Determination of total Ash and Water Soluble

Ash

Name:
M. Bilal Tariq
Reg. No:
19-ARID-4699
Section:
T Morning
Submitted to:
Dr. Rai Muhammad Amir
Sbject:
Sugar Technology ( FT-502)
Date:
07-04-2022
Institute of food and nutrition sciences

PIR MEHR ALI SHAH

ARID AGRICULTURE UNIVERSITY RAWALPINDI
PAKISTAN
2022
Determination of total Ash and Water Soluble

Ash

Determination of Total Ash

Procedure:

 Weigh accurately about 5g of sample in a tart or platinum dish.

 Char the material carefully completely in a burner and transfer the ash to a muffle furnace and ash

temperature 550+- 10 C for 30 minutes until the ash free of carbon.

 Heat the dish again 550+- to 10 C for 30 minutes and cool in a dessicator and weigh.

 Repeat this process of heating for 30 minutes and cooling in a dessicator and weigh until the

difference between two successive weighing is less then 1mg.

 Record the lowest weight.

Note:

 Preserve the dish containing this ash for the determination of acid in a soluble ash.

 Alternative method of determination of total ash is by using a programmable muffle furnace that

allow a gradual increase in temperature than charring by a Bunsen burner and inserting the

sample into the furnace at 550 C.

 The charring techniques is often prone to losses an you can have super heating of sample as they

enter the furnace causing them to explode and loss sample and contaminate the surrounding

sample.

 It is very quick but not as accurate the programmable muffle furnace.

 It is also safer to use a programmable furnace for the analyst handling crucible at 550 C.
  Instead of Bunsen burner, hot plates can also be used for charring techniques.

Calculation:

Total Ash (% on dry weigh) = (w 2 – w1) * 100*100 / (w1 – w) * ( 100 – M)

W1= weight in g of silica dish + sample

W2= weight in d of silica dish + ash

W= weight in grams of empty silica dish

M= Moisture % of the sample

Determination of Water Soluble Ash

 Transfer the ash with the aid of about 25ml distilled water into a beaker cover with the glass and

boil for 5 minutes.

 Filter through as ash less filter paper whatmin No.42 and its equivalent.

 Collect the filtrate into a 150ml beakers, wash the filter paper 4-5 minutes with hot water until the

filtrate no longer turns red litmus-blue and collect the washing in the same beaker.

 Dry the ash less paper with residues in an oven and transfer to muffle furnace and ignite at 530 C

for 2 hours.

 Cool in a dessicator and way 3 times repeat the process still the difference into consective

weighing is less than 1 mg.

 Record the lowest weight.

Calculations:
Water soluble ash % by weight= A- B

Where;

A= total ash % by weight

B= water sluble ash % by weight

Water soluble ash of total ash = water soluble ash/ total ash * 100