CC3 Lab Exp

Name: _________________________________________________ Date: __________________
Section: __________________ Group: __________________ Score: _________________
EXPERIMENT 3 DETERMINATION OF ALCOHOL
1. Explain the different biochemical substances, their metabolism, actions, reference

ranges and variables that may affect their analyses.
COURSE
2. Correlate test results with pathologic conditions. OUTCOMES
3. Apply concepts and principles of instrumentation in the laboratory
To achieve this unit, the learner must:

OUTCOMES
1. Be able to determine the alcohol concentration using the rapid saliva test.
2. Be able to perform the saliva alcohol test correctly
UNIT
3. Be able to accurately interpret the result of the assay based on

the color change on the reaction pad.
TEACHING AND LEARNING ACTIVITIES
Laboratory Experimentation
Alcohol is consumed by two-thirds of adults. It is metabolized by alcohol dehydrogenase in the liver

and produces formic acid as its by-product. It affects the central nervous system and is among the leading
causes of intoxication-related road accidents.
The concentration of alcohol in saliva is comparable with concentration of alcohol in blood. The
Saliva Alcohol Test Strip is a rapid test which utilizes an enzymatic system to detect 0.02% or greater
concentrations of blood alcohol levels in saliva specimens.
PRE-ANALYTICAL PHASE
Upon the receipt of laboratory specimen in the laboratory, the medical technology should check the
label (name, age, sex, time of collection and date of collection) attached to the specimen container together
with the requisition slip. He should verify the proper patient preparation, collection procedure and
transportation of specimen to the patient. And patient sample received should be noted to appropriate
logbooks in the laboratory for documentation purposes.
11 | C L I N I C A L C H E M I S T R Y 3 L A B O R A T O R Y M A N U A L
OUTCOMES 1. Be able to determine the alcohol concentration using the rapid saliva test.
UNIT

ANALYTICAL PHASE
Materials
1. Test strips 5. Pipette

2. Collection cups 6. 80 proof distilled spirits
3. Graduated cylinder 7. Distilled water
4. Timer
ANALYTICAL PHASE
Procedure
A. Quality control
1. Bring the pouch to room temperature of 15-7˚C prior to testing.
2. Remove the test trip from the sealed pouch and use it as soon as possible.
3. Observe the color of the reaction pad located on the test strip. It should have a light cream. (If it
has a blue color or discolored before saliva specimen is applied, do not use the test strip.)
4. Saturate the reaction pad with the prepared test solution for 6-8 seconds.
5. Start the 2-minutes timer after saturating the reaction pad with the test solution.
6. Read results at 2 minutes by visually comparing the color of the reaction pad to the corresponding
color blocks printed on the pouch. (Do not interpret the result after 3 minutes.) The color change
on the reaction pad should correspond to blood alcohol concentration of 0.02% or greater as
compared to the color chart printed on the pouch.
B. Detection of alcohol in saliva

1. Bring the pouch to room temperature of 15-7˚C prior to testing.
2. Remove the test strip from the sealed pouch and use it as soon as possible.
3. Observe the color of the reaction pad located on the test strip. It should have a light cream color.
(If it has blue color or discolored before saliva specimen is applied, do not use the test strip.)
4. Saturate the reaction pad with saliva from the collection cup for 6-8 seconds.
5. Start the 2-minutes timer after saturating the reaction pad with saliva.
6. Read results at 2 minutes by visually comparing the color of the reaction pad to the corresponding
color blocks printed on the pouch. (Do not interpret the result after 3 minutes.)
C L I N I C A L C H E M I S T R Y 3 L A B O R A T O R Y M A N U A L |12
OUTCOMES 1. Be able to determine the alcohol concentration using the rapid saliva test.
UNIT

7. Illustrate the results and write its corresponding interpretation on the activity sheet.
POST-ANALYTICAL PHASE
Cleaning and Disinfection
The specimen should be disinfected with 10% bleach or 10% Lysol before disposal in infective
wastes bags. All materials used should be disposed. The working area should be cleansed with the
disinfectant before leaving.
The PPE of each individual should be removed properly. These cannot be exposed outside the
laboratory premises.
Critical Thinking
1. The Saliva alcohol test strip only provides a preliminary result for the relative Blood Alcohol
concentration (BAC). What should be performed to provide a confirmatory result for BAC?
2. Interpreting the results for rapid tests such as the saliva alcohol test strip has subjective variability.
Enumerate the factors which influence the interpretation of results. What measures should be taken to
avoid misinterpretation of results?
3. Give 10 examples of substances which could interfere with the Saliva Alcohol Test Strip.
4. This assay is specific for which alcohols.
OUTCOMES
1. Be able to determine the alcohol concentration using the rapid saliva test.
UNIT


1. Be able toEXERCISE
determine the # 3- DETERMINATION
alcohol concentration usingOF theALCOHOL
rapid saliva test.
NAME______________________________
the color change on the reaction pad. SECTION____________
DATE__________
PATIENT’S NAME:_____________ SEX:_____________
AGE:__________
1. Color change on reaction pad of Saliva Alcohol Test Strip:
2. Interpretation of Result:
_______________________
NEGATIVE: No color change appears on the reaction pad. The color should match
the color block on the pouch corresponding to a negative (-) result. This indicates that
alcohol has not been detected.
POSITIVE: A color change appears on the reaction pad. The BAC will range from
0.02% to 0.30%, with the color on the reaction pad varying from a light blue to dark blue,
falling on or between the corresponding color blocks on the pouch.
INVALID: The outer edges of the reaction pad produces a slight color but the
majority of the reaction pad remains colorless. Repeat the test using a new test strip,
ensuring complete saturation of the reaction pad with saliva.
Name: _________________________________________________ Date: __________________
EXPERIMENT 4 DRUG TEST SPECIMEN COLLECTION
1. Explain the different biochemical substances, their metabolism, actions, reference ranges and
variables that may affect their analyses. COURSE
3. Perform correctly laboratory assays used to measure concentration of specific analytes

OUTCOME
1. To efficiently collect acceptable specimens for drug testing in the screening

UNIT
laboratory.
As mandated by Republic Act 9165, specimens which, will be tested for the presence of substances of
abuse should be collected following the standard set forth by the National Reference Laboratory under the
Department of Health.
ANALYTICAL PHASE
Materials
a. Zip-lock plastic containers

b. Wide mouth plastic urine containers
c. Analytical balance
OUTCOME
UNIT

laboratory.
Procedure
1. Urine
a. Collect 60ml into a clean, dry wide-mouth plastic container.
b. Label as follows:
Name:
Age:
Sex:
Volume:
Date Collected:
Time Collected:
Signature of authorized specimen collector:
Signature of client:
Storage: Prolonged storage -20˚C
2. Scalp hair
a. Weigh 100 mg hair or its equivalent in number of strands centimers above the scalp
Name:
Age:
Sex:
Weight:
Date Collected:
Time Collected:
Storage: Stored at cool, dry place
OUTCOME
UNIT

laboratory.
3.Saliva (Oral fluid)

a. Collect 2 ml in a dry, clean plastic container avoiding inclusion of food debris.
Name:
Age:
Sex:
Volume:
Date Collected:
Time Collected:
Storage: Frozen at -8 to -10 ˚C
OUTCOMES
UNIT

laboratory.
Critical Thinking
1. Aside from urine, saliva and scalp hair, what are the other specimens which could be collected for
drug testing?
2. If the specimen provided by the client is insufficient, what should be the corrective measure
performed be the laboratory personnel?
3. If a fluid received by the analyst in the laboratory is labeled as urine but the analyst suspects
otherwise, what test could be performed to confirm the validity of the specimen?
OUTCOMES
UNIT

laboratory.
EXERCISE # 4- DRUG TESTING SPECIMEN COLLECTION
NAME_________________________________ SECTION_________
DATE________________________
Tabulate the storage and volume of specimens necessary to conduct drug tests.
SPECIMEN AMOUNT STORAGE
(VOLUME)
1.
2.
3.
4.
5.
Illustrate a properly labeled urine specimen container.
Name:____________________ Age: _____ Sex:_____
Volume:__________________
Date Collected:________________
Time Collected:__________________
Signature of authorized specimen collector:_____________
Signature of client:_____________
Storage: Prolonged storage -20˚C

OUTCOME
UNIT

laboratory.
ASSESSMENT CRITERIA FOR PASSING
Outcome To achieve each outcome, the learner must

demonstrate the ability to:
1. Explain the significance in performing drug a. Explain the procedure and the importance of drug
testing. test collection to the patient.
2. Identify various drugs that can affect the body b. Followed the drug test collection standards set by.
functions. c. Perform the collection accurately.
d. Interpret the drug test result accurately.
3. Perform the collection procedure under Chain of
Custody (COC)
4. Correlate& Interpret results of drug test with
accuracy.
ASSESSMENT STRATEGIES
Focus for Assessment
ANECDOTAL NOTES
Observe students as they participate in the activity. Note the extent to which students are able to make and talk about
personal observation. Record anecdotal notes.
OBSERVATION CHECKLIST
Create an outcome-based checklist and share with students prior to beginning the activity. Use the checklist to
assess if students are able to make and talk about personal observations.
RUBRIC
Collaboratively create an outcome – based rubric with students. Use the rubric to evaluate how well the students are
able to understand cells.
RESOURCES
 Adapted from the DOH- National Reference Laboratory Manual of Operations for Screening
Drug Testing Laboratories
Name: _________________________________________________ Date: __________________
EXPERIMENT 5 DRUG TESTING ACCESSIONING

1. Explain the different biochemical substances, their metabolism, actions, reference ranges
COURSE
and variables that may affect their analyses. OUTCOMES
2. Correlate test results with pathologic conditions.
OUTCOMES
1. Explain the significance of drug accessioning.

UNIT
2. To conduct accessioning for a drug test in a screening laboratory.
In order to efficiently conduct the drug test, accessioning is a crucial step. It is the manner in
which documents are controlled for each specimen received and processed in the laboratory.
ANALYTICAL PHASE
Materials
a. Logbook d. Document forms

b.Marker
c.Gum Lables
OUTCOMES 1. Explain the significance of drug accessioning.
UNIT
NAME_______________________________________ SECTION____________
DATE________________________
Accessioning
1. Drug Testing Consent Form

Name of the Laboratory
Address
Tel.No.
DRUG TESTING CONSENT FORM

(Form DT-001)
Code No:__________
Name:__________________ Date_________ Time_________
Address:_________________ Contact No:__________
Birthdate:_________________ Age:________________
Sex://Male //Female Civil Status:__________
Company:________________________________________
Purpose of drug test:
//Pre-employment //Random //Reasonable Suspicion/Case
//Return to Duty //Mandatory //Post Accident
//License //Follow up //Others (please specify)____________
Instruction: Answer the questions below by checking the appropriate spaces. Afterwards,
read the statements below by signing.
Have you taken medication or drugs in the past 30 days? //Yes //No
Have you ingested any alcoholic beverage in the past 24 hours? //Yes //No
If you are taking medications or drugs list them below:
__________________________________________________________________
I hereby consent and agree to give sample of my: (Please. Encircle)

a.)Urine b.) Blood c.) Saliva d.) Hair e.) Sweat f.)Tissues
The result of any tests performed shall be provided to the requesting office or agency. My
signature below acknowledges that I have read and understood the foregoing statement
and I have answered all the questions truthfully.
Date:________________ Signature:______________________
I hereby consent and agree that my _________specimen, if found positive be sent to duly
accredited/licensed Confirmatory Laboratory for confirmatory test.
I hereby acknowledge that the ______ sample is my own and that the samples were
sealed in my presence. These samples are to be tested for dangerous drugs.
Date:_________________ Signature:_____________________
2. Custody and Control Form
Name of Laboratory__________________ Address________________________ Tel.No._____________
CUSTODY AND CONTROL FORM (Form DT-002A-COPY FOR THE DONOR)
SPECIMEN ID NO.____________ LAB ACCESSION NO.________

STEP1 COMPLETED BY COLLECTOR OR EMPLOYER REPRESENTATIVE
_________________________________________________________________________________________________
Client’s/Donor’s/Subject’s Name:__________________________ B. Address_____________________________ C.Age_____D.Sex_____
Employer Name and Address:_________________________________________________________
Type of Specimen: G.Reason for Test:
//Urine //Pre employment //Random //Reasonable Suspicion/Cause
//Blood //Return to Duty //Mandatory //Post Accident
//Others (specify) //Follow up //Others (specify)_________
Drug tests to be performed: //THC,COC,PCP,OPI,AMP //THC & MET //Others (specify)___________________

STEP2 COMPLETED BY COLLECTOR
________________________________________________________________________________________________________________
A specimen temperature within 4 minutes. Specimen Collection: //Observed //Unobserved Other Observation
Temperature between 32C and 38C? Specimen Sampling: //Single //Split (Enter Remark)
//Yes //No Specimen Volume:________ml Physical appearance:
Color:____________
REMARK:_______________________________________________________________________
Collector affixes bottle seal(s) to bottle(s). Collector dates seal(s). Donor initial seal(s). Donor complete STEP 5.
CHAIN OF CUSTODY—INITIATED BY COLLECTOR AND COMPLETED BY LABORATORY
_________________________________________________________________________________________________________________
I certify that the specimen given to me by the donor identified in the certification section on Step 5 of this form was collected, sealed and
released to the Delivery Service noted in accordance with applicable Department of Health requirements.
______________________________ ___________________AM/PM SPECIMEN BOTTLE (S) RELEASED TO:
Signature of Collector Time of Collection
______________________________ ___/______/_____ _________________________________
(PRINT) Collector’s Name (First,MI,Last) Date(Mo/Day/Yr) Name of Delivery Service Transferring Specimen to Lab
_________________________________________________________________________________________________________________
RECEIVED AT LAB:
STATUS OF THE SPECIMEN SPECIMEN BOTTLE (S) RELEASED TO:
(a) Seal intact //Yes //No
______________________________ (b) Transport Device ___________ ___________________________________
Signature of Accesioner (c) Description_______________ Signature & Printed Name of Receiving Person
_______________________________ ____/_____/______ ______________________ ____/_____/_____

Accesioner’s Name (First, MI.Last) Date (Mo/Day/Yr) (PRINT) Name (First/MI.Last) Date (Mo/Day/Yr)
STEP 5 COMPLETED BY DONOR
I certify that I provided my urine specimen to the collector, that I have not adulterated it any manner, each specimen bottle used was sealed
with tamper-evident seal in my presence;
________________________________________ ________________________________ ____/____/______
Signature of Donor (PRINT) Donor’s Name (First,MI,Last) Date(Mo/Day/Yr)
Contact No. __________________________ Date of Birth:____/_____/_____
Additional information may be asked from you by the laboratrory particular on drugs and medications.
STEP 6 COMPLETED BY HEAD OF SCREENING LABORATORY
_________________________________________________________________________________________________________________
In accordance with applicable Department of Health requirements, my determination/ verification is:
// NEGATIVE //POSITIVE //TEST CANCELLED //REFUSAL TO TEST BECAUSE:

//DILUTED //ADULTERATED //SUBSTITUTED
REMARKS___________________________________________________ OTHER SPECIFY_________________________

X____________________________________________ __________________________________ ______________________
Name of Analyst (First,MI,Last) Signature & Name of Laboratory Date (Mo/Day/Yr)
3. Drug Test Report
Name of Laboratory
Address
Tel.No.
DRUG TEST REPORT

(FORM DT-003)
Accession No.:_________ Date and Time Received:____________
Name:_____________________ Date and Time Reported:____________
Last Name, First Name, Middle Name
Age:_________ Sex:___________
Requesting Party:________________________________________________
Purpose:
___Pre-employment ____Random ____Reasonable Suspicious/Cause
___Follow-up ____Mandatory ____Post-Accident
___Return-to-Duty ____Others (specify)________________________
Results:
Test Method:
____ Test Kit _____Instrumented (pls. Specify)
____ GC-MS for Confirmatory
Drug/Metabolite Result
Methamphetamine (MET)
Tetrahydrocannabinol (THC)
Other (specify)
Remark(s):___________________________________________________________
Reviewed by: (if applicable)
___________________ ______________________
Analyst Supervisor
Approved by:
____________________________
Head of the Laboratory
5. Receiving/Accessioning/Releasing Logbook
Date &Time Access Code Donor’s Age Sex Requesting Released Received Remarks
Collected ion no. no. name Party by by
OUTCOMES 1. Explain the significance of drug accessioning.
UNIT
Procedure
1. Answer the following forms to be utilized in drug testing if you were the client:
a. Drug testing consent form
b. Custody and control form (Copy for the screening laboratory)
c. Custody and control form (Copy for the donor)
d. Custody and control form (Copy for the collection site)
e. Custody and control form (Copy for the confirmatory)
2. Answer the following forms to be utilized in drug testing if you were the analyst:
a. Custody and control form (Copy for the screening laboratory)
b. Custody and control form (Copy for the donor)
c. Custody and control form (Copy for the collection site)
d. Custody and control form (Copy for the confirmatory
e. Drug test result
3. Answer the following forms to be utilized in drug testing if you were the accessioner:
a. Custody and control form (Copy for the screening laboratory)
b. Custody and control form (Copy for the donor)
c. Custody and control form (Copy for the collection site)
d. Custody and control form (Copy for the confirmatory)
4. Accomplish the Receiving/Releasing logbook.
Critical Thinking
1. Explain why is there is a need to accession specimens received in the laboratory.
2. What are the qualifications for an accessioner?
3. Enumerate five disadvantages of erroneous accessioning of specimens.
Name: _________________________________________________ Date: __________________
EXPERIMENT 6 DETERMINATION OF BARBITURATES
To achieve this unit a learner must:

OUTCOMES
1. Be able to determine the presence of Barbiturates in the urine specimen using the
rapid, one step Barbiturates test device.
UNIT
2. Be able to perform the immunoassay correctly.

3. Be able to accurately interpret the result of the assay based on the appearance of
colored line on the test region.
Barbiturates are sedatives, hypnotics, anti-convulsants, which are also central nervous system
depressants. Chronic use of Barbiturates leads to tolerance to physical dependence. Barbiturates taken at 400
mg/day for 2-3 months produce significant dependence. The withdrawal symptoms of drug abstinence can
be severe enough to cause death. Barbiturates are unaltered in the urine and could be detected for 4-7 days.
The Barbiturates test device utilizes a monoclonal antibody to selectively detect elevated levels
of Barbiturates in urine.
Upon the receipt of laboratory specimen in the laboratory, the medical technology should
check the label (name, age, sex, time of collection and date of collection) attached to the specimen container
together with the requisition slip. He should verify the proper patient preparation, collection procedure and
OUTCOMES
1. Be able to determine the presence of Barbiturates in the urine specimen using the rapid, one step
Barbiturates test device.
UNIT

3. Be able to accurately interpret the result of the assay based on the appearance of colored line on the test
region.
ANALYTICAL PHASE
Materials
1. Test devices 3. Timer

2. Specimen collection container 4. Droppers
Procedure
A. Quality Control
An internal procedural control line will appear in the control line region (C). It confirms
sufficient specimen volume, adequate membrane wicking and correct procedural technique.
B. Detection of Barbiturates in urine

1. Bring the pouch and the urine to room temperature of 15-30˚C prior to testing.
3. Place the test device on a clean and level surface.
4. Hold the dropper vertically and transfer 3 full drops of urine (approximately 100µL) to the
specimen well (S) of the test device. (Avoid trapping air bubbles in the specimen well (S).)
5. Start the 5-minutes timer after dropping the urine on the specimen well (S).
6. Wait for the colored line/s to appear.
7. Read results at 5 minutes by visually comparing the appearance of colored line/s on the
respective regions printed on the pouch. (Do not interpret the result after 10 minutes.)
OUTCOMES
UNIT

region.
Critical Thinking
1. A negative result in the test device for Barbiturates may not necessarily indicate Barbiturates-free
urine. Explain why.
2. If adulteration is suspected, the test should be repeated using a new urine specimen. What are
examples of adulterants which would interfere with test results for Barbiturates detection in urine?
3. Two subsequent urine test devices for Barbiturates were interpreted as invalid. Both test devices
came from same lot. What would be the next best course of action for the clinical laboratory
scientist?
OUTCOMES
UNIT

region.
EXERCISE # 6 – DETERMINATION OF BARBITURATES
NAME_____________________________________ SECTION___________
DATE______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________
Colored region/s on the test kit:

BAR
Interpretation of Result: ______________________
 NEGATIVE: Two lines appear. One colored line should be in the control region
(C), and another apparent colored line should be in the test region (T). The
negative result indicates that the Barbiturate concentration is below the detectable
cut-off level.* The shade of color in the test line region (T) may vary, but it should
be considered negative whenever there is even a faint colored line.
 POSITIVE: One colored line appears in the control line region (C). No line
appears in the test line region (T). This positive result indicates that the
Barbiturate concentration exceeds the detectable cut-off level.
 INVALID: Control line fails to appear. Insufficient specimen volume or incorrect
procedural techniques are the most likely reasons for control line failure. Review
the procedure and repeat the test with a new test device.
Name: _________________________________________________ Date: __________________
EXPERIMENT 7
DETERMINATION OF BENZODIAZEPINES

OUTCOMES
1. Be able to determine the presence of Benzodiazepines in the urine specimen using

the rapid, one step Benzodiazepines test device.
UNIT

Benzodiazepines are clinically used as sedative for surgical and medical procedures,
treatment for anxiety and sleep disorders. The risk of physical dependence increases if Benzodiazepines are
taken regularly for more than a few months at higher than normal doses. Symptoms such as trouble in
sleeping, gastrointestinal upset, feeling unwell, loss of appetite, sweating, trembling, weakness, anxiety, and
changes in perception will be felt upon stopping taking Benzodiazepines abruptly. Less than 1% is excreted
unaltered in the urine for 3-7 days.
The Benzodiazepines test device utilizes a monoclonal antibody to selectively detect elevated levels of
Benzodiazepines in urine.
OUTCOMES 1. Be able to determine the presence of Benzodiazepines in the urine specimen using the
rapid, one step Benzodiazepines test device.
UNIT

3. Be able to accurately interpret the result of the assay based on the appearance of colored
line on the test region.
ANALYTICAL PHASE
Materials

2. Specimen collection container 4. Droppers
Procedure
A. Quality Control
B. Detection of Benzodiaepines in urine

OUTCOMES
1. Be able to determine the presence of Benzodiazepines in the urine specimen using the rapid, one step
Benzodiazepines test device.
UNIT

region.
Critical Thinking
1. Enumerate reasons why erroneous results occur in the clinical laboratory, and what are possible
measures to prevent them.
2. The Benzodiazepines test device is a qualitative, preliminary test which would be able to detect
whether Benzodiazepines are present in the urine specimen at the cut-off concentration of 300
ng/mL. Is it possible to have a urine specimen which was interpreted as POSITIVE using the
Benzodiazepines test device to be interpreted as NEGATIVE by Gas Chromatography/Mass
Spectrometry? Justify your answer.
OUTCOMES
1. Be able to determine the presence of Benzodiazepines in the urine specimen using the rapid, one step
Benzodiazepines test device.
UNIT

region.
3. A study was conducted by the manufacturer of the benzodiazepines test device to determine
whether some compounds would have cross-reactivity at levels of 100µg/mL. List down examples
of non cross-reacting compounds
NAME_____________________________________ SECTION___________
DATE_______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________
BZO
 NEGATIVE: Two lines appear. One colored line should be in the control region (C), and
another apparent colored line should be in the test region (T). The negative result indicates
that the Benzodiazepines concentration is below the detectable cut-off level.* The shade of
color in the test line region (T) may vary, but it should be considered negative whenever
there is even a faint colored line.
 POSITIVE: One colored line appears in the control line region (C). No line appears in the
test line region (T). This positive result indicates that the Benzodiazepines concentration
exceeds the detectable cut-off level.
procedural techniques are the most likely reasons for control line failure. Review the
procedure and repeat the test with a new test device.
Name: _________________________________________________ Date: __________________
EXPERIMENT 8 DETERMINATION OF COCAINE

OUTCOMES
1. Be able to determine the presence of Cocaine in the urine specimen using the rapid,
one step Cocaine test device.
UNIT

Cocaine is self-administered through nasal inhalation, intravenous injection and free base smoking.
It has a biologic half –life of 0.5 to 1.5 hours. It is a potent central nervous system stimulant and a local
anesthetic. It brings about extreme energy and restlessness initially, then gradually resulting in tremors, over-
sensitivity and spasms. It causes fever, unresponsiveness, and difficulty breathing and unconsciousness in
large amounts.
The Cocaine test device utilizes a monoclonal antibody to selectively detect elevated levels of
Cocaine in urine. It yields a positive result when the Cocaine metabolite, Benzoylecgonine, which, could be
detected for 24-48 hours after exposure, with a biologic half-life of 5-8 hours exceed 300 ng/mL.
OUTCOMES 4. Be able to determine the presence of Cocaine in the urine specimen using the rapid,
UNIT

ANALYTICAL PHASE
Materials

2. Specimen collection container 4.Dropper
Procedure
A. Quality Control
B. Detection of Cocaine in urine

UNIT

Critical Thinking
1. The Cocaine test device only provides a preliminary qualitative analytical result. The preferred
confirmatory method for quantitating the concentration of Cocaine in the specimen is Gas
Chromatography/ mass Spectrometry. After performing the screening test for Cocaine, 3 specimens
were interpreted as POSITIVE and 10 specimens were interpreted as NEGATIVE. How many
specimens will be sent for Confirmatory testing? Why?
2. This symbol was seen on the box containing the test devices for Cocaine. What does it mean?
2
3. Another symbol was seen on the box containing the test devices for Cocaine. What does it mean?
UNIT

EXERCISE #8 – DETERMINATION OF COCAINE
NAME_____________________________________ SECTION___________
DATE_______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________
COC
Colored region/s on the test kit: C
negative result indicates that the Cocaine concentration is below the detectable cut-
off level.* The shade of color in the test line region (T) may vary, but it should be
considered negative whenever there is even a faint colored line.
appears in the test line region (T). This positive result indicates that the Cocaine
concentration exceeds the detectable cut-off level.
Name: _________________________________________________ Date: __________________
EXPERIMENT 9 DETERMINATION OF
METHYLENEDIOXYMETHAMPHETAMINE
(ECSTASY)

OUTCOMES
1. Be able to determine the presence of Methylenedioxymethamphetamine in the urine

specimen using the rapid, one step Methylenedioxymethamphetamine test device.
UNIT

Methylenedioxymethamphetamine (Ecstasy) is a designer drug first synthesized in 1914 by a

German drug company for the treatment of obesity. It is not a stimulant but similar to amphetamine drugs,
it has the capacity to increase blood pressure and heart rate. It also produces perceptual changes such as
increased sensitivity to light, difficulty in focusing, clenching of jaws and blurred vision in some users. Its
mechanism of action is due to release of the neurotransmitter, serotonin and dopamine.
The work area of the experiment should be sterilized. The medical technologist should wear proper
personal protective equipment before the experiment (laboratory gown, laboratory goggles or face-shield,
laboratory mask, and gloves). Universal precaution is applied to every activity of medical technologist to the
body fluid sample.
OUTCOMES
1. Be able to determine the presence of Methylenedioxymethamphetamine in the urine specimen using the
rapid, one step Methylenedioxymethamphetamine test device.
UNIT

region.
ANALYTICAL PHASE
Materials
1. Test devices
2. Specimen collection container
3. Timer
4. Droppers
Procedure
A. Quality Control
B. Detection of Methylenedioxymethamphetamine in urine

OUTCOMES
1. Be able to determine the presence of Methylenedioxymethamphetamine in the urine specimen using the
UNIT

region.
Critical Thinking Questions
1. The results for the Ecstasy test device should be read at 5 minutes. Cite the reasons why it should
not be read later than 10 minutes.
2. There are 3 compounds that are positively detected by the MDMA (Ecstasy) test device in urine at
specific concentrations. Complete the table below by listing down the specific concentration for
each compound which is detectable by the MDMA (Ecstasy) test device.
Compound Concentration (ng/Ml)
3,4-Methylenedioxymethamphetamine HCl (MDMA)
3,4-Methylenedioxyamphetamine HCl (MDA)
3,4-Methylenedioxyethylamphetamine (MDE)
3. What is the significance of knowing the lot number for the test devices utilized in the laboratory?
OUTCOMES 1. Be able to determine the presence of Methylenedioxymethamphetamine in the urine specimen using the
UNIT

region.
EXERCISE #8 – DETERMINATION OF METHYLENEDIOXYMETHAMPHETAMINE

(ECSTASY)
NAME_____________________________________ SECTION___________
DATE_______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________
MDMA
Name: _________________________________________________ Date: __________________
EXPERIMENT 10 DETERMINATION OF METHADONE

0
variables that may affect their analyses. OUTCOMES

1. Be able to determine the presence of Methadone in the urine specimen using the rapid,
one step Methadone test device.
AND LEARNING ACTIVITIES
ExperimentationLaboratory
Experimentation
Methadone is a narcotic pain reliever for medium to severe pain. It is also used in the treatment of
Heroin (Opiate dependence: Vicodin, Percocet, Morphine, etc.) addiction. Oral Methadone is very different
than the IV methadone. Oral methadone is partially stored in the liver for later use. IV Methadone acts
more like Heroin.
Methadone is a long-acting pain reliever producing effects that last between 12 to 48 hours. Ideally,
methadone frees the client from pressures of obtaining illegal Heroin, from the dangers of injection, and
from the emotional rollercoaster that most Opiates produce. The withdrawals from Methadone are more
prolonged and troublesome than those provoked by heroin cessation, yet the substitution and phased
removal of Methadone is an acceptable method of detoxification for patients and therapists.
OUTCOMES
1. Be able to determine the presence of Methadone in the urine specimen using the rapid, one step Methadone
test device.
UNIT

3. Be able to accurately interpret the result of the assay based on the appearance of colored line on the test region.
body fluid sample.
ANALYTICAL PHASE
Materials
1. Testdevices
3. Timer
4. Droppers
Procedure
A. Quality Control
B. Detection of Methadone in urine
OUTCOMES
test device.
UNIT

1. A positive result indicates presence of the drug or its metabolite in the urine specimen. Explain why
a positive result will not indicate the level of intoxication, administration route or concentration in
urine.
2. Identify what this symbols seen on the package insert of Methadone Test Device means.
30˚C
a. ___________________________
2˚C
b. ______________________________
!
OUTCOMES
test device.
UNIT

EXERCISE #8 – DETERMINATION OF METHADONE
NAME_____________________________________ SECTION___________
DATE_______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________
MTD
Name: _________________________________________________ Date: __________________
EXPERIMENT 11
DETERMINATION OF MORPHINE
1. Explain the different biochemical substances, their metabolism, actions, reference ranges and COURSE
variables that may affect their analyses. OUTCOMESCO
3. Perform correctly laboratory assays used to measure concentration of specific analytes URSE COURSE

OUTCOMES
1. Be able to determine the presence of Morphine in the urine specimen using the rapid,
one step Morphine test device.
UNIT

TEACHING AND LEARNING TEACHING
Laboratory
The Morphine Test Device for urine utilizes a monoclonal antibody to selectively detect elevated
levels of Morphine in urine and yields a positive result when the Morphine in urine exceeds 300 ng/mL.
Morphine is among the large group of Opioid analgesics which control pain by depressing the
central nervous system. It produces higher tolerance levels and physiological dependency in users, and may
lead to substance abuse. It is excreted unmetabolized in urine. Morphine is also the major metabolic product
of codeine and heroin. It is detectable in the urine for several days after an opiate dose.
body fluid sample.
1. Be able to determine the presence of Morphine in the urine specimen using the rapid, one step
OUTCOMES
Morphine test device.
UNIT

3. Be able to accurately interpret the result of the assay based on the appearance of colored line on the
test region.
ANALYTICAL PHASE
Materials
1. Testdevices
3. Timer
4. Droppers
Procedure
A. Quality Control
B. Detection of Morphine in urine

OUTCOMES 1. Be able to determine the presence of Morphine in the urine specimen using the rapid, one step
UNIT

test region.
1. Aside from the compound Morphine, the Morphine test device can also detect the presence of other
Morphine-related compounds in the urine specimen. Enumerate at least 5 examples of compounds
detected by the Morphine test device in urine.
2. Give examples of dietary intake which could cause a false-positive result in the Morphine test device
for urine specimen.
3. Complete the table below by writing down the concentration in nanograms per milliliter for each
compound detected by the Morphine test device.
Compound Concentration (ng/mL)
a. Norcodeine
b. Hydrocodone
c. Hydromorphone
d. Procaine
e. Thebaine
OUTCOMES
1. Be able to determine the presence of Morphine in the urine specimen using the rapid, one step
UNIT

test region.
EXERCISE #8 – DETERMINATION OF MORPHINE
NAME_____________________________________ SECTION___________
DATE_______________________
PATIENT’S NAME:______________________
AGE:_________
SEX:_________ MOP
C
 NEGATIVE: Two lines appear. One colored line should be in the control
region (C), and another apparent colored line should be in the test region (T).
The negative result indicates that the Cocaine concentration is below the
detectable cut-off level.* The shade of color in the test line region (T) may vary,
but it should be considered negative whenever there is even a faint colored line.
 INVALID: Control line fails to appear. Insufficient specimen volume or
incorrect procedural techniques are the most likely reasons for control line
failure. Review the procedure and repeat the test with a new test device.
Name: _________________________________________________ Date: __________________
EXPERIMENT 12 DETERMINATION OF METHAMPETAMINE AND

TETRAHYDROCANNABINOL

OUTCOMES
1. Be able to perform screening drug test for methamphetamine and tetrahydrocannabinol

UNIT
using immunoassay test kits.

2. Be able to accurately interpret the result of the immunoassay.
3. Be able to explain the principle of urine testing prohibited drugs.
A drug test is commonly a technical examination of urine, blood, semen, sweat, or oral fluid samples
to determine the presence or absence of specified drugs or their metabolized traces. It is a competitive-
binding immunoassay in which drug and metabolites in a urine sample compete with immobilized drug
conjugate for limited labeled antibody binding sites. By utilizing antibody that is specific to different drug
classes, the test permits independent, detection of drug from the sample.
body fluid sample.
OUTCOMES
UNIT
1. Be able to perform screening drug test for methamphetamine and tetrahydrocannabinol using
immunoassay test kits.
ANALYTICAL PHASE
Materials
1. Urine Specimen cups

2. Thermometer
3. Drug test kit for methamphetamine and tetrahydrocannabinol
4. Urinalysis test strip
Procedure
1. Allow the test panel and urine specimen to equilibrate to room temperature (15-30˚C) prior to
testing.
2. Bring the pouch to room temperature before opening it. Remove the test panel from the sealed
pouch and use it as soon as possible.
3. Take off the cap outside of the test end.
4. With arrow (s) pointing toward the urine specimen, immerse the test panel vertically in the urine
specimen for at least 10-15 seconds.
5. Do not pass the arrows on the test panel when immersing the panel. The same procedure is
followed for a multi-panel test card.
6. Place the test panel on a non-absorbent flat surface, start the timer and wait for the colored line (s)
to appear. The results should be read at 5 minutes. Do not interpret results after 10 minutes.
OUTCOMES
UNIT
1. Be able to perform screening drug test for methamphetamine and tetrahydrocannabinol using immunoassay test
kits.
1. The urine dipstick immunoassay only provides a presumptive result for the presence of drugs. What
is the confirmatory test for drugs?
2. What is the cut-off range for methamphetamine and tetrahydrocannabinol in the Philippines? Why was it
established at that level?
3. Enumerate 5 common adulterants for urine drug screening.
4. Give 3 reasons for rejection and cancellation of drug test.
5. If a faint line appears in the test region, what would be the official result of the test for that
particular drug?
OUTCOMES 1. Be able to perform screening drug test for methamphetamine and tetrahydrocannabinol using
UNIT

EXERCISE # 12- DETERMINATION OF METHAMPHETAMINE AND

TETRAHYDROCANNABINOL
NAME______________________________ SECTION_____________
DATE_________________
INTERPRETATION OF RESULTS
NEGATIVE: Two lines appear. One colored line should be in the control region (C), and another
apparent colored or faded color line adjacent should be in the test region (T). This negative result indicates
that the drug concentration is below the detectable level.
POSITIVE: One colored line appears in the control region (C). No line appears in the test region (T).
This positive result indicates that the drug concentration is above the detectable level.
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques
are the most likely reasons for control line failure. Review the procedure and repeat the test using a new
test panel. If the problem persists, discontinue using the lot immediately and contact your local distributor.
READING OF RESULTS
INTERPRETATION
TEST CONTROL SAMPLE
___________________________
________________________________
________________________________
OUTCOMES 1. Be able to perform screening drug test for methamphetamine and tetrahydrocannabinol using
UNIT

ASSESSMENT CRITERIA FOR PASSING
Outcome To achieve each outcome, the learner must

demonstrate the ability to:
1. Be able to perform screening drug test for methamphetamine 1. Determine specimens positive with
and tetrahydrocannabinol using immunoassay test kits. methamphetamine & THC.
2. Be able to accurately interpret the result of the immunoassay. 2. Perform the methamphetamine & THC
3. Be able to explain the principle of urine testing prohibited determination test correctly
drugs. 3. Interpret the result of the test kit accurately.
ASSESSMENT STRATEGIES
Focus for Assessment
ANECDOTAL NOTES
Observe students as they participate in the activity. Note the extent to which students are able to make and talk about
personal observation. Record anecdotal notes.
OBSERVATION CHECKLIST
Create an outcome-based checklist and share with students prior to beginning the activity. Use the checklist to
assess if students are able to make and talk about personal observations.
RUBRIC
Collaboratively create an outcome – based rubric with students. Use the rubric to evaluate how well the students are
able to understand cells.
RESOURCES
Adapted from the DOH-National Reference Laboratory Manual of Operations for Screening Drug Testing
Laboratories.

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Name: _________________________________________________ Date: __________________

Section: __________________ Group: __________________ Score: _________________

EXPERIMENT 3 DETERMINATION OF ALCOHOL

1. Explain the different biochemical substances, their metabolism, actions, reference

To achieve this unit, the learner must:

3. Be able to accurately interpret the result of the assay based on

TEACHING AND LEARNING ACTIVITIES

Alcohol is consumed by two-thirds of adults. It is metabolized by alcohol dehydrogenase in the liver

3. Be able to accurately interpret the result of the assay based on

1. Test strips 5. Pipette

B. Detection of alcohol in saliva

3. Be able to accurately interpret the result of the assay based on

Cleaning and Disinfection

4. This assay is specific for which alcohols.

2. Be able to perform the saliva alcohol test correctly

To achieve this unit, the learner must:

PATIENT’S NAME:_____________ SEX:_____________

1. Color change on reaction pad of Saliva Alcohol Test Strip:

EXPERIMENT 4 DRUG TEST SPECIMEN COLLECTION

To achieve this unit, the learner must:

1. To efficiently collect acceptable specimens for drug testing in the screening

TEACHING AND LEARNING ACTIVITIES

a. Zip-lock plastic containers

1. To efficiently collect acceptable specimens for drug testing in the screening

Signature of authorized specimen collector:

Storage: Prolonged storage -20˚C

Signature of authorized specimen collector:

Storage: Stored at cool, dry place

1. To efficiently collect acceptable specimens for drug testing in the screening

3.Saliva (Oral fluid)

Signature of authorized specimen collector:

Storage: Frozen at -8 to -10 ˚C

Cleaning and Disinfection

1. To efficiently collect acceptable specimens for drug testing in the screening

1. To efficiently collect acceptable specimens for drug testing in the screening

EXERCISE # 4- DRUG TESTING SPECIMEN COLLECTION

SPECIMEN AMOUNT STORAGE

Illustrate a properly labeled urine specimen container.

Name:____________________ Age: _____ Sex:_____

Signature of authorized specimen collector:_____________

Storage: Prolonged storage -20˚C

1. To efficiently collect acceptable specimens for drug testing in the screening

ASSESSMENT CRITERIA FOR PASSING

Outcome To achieve each outcome, the learner must

Focus for Assessment

EXPERIMENT 5 DRUG TESTING ACCESSIONING

To achieve this unit, the learner must:

1. Explain the significance of drug accessioning.

2. To conduct accessioning for a drug test in a screening laboratory.

TEACHING AND LEARNING ACTIVITIES

a. Logbook d. Document forms

2. To conduct accessioning for a drug test in a screening laboratory.

1. Drug Testing Consent Form

DRUG TESTING CONSENT FORM

I hereby consent and agree to give sample of my: (Please. Encircle)

SPECIMEN ID NO.____________ LAB ACCESSION NO.________

Drug tests to be performed: //THC,COC,PCP,OPI,AMP //THC & MET //Others (specify)___________________

_______________________________ ____/_____/______ ______________________ ____/_____/_____

// NEGATIVE //POSITIVE //TEST CANCELLED //REFUSAL TO TEST BECAUSE:

REMARKS___________________________________________________ OTHER SPECIFY_________________________

DRUG TEST REPORT

2. To conduct accessioning for a drug test in a screening laboratory.

1. Explain why is there is a need to accession specimens received in the laboratory.

Name: _______________________________ Date:

Section: Group: Score: _

PATIENT’S NAME:_ SEX:_

Name:__________________ Age: _ Sex:___

SPECIMEN ID NO.____ LAB ACCESSION NO.

_____________________________ /_/ ________________ /_/___

REMARKS___________________________ OTHER SPECIFY_