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Experiment Report No: 6_______ Section: CAS-O2-201A_________

Experiment Title: Aldehydes and Ketones_________ Date & Time: May 12. 2023_____
Name: Alan Rex C. Godio Jr._________________ Date Performed: May 12. 2023__
Course: BS-BIOLOGY________________________ Date Submitted: May 12. 2023___

OBJECTIVE:
● Students should be differentiate between ketone and aldehydes using qualitative
analysis

MATERIALS/ CHEMICALS:

Stopper Schiff’s reagent

Dropper Fehlings’ solution
Test tube 5 % NH3
Boiling tube 10 % NaOH
Water bath 2,4- dinitrophenylhydrazine
Thermometer 0.3 M AgNO3
Measuring cylinder Iodine in Kl solution
Benzaldehyde propane unknown Y Ethanal

PROCEDURES:
PART A: BRADY’S TEST

1. Place approximately 1 mL of 2,4-dinitrophenyl hydra

2. Place approximately 1 mL of 2,4-dinitrphenylhydrazine into a test tube and add 5 drops of

ethanal and shake the test tube

3. Heat it in a water bath at 60 – 70 degrees Celsius

4. Observed the formation of a precipitate

5. Repeat step 1 and 2 with benzaldehyde, propane, and unknown Y

6. If there is no precipitate, add 2 mL of distilled water.

7. Record the observation.

PART B: FEHLING’S TEST

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1. Place approximately 1 mL of ethanal, benzaldehyde, propnane and unknwn Y in 4
separate test tubes.

2. Add 2 mL of Fehlings’ solution in ethanal

3. Shake the test tubes gently.

4. Repeat the steps with benzaldehyde, propane and unknown Y

5. Heat the mixture in the hot water bath for 15 – 20 minutes.

6. Record the observation

PART C: TOLLEN’S TEST

1. Prepare Tollens’ reagent by adding one drop 10% NaOH t 2 mL of 0.3 AgNO3 in a boiling
tube

2. Add 5% NH3 dropwise until the precipitate dissolves

3. Place approximately 1 mL of ethanal, benzaldehyde, propane, and unknown Y in separate

test tubes.

4. Add 1 mL of Tollens’ reagent to each test tubes and shake the mixtures gently.

5. Allow the mixtures to stand for 3 minutes

6. If there is no change, warm the mixture in a water bath at 60 to 70 degrees Celsius for 5
minutes.

7. Record the observations

PART D: SCHIFFS’ TEST

1. Place approximately 1 mL of ethanal, benzaldehyde, propanone, and unknown Y in

separate test tubes.

2. Add 1 to 3 drops of Schiffs’ reagent into each of the test tubes.

3. Shake each test tube gently and note the color change.

4. If any of the compounds does not dissolve, stopper the test tubes and shake it vigorously
until an emulsion is formed.

5. Record your observation.

PART E: IODOFORM TEST

1. Place 10 drops of the iodine in KL solution into 3 mL of distilled water in a boiling tube.

2. Add 5 drops of ethanal into the boiling tube and shake gently.

3. Add 10% NaOH to the boiling tube drop by drop until the color of the iodine fades.

4. Allow it to stand for 2 to 3 minutes. If no precipitate forms, warm the boiling tube in a
water bath at 60 to 70 degrees Celsius.

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5. If the color of iodine disappears, add more iodine in Kl solution until the color of iodine is
retained.

6. Record your observations.

7. Repeat the steps with benzaldehyde, propanone and unknown Y.

DRAWING/DIAGRAM:

OBSERVATIONS & RESULTS:

Brady’s Fehling’s Tollen’s Test Schiff’s Test Iodoform

Test Test Test

Ethanal Yellow Brown Green Pink Light Yellow

Benzaldehyde orange Dark blue Colorless Light Pink White

Propanone Y-orange Blue Brown Pink Light Yellow

unknown Y Y-orange Blue Colorless Pink Yellow

CONCLUSION:

The conducted tests above such as Brady’s test, Fehling’s test, Tollen’s test, Schiff’s
test, and Iodoform test are the experiments that can differentiate ketones from aldehydes.
Fehling test, if the liquid turns blue it means that it is a negative test. While, if it turns red
then it is a positive test. The same with other test, the color will change depending from
their reaction and that will be utilized to differentiate it.