First the, plasmid is isolated from the bacteria cell.

Secondly,Restriction
enzyme is used to isolate the Gene from human body. The same restriction enzyme is used to cut
the plasmid and leaving it with sticky ends. If both plasmid and gene is complementory in shape,
ligase will stick them together. Then the recombinant
DNA is inserted into a new bacteria cell. The bacteria is put into the
fermenter with nutrient broth which contain carbohydrates and amino acid which is used for their
grown, respiration, reproduction and proteinsynthesis. Substrate of bacterial enzyme is also
added as a culture medium. There is also a pH and temperature probe which control the pH and
temperature to optimum. Oxygen is suppplied from sparger which is used for aerobic respiration.
Cooling jacket, outside the fermenter keep the fermenter cool if it become hot. A pump is also
connected inside the fermenter which carries out the waste product for eg. CO2. Under these
conditon the bacteria is placed 4-5 days and the bacteria produce product when the nutrients
finishes. At last, the product is collected from the harvester and then purified and crytallized. And
finally the product is obtained.