T&J Minibox® CloudReadyTM

High-throughput Bioreactor System

Features & Technical Specifications

V 0.0.1.5 – 1.5L Vessel

T&J Bio-engineering (Shanghai) Co., LTD

No. 8 Mingnan Road, Songjiang District, SHANGHAI, 201611, P.R. China

www.tjxbio.com
 T&J Bio-engineering (Shanghai) Co., LTD

Fig.1 Overall dimensions

Fig. 2 Controller top view

2 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 3 Headplate layout

Fig. 4 Vessel internals (Fermentation Version)

3 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Item Data & Specifications

Category
Wetted materials Vessel Borosilicate glass
Head plate 316 SS
Agitator 316 SS
Baffles 316 SS
Sparger 316 SS
Vessel dimensions Nominal Volume (mL) 1500
ID (mm) 97
Height (mm) 223
Total Volume (mL) 1590
Working Volume (mL) 300-1100
Sparger Type drilled pipe
Size 11×Φ1mm
Impeller Type Rushton × 2+Pitched blade×1
Diameter (mm) 44
Clearance (mm) 44 (adjustable)
Power number 6
Headplate ports Pg 13.5 6
RTD 1
Feed (1x 3mm) 4
Sparger (4x 6mm) 1
Condensor Pg13.5×1
Agitation speed (rpm) 50~1500
Temperature Heating and cooling Peltier

Range (°C) 10-45

Accuracy (°C) ±0.2
Max RoC (°C/min) 1.0 (cooling from 37°C to 20 ℃)
pH Probe type Glass (K8 connection)
Range 0~14
Accuracy ±0.02
DO Probe type Polarographic (or Optical ECS, VP6 connection)
Range 0~150% (Air saturation at 1 atm)
Accuracy ±0.1%
MFC Gas type Air
Flow rate range 0-3000 sccm (0~2vvm)
Pumps Type Peristaltic pump head, precision stepper motor
Count 4*
Flow rate 0.03mL/min ~ 30 mL/min

*Optional 6 pumps

4 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 5 Skid dimensions (Depth = 700 mm)

Device and Data Management System (D2MS)

System requirements
Operation system 64-bit Windows 10 Professional or higher
Hard drive 100 GB or more, NVME SSD with RAID 1 recommended
RAM 16 GB (Increase if the customer uses MS SQL Server Enterprise, see below)
Ethernet Must be compatible with 100 Mbps to communicate with the control system.
Additional interfaces (e.g., WiFi) to connect to the internet for remote control.
A separate WiFi network is recommended for streaming reactor videos if used
heavily.
Software 1. Microsoft SQL Server Express 2017*
environment 2. LabVIEW Runtime 2018 SP1
(To be installed by 3. .Net Frameworks
T&J) 4. Internet time service
5. Python 3.6.2 32bit with scientific computation libraries
6. VNC Viewer

*Single database size is limited to 10 GB. Maximum memory use is limited to 1GB.

5 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Feature highlights

❖ Digital lab book ❖ Data Analysis

❖ Centralized management ❖ Workflow automation
❖ Distributed Control ❖ Advanced (programable) control

Combined into one package and deeply integrated with the hardware.

• Data-centric, modern, and fluid user interface. Data management, run-to-run

comparison
https://mp.weixin.qq.com/s/b2TdTyil5ru8nYbS6Kp9Vg
https://mp.weixin.qq.com/s/39nwMl2lgzWIBjtroJ4C9A
• D2MS WebAccess remote control and data visualization
https://mp.weixin.qq.com/s/JOrxLa_bwBA6RCAxRN28Cw
• 21 CFR Part 5 and GAMP Compliance:
Audit trail https://mp.weixin.qq.com/s/J0k1GixX79H6okSzH8GFag
https://mp.weixin.qq.com/s/en-MxE4ZbFIrCLiRhL2OSg
Data integrity https://mp.weixin.qq.com/s/Lg9AK9Kn3z8-aS09h2bScQ
User management https://mp.weixin.qq.com/s/OkJG2TVHJ2MqpOngLfDY8g
• Digital lab book, searchable operator action journal (operation log) and audit
trail
https://mp.weixin.qq.com/s/mRNnpYtaURChQqLwiy1bkA
• Remote monitoring with live video streaming
https://mp.weixin.qq.com/s/-7abDHFR-0ADW6ffsSMHpA
• Built-in DOE (Design of Experiments) with 3D visualization of design space
https://mp.weixin.qq.com/s/KjHCRuj6Pdnu5ivFud_Hzg
• “Golden batch”, replicating a successful run easily
https://mp.weixin.qq.com/s/58TrDa3ZKycEF5jwORh1cg
• Advanced process control using python-based user-defined functions (UDF)
https://mp.weixin.qq.com/s/CugbJWerNsXwMH-fakte-A
• Controlling a practically unlimited number of reactors under a unified user
interface
▪ Performing parallel operations by selecting multiple reactors
▪ Copying operations from one reactor to another
• Offline Sample management
▪ Time, person, type, and results are all linked to the specific experiment
run in a relational database.
▪ Taking samples (generating sample ID, not referring to the physical action
of sampling) and entering offline data for multiple reactors at once.
▪ Online-offline data are plotted in the same graph for visual examination
of the effect of operational changes on the process

6 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 6 D2MS “Parallel operation” on multiple reactors and multiple parameters

Fig. 7 D2MS “One-to-many” operational settings copying

7 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 8 Batch calibration of multiple sensors and pumps

8 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 9 User-configurable pump functions and advanced feeding strategies (pre-defined

functions, PDF) with graphical preview, operate with maximum confidence1

1
Function descriptions are not hard-coded. Can be changed to English

9 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 10 Advanced PID controller configurations (split range, nonlinearity)

Fig. 11 A familiar Windows PC based HMI with right-click menus for maximum efficiency.

10 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 12 Set point profiles (time series) with real-time visualization for DO, Feed (6x), pH and Temperature. Edit on PC for maximum
convenience, download to PLC for execution with highest reliability (limited number of time steps). Edit for one reactor and
instantaneously copy the settings to other reactors with a few clicks of the mouse. Import/Export as CSV files for future use, offline
editing, use in experiment reports, or use in other systems.

11 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 13 Multi-reactor, multi-parameter set point profiles (time series) with real-time visualization of progress. Executed on PC for
maximum flexibility with practically unlimited number of time steps. Import/Export as CSV files for future use, offline editing, use in
experiment reports or use in other systems, etc.

12 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 14 Automatic operation triggers based on time or process.

13 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 15 Detailed meta data for experiment can be used to search for historical runs or for data mining.
14 WWW.TJXBIO.COM
 T&J Bio-engineering (Shanghai) Co., LTD

Fig. 16 Key information of a run is displayed on the touch panel of each reactor, alongside real time process data.

15 WWW.TJXBIO.COM