UNIVERSITY OF THE FREE STATE Question 2 [5]

You have available 1 M solutions of H3PO4 (pKa 2.15), H2PO4- (pKa 6,82) and HPO42- (pKa
MAIN CAMPUS 12,38). Explain how you will prepare 500 ml of a 100 mM phosphate buffer at pH 7,0. Show all
calculations.   
BOCB2616
Question 3 [5]
DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY Draw the detailed structure of the peptide Gly-Phe-Asp-Gln (at pH 7,0). Show all atoms and
charges.
Semester Test 1: 7 April 2022
Question 4 [5]
ASSESSOR: Prof. M.S. Smit Briefly explain what is meant by the “primary”, “secondary”, “tertiary” and “quaternary” structure of
MODERATOR: Prof. H.G. O’Neill proteins.

TIME: 1 hour MARKS: 50 Question 5 [5]

The figure below shows three-dimensional representations of four different proteins. Different
coloured (shades of grey) cartoons indicate different polypeptide chains. Which of these (A, B, C
Supplementary information or D) is most likely a representation of the structure of myoglobin? Explain your answer. Why
could the others not be myoglobin?

PART A [25 marks]

Online test to be completed in 30 min.

PART B [25 marks]
ANSWER ALL QUESTIONS ON TEST PAPERS. REMEMBER TO ENTER YOUR STUDENT NUMBER!

Question 1 [5]
A. Explain why the melting point of methane (CH4) (-182,5 ⁰C) is so much lower than that of ice
(H2O) (0 ⁰C), while their molecular weights (16 and 18) are very similar. (3)  
B. Explain what happens when fatty acids are mixed with water. (2)