TO PREPARE AND EVALUATE HAIR REMOVING LOTION

Assignment of

COSMETCS SCIENCE (PRACTICAL)

PHS CC-6108

Session 2022-23

Supervisor Submitted by

Prof. Vandana Soni Mr. Prabhat Kumar Uikey

(Y20150071)
Mrs. Sapna Jain Mr. Rajendra Ahirwar
Choudhary (Y20150073)
Ms. Rakhee
Mrs. Priyanka Jain (Y20150074)
Ms. Sarjana Raikwar B. Pharm. 6th Sem

Department of Pharmaceutical Sciences

Dr. Harisingh Gour Vishwavidyalaya, Sagar, (M.P.)
(A Central University)
Object- To Prepare and evaluate hair removing lotion

Ingredient table:
FORMULA 1: Depilatory Cream

Coconut alcohol 0.65ml Emollient / Thickener

Calcium thioglycolate 0.54g Cream base
Calcium hydroxide 0.7g Regulate pH
Sodium lauryl sulphate 0.002g Surfactant
Sodium silicate 0.343g Speeds hair cutting
Perfume q.s. For fragrance
Water 10 ml ----------------

FORMULA 2: Powder Depilatory

Calcium thioglycolate 2.0g Powder base
Calcium hydroxide 2.31g Regulate pH
Strontium hydroxide 0.89g Regulate pH
Sodium lauryl sulphate 0.15g Surfactant
Hydroxymethylcellouse 0.01g Thickening agent
Magnesium carbonate 4.52g Bulking agent
Perfume 0.03ml For fragrance

FORMULA 3: Epilating Wax

Rosin 5.2g Wax base and provide
adhesiveness
Beeswax 2.5g Wax base
Paraffin 1.7g Provide moisture
Petrolatum 0.5g Provide moisture
 Perfume 0.01ml For fragrance

FORMULA 4: Depilatory Lotion

Barium sulphide 3g Facilitates eradication of
body hairs
Glycerol 6ml Humectant
Iodine water 1ml Antiseptic
Cream base: -------------
Stearic acid 0.65g Thickening agent
Propylene glycol 0.15ml Humectant
Methyl paraben 0.001g Preservative
Cetyl alcohol 0.1g Prevent cream base from
separating into oil and
liquid
Triethanolamine q.s. Stabilizes emulsion
Water q.s. -------------------

Theory-
Hair removing creams are also known as Depilatory cream. A
"depilatory" can truly be categorised as a cosmetic, since it beautifies
by removing unsightly hair from certain parts of the body.
The term depilatory must therefore be reserved for chemical means of
hair removal from skin (in particular superfluous hair occurring on the
face, legs, etc) without causing any injury to the skin. It is definitely
different from other methods of hair removal, which includes:
• Mechanical removal of hair
• Destruction of hair by electrolysis, by use of laser energy or
diathermy
• Shaving
Chemistry of hair & its removal
Hair mainly consists of amino acids, mainly cysteine and most of
these amino acids containing sulphur constitute a group of proteins,
known as Keratin.
 Electrolysis is a suitable method for permanent removal, but is
slow and costly
 The other electrical methods consist of the use of galvanic
current and high frequency current. The galvanic method
consists of passing direct current through one or more special
needles inserted at the hair follicle and destroy hair permanently.
The method is slow, but yields good result
 The removal of hair by x-rays is very dangerous, as these rays
also destroy the skin/tissue surrounding the hair, and thus, this
method should not be recommended
Regarding hair removal, two topics deserve special mention:
a) Disguising
b) Epilation
Disguising- Instead of superfluous hair being removed, they can be
disguised in such a way that they become less noticeable and thus
blend with underlying skin.
Epilation – It simply consists of trapping the hair in a viscous
adhesive mass and then using physical force to pull the trapped fibre
from its body anchor.
 Some benefits can be seen by "hot" processes where warm wax
is allowed to cool on the skin. It is also customary to add
benzocaine, a local anaesthetic, to certain formulation to reduce
the pain.
 The ingredient basis for cosmetic epilating products mainly
includes rosin (colophony), bees wax and viscous sugar
 composition. They form a sticky mass, usually grips the hair but
has less adhesion to the skin.
Depilation- Depilatories are the preparations used for degradation of
the superfluous hair chemically without affecting the skin.
 It removes the hair at the neck of the hair follicle and thus has
advantage over razor shaver which removes hair on a level with
the surface of the epidermis
 The depilatories act by degrading the hair Keratin. As it is
known that Keratin is sensitive to the action of strongly alkaline
aqueous solutions and reducing agents, most of the depilatories
consist of such agents
An Ideal Depilatory Should Be:
 Non-toxic and non-irritant to skin
 Fast and efficient in action, causing depilation within 5 minutes
 Preferably odourless
 Should be stable upon storage.
 Non-staining/damaging to clothing.
 Cosmetically elegant.
Formulation Of Depilatories:
Chemical depilatories are sold in the form of liquids, pastes and
powders. Typical ingredients of a chemical depilatory include:
 Alkaline reducing agents
 Perfumes
 Emulsifiers
 Emollients
 Thickening agent
 Humectants
Alkaline reducing agents: Depilatory preparations usually contain an
alkaline reducing agent as their active component. These agents will
cause the hair fibres to swell and cleave the cystine bridges between
adjacent polypeptide chain, causing degradation of hair.
E.g.: Sulphides, Stannite’s etc.
Perfumes: Most of the alkaline reducing agents, including salts of
thioglycolic acid, have usually an odour of their own, hence, the use
of perfumes is almost a necessity in depilatory products.
E.g.: Aromatic alcohols, Rose, Saffron etc.
Emulsifiers: Emulsifiers are used for cosmetic elegancy and
potential irritancy. E.g.: Sodium lauryl sulphate, Ethylene oxide
ethers etc.
Emollients: The common emollients are mineral oils and paraffins.
Thickening agents: The synthetic thickening agents such as methyl,
hydroxy ethyl or carboxy methyl cellulose are used. These are used to
make paste of requisite consistency.
Humectants: Humectants are incorporated to prevent quick drying on
the skin. E.g.: Glycerine, Sorbitol, Propylene glycol etc.

EVALUATION OF DEPILATORY EFFICACY:

1) Tensile kinetics method: In this method, stress decay caused
by disulfide bond reduction is measured, using commercial
instruments such as tensile strength tester, an optical diameter
gauging system and an electro balance. The time required to
reduce the stress supported by hair by 95% (T95%) was shown
to correlate to in-vivo hair removal rate in commercial products.
2) HPLC Method: This method distinguishes between
thioglycerol, thiolactic acid and thioglycolic acid. It results a
yellow derivative, permitting HPLC detection at 464 nm. The
 procedure is most suitable for aqueous preparation, including
o/w creams and lotions.
3) Thermo mechanical method: In this method a thermo
mechanical analyzer is used to measure the time at which a hair
bundle, under constant stress and immersed in depilatory, begins
to stretch. The test is carried out under isothermal conditions
and indicate good precisions, which can be correlated with
results on animals.
MARKETED PRODUCTS

Hair Removal Cream Hair Removal Powder

Hair Removing Wax Hair Removing Lotion

The Marketed Products are sold under different kinds of products, such
as lotion, cream, powder, wax etc.
Procedure:
Preparation Of Cream Base –

 Firstly, weighed all the ingredients properly

 Take steric acid and cetyl alcohol in China dish and melt it over
the water bath (oil phase)
 Now in another container, take triethanolamine, methyl paraben
& propylene glycol and melt it in water bath (water phase)
 Now add water phase drop wise into oil phase with continuous
stirring
Preparation Of Hair Removal Lotion-

 Firstly, take prepared cream base in a beaker

 Now add iodine water in cream base and mix it properly
 Now add glycerol into the prepared mixture, again mix it
properly
 Lastly add barium sulphide in the prepared mixture
 Stir the prepared mixture continuously for better mixing
 Lastly add 1-2 drops of rose fragrance in the preparation
 Maintain the pH of the preparation up to 12.0
Evaluation:
Name of Test Specification Observation
Preparatio
n
Appearance Viscous Confirmed
liquid
Texture Smooth Confirmed
Colour White Confirmed
Spreadability Easily Confirmed
spreadable
pH 10.0-12.0 Confirmed
Result:
The Preparation and evaluation of hair removing lotion has been
performed.
References:
1. Singh S.P., Nigam V. “COSMETIC SCIENCE” First Edition 2021,
Thakur Publication Pvt. Ltd., Page No. –

2. Khar R.K., Vyas S.P., Ahmad F.J. & Jain G.K., “LACHMAN/
LIBERMANS The Theory and Practice of Industrial Pharmacy” 4th
Edition 2016, CBS Publication and Distributor, Page No-

3. Pathak K.& Vidya A. “A Textbook of Cosmetic Science, Concept

and Principle” First Edition 2018, Nirali Prakashan, Page No.