‫تردد بكرتيا الزائفت الزجناريت‬

‫ملرضى التهاب اجملاري البوليت يف مدينت العمارة‬

‫‪Pseudomonas aerugiosa‬‬
‫‪33‬‬

‫‪2009\4\1- 2\1‬‬
‫‪28‬‬

‫‪Pseudomonas aerugiosa‬‬

‫‪Pseudomonas aeruginosa‬‬

‫‪467‬‬ ‫‪2011‬‬
Amoxycillin , Gentamycin , Cefotaxaime , Carbenicillin ,
Aztreonam , Ciprofloxacin , Piperacillin , Cefazolin.
Norfloxacin , Amikacin
6
100
pseudomonas 94 0
aerugiosa

Introduction

(1)

(2)

(3)

468 2011
 Pseudomonas aerugiosa

(4)
P.aeruginosa

(5) Nasocomial infection)

P.aeruginosa

(6,7)
P.aeruginosa

Haemolysin
Lipase Protease
(8,9) Exotoxin phospholipase C
P.aeruginosa

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 in- vitro

(Materials & Methods)

(Equipment & Apparatus) -1

p.aeruginosa
(Centrifuge

(Autoclave )

(Hot plate magnetic

stirrer

( Incubation)
37
(Spectrophotometer)

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45

(Water distilator)

(Electric oven)
200

(Sensitive balance)

(Millipore filter unit)

(Light microscope)

(pH meter)

(Culture media) :- 2

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 P.aeruginosa
(11, 10)
(Blood base agar)
( Brain heart infusion agar)
(Agar-agar medium)
(Urea base agar )
(Simon S citrate agar )
(MacConkey agar )
(Muller Hinton agar )
(Nutrient agar &broth )

Difico (U.S.A )

3
(11,10)
(Antibiotic disc) 4

25 , Gentamycin 25 Amoxycillin
Ciprofloxacin ,
Carbenicillin , 30 Cefotaxaime , 25
Aztreonam , 30 Cefazolin. 100

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 Piperacillin , 30 Cefotaxime , 30
Amikacin 10 Norfloxacin , 100
30
Standard Strain 5
(ATCC 27853 ) P. aeruginosa

Different materials 6

(11,10) API 20 E
Patient s samples 7

2009\4\1- 2\1)

10

P.aeruginosa
(11,10)

Isolation&Diagnosis
Macroscopic appearance) 1

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 (11,10)
(Microscopic appearance) :- 2
(Gram
s stain)
(11,10)
(Biochemical tests) 3
Urease tests)
(Oxidase tests)
(Catalases tests)
Indol production tests)
Methyl red tests)
(Voges proskauer tests)
(Citrate utilization tests)

(API 20 E Techneqe) :- 4
P.aeruginosa

(Oil )

37
24

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 Antibiotic
susceptibility

12 Disc diffusion tests

MacFarland tube
10 1)

(13,11)
(Results&Discussion)
P.aeruginosa
Non
lactose fermenter

Pyocyanin
(15,16) Obligately
P.aeruginosa
Single Motile
(0.7- 2.5) polar flagellum

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 (16,4)

P. aeruginosa

1
17,16,4
(API 20 E)

16

33 33
28
P.aeruginosa 18
31
P.aeruginosa 5
51.1

p. Nosocomial infection
%90-60 aeruginosa
%11.4 19

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%9.3
(%8.7)
20 (%8.6)

P. aeruginosa

21

P. aeruginosa 2
Cefazolin Amoxycillin
( %90)30 (%100)33

22

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 P.aeruginosa
Cefotaxime Carbencillin
23 (%72)23
(%76) Cefotaxime
Gentamycin
(%46)15
(BroadSpectrum)
(24 p. aeruginosa
P. aeruginosa
(%94) 31
Aztreonam Amikacin (%92)30
(%88) (%78)
9 2
P. aeruginosa
Ciprofloxacin %76 (%90) Amikacin
(%66.4) Aztreonam 25

26

p. aeruginosa

p. aeruginosa 27

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(%28.7) (%19.9.) Perfloxacin Ofloxacin
Antibiotic
Combination
28

P. aeruginosa 1

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 P. aeruginosa 2

100 33 25 Amoxycillin
90 30 10 3 30 Cefazolin
72 23 28 10 100 Carbencillin
72 23 28 10 30 Cefotaxime
46 15 54 18 10 Gentamycin
22 7 78 26 10 Norfloxacin
18 6 82 27 100 Piperacillin
12 4 88 29 25 Ciprofloxacin
8 3 92 30 30 Aztreonam
6 2 94 31 30 Amikacin

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 Frequency of Pseudomonas aerogniosa
isolates of urinary tract infection( UTI)
patients in Al-Emarha city

Ali.A.Saihod
Technical Institute - Amara

Abstract :-
In this study One hundred urine samples obtained
from patients suffering from urinary tract infection(UTI) ,
whom admitted to AL sadr generl Hospital for the period of
1/2 – 1/4/2009 ,in Emarha city.
All urine samples were cultured on MacConkey agar and
Blood Agar base , then Macroscopic , Miacroscopic
appearance and Biochemical tests were done to diagnose the
purity isolates of p .aeruginosa , which found with( 33%)
while there were (28)isolates obtained.
Results of susceptibility to c to antibiotics
Amoxycillin , Gentamycin , Cefotaxaime , Carbenicillin ,
Aztreonam , Ciprofloxacin , Piperacillin , Cefazolin.
Norfloxacin , Amikacin ) showed that this bacteria had
resistant mode ranged between 6% to 100% , while it was
also having wide range of sensitivity ranged between 0% to
94% for Antibiotics which listed .
The study should be recommending to use antibiotic
combination to reduce antibiotic resistance of bacteria and
to treat patients with UTI

485 2011