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Rabbit Anti- Phospho-Lyn (Tyr397)

磷酸化膜相关蛋白酪氨酸激酶Lyn抗体

Catalog Number: bs-3257R

Host: Rabbit

Target Protein: Rabbit Anti- Phospho-Lyn (Tyr397)

Concentration: 1mg/ml

Cross Reactive Human,Mouse,Rat (predicted：Human,Mouse,Rat,Chicken,Pig,Rabbit)

Species:
Predicted MW: 58

IR:Immunogen NE(p-Y)TA

Range:
Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4067

Swiss Prot: P07948

Source: KLH conjugated synthesised phosphopeptide derived from human Lyn around the
phosphorylation site of Tyr397-NE(p-Y)TA

Storage: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Background:

VALIDATION IMAGES

Sample: Paraformaldehyde-fixed, paraffin

Lane 1: Mouse Placenta tissue
lysates
Lane 2: Mouse Embryo tissue
lysates
Lane 3: Mouse BV2 cell lysates
Lane 4: Mouse Raw264.7 cell
lysates
Lane 5: Human A431 cell lysates
Lane 6: Human Raji cell lysates
Primary: Anti-Phospho-Lyn
(Tyr397) (bs-3257R) at 1/1000
dilution
embedded (mouse lung tissue);
Antigen retrieval by boiling in
sodium citrate buffer (pH6.0) for
15min; Block endogenous
peroxidase by 3% hydrogen
peroxide for 20 minutes; Blocking
buffer (normal goat serum) at
37°C for 30min; Antibody
incubation with (Phospho-Lyn
(Tyr397)) Polyclonal Antibody,
Unconjugated (bs-3257R) at 1:400
overnight at 4°C, followed by
 Secondary: IRDye800CW Goat operating according to SP
Anti-Rabbit IgG at 1/20000 Kit(Rabbit) (sp-0023)
dilution instructionsand DAB staining.
Predicted band size: 56 kDa
Observed band size: 58 kDa

Sample: Tissue/cell: human glioma tissue;

Placenta (Mouse) Lysate at 40 ug 4% Paraformaldehyde-fixed and
Lung (Mouse) Lysate at 40 ug paraffin-embedded;
Primary: Anti-Phospho-Lyn Antigen retrieval: citrate buffer (
(Tyr397)(bs-3257R)at 1/300 0.01M, pH 6.0 ), Boiling bathing
dilution for 15min; Block endogenous
Secondary: IRDye800CW Goat peroxidase by 3% Hydrogen
Anti-Rabbit IgG at 1/20000 peroxide for 30min; Blocking
dilution buffer (normal goat
Predicted band size: 56kD serum,C-0005) at 37℃ for 20 min;
Observed band size: 63kD Incubation: Anti-Phospho-
Lyn(Tyr397) Polyclonal Antibody,
Unconjugated(bs-3257R) 1:200,
overnight at 4°C, followed by
conjugation to the secondary
antibody(SP-0023) and
DAB(C-0010) staining

Antigen: bs-3257P, 0.2ug/100ul Paraformaldehyde-fixed, paraffin

Primary: Antiserum, 1:500, embedded (rat spleen tissue);
1:1000, 1:2000, 1:4000, 1:8000, Antigen retrieval by boiling in
1:16000, 1:32000; sodium citrate buffer (pH6.0) for
Secondary: HRP conjugated Goat- 15min; Block endogenous
Anti-Rabbit IgG(bs-0295G-HRP) at peroxidase by 3% hydrogen
1: 5000; peroxide for 20 minutes; Blocking
TMB(C-0024) staining; buffer (normal goat serum) at
Read the data in 37°C for 30min; Antibody
MicroplateReader by 450nm. incubation with (Phospho-Lyn
(Tyr397)) Polyclonal Antibody,
Unconjugated (bs-3257R) at 1:200
overnight at 4°C, followed by
operating according to SP
Kit(Rabbit) (sp-0023)
instructionsand DAB staining.

PRODUCT SPECIFIC PUBLICATIONS

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or

diagnostic applications.