Assignment Topics

BTPC-304
Bioprocess Modeling and Simulation
B.Tech. 3rd Year (6th Sem.)

1. Development of a mathematical model for optimizing bioprocess parameters.

2. Comparison of different simulation techniques for predicting the performance of
bioprocesses.
3. Simulation of bioprocessing techniques for protein purification.
4. Investigation of the effect of feed rate and temperature on the performance of a
continuous fermentation process using process simulation.
5. Simulation of microbial growth and product formation in a bioreactor under different
operating conditions.
6. Optimization of bioprocess parameters for the production of biofuels using a process
simulation approach.
7. Comparison of different bioreactor designs using simulation techniques for enhanced
production of pharmaceuticals.
8. Evaluation of the feasibility of a bioprocess design using simulation software and
sensitivity analysis.
9. Modelling the production of antibiotics using bioprocess techniques
10. Modelling and simulation of fed-batch fermentation for enhanced bioprocess
efficiency
11. Quantitative analysis of metabolic pathways in bioprocesses using modelling and
simulation
12. Modelling and simulation of enzymatic reactions in bioprocess techniques
13. Simulation of a continuous stirred-tank bioreactor (CSTB) for the production of a
specific bioproduct.
14. Modeling and simulation of a packed-bed bioreactor for the synthesis of a high-value
bioproduct.
15. Evaluation of the performance of a fluidized-bed bioreactor for the production of a
specific bioproduct.
16. Comparison of the performance of batch and fed-batch bioreactor systems for
bioproduct synthesis.
17. Optimization of the feeding strategy in a fed-batch bioreactor for the production of a
specific bioproduct.
18. Analysis of the scale-up of a bioreactor system for the synthesis of a bioproduct.
 Instructions for Assignment Submission

Overall, the structure of an assignment should be organized in a clear and concise manner
typically consisting of the following sections that effectively communicates the main ideas and
findings.

1. Define the bioproduct: The first step in simulating a bioprocess is to define the
bioproduct you want to produce. This will determine the type of microorganism you
will need to use, the growth conditions that will be required, and the yield of the
product.
2. Define the bioreactor parameters: Next, you will need to define the parameters of the
bioreactor itself. This includes the size of the reactor, the agitation rate, the
temperature, and the pH.
3. Select the microorganism: Based on the bioproduct you want to produce and the
growth conditions required, you will need to select a suitable microorganism to use in
the bioreactor.
4. Set up the mass balance equations: The mass balance equations describe the flow of
materials in and out of the bioreactor. You will need to set up equations that describe
the flow of nutrients, microorganisms, and products.
5. Solve the equations: Using a numerical solver, you can solve the mass balance
equations to predict the behavior of the bioreactor over time. This will allow you to
predict the concentration of microorganisms and products, as well as the yield and
productivity of the bioprocess.
6. Optimize the process: Once you have a working model of the bioprocess, you can use
it to optimize the process parameters. This may involve adjusting the agitation rate,
temperature, pH, or other parameters to maximize the yield or productivity of the
bioprocess.
7. Data required for simulation: You can refer any experimental paper to get the data for
the simulation. Else, you can generate the random data for the simulation as per the
following example:

Substrate Biomass Product

Time Concentration Concentration Concentration
(hours) (g/L) (g/L) (g/L)
0 50 0 0
10 40 2.5 0.5
20 35 4.7 1.2
30 30 7.9 2.5
40 25 11.3 4.2
50 20 14.8 6.5
60 15 18.5 9.2
 Substrate Biomass Product
Time Concentration Concentration Concentration
(hours) (g/L) (g/L) (g/L)
70 10 22.2 12.5
80 5 26.1 16.2
90 0 30.0 20.5

8. There are several freely available and easy-to-use simulation tools for bioprocess
engineering that you can use. Here are a few suggestions:

1. BioSIMI: BioSIMI is a web-based simulator for bioprocess engineering. It allows you

to simulate a variety of bioprocesses, including fermentation, enzyme-catalyzed
reactions, and more. It is free to use and requires no installation.
2. Virtual Bioprocessor: Virtual Bioprocessor is a bioprocess simulator that allows you
to simulate a variety of bioprocesses, including fed-batch and continuous bioreactor
operations. It is available for free download and use.
3. Aspen Plus: Aspen Plus is a process simulation tool that is widely used in the
chemical and bioprocess industries. While it is not free, it does offer a free trial
period, and it is known for its user-friendly interface and ease of use.
4. BioPAT® SIMCA®: BioPAT® SIMCA® is a bioprocess modeling and analysis tool
that is specifically designed for bioprocess engineering. While it is not free, it does
offer a free trial period, and it is known for its ability to handle large data sets and
provide in-depth analyses.
5. SuperPro Designer: SuperPro Designer is a comprehensive process simulator that is
widely used in the bioprocess industry. While it is not free, it does offer a free trial
period, and it is known for its user-friendly interface and ability to simulate a wide
range of bioprocesses.
6. EasyBioProcess: EasyBioProcess is a free, web-based bioprocess simulator that
allows you to model and simulate bioprocesses, including batch and fed-batch
fermentation, and downstream processing. It also includes a library of pre-built
bioprocess models that you can use as a starting point for your own simulations.
7. Bioreactor Simulator 3: Bioreactor Simulator 3 is a free, open-source software tool for
simulating bioprocesses in bioreactors. It allows you to model various bioprocesses,
including fed-batch, continuous, and perfusion bioreactors, and includes a range of
features for data analysis and visualization.
8. BioProSA: BioProSA is a free, web-based software tool for simulating bioprocesses
in both batch and fed-batch bioreactors. It includes a user-friendly interface and a
range of features for data analysis and visualization.
9. BioDyn: BioDyn is a free, open-source software tool for simulating bioprocesses in
both batch and continuous bioreactors. It includes a range of features for data analysis
and visualization, and can be customized to fit the specific needs of your bioprocess.
10. BioPAT® MFCS: BioPAT® MFCS is a bioprocess control software tool that includes
a range of features for modeling, simulation, and control of bioprocesses. It is not
free, but it does offer a free trial period and is known for its user-friendly interface
and ability to integrate with a range of bioreactor systems.
N.B.: 1. There is flexibility in choosing the topics of assignment.
2. The assignment could be done individually or in groups (not more than 3 members in
a group), and not more than 3 individuals/groups have the same topic.
3. Similarity index should be less than 20%
4. Submission dead line 25/04/2023