warranty for this product.
1. Period:
Please contact your Shimadzu representative for information about the period of this warranty.
2. Description:
If a product/part failure occurs for reasons attributable to Shimadzu during the warranty period,
Shimadzu will repair or replace the product/part free of charge. However, in the case of products
which are usually available on the market only for a short time, such as personal computers and
their peripherals/parts, Shimadzu may not be able to provide identical replacement products.
3. Limitation of Liability:
(1) In no event will Shimadzu be liable for any lost revenue, profit or data, or for special, indirect,
consequential, incidental or punitive damages, however caused regardless of the theory of
liability, arising out of or related to the use of or inability to use the product, even if Shimadzu
has been advised of the possibility of such damage.
(2) In no event will Shimadzu's liability to you, whether in contract, tort (including negligence), or
otherwise, exceed the amount you paid for the product.
4. Exceptions:
Failures caused by the following are excluded from the warranty, even if they occur during the
warranty period.
(1) Improper product handling
(2) Repairs or modifications performed by parties other than Shimadzu or Shimadzu designated
companies
(3) Product use in combination with hardware or software other than that designated by Shimadzu
(4) Computer viruses leading to device failures and damage to data and software, including the
product's basic software
(5) Power failures, including power outages and sudden voltage drops, leading to device failures
and damage to data and software, including the product's basic software
(6) Turning OFF the product without following the proper shutdown procedure leading to device
failures and damage to data and software, including the product's basic software
(7) Reasons unrelated to the product itself
(8) Product use in harsh environments, such as those subject to high temperatures or humidity levels,
corrosive gases, or strong vibrations
(9) Fires, earthquakes, or any other act of nature, contamination by radioactive or hazardous
substances, or any other force majeure event, including wars, riots, and crimes
(10) Product movement or transportation after installation
(11) Consumable items

Recording media such as floppy disks and CD-ROMs are considered consumable items.
* If there is a document such as a warranty provided with the product, or there is a separate contract
agreed upon that includes warranty conditions, the provisions of those documents shall apply.
Prominence-i LC-2030 LC-2030C LC-2030C 3D xix

Introduction
After-Sales Service and Availability of Replacement Parts

n After-Sales Service
If any problem occurs with this product, perform an inspection and take appropriate
corrective action as described in "6 Troubleshooting" of the System Guide.
If the problem persists, or the symptoms are not covered in the troubleshooting section,
contact your Shimadzu representative.
n Replacement Parts Availability
Replacement parts for this product will be available for a period of seven (7) years after
the product is discontinued. Thereafter, such parts may cease to be available.
Note, however, that the availability of parts not manufactured by Shimadzu shall be
determined by the relevant manufacturers.
n Hardware Validation
Each unit in the instrument and the entire LC system should be checked periodically to
ensure that they function normally, else the analysis data may not be reliable.
Therefore, it is necessary to carry out periodic hardware validation and keep records of
the validation.
Before shipment from the factory, this instrument was rigorously inspected. The results
are summarized in the Inspection Certificate accompanying the instrument. To inspect the
instrument performance after installation, repeat the Hardware Validation as described
in "2 Validation" of the Integrity Guide.
n Hardware Validation Contract
This is a contract under which a qualified Shimadzu-approved engineer performs periodic

validation, and provides reports of the results.
Details of the contract can be obtained from your Shimadzu representative.
Maintenance, Inspections, and Adjustment

In order to maintain the instrument's performance and obtain accurate measurement
data, daily inspection and periodic inspection/calibration are necessary.
• For daily maintenance, inspection, and replacement parts, refer to the System Guide
and Maintenance Guide.
• Periodic inspection/calibration should be requested to your Shimadzu representative.
• Replacement cycles described for periodic replacement parts are rough estimate.
Replacement may be required earlier than the described replacement cycles depending
on usage environment and frequency.
xx Prominence-i LC-2030 LC-2030C LC-2030C 3D

Disposal Precautions
Disposal Precautions
When disposing of the instrument, contact your Shimadzu representative.
If you dispose them yourself, do so in accordance with the processing standards
determined by law, separately from general industrial waste and household garbage.
Precautions on Handling Deuterium (D2) Lamp
n When Disposing of the Lamp
If the deuterium (D2) lamp should be broken or its life is finished, dispose of the lamp
separately from general garbage. When disposing of the deuterium (D2) lamp provided
from Shimadzu Corporation, select a method, which will not harm the environment or
cause bodily injury. Consult your local Government Agencies for a proper disposal method.
The materials of deuterium (D2) lamp are as follows:

• Metals (Tungsten, Aluminum)
• Quartz glass
• Ceramic
• Plastic
Prominence-i LC-2030 LC-2030C LC-2030C 3D xxi

Contents
Introduction Indications Used in This Manual ......................... iii
Safety Instructions ............................................. iv
Static Electricity Precautions ............................... xi
Precautions for Mobile Phase Selection and Use xv
Warning Labels .............................................. xvii
Warranty ........................................................ xix
After-Sales Service and Availability of
Replacement Parts ............................................ xx
Maintenance, Inspections, and Adjustment ......... xx
Disposal Precautions ........................................ xxi
1 Maintenance 1.1 Periodic Inspection/Maintenance ........................... 1

1.1.1 Prior to Inspection/Maintenance ................................ 1
1.1.2 Periodic Inspection/Adjustment Work List ...................... 2
1.1.3 Daily Inspection ................................................. 4
1.1.4 Maintenance During Downtime (1 Week Min.) ................ 5
1.1.5 Maintenance During Continuous Use (1 Week Min.) ......... 6
2 Common Operation 2.1 Displaying the Replaced Part Items ....................... 7

2.2 Recording Replacement History in the
Maintenance Log ............................................... 10
3 Autosampler 3.1 Removing/Attaching the Front Panel ................... 12

3.2 Replacing the Needle Seal ................................. 14
3.3 Replacing the Needle ......................................... 18
3.4 Inspecting (Replacing) the High-Pressure Valve
Rotor and High-Pressure Valve Stator ................. 24
3.5 Replacing the Sample Loop ................................ 32
3.6 Replacing the Measuring Pump Plunger Seal ....... 39
3.7 Inspecting (Replacing) the Measuring Pump
Plunger ............................................................. 44
3.8 Inspecting (Replacing) the Low-Pressure Valve
Rotor and Low-Pressure Valve Stator .................. 49
xxii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Contents
3.9 Replacing the Rinsing Port Cap .......................... 55

3.10 Replacing the Rack Panel ................................... 57
3.11 Replacing the Front Panel .................................. 58
3.12 Replacing the Wiper .......................................... 59
3.13 Cleaning/Inspecting (Replacing) the Suction
Filter ................................................................ 60
3.14 Rinsing the Flow Line ........................................ 62
3.14.1 Rinsing the Needle and Sample Loop ........................ 62
3.14.2 Performing Reverse Rinsing of the Flow Line ................ 62
3.15 Replacing the Outlet Tubing .............................. 64
4 Pump 4.1 Attaching/Removing the Pump Heads .................. 66

4.2 Replacing the Plunger Seal ................................ 70
4.3 Cleaning/Inspecting (Replacing) the Plunger
and Diaphragm ................................................. 76
4.4 Rinsing the Check Valve .................................... 86
4.5 Performing an Ultrasonic Cleaning of the
Check Valve ...................................................... 89
4.6 Inspecting (Replacing) the Check Valve ............... 91
4.7 Inspecting the Line Filter ................................... 95
4.8 Replacing the Line Filter .................................... 97
4.9 Cleaning/Inspecting (Replacing) the Suction
Filter ................................................................ 99
4.10 Replacing the Drain Valve ASSY ....................... 101
4.11 Cleaning of the Automatic Rinsing Kit .............. 103
5 UV Detector 5.1 Removing/Attaching the Flow Cell .................... 104

5.2 Flow Cell Inspection / Simple Cleaning .............. 107
5.3 Disassembling and Cleaning the Flow Cell ......... 110
5.4 Replacing a Flow Cell Part ............................... 115
5.5 Replacing the D2 Lamp .................................... 120
6 PDA Detector 6.1 Removing/Attaching the Flow Cell .................... 124

6.2 Flow Cell Inspection / Simple Cleaning .............. 127
Prominence-i LC-2030 LC-2030C LC-2030C 3D xxiii

Contents
6.3 Disassembling and Cleaning the Flow Cell ......... 130

6.4 Replacing a Flow Cell Part ............................... 135
6.5 Replacing the D2 Lamp .................................... 140
6.6 Replacing the W Lamp (Optional Part) .............. 144
Other 149
7 Inspection/Maintenance
7.1 Cleaning the Leak Tray ....................................
7.2 Replacement of the Air Filter ........................... 150

7.2.1 Side Air Filter ................................................ 150
7.2.2 Air Filter for UV/PDA Detector .............................. 152
7.2.3 Air Filter for the Autosampler .............................. 154
8 Auxiliary Functions 8.1 Displaying Auxiliary Function Items ................... 155

8.2 Z-Home ........................................................... 157
8.3 Pump Pressure Zero Adjustment ....................... 157
8.4 High-Pressure Valve Operation Check ................ 158
8.5 Autosampler LED Operation Check .................... 159
8.6 Autosampler Sensor Test .................................. 160
8.7 Injection Port Operation Check ......................... 161
Index ......................................................... 162
xxiv Prominence-i LC-2030 LC-2030C LC-2030C 3D

1 Maintenance
1.1 Periodic Inspection/Maintenance 1
Periodic inspections of instrument must be performed to ensure safety during use.
It is possible to have these periodic inspections performed by a Shimadzu field engineer
on a contractual basis.
Contact your Shimadzu representative for the details of the maintenance/inspection
contract.
Be sure to power OFF the instrument and pull out the plug from the power
supply before inspection/maintenance unless otherwise instructed.
Failure to observe the above instructions may cause a fire, electrical shock, and
device failure.
Never remove the cover.

This may cause injury or a malfunction of the device. Contact your Shimadzu
representative if the main cover must be removed.
For parts replacement, use parts and tools listed in "1.2 Configuration parts"
and "7.2 Consumable Parts / Maintenance Parts / Replacement Parts / Tools"
of "SYSTEM GUIDE".
If any other parts or tools are used, part damage, injury, and malfunction may
occur.
1.1.1 Prior to Inspection/Maintenance
• Purge the mobile phase solution in the flow lines with water.
• Wipe away any dirt from the main cover.
• Wipe away any dirt from the monitor screen with tissue paper or a soft cloth moistened
with water.
• Be sure to stop pumping before maintenance.
• When starting pumping after maintenance, be sure to reattach the front panel.
• Be sure to follow the instruction manuals and screen instructions during
inspection/maintenance.
Prominence-i LC-2030 LC-2030C LC-2030C 3D 1

1 Maintenance
1.1.2 Periodic Inspection/Adjustment Work List
The [Replacement Parts Selection] screen displays items for which maintenance is
necessary. The displayed replacement/inspection periods are set only as criterion values.
They do not indicate the warranty period. The periods vary depending on the conditions
of use.
n Items requiring maintenance based on usage frequency as criterion value
Autosampler
Inspection and Maintenance

Maintenance Guidelines Ref.
Items
Replacement of Needle Seal Replace about every 40,000 injections. P.14
Replacement of Needle Replace about every 40,000 injections. P.18
Replace about every 100,000 rotations.
Inspection (Replacement) of
(Only when using mixtures of water and organic
High-Pressure Valve Rotor
solvent)
P.24
Replace about every 100,000 rotations.
(Only when using mixtures of water and organic
High-Pressure Valve Stator
solvent)
Replacement of Sample Loop Replace about every 40,000 injections. P.32
About every 60,000 rotations
Low-Pressure Valve Rotor
P.49
About every 120,000 rotations
Low-Pressure Valve Stator
Replacement of Rinsing Port
Replace about every 10,000 injections. P.55
Cap
n Items requiring maintenance based on period of use as criterion value
Autosampler
Inspection and 1 2 3
Remarks Ref.
Maintenance Items Year Years Years
Replacement of
Measuring Pump - P - - P.39
Plunger Seal
Inspection
(Replacement) of
- - P - P.44
Measuring Pump
Plunger
Inspection Using the period on the left as a
(Replacement) of - P - guideline, replace when solid matter P.60
Suction Filter in the rinse solution causes clogging.
Replace when the clogging of the
tubing is irremovable despite having
Replacement of SUS
P*1 - - followed the guideline*1 on the left P.64
Pipe HP OUT
and having carried out a reverse
rinse.
2 Prominence-i LC-2030 LC-2030C LC-2030C 3D

1.1 Periodic Inspection/Maintenance
Remarks Ref.
Using the period on the left as a
Replacement of Rack guideline, replace when the
Panel
- - P
condensation at the sample cooler
P.57 1
portion worsens.
Replacement of Front
- - P guideline, replace when the sample P.58
Panel
rack insertion port becomes worn.
Replacement of Wiper - - P guideline, replace when the wiper P.59
becomes worn.
Cleaning/Lubrication
Contact your Shimadzu
of Needle Drive P - - -
representative.
Section (Z Mount)
Cleaning/Lubrication
Contact your Shimadzu
of Other Drive - - P -
representative.
Sections
*1 This is a guideline when using the optional extra tubing with an inside diameter of 0.1. Replacement
is not necessary when using standard accessory tubing with an inside diameter of 0.3 or when no
abnormality is found.
Pump
Remarks Ref.
When replacing the plunger, replace
the plunger seal as well.
Use the pumping amounts below as
a guideline for replacing the
plunger seal when delivering 2-
propanol.
Replacement of
P - - Pumping P.70
Plunger Seal Pumping Pressure
Amount
10 MPa (102
90 L
kgf/cm2)
30 MPa (306
30 L
kgf/cm2)
Inspection
(Replacement) of P - - - P.76
Plunger
Inspection
When replacing the plunger, replace
(Replacement) of P - - P.76
the diaphragm as well.
Diaphragm
Inspection
(Replacement) of P - - - P.91
Outlet Check Valve
Inspection
(Replacement) of Inlet P - - - P.91
Check Valve

1 Maintenance
Remarks Ref.
(Replacement) of Line - P - guideline, replace when solid matter P.97
Filter in the mobile phase causes clogging.
(Replacement) of - P - guideline, replace when solid matter P.99
Suction Filter in the mobile phase causes clogging.
(Replacement) of the - - P guideline, replace when there is a P.101
Drain Valve fluid leak from the drain valve.
Lubrication of the Contact your Shimadzu
- - P -
Pump ASSY representative.
Cleaning of the
P - - - P.103
Automatic Rinsing Kit
Detector
Remarks Ref.
Inspection/Simple Using the period on the left as a
Cleaning of the Flow P - - guideline, clean the flow cell when P.107
Cell air bubbles and dirt are found.
Disassembling and guideline, clean the flow cell when
Cleaning of the Flow - P - the dirt inside is hard to remove. P.110
Cell Be sure to replace the flow cell
gasket.
Replacement of Lens
and Cell Window for - - P - P.115
Flow Cell
As a guideline, replace about every
Replacement of D2
- - P 2,000 hours of total illumination P.120
Lamp
time.
As a guideline, replace about every
Replacement of W
- - P 2,000 hours of total illumination P.144
Lamp (Optional Part)
time.
1.1.3 Daily Inspection
n Before use
Do not operate the device repeatedly when the flow line of the pump and autosampler
are not filled with mobile phase. Doing so causes quick consumption of the plunger seal
and plunger of the pump and the high-pressure valve rotor and high-pressure valve stator
of the autosampler.

1.1 Periodic Inspection/Maintenance
n After use
• Rinse the flow line with distilled water or organic solvent before extended periods of
downtime.
1
• If buffer solution was used as mobile phase solution or rinse solution, be sure to rinse
the solution with distilled water or de-ionized water after operation. Otherwise, crystals
formed from residual buffer solution due to volatilization may cause device failure and
clogging in the flow line of the pump and autosampler.
1.1.4 Maintenance During Downtime (1 Week Min.)
• After rinsing the flow line, put the needle in the up status to prevent deterioration of
the needle seal life during extended periods of instrument downtime.
• "3.14 Rinsing the Flow Line" P.62
• "8.2 Z-Home" P.157
• Ventilate the autosampler inside by pulling out the sample rack for about 1 day.

1 Maintenance
1.1.5 Maintenance During Continuous Use (1 Week Min.)
Condensation water may collect on the cooling surface of the sample rack between the
sample rack and the rack plate as well as in the column oven if the device is used
continuously for an extended period of time with the set temperature of the sample
cooler and column oven lower than room temperature.
Periodically wipe away the condensation water with wiping paper.
n Sample rack
1 Turn OFF (Power button).
2 Remove the front panel.

"3.1 Removing/Attaching the Front Panel" P.12
3 Wipe off the condensation water from the rack plate with wiping paper.
Do NOT touch the tip of the needle.

If the needle is touched by hand, the tip of the needle may cause a hand injury.
4 Attach the front panel.

n Column oven leak tray
1 Open the column oven door.
2 Wipe off the condensation water from the lower column oven tray with wiping
paper.
3 Close the column oven door.

2 Common Operation
2.1 Displaying the Replaced Part Items
1 Press (Menu).
The [Menu] screen opens. 2
2 Press [Maintenance].
The [Maintenance] screen is displayed. The
tab for the replaced part is displayed first.
• Press (Replacement
part) to display the tab for

the replaced part when other
tabs are displayed in the
[Maintenance] screen.
• When a service duration for a
part in the unit exceeds the
replacement guidelines
settings, is displayed.

2 Common Operation
3 Press the button to select each unit.

The [Replacement Parts Selection] screen is
displayed. The tab for the selected unit is
displayed.
Press the button with the corresponding

name to display the replacement screen for
a main part. Go to Procedure 5.
If a W lamp is connected, press

[D2 Lamp / W Lamp] once to
alternatively switch the [D2
Lamp] and [W Lamp] displays.
Pressing the button displayed as
"Usage frequency / Replacement
guidelines" displays the
replacement screen for the
corresponding part.
4 Press an item of the part for

replacement.

2.1 Displaying the Replaced Part Items
5 Carry out the operation by following the explanation in the screen.

Operations vary depending on each item. The following is an explanation using [Plunger]
as an example.
No. Name Explanation

1 Operations Guide Area Displays the operating instructions and buttons.
2 Entire Procedure Displays the entire procedure.
Stop Cancels the operation and closes the screen.
Back Displays the previous screen.
Next Displays the next screen.
Displayed when the results are given after all the
Finish procedures are finished.
Closes the screen.
3 Current Procedure Displays the current procedure position in light gray.

2 Common Operation
2.2 Recording Replacement History in the Maintenance Log

When replacing a part not displayed in the [Replacement Parts Selection] screen, record
the replacement history in the maintenance log by inputting the part No. Input operation
is not necessary for parts displayed in the [Replacement Parts Selection] screen because
the replacement history is recorded automatically in the maintenance log when the on-
screen instructions are followed.
1 Press (Menu).
The [Menu] screen opens.
The [Maintenance] screen is displayed. The
tab for the replaced part is displayed first.
• Press (Replacement
part) to display the tab for

the replaced part when other
tabs are displayed in the
[Maintenance] screen.
• When replacement of a part
inside the unit is necessary,
is displayed.
3 Press [Others].
The [Replacement Parts Selection] screen is
displayed. The [Others] tab is displayed.

2.2 Recording Replacement History in the Maintenance Log
4 Press [Others].
The [Other Part Replacement] screen is
displayed.
5 Input [P/N] (Part No.), then press [OK].

The confirmation screen is displayed.
6 Check the part No., then press [OK].

The input part No. is recorded in the
maintenance log.

3 Autosampler
3.1 Removing/Attaching the Front Panel
Before performing maintenance, etc., of the needle, needle seal, or high-pressure valve,
the front panel must first be removed.
n Removing the front panel
1 Remove the inserted sample racks (A)

and open the panels and doors (B) as
shown in the diagram.
2 Remove the 4 knurled screws (A), and

pull the front panel (B) forward.
The 2 screws on the top side are short and
the 2 screws on the right side are long.

3.1 Removing/Attaching the Front Panel
n Attaching the front panel
1 Align the groove (b) on the bottom of

the front panel with the protrusion on
the main body frame (a) to insert the
front panel.
2 Insert the bundle of tubes (C) into the

opening (c) on the left side of the
front panel.
3 Attach the 4 knurled screws (A).

3
If the front panel (A) is not
attached correctly, condensation
may settle on the rack plate.
4 Attach the removed sample racks (A)

and close the panels and doors (B) as
shown in the diagram.

3 Autosampler
3.2 Replacing the Needle Seal
Necessary tools
Wrench (10 mm)
Needle seal XR tool (Part No.: S228-50570)
Necessary parts
Part Name Part Type Part No. Remarks

Needle Seal XR Consumable part S228-50390 -
Housing, Replacement
S228-50391 -
Needle Seal XR part
Needle Seal XR Replacement The set includes 1 housing and
Kit (Containing 3 part/ S228-50452-93 3 pieces of Needle Seal XR
needle seals) Consumable part (S228-50390).
To use solvents for the GPC
method, such as
Vespel® needle seal
Optional part S228-50390-41 tetrahydrofuran (THF) and
XR
chloroform. Applicable pH
range is "1-9".
1 Press [Needle Seal] on the [Replacement Parts Selection] screen.

"2 Common Operation" P.7
The [Replacing the needle seal] screen is displayed.
The [Replacing the needle seal] screen can also be displayed by pressing [Needle
Seal] on the replacement parts tab in the [Maintenance] screen.
2 Press [Power OFF].

The Confirmation screen opens. Press [OK].
The needle moves to the center of the
instrument and the power is turned OFF.

3 Replace the needle seal.

2 Use a 10 mm wrench (B) to

remove the cap (A).
3 The needle seal is now exposed.

Use the needle seal tool (B) to
loosen and remove the housing
(A).

3 Autosampler
4 Push the bottom of the needle

seal (a) to remove the needle seal
(A) from the housing (B).
If it is difficult to remove the

needle seal, do NOT use
excessive force to remove it.
Applying excessive energy may
result in injury. If the needle
seal cannot be removed,
replace the needle seal and the
housing at the same time.
5 Attach the housing (A) to the

high-pressure valve, and use the
needle seal tool (B) to tighten it.
6 Insert a new needle seal (A) into

the housing (B).
When inserting the needle

seal, use clean wiping paper to
hold the needle seal while
taking care to keep the hole
dust- and dirt-free.
Dust or dirt creates a
microscopic gap that may cause
carryover or liquid leakage.

7 Firmly finger-tighten the cap (A)

to the housing, and then use a
10 mm wrench (B) to tighten and
turn the cap 90°.

4 Press (Power button) to turn ON.
After the instrument starts, input the login pass ID Number and press [OK] to display
the [Replacing the needle seal] screen.
5 Press the [Next] button.

The next screen is displayed and the usage
value is reset. The replacement history is
recorded in the maintenance log.
6 Check that the usage value is reset and

then press the [Next] button.
The next screen is displayed.

3 Autosampler
7 Press [Start].
Conditioning is performed.
Conditioning creates a smooth seal

surface between the needle and the
needle seal.
After conditioning is finished, the message

"Press "Exit" to complete parts
replacement." is displayed.
8 Press [Exit].
3.3 Replacing the Needle
Necessary tools
Wrench (6 mm)
Necessary parts

The needle base is covered with a green
Consumable
Needle S228-41024-99 tube for an identification mark.
part
A ferrule and male nut are provided.
1 Press [Needle] on the [Replacement Parts Selection] screen.

The [Replacing the needle] screen is displayed.
The [Replacing the needle] screen can also be displayed by pressing [Needle] on
the replacement parts tab in the [Maintenance] screen.

2 Press [Close].
3 Replace the needle.

3
2 Open the clamp (B) on the left

side of the Z mount cover (A),
and remove the flat cable (C)
from the Z mount cover.
If the clamp (B) is

stiff and difficult to
open, hold the Z
mount cover (A) with
one hand and open
the clamp (B).
3 Hold both sides of the Z mount

cover bottom (a), and pull
forward to remove the cover.

3 Autosampler
4 Use a 6 mm wrench to loosen the

male nut (A) and remove the
needle (B).
5 Attach the male nut (B) and the

ferrule (C) to the new needle (A).
Firmly finger-tighten the nut, and
use a wrench to tighten it 180°.
Insert the needle until it touches the back of the connection hole, and then
tighten using a wrench.
If the needle is not fully inserted and does not reach the back of the
connection hole, a dead volume will be created and may cause a broad peak
or cross contamination.
Tighten the male nut fixing the needle 180°.

Also, be sure to use the provided Ferrule 1.2F.
Using a regular ferrule (Ferrule 1.6F) may cause liquid leakage.

6 Align the protrusion (a) on the

top of the Z mount with the slit
(b) on the top of the Z mount
cover and fit them together. Hold
the bottom of the Z mount from
both sides, and fit the right and
left catches (c) on the bottom of
the Z mount cover to the Z
mount bottom.
7 Pass the flat cable (A) through 3

the clamp (B) and close the clamp.
If the clamp (B) is

close, hold the Z
mount cover with
one hand and close
the clamp (B).
After inspection and replacement, be sure to attach the Z mount cover and
clamp the flat cable.
Failure to do so may result in the cable interfering with another internal part
and causing abnormal operation or a malfunction.
After Z mount cover is installed, check that Z mount cover does not come off
with lightly pulling it forward.
If Z mount cover is not correctly installed, it may drop, resulting in damaging
the parts of the autosampler.

3 Autosampler
the [Replacing the needle] screen.
With the front panel not attached, press (Power button) to turn ON.
Never put hands inside the instrument during autosampler operation.

Failure to comply with the above precaution may result in injury from the
needle. During checking and adjustment of the needle position, operation
continues even when the front panel is open.

value is reset.
The replacement history is recorded in the
maintenance log.


7 Press [Start] to check that the needle

(A) and the injection port (B) are
correctly aligned.
At the position where the tip of the

needle seals the needle seal, perform an
up-and-down movement of about 2 mm 2
times. At this time, check that the needle
has moved directly down to the injection
port without moving left, right, forward,
3
or backward.
If the needle position is out of

alignment, press [Stop] to cancel
the task, and perform [Inj. Port
Adjustment] for calibration.
Integrity Guide "3.3.3 Adjusting the

Injection Port Position"
8 Press [Next].


3 Autosampler
10 Press [Start].
Conditioning creates a smooth seal

surface between the needle and the
needle seal.

11 Press [Exit].
When the same needle is

reattached during maintenance
or other tasks, if cross
contamination increases, replace
with a new needle.
3.4 Inspecting (Replacing) the High-Pressure Valve Rotor and High-Pressure Valve Stator
Necessary tools
Wrench (6 mm)
Allen wrench (M4)
Necessary parts

High-Pressure Valve Consumable
S228-56098 -
Rotor part
High-Pressure Valve Consumable
S228-56253-42 -
Stator part
When using solvents such as
High-Pressure Valve Replacement tetrahydrofuran (THF), as a general
S228-56061
Gasket part guideline replace the gasket after
about 100,000 injections.
Replace the housing when the
High-Pressure Valve Replacement tubing connection has worn out
S228-56278-41
Housing part due to repeated attaching and
removing of the tubing.

1 Fill the mobile phase A flow line with an organic solvent such as 2-propanol,
methanol, or acetonitrile.
After the high-pressure valve rotor and stator have been replaced, perform
conditioning by pumping of the mobile phase A in order to create a smooth
seal surface (switching back and forth 50 times).
2 Press [HPV Rotor] or [HPV Stator] on the [Replacement Parts Selection] screen.
The [High pressure valve rotor and stator replacement] screen is displayed.
The [High pressure valve rotor and stator replacement] screen can also be 3
displayed by pressing [HPV Rotor] or [HPV Stator] on the replacement parts tab
in the [Maintenance] screen.


3 Autosampler
4 Replace the high-pressure valve rotor and the high-pressure valve stator.

2 Use a 6 mm wrench to loosen all

male nuts (A) of the flow line
tubing and remove the tubing
from the high-pressure valve.
There is no need to
remove the needle
seal.
3 Use an M4 Allen wrench to

loosen the 3 hexagon socket
screws in the order of A, B, and
C by turning each screw 60°.
If only certain screws

are loosened, the
stator may become
tilted, making it
difficult or
impossible to remove
the stator or
resulting in a seized
screw.
4 Hold the high-pressure valve

stator ASSY (A) and the ring (B),
and remove the ring taking care
not to drop it.
Be sure to remove the ring

and the stator ASSY together.
If trying to remove only the
stator ASSY, the ring may
drop, resulting in damage or
injury.

5 Hold the outside (a) of the high-

pressure valve rotor (A) and
remove the rotor.
6 Check that the rotating and

sliding surfaces (a, b) of the high-
pressure valve stator (A) and the
high-pressure valve rotor (B) have 3
no scratches.
• If a scratch is
found through
visual inspection,
replace with a new
high-pressure valve
rotor and high-
pressure valve
stator.
• Any scratches can
cause wear and
tear in a short
period of time.
7 Remove the cap (A) to

disassemble the high-pressure
valve stator ASSY into the high-
pressure valve stator (B), gasket
(C), backup ring (D), and housing
(E).

3 Autosampler
8 Place the high-pressure valve

rotor (A), high-pressure valve
stator (B), gasket (C), and housing
(D) into a beaker filled with 2-
propanol, and clean for 5 minutes
in an ultrasonic bath.
9 Align the notch (a) on the high-

pressure valve rotor (A) with the
alignment mark (b) of the shaft
(B), and gently push and fit them
together.
As much as possible,
touch only the very
edge of the high-
pressure valve rotor.
After fitting, use
wiping paper
moistened with 2-
propanol to wipe the
rotor sliding surface
that contacts the
sliding surface of the
high-pressure valve
stator.
10 Attach the ring (B) to the high-

pressure valve main unit (A).

11 Reassemble the components onto

the housing (A) in the following
order: the backup ring (B), gasket
(C), and high-pressure valve stator
(D). Fit the cap (E) to complete
reassembly of the high-pressure
valve stator ASSY.
Be sure to attach the backup

ring.
The backup ring is fitted to
prevent the stator and the
gasket from becoming tilted. If
not attached, it may cause 3
liquid leakage or wear and
tear on the rotor and stator in
a short period of time.
12 Use wiping paper moistened with

2-propanol to wipe the sliding
surface (a) of the high-pressure
valve stator.
13 Fit the high-pressure valve stator

ASSY (A) onto the high-pressure
valve main unit. Use a finger to
lightly press the center down
while ensuring the high-pressure
valve stator ASSY (A) is level.
14 While pressing on the high-

pressure valve stator ASSY (A),
insert the longer side of the Allen
wrench (M4) (B) into each of the
3 hexagon socket screws (C) and
lightly turn to tighten until the
screw head makes contact.

3 Autosampler
15 Use an Allen wrench (M4) to

tighten each of the 3 hexagon
socket screws 60° in the order of
A, B, and C.
16 Loosen each of the 3 hexagon

socket screws 60° from fully
tightened in the order of C, B,
and A 3 times. (Loosen each screw
a total of 180°.)
17 Once again, repeatedly tighten
each screw 60° in the order of A, B, and C until completely tightened.
Use the longer side of the Allen wrench as a handle to further tighten.
If the screws are inadequately tightened, the high-pressure valve stator ASSY
may be installed at an angle and result in liquid leakage or cause wear and
tear of the rotor and stator in a short period of time.
18 Reattach the flow line tubing of

each high-pressure valve port to
their original positions, and use a
6 mm wrench to tighten the 5
male nuts (A).
19 Lightly move the Z mount (A) by

hand, and check that the bottom
of the Z mount does not interfere
with the tubing (B) connected to
the high-pressure valve.
Move the Z mount

by holding the
metallic section.


the [High pressure valve rotor and stator replacement] screen.
6 Select the replaced part and press

[Next].
value is reset.
maintenance log.

then press [Next].
8 Press [Start].
• Because abrasion powder may be generated initially, be sure to remove the

column before performing conditioning. To create a smooth seal surface
between the high-pressure valve rotor and stator, perform conditioning by
pumping of the mobile phase A at 1 mL/min (switching back and forth 50
times).
• Check for liquid leakage from gaps around the high-pressure valve.

3 Autosampler
9 Press [Exit].
3.5 Replacing the Sample Loop
Do NOT frequently bend and straighten in the same place.

If the tubing is repeatedly bent in the same place, the tubing will weaken,
which may cause a rupture or cracks resulting in leakage of solvent. The
performance of the instrument or the sample loop life is also significantly
affected.
Do NOT crush or bend tubing at a sharp angle using pliers, etc.

When bending stainless steel tubing, if the circular arc of the bend (bend
radius) is small, the inside shape of the tubing will become deformed, which
may cause a blockage in the tubing or an increase in pressure. The performance
of the instrument or the sample loop life is also significantly affected.
Necessary tools
Wrench (6 mm)
Necessary parts

Sample Loop This is the sample loop for 100
Consumable part S228-56074-42
for 100 µL Injection µL injections.
Sample Loop This is the sample loop for
Optional part S228-56074-44
for 50 µL Injection 50 µL injections.
This is the extended sample
Extended Sample loop kit for 500 µL injections.
Loop Kit Optional part S228-45405-41 For installation, refer to the
for 500 µL Injection instruction manual provided
with the part.
This is the extended sample
Extended Sample loop kit for 2 mL injections.
Loop Kit Optional part S228-45405-42 For installation, refer to the
for 2 mL Injection instruction manual provided
with the part.

1 Press [Sample Loop] on the [Replacement Parts Selection] screen.

The [Replacing the Sample Loop] screen is displayed.
The [Replacing the Sample Loop] screen can also be displayed by pressing
[Sample Loop] on the replacement parts tab in the [Maintenance] screen.

3 Replace the sample loop.

2 Use a 6 mm wrench to remove

the male nut (A) that fixes the
sample loop to port 1 of the high-
pressure valve.

3 Autosampler
3 Open the clamp (B) on the left

side of the Z mount cover (A),
and remove the flat cable (C)
from the Z mount cover.
If the clamp (B) is

open, hold the Z
mount cover (A) with
one hand and open
the clamp (B).
4 Hold both sides of the Z mount

cover bottom (a), and pull
forward to remove the cover.

the male nut (A) that fixes the
sample loop to the needle.

6 Remove the sample loop from the

2 clamps (a) attached to the inner
front of the plastic cover.

3 hooks (b) attached to the inner
left side of the plastic cover.

rectangular socket holder (c)
located at the rear of the Z
mount.
9 To remove the sample loop from

the instrument, pass the section
of the sample loop that was 3
connected to the high-pressure
valve from the front to the back
through the gap (a) between the
upper left of the XY arm and the
plastic cover, then from the back
to the front through the gap (b)
around the rectangular socket
holder at the rear of the Z mount.
10 In the reverse order of removing

the old sample loop, pass the new
sample loop from the front to the
back through the gap (b) around
the rectangular socket holder at
the rear of the Z mount, and then
from the back to the front
through the gap (a) between the
upper left of the XY arm and the
plastic cover.
11 Attach the sample loop (A) to the

rectangular socket holder (B) at
the rear of the Z mount.

3 Autosampler
12 Fit the male nut and the ferrule

to the sample loop, then use a
6 mm wrench to tighten the male
nut (A) and fit the sample loop to
the joint section on the needle
side.
Insert the sample loop until it touches the back of the connection hole, and
then use a wrench to tighten it.
If the sample loop is not fully inserted and does not reach the back of the
connection hole, a dead volume will be created and may cause a broad peak
or cross contamination.
13 Attach the section (a) of the

sample loop that is positioned by
bands to the innermost hook (b)
attached to the inner left side of
the plastic cover.
As shown in the
diagram, to ensure
the band heads face
toward the left side
of the instrument,
twist about
90° counterclockwise
when seen from the
front of the
instrument, and
attach the sample
loop to the hook.
14 Fix the sample loop to the

remaining 2 hooks (c).
15 Fix the sample loop to the 2

clamps (d) attached on the inside
of the plastic cover.

16 Align the protrusion (a) on the

top of the Z mount with the slit
(b) on the top of the Z mount
cover and fit them together. Hold
the bottom of the Z mount from
both sides, and fit the right and
left catches (c) on the bottom of
the Z mount cover to the Z
mount bottom.
17 Pass the flat cable (A) through 3

the clamp (B) and close the clamp.
If the clamp (B) is

close, hold the Z
mount cover with
one hand and close
the clamp (B).
After inspection and replacement, be sure to attach the Z mount cover and
clamp the flat cable.
Failure to do so may result in the cable interfering with another internal part
and causing abnormal operation or a malfunction.
After Z mount cover is installed, check that Z mount cover does not come off
with lightly pulling it forward.
If Z mount cover is not correctly installed, it may drop, resulting in damaging
the parts of the autosampler.
18 Fit the male nut and the ferrule

to the sample loop, then use a
6 mm wrench to tighten the male
nut (A) and fit the sample loop to
port 1 of the high-pressure valve.

3 Autosampler
19 Slacken the sample loop fitted to

port 1 as shown in the diagram,
then adjust the tubing
configuration so that the lowest
point is positioned above the
high-pressure valve tray.
20 Move the Z mount by hand

toward the upper part of the
high-pressure valve, and check
that the sample loop does not
interfere with the Z mount
bottom.

the [Replacing the Sample Loop] screen.

value is reset.
maintenance log.


3.6 Replacing the Measuring Pump Plunger Seal
7 Enter the volume of the fitted sample

loop, and then press [Finish].
When replacing with a sample

loop for 50 µL or below, if the
sample aspiration rate is set to
a value greater than 5.0 µL/sec,
the sample aspiration rate will
be automatically changed to
5.0 µL/sec.
Operation Guide "5.2.2 Autosampler"
3.6 Replacing the Measuring Pump Plunger Seal 3

Necessary tools
Wrench (6 mm and 8 mm)
Allen wrench (M4)
Seal tool (Part No.: S228-25142-01)
Necessary parts
Part Name Part Type Part No.

Measuring Pump Plunger Seal Consumable part S228-35145
1 Press [Plunger Seal] on the [Replacement Parts Selection] screen.

The measuring pump plunger moves all the way back, and the [Measuring pump plunger
seal] screen is displayed.
2 Replace the measuring pump plunger seal.
1 Open the valve mount door.

3 Autosampler
2 Use a 6 mm and an 8 mm wrench

to loosen the 3 male nuts (A).
Remove the flow line tubing from
the top and bottom of the pump
head and from port 1 of the low-
pressure valve.

remove the 2 hexagon socket
screws (B) located on the pump
head (A).
4 Keep the pump head (A) level

and slowly pull it away from the
seal holder (B).
Keep the pump head level and

slowly pull it out.
The plunger may break if it is
pulled out at an angle.

5 Keep the seal holder (A) level and

slowly pull it away from the
plunger (B).
6 Vertically insert the end of the

seal tool (A) with a stepped side
(a) into the plunger seal (B).
3
7 Pull out the seal tool (A) vertically
to remove the plunger seal (B)
from the seal holder (C).
8 If the inside (a) of the pump

head or the fitting section (b) of
the plunger seal is dirty, use
wiping paper moistened with 2-
propanol to wipe clean.
Airtightness cannot
be maintained if
there is seal residue
or other matter
adhering to the
pump head or the
plunger seal.
9 Place a new plunger seal (B) on

the seal holder (C). Vertically
insert the non-stepped end (a) of
the seal tool (A) into the plunger
seal (B), and push down until it is
level.
10 Pull out the seal tool (A)

vertically.

3 Autosampler
11 Slowly push the seal holder (B)

with the alignment mark (a)
facing up onto the plunger (A).
Then, carefully push the pump
head (C) onto the seal holder
horizontally.
The plunger may

break if the pump
head is pushed in
with excessive force.
When pushing on the seal holder, the plunger seal may come off the seal
holder. To prevent the plunger seal from coming off, use wiping paper
moistened with 2-propanol or similar to hold the plunger seal while pushing
the seal holder.
12 Attach the 2 hexagon socket

screws (B) to the pump head (A),
and tighten with an M4 Allen
wrench.
Tighten the right

and left hexagon
socket screws
alternately 90° each,
using the longer side
of the M4 Allen
wrench as a handle
to firmly tighten
them at the end.
13 Reattach the tubing on the top

and bottom of the pump head
and the tubing to port 1 of the
low-pressure valve to the original
positions, and fix them using a
6 mm and 8 mm wrench.

14 Close the valve mount door.
3 Press [Next].
3
4 Press [Finish].
After finishing, perform a purge and check
for liquid leakage from any gap between
the pump head and the head holder or
the tubing connection parts.
maintenance log.
If liquid leakage is found even

after replacing the plunger seal,
the surface of the measuring
pump plunger may be
scratched. Inspect the plunger,
and if the measuring pump
plunger is scratched, replace
with a new plunger.
"3.7 Inspecting (Replacing) the Measuring Pump Plunger" P.44

3 Autosampler
3.7 Inspecting (Replacing) the Measuring Pump Plunger
Necessary tools
Allen wrench (M4)
Plunger tool (Part No.: S228-34672-02)
Necessary parts

Replacement
Measuring Pump Plunger S228-35010-91
part
1 Press [Plunger] on the [Replacement Parts Selection] screen.

The measuring pump plunger moves all the way back, and the [Measuring pump plunger]
screen is displayed.
2 Replace the measuring pump plunger.
2 Use a 6 mm and an 8 mm wrench

to loosen the 3 male nuts (A).
Remove the flow line tubing from
the top and bottom of the pump
head and from port 1 of the low-
pressure valve.


screws (B) located on the pump
head (A).
4 Keep the pump head (A) level

and slowly pull it away from the
seal holder (B).
Keep the pump head level and

slowly pull it out.
The plunger may break if it is
pulled out at an angle.
5 Keep the seal holder (A) level and

slowly pull it away from the
plunger (B).
6 Press [Plunger Operation].

The measuring pump plunger
moves toward the front of the
instrument.

3 Autosampler
7 Push the plunger tool (A) onto

the plunger (B), and turn the
plunger counterclockwise to
remove the plunger ASSY.
Be careful not to
break the plunger
when pushing the
plunger tool onto
the plunger.
8 Check that the plunger section (D)

is not scratched or dirty.
• If a scratch is found through

visual inspection, replace with
a new plunger.
• If the plunger is dirty, use
wiping paper moistened with
2-propanol to clean off the dirt on the inside of the pump head and on the
fitting section of the plunger seal.
Accuracy of the measurements may deteriorate if there is seal residue

or other matter adhering to the plunger.
9 Using wiping paper to hold the

plunger section (A), fit the
plunger to the measuring pump
main unit (B) and finger-tighten
lightly.
10 Push the plunger tool (A) onto

the plunger (B) and tighten the
plunger ASSY.

11 Press [Plunger Operation].

The measuring pump plunger
moves toward the back of the
instrument.
12 Slowly push the seal holder (B)

with the alignment mark (a)
facing up onto the plunger (A).
Then, carefully push the pump
head (C) onto the seal holder
3
horizontally.
The plunger may

break if the pump
head is pushed in
with excessive force.
When pushing on the seal holder, the plunger seal may come off the seal
holder. To prevent the plunger seal from coming off, use wiping paper
moistened with 2-propanol or similar to hold the plunger seal while pushing
the seal holder.

screws (B) to the pump head (A),
and tighten with an M4 Allen
wrench.
Tighten the right

and left hexagon
socket screws
alternately 90° each,
using the longer side
of the M4 Allen
wrench as a handle
to firmly tighten
them at the end.

3 Autosampler
14 Reattach the tubing on the top

and bottom of the pump head
and the tubing to port 1 of the
low-pressure valve to the original
positions, and fix them using a
6 mm and 8 mm wrench.
3 Press [Next].
4 Press [Finish].
After finishing, perform a purge and check
for liquid leakage from any gap between
the pump head and the head holder or
the tubing connection parts.
maintenance log.

3.8 Inspecting (Replacing) the Low-Pressure Valve Rotor and Low-Pressure Valve Stator
Necessary tools
Wrench (6 mm)
Allen wrench (M4)
Necessary parts

Low-Pressure Valve Rotor Consumable part S228-36923
Replacement
Low-Pressure Valve Stator S228-36917-01
part
3
1 Press [LPV Rotor] or [LPV Stator] on the [Replacement Parts Selection] screen.
The [Low pressure valve rotor and stator replacement] screen is displayed.
The [Low pressure valve rotor and stator replacement] screen can also be
displayed by pressing [LPV Rotor] or [LPV Stator] on the replacement parts tab
in the [Maintenance] screen.
2 Replace the low-pressure valve rotor and the low-pressure valve stator.
1 Lower the rinse solution bottle (B)

so that the liquid surface (a) is
lower than the low-pressure valve
(A).

3 Autosampler
3 Use a 6 mm wrench to loosen the

5 male nuts (A) on the housing
and remove the flow line tubing.

screws (A).
5 Remove the low-pressure valve

stator ASSY (A) and ring (B),
taking care not to drop the ring
(B). Place down with the ring side
up.
The low-pressure
valve stator is
divided into the ring
and the low-pressure
valve stator ASSY.
Remove the ring and
housing slowly
because the low-
pressure valve stator
and gasket may drop
from the low-
pressure valve stator
ASSY.

6 Hold the edge (a) of the low-

pressure valve rotor (A) and
remove the rotor.
7 Check for scratches on the

rotating and sliding surfaces (a
and b) of the low-pressure valve
rotor (A) and the low-pressure 3
valve stator (B).
If a scratch is found through
visual inspection, replace with a
new low-pressure valve rotor
and low-pressure valve stator.
Any scratches can

cause wear and tear
in a short period of
time.
8 Disassemble the low-pressure

valve stator ASSY into the low-
pressure valve stator (A), gasket
(B), and housing (C).
9 Immerse the low-pressure valve

rotor (A), low-pressure valve
stator (B), gasket (C), and housing
(D) into a beaker filled with 2-
propanol. and clean for 5 minutes
in an ultrasonic bath.

3 Autosampler
10 Holding the edge of the low-

pressure valve rotor (A), align the
notch (a) of the low-pressure
valve rotor (A) with the alignment
mark (b) of the shaft (B) and
insert horizontally to fit them
together.
As much as possible,
touch only the very
edge of the low-
pressure valve rotor,
and after fitting, use
wiping paper
moistened with 2-
propanol to wipe the
sliding surface that
contacts the low-
pressure valve stator.
11 To assemble the stator ASSY, fit

the gasket (B) and the low-
pressure valve stator (C) to the
housing (A) in that order, then fit
the ring (D).
12 Face the ring side upward, hold

the low-pressure valve stator
ASSY (A) and the ring (B), and
insert into the main unit taking
care not to drop the ring.


screws (A) to the housing (B), and
use an M4 Allen wrench to
gradually tighten each screw in
turn.
Tighten the 3
hexagon socket
screws alternately
60° each, using the
longer side of the
M4 Allen wrench as
a handle to firmly
tighten them at the
end. Do NOT tighten
the hexagon socket
screws unevenly. 3
14 Reattach the flow line tubing of

each low-pressure valve port to
their original positions, and use a
6 mm wrench to tighten the 5
male nuts (A).
3 Press [Next].

3 Autosampler

[Next].
maintenance log.

then press [Finish].

3.9 Replacing the Rinsing Port Cap
3.9 Replacing the Rinsing Port Cap
Necessary parts

For rinse solution containing a
Rinsing Port Cap Consumable
S228-48331-91 highly volatile acid (e.g. formic
(No Hole), 10 Caps part
acid, TFA, acetic acid)
For anything other than the above
(However, if cross contamination is
Rinsing Port Cap Consumable
S228-48331-92 a problem even with the above
(with Hole), 10 Caps part
rinse solution, use the rinsing port
cap with hole.)
3
1 Perform "Z-Home" to move the needle to the center.
"8.2 Z-Home" P.157
2 Press (Power button) to turn OFF.

4 Remove the rinsing port cover (A) from

the rinsing port (B).
5 Remove the cap (B) fitted to the rear

of the rinsing port cover (A).
Because the front cap is not

used, there is no need to
replace it.

3 Autosampler
6 Fit a new cap (C) onto the rinsing port cover (A).
Insert the cap all the way.

If it is raised, it may come into contact with the Z mount.
7 Reattach the rinsing port cover (A).
Press the cover all the way

down.
If it is raised, it may come into
contact with the Z mount.
8 Lightly move the Z mount (A) by hand

and check that it does not interfere
with the rinsing port cover (B).

11 Record the replacement history in the maintenance log.

"2.2 Recording Replacement History in the Maintenance Log" P.10

3.10 Replacing the Rack Panel
3.10 Replacing the Rack Panel
Necessary tools
Phillips screwdriver
Necessary parts

Maintenance
Rack Panel S228-56268-41
part
1 Remove the sample racks (A).

3
2 Use a Phillips screwdriver to remove

the 2 screws (C) that secure the rack
panel (B) and the L-shaped sheet metal
part. Then remove the rack panel (B).

3 Autosampler
3 Use the 2 removed screws (C) to fit a

new rack panel (B).
If the replaced sample racks

interfere with the front panel,
finely adjust the rack panel to
the left or right.
4 Return the sample racks to the original

positions.
5 Record the replacement history in the

maintenance log.
"2.2 Recording Replacement History
in the Maintenance Log" P.10
3.11 Replacing the Front Panel
Necessary parts

Maintenance
Front Panel S228-55569-41
part
2 Replace the front panel.



3.12 Replacing the Wiper
3.12 Replacing the Wiper
Necessary parts

Maintenanc
Wiper Rack S228-57561-41 4 racks
e part
Be careful not to injure your fingers with the edge of the rack plate.

3

3 Slide and remove the 4 wipers (A).
4 Slide and attach the 4 wipers (A).

"3.1 Removing/Attaching the Front
Panel" P.12


3 Autosampler
3.13 Cleaning/Inspecting (Replacing) the Suction Filter
Necessary parts

Suction Filter Consumable part S228-45707-91
2-Propanol - -
1 Remove the suction filter (A) from the

suction tubing (B).
2 Place the suction filter into a clean

container with 2-propanol, and clean it
for 5 minutes using an ultrasonic bath.
When using a buffer solution,

replace it with distilled water or
purified water, and after
pumping at least 20 mL into the
instrument, replace it with new
2-propanol. If the buffer
solution is directly replaced with
2-propanol, crystals will be
precipitated, which can cause
damage to the plunger seal or
cause problems with the check
valve.
After using the buffer solution as the mobile phase, do NOT replace with an
organic solvent such as 2-propanol without first replacing with water.
Crystals, etc., that cannot be removed may appear.
System Guide "3.5.2 Precautions When Replacing the Mobile Phase"
3 Insert the suction tubing (B) into the

suction filter (A).

4 Use a syringe to draw rinse solution from the reservoir bottle, and perform a
flow line purge.
System Guide "4.3 Manual Priming for Rinse Solution of the Autosampler"
5 Check for air bubbles in the suction tubing.

If air bubbles are found, follow the above procedure to remove the suction filter, and
replace with a new suction filter.
6 Record the replacement history in the maintenance log if the suction tubing is
replaced.

3 Autosampler
3.14 Rinsing the Flow Line

When the inside of the needle or sample loop is clogged, or the needle surface is dirty,
use mobile phase solution to rinse the inside and outside of the needle.
3.14.1 Rinsing the Needle and Sample Loop
Rinse the needle and the sample loop.
1 Perform an autopurge.
System Guide "3.5.5 Performing an Autopurge"
• Perform reverse rinsing of the flow line if the inside of the needle is still
clogged after performing the autopurge.
• Replace the needle if the needle is still clogged after reverse rinsing of the
flow line.
"3.14.2 Performing Reverse Rinsing of the Flow Line" P.62

"3.3 Replacing the Needle" P.18
3.14.2 Performing Reverse Rinsing of the Flow Line
If any blockage is found in the flow line inside the instrument, connect the inlet tubing
to port 5 of the high-pressure valve, connect the outlet tubing (SUS pipe HP OUT) to
port 6 of the high-pressure valve in the opposite direction, and perform pumping. This
procedure may remove the blockage in the flow line.

3.14 Rinsing the Flow Line

3 Remove the inlet and outlet tubing on the high-pressure valve side of the
instrument as well as the tubing located on the column side.
4 Connect the inlet tubing to port 5 of the high-pressure valve.

3
5 Connect the side of the outlet tubing that was originally connected to the
column to port 6 of the high-pressure valve.
Use a beaker, etc., to collect the mobile phase coming from the other end of the outlet
tubing.
6 Perform pumping of 2-propanol from the solvent delivery module at 2 mL/min

to 5 mL/min.
7 Return the tubing to the original positions.


3 Autosampler
3.15 Replacing the Outlet Tubing
Do NOT frequently bend and straighten in the same place.

If the tubing is repeatedly bent in the same place, the tubing will weaken,
which may cause a rupture or cracks resulting in leakage of solvent.
Do NOT crush or bend tubing at a sharp angle using pliers, etc.

When bending stainless steel tubing, if the circular arc of the bend (bend
radius) is small, the inside shape of the tubing will become deformed, which
may cause a blockage in the tubing or an increase in pressure.
When replacing clogged outlet tubing, it is recommended to first attempt to

remove the blockage by reverse rinsing of the flow line. After reverse rinsing,
if the tubing is still clogged, follow the procedure below to replace with new
tubing.
"3.14.2 Performing Reverse Rinsing of the Flow Line" P.62
Necessary parts

SUS Pipe 0.3 x 600 OUT Consumable part S228-56217-44
SUS Pipe 0.17 x 600 OUT Optional part S228-56217-45
SUS Pipe 0.1 x 600 OUT Optional part S228-53184-91
• The shape of the tubing connection part may differ depending on the column
manufacturer or column type. After the column is replaced, if cross
contamination increases due to any shape difference, or if the peak shape is
affected, replace with new outlet tubing.
• SUS pipe 0.1 x 600 OUT is a flexible pipe that is easily maneuvered. In addition,
the included fitting is a reusable UHPLC fitting. For information on how to
use the UHPLC fitting, refer to the included instruction manual.
1 Perform "Z-Home" to move the needle to the center.

"8.2 Z-Home" P.157


3.15 Replacing the Outlet Tubing
4 Remove the column side of the outlet tubing from the column.
5 Use a 6 mm wrench to remove the nut (A) of the outlet tubing connected to
port 5 of the high-pressure valve.
6 Open the clamp inside the column

oven and remove the outlet tubing.
7 Use a 6 mm wrench to attach new

outlet tubing to port 5 while ensuring
that the outlet tubing passes through
the notch (A) of the plastic cover
located between the port and the
oven, and then return and fix the
outlet tubing to the clamp inside the
column oven as it originally was.
8 Connect the end of the outlet tubing on the column oven side to the column.
System Guide "3.6.1 Attaching the Column to the Column Oven"



4 Pump
4.1 Attaching/Removing the Pump Heads
Necessary tools
Allen wrench (M5)
Syringe
Syringe needle
n Removing the pump heads
The following will explain how to attach and remove the right-side pump head.
1 Open the pump door.
2 While securing the outlet check valve

(A) with a 10 mm wrench (B), loosen
the male nut (C) of the SUS pipe R
outlet check valve
with an 8 mm wrench (D).

3 Loosen the male nut (A) of the SUS

pipe R (B) pressure sensor with an 8
mm wrench, and remove SUS pipe R
(B).
4 Loosen and remove the male nut PEEK

(A) of the inlet tube by hand.
5 Remove the rinsing kit cap (B) from

the rinse solution bottle (A).
6 Attach the syringe needle (C) to the

end of the outlet FEP tubing (E)
(upward U-shape), and use the syringe
(D) to suction out the rinse fluid from
the rinse flow line.
7 Place the removed cap (B) and rinse solution bottle on the reservoir tray on the
top of the instrument.

4 Pump
8 Gradually loosen the 2 hexagonal

socket screws (A) using an Allen
wrench (M5) by alternating between
the left and right screw, and remove
the pump head (B).
Gently pull the pump head

horizontally along the plunger.
The plunger may be damaged
if the pump head is forcefully
removed.
n Attaching the pump head
1 With the arrow on the pump head (a)

pointing up, gradually tighten the 2
hexagonal socket screws (A) using an
Allen wrench (M5) by alternating
between the left and right screw, and
attach the pump head (B).
Gently insert the pump head

The plunger may be damaged
if the pump head is forcefully
inserted.
Tighten the left and right hexagonal socket screws 90° at a time, and then firmly
tighten using the long side of the Allen wrench.
2 Tighten the male nut PEEK (A) of the

inlet tube to the inlet check valve (B)
by hand.

3 Tighten the male nut (A) of the SUS

pipe R (B) pressure sensor with an 8
mm wrench, and attach SUS pipe R (B).
4 While securing the outlet check valve

(A) with a 10 mm wrench (B), tighten
the male nut (C) of the SUS pipe R
outlet check valve
with an 8 mm wrench (D).
4
5 Attach the original rinsing kit cap (B)

to the rinse solution bottle (A), and
then remove the outlet FEP tubing (E)
(upward U-shape) from the rinse
solution bottle.
System Guide "4.1 Replacing the
Rinse Fluid of the Pump Automatic
Rinsing Kit"
6 Attach the syringe needle (C) to the

end of the FEP tubing (E)(upward U-shape), and use the syringe (D) to suction
out the 10% 2-propanol solution from the rinse flow line.
7 Next, return the rinsing kit to the reservoir tray.

4 Pump
8 Close the pump door.
4.2 Replacing the Plunger Seal

A plunger seal is attached inside the left and right pump heads. The following section
will explain the method for replacing the plunger seal on the right side (when viewed
from the front).
Necessary tools
Allen wrench (M5)
Necessary parts
Part Name Part Type Part No. Necessary Quantity

Plunger Seal Consumable part S228-35146 1
1 Press [Plunger Seal(R)] on the [Replacement Parts Selection] screen.

"2.1 Displaying the Replaced Part Items" P.7
The [Plunger seal replacement] screen is displayed.
2 Select [Right], and then press [Next].

The pump operates and the plunger on
the right side moves toward the back of
the instrument. The next screen is
displayed.
3 Remove the pump head.

"4.1 Attaching/Removing the Pump Heads" P.66
Remove the pump head after the [Pump] display turns off.

4 Press [Next].
5 Replace the plunger seal.
1 Vertically insert the end of the

seal tool (A) with a stepped side
(a) into the plunger seal (B).
2 Pull out the seal tool (A) vertically

to remove the plunger seal (B)
from the pump head (C). 4
3 Place the pump head into a clean beaker containing 2-propanol, and clean it for
10 minutes using an ultrasonic bath.
4 Use a piece of wiping paper

soaked in 2-propanol to wipe the
plunger attachment portion inside
the pump head (a).
• Airtightness cannot be maintained if part of the seal, etc., is stuck

to the pump head where the plunger seal attaches.
• Replace the pump head if there are scratches on the pump head
where the plunger seal attaches.

4 Pump
5 Immerse the plunger seal in a clean container with 2-propanol.
6 Insert the end of the seal tool (A)

with no unevenness (a) into the
plunger seal (B), and vertically
insert it into the pump head
where the plunger seal attaches
(b).
Be careful not to
scratch the inner
surface of the
plunger seal with the
seal tool.
7 Pull out the seal tool (A) vertically.
6 Press [Next].
7 Attach the pump head.

"4.1 Attaching/Removing the Pump
Heads" P.66
8 Press [Next].
value is reset.
maintenance log.

then press [Next].

10 Select the mobile phase that will be

used for purging, and then manually
purge the pump.
When proceeding to replace the

plunger seal on the left side
(when viewed from the front),
press [Stop] and return to
Procedure 1, and then, after
replacing the plunger seal in
the same procedure, perform a
purge.
Operation Guide "5.1.1 Pump"
11 Insert the syringe with needle (B) into

the mobile phase drain tube (A)
marked with "P" (a). 4
12 Open the pump door, rotate the drain

valve knob (A) 90 degrees counter-
clockwise, and open the drain valve.
13 Aspirate the mobile phase with the syringe.

The criterion value for the aspirated volume is 10 mL.

4 Pump
14 Close the drain valve by rotating the

drain valve knob (A) clockwise until
the rotation stops.
16 Remove the syringe.
17 Press [Next].
18 Press [Start].
The autopurge starts.
After autopurge finishes, the next screen is
displayed automatically.
19 After replacing the plunger seal, perform a check.

• Is the pumping (pressure) stable?
• Is fluid leaking from the gap between the pump head and the head holder?
If the above occurs even after replacing the plunger seal, it is possible that there
are scratches on the surface of the plunger. If there are scratches on the plunger,
replace it with a new plunger.
"4.3 Cleaning/Inspecting (Replacing) the Plunger and Diaphragm" P.76

20 Press [Finish].

4 Pump
4.3 Cleaning/Inspecting (Replacing) the Plunger and Diaphragm

A plunger and a diaphragm are attached to the left and right pump body. The following
section will explain the method for cleaning/inspecting (replacing) the plunger and the
diaphragm on the right side (when viewed from the front).
When replacing the plunger, be sure to also replace the plunger seal and the diaphragm.
Necessary tools
Allen wrench (M4 and M5)
Box driver (Part No.: S228-28767-91)
Diaphragm jig (Part No.: S228-54852)
Necessary parts

Maintenance
Plunger Holder ASSY S228-35281-97 1
part
1 Press [Plunger&Diaphragm] on the [Replacement Parts Selection] screen.

The [Plunger and diaphragm replacement] screen is displayed.
2 Press [Right], and then press [Next].

The pump operates and the plunger on
the right side moves toward the back of
the instrument. The next screen is
displayed.
3 Remove the pump head.

"4.1 Attaching/Removing the Pump
Heads" P.66
Remove the pump head after the [Pump] display turns off.

4 Remove the head holder.
1 Loosen the head holder top and

bottom fittings (A, B), and
remove the rinse tube.
2 Gradually loosen the 2 hexagonal

socket screws (B) of the head
holder (A) using an Allen wrench
(M4) by alternating between the
left and right screw, and remove
the head holder.
4
n Head holder is difficult to remove
Insert into the head holder M4 bolt hole

(a) the 2 hexagonal socket screws (B) that
were removed, and tighten the left and
right sides gradually using an Allen wrench
(M4) (D).
The head holder (A) can be loosened by
pushing the pump body (C) using the 2
hexagonal socket screws (B).
Gently pull the head holder

If the head holder is not
removed carefully, the plunger
may become damaged.

4 Pump
5 Press [Next].
6 Cleaning/Inspecting (Replacing) the Plunger and Diaphragm.
1 Use a wiping paper soaked with

2-propanol to remove any portion
of the seal or dirt attached to the
plunger (A).
If part of the seal

remains attached,
airtightness cannot
be maintained.
2 Check that there are no visible scratches on the plunger (A) or the diaphragm (B).
• If scratches are found through visual inspection, replace with a new plunger.
Proceed to Procedure 6-3.
• If no scratches are found through visual inspection, proceed to Procedure 7.
Do NOT allow foreign particles or crystals into the head holder.

Foreign particles from the mobile phase solution or crystals from the buffer
solution getting into the head holder may cause scratches on the surface of
the plunger.

3 Insert the box driver (A) into the

plunger holder (B), then rotate
counter-clockwise to remove it.
Gently insert the box driver

into the plunger.
The plunger may become
damaged if the box driver is
forcefully inserted.
4 Attach a new diaphragm (B) to

the new plunger holder (A) using
the diaphragm jig (C).
Confirm the
direction of the
diaphragm when
attaching. 4
5 After applying the grease (black)

provided with the plunger holder
ASSY to the rear end surface of
the plunger (a), insert the plunger
holder (A) to which the
diaphragm was attached in
Procedure 4 into the pump body.
• Be careful not to get any grease on the diaphragm or the body.

• Attach the diaphragm so that it will not become detached from the
plunger holder. If the diaphragm becomes detached, wipe away any
grease and perform from Procedure 6-4 again.

4 Pump
6 Tighten the plunger holder (B)

using the box driver (A).
7 Next, use a piece of wiping paper
soaked in 2-propanol to wipe and
clean the plunger surface.
7 Replace the plunger seal.

Press [Stop] and replace the plunger seal following Procedure 1 through 9 in "4.2
Replacing the Plunger Seal" P.70. After pressing [Stop] on the screen for Procedure 10
in "4.2 Replacing the Plunger Seal" P.70, proceed to Procedure 2 in "4.3
Cleaning/Inspecting (Replacing) the Plunger and Diaphragm" P.76, and then proceed to
Procedure 8.
"4.2 Replacing the Plunger Seal" P.70
8 Press [Next].

9 Attach the head holder and the pump head.
1 Gradually tighten the 2 hexagonal

socket screws (B) using an Allen
wrench (M4) by alternating
between the left and right screw,
and attach the head holder (A).
2 Grip the end of the plunger with

two fingers and adjust the gap
(C) between the plunger (A) and
the hole in the head holder (B) so
that it is uniform. Then, wipe off
the plunger surface with clean
wiping paper soaked in 2- 4
propanol, being careful not to tilt
the plunger.
Move the plunger

gently. Moving it too
abruptly could
scratch the plunger
surface or cause it to
break off.
3 Attach the head holder.
Tighten the left and right hexagonal socket screws 90 ° at a time,

and then firmly tighten using the long side of the Allen wrench.
"4.1 Attaching/Removing the Pump Heads" P.66
Gently push in the head holder horizontally along the plunger.

The plunger may become damaged if the head holder is forcefully inserted.

4 Pump
10 Press [Next].

[Next].

12 Check the diaphragm assembly.
1 Insert the syringe needle (B) into

the end of the accessory syringe
(A).
2 Attach the accessory flangeless

fitting ASSY (B) to the end of the
syringe needle (A), and rotate it
clockwise to attach to the rinse
fluid outlet (a) at the top of the
head holder.
3 Cover the rinse fluid inlet (a) at

the bottom of the head holder
with a finger.

4 Pump
4 Press and release the plunger of

the syringe.
If there is no air leak, the plunger
of the syringe will return to its
original position.
If there is an air
leak, check the
connection portion
between the
flangeless fitting
ASSY and the head
holder. If there is no
problem, disassemble
it again to check the
attachment of the
diaphragm.
5 Loosen the fitting (A) at the top

of the head holder and remove
the syringe.
6 Loosen the head holder top and

bottom fittings (A, B), and attach
the original rinse tube.
7 Reattach the SUS tubing and the

inlet tube.
"Attaching the pump
head" P.68, Procedure 2
to 4

13 Press [Next].

used for purging, and then press
[Next].

plunger and diaphragm on the
left side, press [Stop] and
perform from Procedure 1 again 4
without performing a purge.
15 Press [Start].
Purging starts.
After purging finishes, the next screen is
16 After replacing the plunger seal, perform a check.

• Is the pumping (pressure) stable?
• Is fluid leaking from the gap between the pump head and the head holder?
If the above occurs even after replacing the plunger seal, it is possible that there
are scratches on the surface of the plunger. If there are scratches on the plunger,
replace it with a new plunger.
"4.3 Cleaning/Inspecting (Replacing) the Plunger and Diaphragm" P.76

4 Pump
17 Press [Finish].
maintenance log.
4.4 Rinsing the Check Valve

This section will explain how to pump 2-propanol to rinse the check valve.
Necessary tools
Wrench (8 mm)
Necessary parts

Replacement
Resistor Tube S228-57908-41 1
part
1 Replace the solvent in reservoir bottle A with 2-propanol.
When using a buffer solution in the reservoir bottle, replace with distilled water
or purified water, and after pumping at least 20 mL into the instrument, replace
with new 2-propanol. If the buffer solution is directly replaced with 2-propanol,
crystals will be precipitated, which can cause damage to the plunger seal or
cause problems with the check valve.

4.4 Rinsing the Check Valve
2 Open the column oven door.
3 Loosen the male nut (A) of the column

inlet with an 8 mm wrench, loosen the
male nut (C) of the column outlet by
hand, and remove the column (B) from
the flow line.
4 Attach the male nut (A) of the column

inlet that was removed to the resistor 4
tube (D), and tighten it with an 8 mm
wrench.
5 Attach the other male nut (C) of the

column outlet to the resistor tube (D),
and tighten it by hand.

4 Pump
6 From the Main Analysis screen, set so that pumping is only performed for port A.
• If the pumping mode is [ISO], set the

mobile phase port to [A].
• If the pumping mode is [GRAD.], set the

concentration for [B], [C], and [D] to
"0".
7 Set the flow rate to "2 mL/min".
8 Press [Pump].
2-propanol is pumped at a rate of
2 mL/min.
Perform pumping for at least 1 hour.

4.5 Performing an Ultrasonic Cleaning of the Check Valve
9 Loosen the male nut (A) of the resistor

tube inlet with an 8 mm wrench,
loosen the male nut (C) of the resistor
tube outlet by hand, and remove the
resistor tube (B) from the flow line.
10 Attach the male nut (A) of the resistor

tube inlet that was removed to the
column (D) with an 8 mm wrench, and
tighten it with the 8 mm wrench.
11 Attach the other male nut (C) of the

resistor tube outlet to the resistor tube
(D), and tighten it by hand.
12 Close the column oven door.

4
13 Replace the solvent in reservoir bottle A with the original mobile phase.
14 Insert the suction filter into reservoir bottle A.
4.5 Performing an Ultrasonic Cleaning of the Check Valve

A check valve is attached to the left and right pump heads. The following section will
explain the method for performing an ultrasonic cleaning of the check valve on the right
side (when viewed from the front).
Necessary tools
1 Remove the SUS tubing and the inlet tube.

"Removing the pump heads" P.66, Procedure 1 to 4

4 Pump
2 Remove the check valve.
Never disassemble the check valve.

Performance cannot be guaranteed if disassembled.

the inlet check valve (A) and the
outlet check valve (B).
2 Immerse the inlet check valve and

the outlet check valve into 2-
propanol, and rinse for 5 minutes
using an ultrasonic bath.
3 Rotate the inlet check valve (A)

and the outlet check valve (B)
clockwise to attach them to the
pump head, and after tightening
by hand, use a 10 mm wrench to
tighten them 90 °.
3 Attach the SUS tubing and the inlet tube.

"Attaching the pump head" P.68, Procedure 2 to 4

4.6 Inspecting (Replacing) the Check Valve

A check valve is attached to the left and right pump heads. The following section will
explain the method for inspecting (replacing) the check valve on the right side (when
viewed from the front).
Necessary tools
Necessary parts

Maintenance
Inlet Check Valve ASSY S228-48249-96 1
part
Maintenance
Outlet Check Valve ASSY S228-45705-91 1
part
1 Check the pumping stability.

Integrity Guide "2.4.2 Pump Solvent ■Delivery Stability Check" 4
If the result of the system check is "Pass", it is considered normal.
If the result of the system check is "Fail", perform "4.4 Rinsing the Check Valve" P.86
or "4.5 Performing an Ultrasonic Cleaning of the Check Valve" P.89, and then check the
pumping stability again. If the pumping (pressure) is still unstable, proceed to the
following procedure.
2 Press [Check Valve(R)] on the [Replacement Parts Selection] screen.

The [Check valve replacement] screen is displayed.
3 Select [Right], and then press [Next].

4 Remove the SUS tubing and the inlet tube.

"Removing the pump heads" P.66, Procedure 1 to 4

4 Pump
5 Press [Next].
6 Replace the check valve.

the inlet check valve (A) and the
outlet check valve (B).
Never disassemble the check

valve.
Performance cannot be
guaranteed if disassembled.
2 Attach a new inlet check valve (A)

and a new outlet check valve (B)
to the pump head, and after
tightening by hand, use a 10 mm
wrench to tighten them 90º.

7 Press [Next].
8 Attach the SUS tubing and the inlet tube.

"Attaching the pump head" P.68, Procedure 2 to 4
9 Press [Next].
4

[Next].

4 Pump

used for purging, and then press
[Next].

check valve on the left side
(when viewed from the front),
press [Stop] and return to
Procedure 1, and then, after
replacing the check valve in the
same procedure, perform a
purge.
12 Press [Start].
Purging starts.
After purging finishes, the next screen is
13 Press [Finish].
maintenance log.

4.7 Inspecting the Line Filter
4.7 Inspecting the Line Filter
Necessary tools
2 Secure the line filter (A) using a 10 mm

wrench (B), and loosen the male nut 4
(C) of the SUS tubing using an 8 mm
wrench (D) to remove it.
3 Pour water into the reservoir bottle,

and from the Main Analysis screen, set
the flow rate to 1 mL/min.
4 Press [Pump].
Place a piece of wiping paper

onto the leak tray just below
the line filter, and collect the
water that comes out from the
line filter.

4 Pump
5 Check the pressure.

• If the pressure is 0.3 MPa or less, it is considered normal.
• If the pressure is more than 0.3 MPa, replace the line filter.
"4.8 Replacing the Line Filter" P.97
• Rinsing method when a ghost peak is generated

When a mobile phase such as trifluoroacetic acid is used, a ghost peak may
be generated from the line filter. In this case, perform the following procedure
to rinse the line filter.
Deliver a 17% phosphoric acid solution at a flow rate of 1 mL/min for about
1
30 minutes. (Pumping pressure: 1 MPa to 5 MPa)
Deliver purified water at a flow rate of 9 mL/min for about 30 minutes.
2
(Pumping pressure: 1 MPa to 5 MPa)
• Precaution when using a mass spectrometry system

When a mass spectrometry system is used, deliver a 50 % methanol aqueous
solution at about 10 MPa for about 30 minutes for background reduction.
6 Attach the male nut (B) of the SUS

tubing to the line filter (A) by hand.

wrench (B) and tighten the male nut
wrench (D).

4.8 Replacing the Line Filter
4.8 Replacing the Line Filter
Necessary tools
Necessary parts

Line Filter Consumable part S228-35871-96 1

wrench (B), and loosen the male nut
wrench (D) to remove it.

4 Pump
3 Loosen the line filter (A) with a 10 mm

wrench to remove it.
4 Use a cotton swab, etc., to wipe away

any dirt from the line filter port (a).
5 Attach the new line filter (A) to the

line filter port (a), and tighten it
45° using a 10 mm wrench.
6 Attach the male nut (C) of the SUS

tubing to the line filter (A) by hand.

wrench (B) and tighten the male nut
wrench (D).


maintenance log.
Necessary parts

Suction Filter Consumable part S228-45707-91 1
1 Pull the suction filter (A) from the 4

suction tubing (B).
2 Place the suction filter into a clean

container with 2-propanol, and clean it
for 5 minutes using an ultrasonic bath.
When using a buffer solution,

replace it with distilled water or
purified water, and after
pumping at least 20 mL into the
instrument, replace it with new
2-propanol. If the buffer
solution is directly replaced with
2-propanol, crystals will be
precipitated, which can cause
damage to the plunger seal or
cause problems with the check
valve.

4 Pump
3 Insert the suction tubing (A) into the

suction filter (B).
4 Replace the solvent in reservoir bottle

A with water, and place the suction
tubing back into the bottle.
5 Press [Purge] on the main analysis

screen.
The [Auto Purge] screen is displayed.
6 Select [1st] only, and set [A] to

[10 min].
7 Press [Start].
Purging starts.
8 Check for air bubbles inside the suction tubing.

Replace the suction filter if air bubbles are present.


4.10 Replacing the Drain Valve ASSY
4.10 Replacing the Drain Valve ASSY
Necessary tools
Phillips screwdriver
Necessary parts

Replacement
Drain Valve ASSY S228-45574-95 1
part
2 Rotate the drain valve ASSY (A)

counter-clockwise at least 3 complete
rotations and pull it off straight.
Check that there are no 4

scratches or dirt in the hole
after removal.

4 Pump
3 Wet the seal portion (a) of the new

drain valve ASSY (A) with 2-propanol,
etc., and insert it into the hole.
If the drain valve ASSY is

forcefully inserted or is not
inserted straight, the seal
portion may become deformed,
resulting in leakage.
4 Tighten the drain valve ASSY (A)

clockwise.
5 After tightening the drain valve ASSY

(A), attach the knob (B) using the
screw (C) so that it is vertical, and then
attach the cap (D).

4.11 Cleaning of the Automatic Rinsing Kit

maintenance log.
4.11 Cleaning of the Automatic Rinsing Kit

If there is white dirt inside the rinse solution bottle of the automatic rinsing kit, remove
the dirt according to the following procedure.
Necessary parts

Replacement
Rinse Flow Line ASSY
part
S228-56201-41 1 4
If the 10% 2-propanol water in the rinse solution bottle is dirty, replace with
new solution. Especially when getting dirty significantly, replace with new
solution once a day. Even if it does not appear dirty, replace with new solution
once a week.
1 Remove the rinse cap of the automatic rinsing kit from the rinse solution bottle.
2 Pour distilled water into the rinse solution bottle and clean the inside of the
rinse solution bottle using a brush, etc.
3 Pour distilled water into the rinse solution bottle and rinse the inside of the
rinse solution bottle for about 5 minutes using an ultrasonic bath.
4 Pour new 10% 2-propanol water into the rinse solution bottle, and return the
cap and rinse solution bottle back to their original positions.
System Guide "4.1 Replacing the Rinse Fluid of the Pump Automatic Rinsing Kit"
5 Replace the rinse tube if the inside is dirty.

5 UV Detector
5.1 Removing/Attaching the Flow Cell
n Removing the flow cell
1 Open the controller panel.
2 After removing the tubing from the

clamps (2 locations) (C), loosen the
coupling setscrews (A) and remove the
2 couplings (B) on the end of the flow
cell inlet tubing and the flow cell
outlet tubing.
3 Remove the temperature controlled

flow cell connector (A).

4 Loosen the 2 cell retaining screws (A)

and remove the flow cell (B).
If the cell retaining screws are

too tight to be loosened, use a
flathead screwdriver to loosen
them.
n Attaching the flow cell
1 Tighten the 2 cell retaining screws (A)

and attach the flow cell (B).
When tightening the 2 cell

retaining screws, secure the cell
to the instrument by pressing
on it, and alternate between
tightening the 2 screws. If only
one side is fully tightened, the
cell may become uneven.
5
2 Attach the temperature controlled
flow cell connector (A) to the
temperature controlled flow cell
connector socket (a).

5 UV Detector
3 Connect the tubing (A) from the

column and the flow cell inlet tubing
(blue) (B) if disconnected. Also connect
the tubing from the flow cell outlet
tubing to the drain if disconnected.
4 After attaching the tubing to the

clamps (2 locations) (C), tighten the
coupling setscrews (A) and affix the 2
couplings (B) on the end of the flow
outlet tubing.
5 Close the controller panel.

5.2 Flow Cell Inspection / Simple Cleaning

Inspect the flow cell if there is an excessive amount of noise or drift. If air bubbles or
dirt is present after inspection, perform simple cleaning.
Necessary parts

Syringe Optional part S046-00017-01 -
Syringe Adapter Optional part S228-15672-91 -
2-Propanol - - For cleaning
1 Remove the flow cell.

"Removing the flow cell" P.104
Leave the tubing connected.
2 After setting the flow rate to

1 mL/min, press [Pump] and perform
pumping.
3 While the mobile phase solution is

flowing, view the inside of the flow
cell (A) from the lens side (a) to check
for air bubbles or dirt.
• If air bubbles or dirt is present inside the
flow cell, perform simple cleaning.
Proceed to the next procedure.
• If no air bubbles or dirt is present inside
the flow cell, proceed to Procedure 5.

5 UV Detector
4 Perform simple cleaning.
1 Loosen the male nut (A) on the

column side, and remove the flow
cell inlet tubing (blue) (B).
2 Connect the syringe adapter (A)

to the flow cell inlet tubing (blue)
(B).
3 If a water-based solvent
(especially solvents containing
salt) is used as the mobile phase
for analysis, attach the syringe (B)
with water to the syringe adapter
(A), and slowly push the plunger
(C).
The solvent in the flow cell is
displaced by the water.
If a water-based solvent (especially solvents containing salt) is not used as the
mobile phase for analysis, proceed to the next procedure.
If an organic solvent and salt are mixed, precipitate is generated,

which can cause the tubes to clog.

4 Remove the syringe (B) from the

syringe adapter (A).
5 Attach the syringe (B) with 2-

propanol to the syringe adapter
(C).
The fluid from the syringe flows
into the flow cell and any dirt is
removed.
6 Loosen the male nut (A), and

remove the syringe adapter (B).
5 Attach the flow cell.

"Attaching the flow cell" P.105

5 UV Detector
5.3 Disassembling and Cleaning the Flow Cell

If dirt and air bubbles cannot be removed by performing a simple cleaning, disassemble
and clean the flow cell. Also, disassembling and cleaning the flow cell is recommended
every 2 years.
Necessary tools
Flathead screwdriver (Part No.: S086-10504-18)
Plastic tweezers (with no dirt on the end)
Air blower (for the lens) (Part No.: S086-78801-02)
If plastic tweezers are not available, use some kind of alternative item such as
a toothpick for removing. In this case, use something soft so that it does not
scratch the lens or the cell window.
Necessary parts

Gasket (Set of 2)
Be sure to replace when
Gasket Consumable part S228-35097-95
disassembling and cleaning the
flow cell. *1
2-Propanol - - -
(Mobile Phase
Solution Used for - - -
Analysis)
*1 If use of the gaskets is continued without replacing, fluid may begin to leak.
1 Press [Flow cell] on the [Replacement Parts Selection] screen.

The [Flow cell replacement] screen is displayed.


3 Remove the lens, cell window, and cell gasket.

Remove the flow cell outlet
tubing in the same way.
2 Remove the left and right

setscrews (A) using a flathead
screwdriver.
3 Remove the lens (A), cell window

(B), and gasket (C) using plastic
tweezers.
4 Press [Next].

5 UV Detector
5 Immerse the lens and cell window in 2-propanol, and rinse for 5 minutes in an
ultrasonic bath.
6 Press [Next].
If dirt on the lens cannot be removed,
press [Stop] to suspend work, and then
proceed to "5.4 Replacing a Flow Cell
Part" P.115.
7 Attach the lens, cell window, and 2 new cell gaskets.

soaked in 2-propanol to remove
dirt inside the cell housing (a).
2 If dust is attached to the lens, cell

window, or gaskets, remove it
using an air blower (for the lens)
while securing the lens, cell
window, or gaskets with plastic
tweezers so as not to be blown
away by the air.
If an air blower (for the lens) is not available, use a piece of wiping paper
soaked in 2-propanol to remove the dust.

3 Attach the new gaskets (A) and

the lens (B) or the cell window (C)
in that order using plastic
tweezers, and attach the setscrew
(D) using a flathead screwdriver.
Do NOT attach the

lens and cell window
backwards.
Performance may be
affected.
Attach the lens so that the convex surface is facing outward.

It may be damaged if attached facing the wrong direction.

(B). Connect the flow cell outlet
tubing to the tubing to the drain.
5 Attach the syringe (B) with mobile

phase solution for analysis to the
syringe adapter (A), and slowly
push the plunger (C) for a fluid
leak.
If there is a fluid
leak, rinse the lenses
and the cell window
again, and then
tighten the setscrews
again so that they
are not loose.

5 UV Detector


9 Press [Next].
10 Select the button for the replaced part,

and then press [Finish].

5.4 Replacing a Flow Cell Part

It is necessary to replace the lens or the cell window if dirt on the lens or cell window
cannot be removed after disassembling and cleaning the flow cell. The gaskets should
be replaced at the same time.
Also, it is recommended to replace the lenses and the cell window every 2 years.
Necessary tools
a toothpick for removing.
In this case, use something soft so that it does not scratch the lens or the cell
window.
Necessary parts

Gasket (Set of 2)
flow cell. *1
Lens
Maintenance
S228-14572 - 5
part
Maintenance
Cell Window S228-18058 -
part
2-Propanol - - -



5 UV Detector
3 Remove the lens, cell window, and cell gasket.


screwdriver.
3 Remove the lens (A), cell window

(B), and gaskets (C) using plastic
tweezers.

4 Press [Next].
5 Attach the lens, cell window, and 2 new cell gaskets.

2 If dust is attached to the lens, cell 5

window, or new gaskets, remove
it using an air blower (for the
lens) while securing the lens, cell
window, or new gaskets with
plastic tweezers so as not to be
blown away by the air.

5 UV Detector

the lens (B) or the cell window (C)
in that order using plastic
tweezers, and attach the setscrew
(D) using a flathead screwdriver.
Do NOT attach the

lens and cell window
backwards.
Performance may be
affected.



push the plunger (C) to check for
a fluid leak.
If there is a fluid
and the cell window
again, and then
again so that they
are not loose.



7 Press [Next].


5 UV Detector
5.5 Replacing the D2 Lamp
Necessary tools
Phillips screwdriver (#2)
Necessary parts

D2 Lamp Consumable part S228-55626 -
As the D2 Lamp used in this device approaches the end of its service life, the
light intensity will decrease and baseline noise will increase. Replace with a new
lamp using 2000 hours as a replacement guideline.
D2 Lamp warranty period

The D2 Lamp warranty period is 2000 hours of use or 1 year after purchase, whichever
comes first.
1 Press [D2 Lamp] on the [Replacement Parts Selection] screen.

The [D2 lamp replacement] screen is displayed.

The Confirmation screen opens. Press [OK]
to turn the instrument power OFF.

3 Replace the D2 lamp.
Because the D2 lamp may be hot, do NOT operate until the lamp has been
OFF for about 30 minutes.
There is a risk of being burned. Be sure to wait at least 30 minutes after the
lamp has been turned OFF before replacing the lamp.
Do NOT shake the lamp or subject the lamp to strong impacts.

The lamp may break.
Do NOT directly touch the glass portion with your fingers when replacing the
lamp.
Performance may be affected.
2 Loosen the lamp cover mounting

screw (A), and remove the lamp
cover (B).

5 UV Detector
3 Remove the connector (A).
4 Loosen the two D2 lamp setscrews

(B) using a Phillips screwdriver,
and remove the D2 lamp (C).
5 Align the notch (a) on the new

D2 lamp (C) with the positioning
pin, and alternate between
tightening the two D2 lamp
setscrews (B) using the Phillip's
screwdriver.
If dirt is attached to
the lamp, use a piece
of lens paper with
ethanol to wipe it
away. Performance
may be affected if it
is dirty.
6 Attach the connector (A).
7 Insert the lamp cover into the

frame slit (a).
8 Tighten the lamp cover mounting

screw (A) to attach the lamp
cover (B).

the [D2 lamp replacement] screen.
5 Select [Next].
value is reset.
maintenance log.
6 Check that the usage value has reset,

7 Perform wavelength accuracy

calibration.
Integrity Guide "3.2.5 UV Detector
■Wavelength Accuracy Calibration"

6 PDA Detector
n Removing the flow cell
2 After removing the tubing from the

clamps (2 locations) (C), loosen the
coupling setscrews (A) and remove the
2 couplings (B) on the end of the flow
outlet tubing.
3 Remove the temperature controlled

flow cell connector (A).

4 Loosen the 2 cell retaining screws (A)

and remove the flow cell (B).
If the cell retaining screws are

too tight to be loosened, use a
flathead screwdriver to loosen
them.
n Attaching the flow cell
1 Tighten the 2 cell retaining screws (A)

and attach the flow cell (B).
When tightening the 2 cell

retaining screws, secure the cell
to the instrument by pressing
on it, and alternate between
tightening the 2 screws. If only
one side is fully tightened, the
cell may become uneven.
2 Attach the temperature controlled

flow cell connector (A) to the
temperature controlled flow cell
connector socket (a).
6

6 PDA Detector
3 Connect the tubing (A) from the

column and the flow cell inlet tubing
(blue) (B) if disconnected. Also connect
the tubing from the flow cell outlet
tubing to the drain if disconnected.
4 After attaching the tubing to the

clamps (2 locations) (C), tighten the
coupling setscrews (A) and affix the 2
couplings (B) on the end of the flow
outlet tubing.


Inspect the flow cell if there is an excessive amount of noise or drift. If air bubbles or
dirt is present after inspection, perform simple cleaning.
Necessary parts

Syringe Optional part S046-00017-01 -
Syringe Adapter Optional part S228-15672-91 -
2-Propanol - - For cleaning

2 After setting the flow rate to

1 mL/min, press [Pump] and perform
pumping.
3 While the mobile phase solution is

flowing, view the inside of the flow
6
cell (A) from the direction of the arrow
to check for air bubbles or dirt.
• If air bubbles or dirt is present inside the
flow cell, perform simple cleaning.
Proceed to the next procedure.
• If no air bubbles or dirt is present inside
the flow cell, proceed to Procedure 5.

6 PDA Detector
4 Perform simple cleaning.


(B).
3 If a water-based solvent
(especially solvents containing
salt) is used as the mobile phase
for analysis, attach the syringe (B)
with water to the syringe adapter
(C).
The solvent in the flow cell is
displaced by the water.
If a water-based solvent (especially solvents containing salt) is not used as the

mobile phase for analysis, proceed to the next procedure.
If an organic solvent and salt are mixed, precipitate is generated,

which can cause the tubes to clog.

4 Remove the syringe (B) from the

syringe adapter (A).
5 Attach the syringe (B) with 2-

propanol to the syringe adapter
(C).
The fluid from the syringe flows
into the flow cell and any dirt is
removed.



6 PDA Detector

If dirt and air bubbles cannot be removed by performing a simple cleaning, disassemble
and clean the flow cell.
Also, disassembling and cleaning the flow cell is recommended every 2 years.
Necessary tools
In this case, use something soft so that it does not scratch the lens.
Necessary parts

Gasket (Set of 2)
flow cell. *1
2-Propanol - - -
(Mobile Phase
Solution Used for - - -
Analysis)



3 Remove the lenses and cell gaskets.


screwdriver.
3 Remove the lens (A) and gasket

(B) using plastic tweezers.
6
4 Press [Next].

6 PDA Detector
5 Immerse the lenses in 2-propanol, and rinse for 5 minutes in an ultrasonic bath.
6 Press [Next].
If dirt on the lens cannot be removed,
press [Stop] to suspend work, and then
proceed to "6.4 Replacing a Flow Cell
Part" P.135.
7 Attach the 2 lenses and 2 new cell gaskets.

2 If dust is attached to the lenses or

gaskets, remove it using an air
blower (for the lens) while
securing the lenses or gaskets
with plastic tweezers so as not to
be blown away by the air.


lenses (B) in that order using
plastic tweezers, and attach the
setscrews (C) using a flathead
screwdriver.



a fluid leak.
If there is a fluid
again, and then
again so that they
are not loose.

6 PDA Detector


9 Press [Next].



It is necessary to replace the lens if dirt on the lens cannot be removed after
disassembling and cleaning the flow cell. The gaskets should be replaced at the same
time.
Also, it is recommended to replace the lenses every 2 years.
Necessary tools
In this case, use something soft so that it does not scratch the lens.
Necessary parts

Gasket (Set of 2)
flow cell. *1
Maintenance
Lens S228-14572 -
part
2-Propanol - - -

6


6 PDA Detector
3 Remove the lenses and cell gaskets.


screwdriver.
3 Remove the lens (A) and gasket

(B) using plastic tweezers.

4 Press [Next].
5 Attach the 2 lenses and 2 new cell gaskets.

2 If dust is attached to the lenses or

new gaskets, remove it using an
air blower (for the lens) while
securing the lenses or new
gaskets with plastic tweezers so as
not to be blown away by the air.
6

6 PDA Detector

lenses (B) in that order using
plastic tweezers, and attach the
setscrews (C) using a flathead
screwdriver.



a fluid leak.
If there is a fluid
again, and then
again so that they
are not loose.



7 Press [Next].
6

6 PDA Detector
Necessary tools
Necessary parts

D2 Lamp PDA Consumable part S228-55626-01 -
As the D2 Lamp used in this device approaches the end of its service life, the
light intensity will decrease and baseline noise will increase. Replace with a new
lamp using 2000 hours as a replacement guideline.
D2 Lamp warranty period

The D2 Lamp warranty period is 2000 hours of use or 1 year after purchase, whichever
comes first.
1 Press [D2 Lamp] on the [Replacement Parts Selection] screen.

The [D2 lamp replacement] screen is displayed.


3 Replace the D2 lamp.
Because the D2 lamp is hot, do NOT operate until the lamp has been OFF for
about 30 minutes.

The lamp may break.
lamp.
2 After removing the tubing from

the clamp (C), loosen the lamp 6
cover mounting screw (A) and
remove the lamp cover (B).

6 PDA Detector
4 Loosen the two D2 lamp setscrews

(B) using a Phillips screwdriver,
and remove the D2 lamp (C).
5 Align the notch (a) on the new

D2 lamp (C) with the positioning
pin, and alternate between
tightening the two D2 lamp
setscrews (B) using a Phillips
screwdriver.
If dirt is attached to
the lamp, use a piece
of lens paper with
ethanol to wipe it
away. Performance
may be affected if it
is dirty.
7 Insert the lamp cover into the

frame slit (a).

screw (A) and attach the lamp
cover (B), and then attach the
tubing to the clamp (C).

the [D2 lamp replacement] screen.
5 Select [Next].
value is reset.
maintenance log.

and then press [Finish]. 6
7 Perform exposure time calibration.

Integrity Guide "3.2.6 PDA Detector
■Exposure Time / W Lamp Voltage
Setting"
8 Perform wavelength accuracy

calibration.
Integrity Guide "3.2.6 PDA Detector ■Wavelength Accuracy Calibration"

6 PDA Detector
6.6 Replacing the W Lamp (Optional Part)

When a W lamp (optional part) is used, replace the W lamp according to the following
procedure.
Necessary tools
Plate W (Part No.: S228-34397)
W lamp kit (Part No.: S228-57110-41) accessory
Necessary parts

W Lamp Consumable part S062-65012-04 -
1 Press [W Lamp] on the [Replacement Parts Selection] screen.

The [W lamp replacement] screen is displayed.


3 Replace the W lamp.
Because the W lamp is hot, do NOT operate until the lamp has been OFF for
about 30 minutes.

The lamp may break.
lamp.
2 After removing the tubing from

the clamp (C), remove the lamp 6
cover mounting screw (A) and
remove the lamp cover (B).

6 PDA Detector
4 Remove the two W lamp

mounting screws (B) using a
Phillips screwdriver, and remove
the W lamp and socket (C).
5 Remove the W lamp (A) from the

socket (B).
6 Use a piece of wiping paper to

pick up the new W lamp (A) so
as not to get any dirt on the glass
surface, and insert it into the
socket (B).
• Insert the W lamp

until its feet reach
the bottom of the
socket.
• If dirt is attached
to the lamp, use a
piece of lens paper
with ethanol to
wipe it away.
Performance may
be affected if it is
dirty.

7 Place the plate W (A) and check

that the lamp emission point (B)
is centered. If it is not aligned,
adjust by moving the lamp head
in the direction of the arrow
shown in the drawing.
8 Attach the two W lamp

mounting screws (B) using a
Phillips screwdriver, and attach
the W lamp and socket (C).

screw (A) and attach the lamp
cover (B), and then attach the
tubing to the clamp (C).

6 PDA Detector
the [W lamp replacement] screen.
5 Select [Next].
value is reset.
maintenance log.

7 Perform exposure time calibration.

Integrity Guide "3.2.6 PDA Detector
■Exposure Time / W Lamp Voltage
Setting"

7 Other Inspection/Maintenance
7.1 Cleaning the Leak Tray
Leak trays are located at the detector, pump, and autosampler (high-pressure valve, valve
mount).
When a leakage occurs, completely wipe away the liquid on the leak tray following the
procedure below.
If buffer solution was used as the mobile phase solution, crystals formed from discharged
buffer solution that has dried may cause clogging in the leak tray outlet. When a leak
tray is dirty from buffer solution crystals, wipe the leak tray using a cloth moistened with
water following the procedure below.
The following is an explanation using the pump as an example.
System Guide "5.1.2 Cleaning the Leak Sensor and Tray"
1 Wipe completely away the leaked

liquid around the leak sensor (A) or on
the leak tray with wiping paper.
• Do NOT bend or pull on the

leak sensor.
• When a leak tray is dirty from
buffer solution crystals, wipe
the leak tray using a cloth
moistened with water.

7.2 Replacement of the Air Filter

Air filters are attached to the instrument. Clogging in the air filter causes a decrease in
instrument performance as well as a malfunction. If discoloration of the air filter due to
clogging occurs, carry out replacement with a new one.
Turn the instrument power OFF before replacing the filter.
Necessary parts
No. Part Name Part Type Part No. Remarks

Recommended
Consumable
1 Side Air Filter S228-55674 replacement cycle
part
(Approx. 1 year)
Recommended
Consumable
2 Front Air Filter S228-55674-01 replacement cycle
part
(Approx. 1 year)
Recommended
replacement cycle
Consumable
3 Air Filter for Detector S228-55674-02 (Approx. 1 year)
part
Common to the
UV/PDA Detector
7.2.1 Side Air Filter
The parts of the air filter used for both the right and left sides are the same.
The following is an explanation using the air filter for the left side as an
example.

1 While holding the lock (a) of the filter

cover (A), remove the filter cover.
2 Remove the air filter (B) from the filter

cover (A).
3 Attach a new air filter (B) to the filter

cover (A).
4 Insert the claw (a) of the filter cover

(A) into the filter cover positioner (b)
and attach the filter cover.

maintenance log.

7.2.2 Air Filter for UV/PDA Detector
The parts of the air filter used for both the UV detector and the PDA detector are the
same.
The following is an explanation using the UV detector as an example.
2 Loosen the lamp cover mounting screw

(B) of the detector, and remove the
lamp cover (A).
3 Take the air filter (A) out by pinching

it with fingers.

4 Insert a new air filter (A).
5 Attach the lamp cover (A) by inserting

its left-side protrusions (2 locations)
into the slits on the detector, then
tighten the lamp cover mounting
screw (B).

maintenance log.
7

7.2.3 Air Filter for the Autosampler
Removal and reattachment of the front panel is required for the replacement
of the air filter on the front bottom of the autosampler.

2 Gradually slide the air filter (A) with

fingers and pull it out from the
aperture (a).
3 Insert the new air filter (A) from the

aperture center and gradually slide the
filter to position both ends with the
left and right ends of the aperture (a,
b).



8 Auxiliary Functions
This section will explain how to operate the auxiliary functions. These auxiliary functions
are used to perform operation tests for each hardware unit.
8.1 Displaying Auxiliary Function Items

This will explain how to display each auxiliary function item.
1 Press (Menu).
The [Menu] screen opens.
The [Maintenance] screen is displayed.
3 Press (Validation).
The [Validation] tab is displayed.

4 Press [Auxiliary Operation].

The [Auxiliary Operation] screen is
displayed.
5 Press the target item.

8.2 Z-Home
8.2 Z-Home
This section explains how to move the needle to the center of the instrument (Z-Home).
1 Press [Z-Home] on the [Auxiliary Operation] screen.

"8.1 Displaying Auxiliary Function Items" P.155
The [Z-Home] screen is displayed.
2 Press [Z-Home] or [Standby Position] to

move the needle position.
• Z-Home: The needle moves up to
the highest point and
moves to the center of the
instrument (Z-Home).
• Standby The needle moves to the
position: start position (standby
position).
3 Press [Close].
8.3 Pump Pressure Zero Adjustment

This section explains how to adjust the pump pressure to "0".
1 Press [Pump Pressure Zero Adjustment] on the [Auxiliary Operation] screen.

The [Pump Pressure Zero Adjustment] screen is displayed.

8
2 Press [Execute].
Adjust the pump pressure to "0".
Check that the pump pressure monitor value at the top of the screen becomes "0".
3 Press [Close].

8.4 High-Pressure Valve Operation Check

The high-pressure valve flow line switches.
1 Press [HPV Operation Check] on the [Auxiliary Operation] screen.

The [HPV Operation Check] screen is displayed.
2 Press [Start].
The high-pressure valve flow line switches.
3 Press [Close].

8.5 Autosampler LED Operation Check
8.5 Autosampler LED Operation Check

This section explains how to turn the LED of the autosampler ON and OFF.
1 Press [Autosampler LED Check] on the [Auxiliary Operation] screen.

The [Autosampler LED Check] screen is displayed.
2 Press [ON] or [OFF] to turn the LED of

the autosampler ON or OFF.
• ON: The autosampler LED turns ON.
• OFF: The autosampler LED turns OFF.
3 Press [Close].

8.6 Autosampler Sensor Test

This section explains how to check whether the sensor in the autosampler is operating
properly.
1 Press [Autosampler Sensor Check] on the [Auxiliary Operation] screen.

The [Autosampler Sensor Check] screen is displayed.
2 Press [Start] and check the sensor in

the autosampler.
The test results are displayed in [Result].
• OK: The sensor is operating properly.
• NG: The sensor is not operating
properly.
A sensor error has been generated
by a sensor that is not operating
properly.
3 Press [Close].

8.7 Injection Port Operation Check
8.7 Injection Port Operation Check

This section explains how to move the needle up and down. Check that the needle moves
down to the injection port properly.
1 Press [Injection Port Operation Check] on the [Auxiliary Operation] screen.

The [Injection Port Operation Check] screen is displayed.
2 Press [Start] and check that the needle

moves down to the injection port
properly.
3 Press [Close].

Index
A N
Air Filter 150 Needle 18
Automatic Rinsing Kit 103 Needle Seal 14
Autosampler LED Operation Check 159 Needle seal XR tool 14
Autosampler Sensor Test 160
Auxiliary Functions 155 O
Outlet Tubing 64
B
Box driver 76 P
buffer solution 78, 86 Periodic Inspection/Maintenance 1
Plunger 76
C Plunger Seal 70
cell window 111, 115 Plunger tool 44
Check Valve 86, 89, 91 Pump Heads 66
Pump Pressure Zero Adjustment 157
D
D2 Lamp 120, 140 R
Diaphragm 76 Rack Panel 57
Diaphragm jig 76 Removing/Attaching the Front Panel 12
Displaying the Replaced Part Items 7 Rinsing Port Cap 55
Drain Valve ASSY 101 rinsing port cover 55
Rinsing the Flow Line 62
F
Ferrule 1.2F 20 S
Flow Cell 104, 107, 110, 124, 127, 130 Sample Loop 32
Front Panel 58 sample racks 57
Seal tool 39
G Seal tool 70, 76
Gasket 110, 115, 130, 135 Suction Filter 99
H W
head holder 77 W Lamp (Optional Part) 144
High-Pressure Valve Operation Check 158 Wiper 59
Inspecting (Replacing) the High-Pressure Valve
Rotor and High-Pressure Valve Stator 24 Z
Z mount cover 19
I Z-Home 157
Injection Port Operation Check 161
L
lamp cover 121, 141
Leak Tray 149
lens 111, 115, 135
lenses 131
Line Filter 95, 97
M
Maintenance 7
Maintenance Log 10
Measuring Pump Plunger Seal 39

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228-91532

SHIMADZU HIGH PERFORMANCE LIQUID CHROMATOGRAPH

ã 2013 Shimadzu Corporation. All rights reserved.

Prominence-i LC-2030 LC-2030C LC-2030C 3D i

ii Prominence-i LC-2030 LC-2030C LC-2030C 3D

Indications Used in This Manual

Indicates a potentially hazardous situation which, if not

Indicates a potentially hazardous situation which, if not

The following symbols are used in this manual:

Indicates an action that must not be performed.

Indicates an action that must be performed.

Indicates information provided to improve product performance.

Indicates the location of related reference information.

Prominence-i LC-2030 LC-2030C LC-2030C 3D iii

Use this instrument ONLY for the intended purpose.

Safety regulations and standards

Use of open flame in the vicinity of this instrument

The room where the instrument is installed should

Provide sink washing equipment as close to the

iv Prominence-i LC-2030 LC-2030C LC-2030C 3D

Keep away from equipment generating strong magnetic fields.

When transporting the instrument, do not hold it by the front panel.

The lab table on which this instrument is installed

Keep at least 100 mm between the rear of the

Install the instrument in a location that satisfies the following conditions to

Prominence-i LC-2030 LC-2030C LC-2030C 3D v

To ensure safe operation, contact your Shimadzu representative if product installation,

Do NOT place heavy objects on the power cord. Do

Use only the specified cable.

Do NOT insert the adapter's grounding lead into

Do NOT connect the grounding lead to the gas

To unplug the instrument, pull the plug itself, NOT

Take measures to prevent the instrument from falling in the event of an

vi Prominence-i LC-2030 LC-2030C LC-2030C 3D

Ground the instrument.

Connect the instrument only to a power supply of indicated voltage in

When installing the instrument, be careful not to

When opening the doors, be careful not to pinch

Before plugging in the instrument, make sure that

Prominence-i LC-2030 LC-2030C LC-2030C 3D vii

Do NOT use flammable sprays (hair sprays,

Never use a cracked reservoir bottle.

Do NOT apply water to office equipment such as the PC as well as the

Take thorough measures to prevent buildup of

Always wear protective gloves and protective

viii Prominence-i LC-2030 LC-2030C LC-2030C 3D

n Inspection and Maintenance

Never remove the main cover.

Replacement parts must be of the specifications given in "Component Parts"

n Repair, Disassembly and Modification

Do NOT modify or disassemble the product without permission.

When repair is necessary, request through your Shimadzu representative.

Prominence-i LC-2030 LC-2030C LC-2030C 3D ix

Emergency Shutdown Procedure

1 Turn the power to the instrument OFF.

2 Disconnect the power cable at the rear of the instrument.

n During a Power Outage

Take the following measures in the event of a power outage.

1 Turn the power to the instrument OFF.

x Prominence-i LC-2030 LC-2030C LC-2030C 3D

Static Electricity Precautions

n Typical Cause of Static Electricity Accidents

Static electricity accidents are generally caused by this sequence of events: