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  • Calibration Methods

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    Jun Ming
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    Calibration methods involve either external standard calibration, where standards of known concentrations are used to create a calibration curve, or internal standard calibration. For internal standard calibration, a constant amount of a similar substance is added to blanks, standards, and samples. A calibration curve is then created by plotting the ratio of the analyte signal to the internal standard signal versus the analyte concentration. An ideal internal standard is absent from samples, has a similar but distinguishable signal from the analyte, and provides correction for matrix effects and instrument variations.

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    Calibration methods

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    Calibration methods involve either external standard calibration, where standards of known concentrations are used to create a calibration curve, or internal standard calibration. For internal standard calibration, a constant amount of a similar substance is added to blanks, standards, and samples. A calibration curve is then created by plotting the ratio of the analyte signal to the internal standard signal versus the analyte concentration. An ideal internal standard is absent from samples, has a similar but distinguishable signal from the analyte, and provides correction for matrix effects and instrument variations.

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    5 views13 pages

    Calibration Methods

    Uploaded by

    Jun Ming
    Calibration methods involve either external standard calibration, where standards of known concentrations are used to create a calibration curve, or internal standard calibration. For internal standard calibration, a constant amount of a similar substance is added to blanks, standards, and samples. A calibration curve is then created by plotting the ratio of the analyte signal to the internal standard signal versus the analyte concentration. An ideal internal standard is absent from samples, has a similar but distinguishable signal from the analyte, and provides correction for matrix effects and instrument variations.

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    Calibration

    methods

    Dr Noorfatimah Yahaya,
    Department of Toxicology
    Advanced Medical and Dental Institute
    Universiti Sains Malaysia
    Why must we calibrate?
    External standard calibration
    Internal standard calibration
    An internal standard (I.S.) in analytical chemistry is a
    substance similar to the analyte that is added in a constant
    amount to the blank, the standards, and the samples.
    Internal standard : added in a constant amount to all
    samples, blanks and calibration standards.
    Calibration curve: plot the ratio of analyte signal to the
    internal- standard signal as a function of the analyte
    concentration of standards.
    Finding a suitable substance :
    ◦ The internal standard must be known to be absent from the
    sample matrix
    ◦ Provide a signal that is similar to the analyte signal in most way
    but sufficiently different so that the two signals are
    distinguishable by the instrument
    ◦ Eg: Li for the determination of Na or K in blood serum
    Standard addition method

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