Agenda Item 65/30 - Annexure - 26

TBIT212L Proteomics L T P C
3 0 0 3
Pre-requisite TBIT103L, TBIT104L, TBIT105P Syllabus version
1.0
Course Objectives
1. Describe basics in the field of proteomics.
2. Classify various techniques that are used in the study of proteomics.
3. Illustrate biological importance of protein-protein interactions, modelling and protein
database and their clinical relevance.

Course Outcomes
1. Understand basics of proteomics.
2. Utilize various techniques in protein separations.
3. Choose different methods to identify proteins.
4. Explain importance of protein-protein interactions.

Module:1 Overview 6 hours

Overview of protein chemistry; Applications of proteomics; Functional proteomics in post-
genomic era; Experimental workflows; Gene-protein family links with examples; Human
proteome draft.
Module:2 Chromatography in Proteomics 7 hours
Multidimensional chromatography; NanoLC; Combined fractional diagonal
chromatography (COFRADIC); GCxGC-two dimensional gas chromatography; Hydrophilic
interaction liquid chromatography (HILIC); Strong anion exchange chromatography (SAX);
Strong cation-exchange chromatography (SCX); High performance thin layer
chromatography (HPTLC).
Module:3 Abundance-based Proteomics 6 hours
One dimensional and two dimensional 2-D gel electrophoresis, Difference gel
electrophoresis (DIGE), Mass spectrometry analysis of data; SELDI; Peptide mass
fingerprinting; Protein microarray.
Module:4 Structural Proteomics 5 hours
X-ray crystallography; Circular dichroism; Nuclear magnetic resonance; Plasmon
Resonance; Tagging of proteins; Epitope mapping; Protein sequencing.
Module:5 Post-translational Modification 6 hours
Techniques
Phosphorylation, ubiquitination, acetylation, nitration, glycosylation, sumoylation, disulphide
linkage; Signal peptide cleavages; Tagging of proteins with chemical and genetic
approaches.
Module:6 Protein-protein and Protein-DNA 7 hours
Interactions
Qualitative and quantitative proteome analysis; Short-gun proteomics for proteome profile:
Whole proteome and sub-proteome analysis; Expression proteome analysis: Isotope-
labeling
and label-free approaches; In vivo proteome analysis; Proteomic analysis of protein-protein,
and protein-DNA interactions; Identification of ligand-receptor pairing and transcriptional
regulators.
Module:7 Clinical Proteomics 6 hours
Proteomics in study of diseases; Storage, transportation and processing of clinical samples;
Proteomic analysis of body fluids; Immunohistochemistry; Western blotting; Data analysis;
Proteomic data interpretation via systems biology; Enhancement of omics data interpretation
by network modelling; Data pre-processing; Identification of differentially expressed proteins;
Network modelling for prediction of key regulators; Peptidomics, peptidomimetics,
glycoproteomics and glycopeptidomics.

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 Agenda Item 65/30 - Annexure - 26

Module:8 Contemporary Issues 2 hours

Total Lecture Hours 45 hours

Text Book
1. Proteomics: Principles, Techniques and Analysis, by Peter Wyatt, 1st Edition, 2018,
Syrawood Publishing House, USA.
Reference Books
1. Modern Proteomics: Sample Preparation, Analysis and Practical Applications, by Hamid
Mirzaei and Martin Carrasco, 1st Edition, 2016, Springer Publications, USA.
2. Introduction to Bioinformatics, by Arthur M. Lesk, 5 th Edition, 2019, Oxford University
Press, UK.
3. Principles of Proteomics, by Richard M. Twyman, 1 st Edition, 2013, Garland Science,
UK.
Mode of Evaluation: CAT, Assignment, Quiz and FAT
Recommended by Board of Studies 18-02-2022
Approved by Academic Council No. 65 Date 17-03-2022

Proceedings of the 65th Academic Council (17.03.2022) 212