BT34

USN 1 M S
(Autonomous Institute, Affiliated to VTU)
(Approved by AICTE, New Delhi & Govt. of Karnataka)
Accredited by NBA & NAAC with ‘A+’ Grade

SEMESTER END EXAMINATIONS – APRIL 2023

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Program : B.E. - Biotechnology Semester : III
Course Name : Biochemistry Max. Marks : 100
Course Code : BT34 Duration : 3 Hrs

Instructions to the Candidates:

 Answer one full question from each unit.

UNIT - I
1. a) Explain the reducing property of glucose. Explain why sucrose is a CO1 (06)
non-reducing sugar.
b) Classify monosaccharides based on number of carbon and functional CO1 (06)
groups with relevant examples and structures.
c) Discuss the different secondary structures of proteins. Interpret the role CO1 (08)
of various bonds in stabilizing the secondary structure of proteins.

2. a) Illustrate and explain anomers, epimers and stereo isomers with suitable CO1 (06)
examples.
b) Classify amino acids based on ‘R’ group giving one example for each CO1 (06)
class with structure.
c) Illustrate and explain the Watson and Crick model of DNA and its salient CO1 (08)
features.

UNIT - II
3. a) Describe the different types of enzymes specificities with relevant CO2 (08)
examples.
b) Define Co-enzymes. Explain the mechanism of action of NAD and FAD as CO2 (07)
co-enzymes in enzyme catalyzed reactions.
c) Compare the advantages of enzymes over chemical catalyst. CO2 (05)

4. a) Classify enzymes according to the IVB classification giving one example CO2 (08)
for each class. Explain the major class oxidoreductase.
b) Describe the salient features of active site of an enzyme. CO2 (06)
c) Illustrate the Fischer’s Lock and Key model and explain its limitations. CO2 (06)

UNIT - III
5. a) Describe the sequence of reactions in the preparatory and payoff phase CO3 (10)
of Glycolysis. Calculate the energetics in aerobic conditions.
b) Explain the oxidative reactions of Pentose Phosphate pathway. Add a CO3 (10)
note on the significance of the pathway in various tissues.

6. a) Define gluconeogenesis and list the key gluconeogenic precursors. CO3 (08)
Explain the bye pass reactions of Gluconeogenesis.
b) Explain Glyoxylate cycle. Add a note on its significance. CO3 (06)
c) Analyze the catabolic fate of Pyruvate in aerobic and anaerobic CO3 (06)
conditions.

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 BT34
UNIT- IV
7. a) Illustrate and explain the organization of Fattyacid synthase multi CO4 (08)
enzyme complex.
b) Discuss the two strategies for head group attachment in phospholipid CO4 (06)

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synthesis.
c) Explain saturated and unsaturated fatty acids with examples. Write a CO4 (06)
note on PUFA and its importance.

8. a) Discuss in detail the steps in the β oxidation of fatty acids. Calculate the CO4 (10)
energetic of palmitic acid oxidation.
b) Describe how fatty acids are activated in the cytosol and then CO4 (10)
transported to the mitochondrial matrix via carnitine shuttle.

UNIT - V
9. a) Explain the following : i) Feedback regulation. ii) Salvage synthesis of CO5 (15)
nucleotides. iii) Explain the role of Aspartate Tranaminases.
b) Different excretory forms of nitrogen. CO5 (05)

10. a) Discuss about the biosynthesis of any one non essential aminoacid. CO5 (05)
b) Explain the denovo biosynthesis of Pyrimidine nucleotides. CO5 (05)
c) Describe in detail the sequence of reactions in urea cycle. Add a note on CO5 (10)
its regulation.

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