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  • Types of Microscopes

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    Gul Afsha
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    Light microscopes use visible light and have a maximum useful magnification of 1000x, allowing study of living cells, while electron microscopes use electron beams for much higher resolution up to 100,000x magnification but require killed and preserved cells. The document outlines the key differences between light microscopes, which have lower resolution but allow viewing living cells, and electron microscopes, which have much higher resolution but require fixed cells and describes their general uses and capabilities.

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    Microscopes

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    Types of microscopes

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    Light microscopes use visible light and have a maximum useful magnification of 1000x, allowing study of living cells, while electron microscopes use electron beams for much higher resolution up to 100,000x magnification but require killed and preserved cells. The document outlines the key differences between light microscopes, which have lower resolution but allow viewing living cells, and electron microscopes, which have much higher resolution but require fixed cells and describes their general uses and capabilities.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    14 views1 page

    Types of Microscopes

    Uploaded by

    Gul Afsha
    Light microscopes use visible light and have a maximum useful magnification of 1000x, allowing study of living cells, while electron microscopes use electron beams for much higher resolution up to 100,000x magnification but require killed and preserved cells. The document outlines the key differences between light microscopes, which have lower resolution but allow viewing living cells, and electron microscopes, which have much higher resolution but require fixed cells and describes their general uses and capabilities.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    Types of microscopes

    (1) Light microscope


    (2) Electron microscope

    Light microscope (LM)

    • Limited in resolution, with useful magnification limited to about 1000x

    • But, must use for study of living cells

    Magnification:

    Increase of apparent size of object

    Resolution:

    Ability to separate close objects

    Contrast:

    Differences in intensity

    Electron Microscopes

    – Use beam of electrons instead of light

    – Much higher resolution (1000x) than LM

    – Magnification of up to 100,000x

    • Can see organelles (“little organs”) within cells

    – But, must kill & preserve cells to look at them

    Types:

    – Scanning electron microscope (SEM)

    • Study cell surface

    Take pictures of surface of specimen

    – Transmission electron microscope (TEM)

    • Study internal cell structure

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