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UNIVERSITI TUNKU ABDUL RAHMAN

CENTRE FOR FOUNDATION STUDIES

FHSC1214 FUNDAMENTALS OF CELL BIOLOGY

TUTORIAL 4
ENZYME I

[Source: Final examination, May 2023]

Q1. (a) Differentiate exergonic reaction and endergonic reaction. (6 marks)

(b) Figure 1 shows the movement of a vesicle along a microtubule track, guided by motor protein.

Figure 1

Answer the following questions based on Figure 1.

(i) Explain the role of ATP in powering the above mechanical work. (4 marks)

(ii) Name the other TWO (2) types of cellular work other than mechanical work.
(2 marks)

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[Source: Final examination, Sept 2022]
Q2. Figure 2 shows reaction X in human cellular respiration.

Figure 2

Answer the following questions based on Figure 2.

(a) Complete Table 2. (8 marks)

Table 2
Reaction Endergonic Exergonic
(Release/Absorb) free energy
Reactant
Product
Energy of reactants is
(lower/higher) than products

(b) Explain the roles of phosphoglycerate kinase in reaction X. (3 marks)

(c) Paul accidentally purified the phosphoglycerate kinase from the red blood cells with a pH 3
solution instead of a pH 7.5. Briefly explain how the pH changes affect the function of the
phosphoglycerate kinase. (5 marks)

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[Source: Final examination, January 2023]
Q3. (a) Complete Table 3.1 to show the contrast between ATP and deoxyribonucleotide. (6 marks)

Table 3.1
Criteria ATP Deoxyribonucleotide
Name of pentose
Name of nitrogenous base(s)
Number of phosphate group

(b) Complete Table 3.2 to show the contrast between photosynthesis and cellular respiration.
(4 marks)

Table 3.2
Criteria ATP Deoxyribonucleotide
Energy released/ absorbed
Catabolic/anabolic pathway

[Source: Final Examination, October 2023]

Q4. (a) An experiment was carried out to investigate digestion of starch and maltose by the salivary
amylase. Table 4 shows the description of each tube (1-6) as well as the results of Benedict’s
test and iodine test (+ indicates a positive result and – indicates a negative result).

Table 4

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Answer the following questions based on Table 3.

(i) Identify the solution (A or B) that is most likely a starch solution. (1 mark)

(ii) Explain why tube 1 gives a negative result while tube 2 gives a positive result in
Benedict’s test. (3 marks)

(iii) Identify TWO (2) control sets (tube 1-6) of this experiment. (2 marks)

(iv) Explain the effect of pH on the enzyme activity in tube 6. (5 marks)

(b) State the term that best matches each of the following statements.

(i) An additional site of an enzyme where molecule binds and affects the activity of the
active site. (1 mark)

(ii) An organic nonprotein molecule that binds to the enzyme to activate it. (1 mark)

(iii) The minimum quantity of energy required for a chemical reaction to occur. (1 mark)

(iv) Binding of a substrate can affect the ability of other active sites to bind to the
substrate. (1 mark)