NAME: DEL ROSARIO, RONNIE SANTIAGO

AGE: 35 SEX: Male DATE OF BIRTH: 03/25/1985

ADDRESS: - GERONIMO ST. MENZYLAND MOJON, CITY OF MALOLOS
PASSPORT NO. - HOSPITAL/LGU: Malolos LGU
SPECIMEN TYPE: Nasopharyngeal / Oropharyngeal LAB ID: 0040867 SPECIMEN ID: mlos-0000000200
swab
DATE AND TIME OF COLLECTION: 12/29/2020 15:00 DATE AND TIME OF RECEIPT: 12/29/2020 23:20
DATE AND TIME OF RELEASE OF RESULT: 12/30/2020 15:10
PATHOLOGY TEST PERFORMED

Molecular Diagnostic Test Result

SARS – CoV-2 (causative agent of Covid-19) virus detection by Real-Time Reverse Transcription Polymerase Chain Reaction

RESULT:

ASSAY INTERPRETATION
SARS-CoV-2 NOT DETECTED

METHOD: Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) was performed to test the presence of SARS-CoV-2 viral RNA. RNA
extraction was performed using an automated RNA extraction machine to purify viral RNA in the sample. FDA approved SARS-CoV-2 qRT-PCR reagent kits
were used to detect SARS-CoV-2 specific gene targets (RdRp/N/ORF1ab/NSP-3). Amplification and detection were done using BIORAD CFX96 Real-Time PCR
machine with positive and negative controls included in each run to confirm validity and accuracy of results.

TEST: Qualitative detection of SARS-CoV-2, the causative agent of COVID-19, extracted from nasopharyngeal &oropharyngeal swab samples.

LIMITATION: Pre-analytical variables such as sample collection, storage, handling and transport may affect the quality of results. The detection of viral RNA
is dependent on the viral load if the sample was collected early and/or late in the course of infection. PCR results should not be used as the sole basis of
patient treatment management decision. PCR results should be interpreted by a physician in conjunction with review of patient’s history and clinical signs
and symptoms.

QUALITY ASSURANCE: MMDC-JVMDL is comprised of trained laboratory personnel in performing molecular diagnostic assays and passed the external
proficiency testing conducted by the Department of Health – Research Institute for Tropical Medicine.