Agenda Item 65/36 - Annexure - 32

BBIT303L Genomics and Proteomics L T P C

3 0 0 3
Pre-requisite BBIT202L, BBIT202P, BBIT204L, BBIT204P Syllabus version
1.0
Course Objectives
1. Develop knowledge on the basics of Genomics and Proteomics and their versatile
applications.
2. Integration of the Genomics and Proteomics approaches in various biological models.
3. Technical skills and knowledge development on versatile techniques.
Course Outcomes
1. Analyse the principle of gene and protein sequencing.
2. Compare genomics and gene expression profiling.
3. Distinguish the principle of functional and structural genomics.
4. Identify proteins based on their functional and structural properties.
5. Analyse protein interaction networks.
6. Apply genomic and proteomic patterns in industrial and medicinal diagnostics and
Treatment.
Module:1 Gene Structure and Sequencing 8 hours
Sequence complexity - Introns and Exons, Genome structure in viruses and prokaryotes,
Organelle genomes and nuclear DNA in eukaryotes, Chain terminator sequencing,
Automated DNA sequencing, High throughput sequencing, Alternate DNA sequencing
methods.
Module:2 Comparative Genomics and Global Expression Profiling 8 hours
Protein evolution by exon shuffling, Comparative genomics of prokaryotes and eukaryotes,
Horizontal and lateral gene transfer, Traditional approaches to expression profiling, Blobal
analysis of RNA expression - Spotted DNA arrays, Printed oligonucleotide chips, Data
acquisition and analysis, Serial analysis of gene expression, Massively parallel signature
sequencing.
Module:3 Functional and Structural Genomics 8 hours
Functional genomics by systematic gene knockout, Genome wide random mutagenesis, Use
of
chemical mutagens and phenocopy libraries, Determining gene function by sequence
comparison, Structure prediction methods, Domain fusion method for functional annotation.
Module:4 Proteome Sequencing and Post-Translational Modification 5 hours
Gel electrophoresis (1DE and 2DE), Liquid chromatography and mass spectrometers for
protein and peptide analysis, Routes in proteome analysis, Protein digestion techniques,
Protein identification by mass fingerprinting, Analysis of posttranslational modifications,
Signal peptide
cleavages, Tagging of proteins with chemical and genetic approaches.
Module:5 Protein Mining 4 hours
Sequence analysis by tandem mass spectrometry, Databases and algorithms in protein
identification.
Module:6 Protein Expression Analysis I 4 hours
Comparative proteomics, Use of isotope tags, Yeast two hybrid systems,
Immunoprecipitation and western blot analysis, Shotgun identification of multiprotein
complex, Bait and reverse bait analysis.
Module:7 Protein Expression Analysis II 6 hours
Protein-Protein interaction, Identifying the protein interaction regions, Protein interaction
network, Sample enrichment for detecting protein modifications, Integration of different
algorithms to map protein modification, Glycoprotein analysis, Protein arrays, Intrinsically
disordered proteins and their importance in understanding disease processes.
Module:8 Contemporary Issues 2 hours

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 Agenda Item 65/36 - Annexure - 32

Total Lecture hours: 45 hours

Text Book(s)
1. Arthur M Lesk, Introduction to Genomics, 2nd Edition, 2017, Oxford University press,
United Kingdom.
Reference Books
1. Discovering genomics, proteomics and bioinformatics, by Malcolm Campbell A and
Laurie J Heyer, Ist Edition 2002, Cold Spring Harbor Laboratory Press, United States.
2. Principles of genome analysis and genomics, by SB Primrose and RM Twyman, 3rd
Edition, 2003, Blackwell publishing, USA.
3. Introduction to proteomics: Tools for the new biology, by Daniel C Liebler, Ist Edition,
2002, Humana Press, USA.
Mode of Evaluation: CAT, Assignment, Mini project, Quiz and FAT
Recommended by Board of Studies 18-02-2022
Approved by Academic Council No. 65 Date 17-03-2022

Proceedings of the 65th Academic Council (17.03.2022) 669