Anand Institute of Higher Technology

B
Department of Biotechnology

Monthly Test-I March 2023

Year/Sem: II/IV Course Title: Industrial Enzymology Course Code: BT3452

Duration: 90 min Date: 06/03/2023 Maximum Marks: 50 marks

Instructions:
1. Before attempting any question paper, be sure that you got the correct question paper.
2. The missing data, if any, may be assumed suitably
3. Use the sketches wherever necessary
Ques
tion Question Mark BTL CO
No
Part A (5×2 = 10 Marks) Answer all Questions
1. Define enzyme. 2 L1 CO1

2. Discuss the difference between chemical catalyst and biocatalyst (enzyme). 2 L1 CO1

3. What are the assumptions in Lock-and-key model? 2 L1 CO1

4. What is the role of entropy in catalysis? 2 L1 CO1

5. Outline the concept of active site and energetic of ES complex formation. 2 L2 CO1

Part B (2×13 = 26 Marks)

Explain the following

6. a. (i) Principle of catalysis. 13 L5 CO1
(ii) Role of entropy in catalysis.
or
How the enzyme commission developed a system of classification and its
b. 13 L4 CO1
recommendations on nomenclature.

(i) Explain any three reactions involving oxidoreductase.

7. a. 13 L5 CO1
(ii) Explain the Strain and transition state Mechanism of enzyme action
or
b. Write in detail about bioenergetics in a living cell. 13 L6 CO1

Part C (1×14 = 14 Marks)

8. a. Explain with neat diagram the various models of enzyme action on substrate. 14 L6 CO1

or
State the significance of activation energy in enzyme catalyzed reaction based on
b. 14 L5 CO1
transition state theory and explain in detail about transition state theory.

Subject Staff HOD