Int J Artif Organs 2014 ; 37 ( 8 ): 608

XLI Annual ESAO Congress, 17-20 September 2014, Rome, Italy

Poster Sessions

Poster Session 1 – Part 1, 609

Renal Assist 1, P1-P9
Renal Assist 2, P10-P18
Cardiac Assist 1, P19-P26
Cardiac Assist 2, P27-P34
Implants and Robots, P35-P40

Poster Session 1 – Part 2, 617

Renal Assist 3, P41-P50
Renal Assist 4, P51-P61
Cardiac Assist 3, P62-P73
Cardiac Assist 4, P74-P82

Poster Session 2 – Part 1, 626

Computer Modeling, P83-P92
Biomaterials 1, P93-P100
Biomaterials 2, P101-P108
Tissue Engineering 1, P109-P116
Tissue Engineering 2, P117-P123

Poster Session 2 – Part 2, 634

ECMO and Instrumentation, P124-P133
Transplantation, Ethics & Psychology, P134-P141
Artificial Organs, P142-P149
Nanobiotechnology & Drug Delivery, P150-P158
Regenerative Medicine, P159-P164

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608 © 2014 Wichtig Publishing - ISSN 0391-3988

Int J Artif Organs 2014 ; 37 ( 8): 609-643
Posters: XLI ESAO Annual Congress, 17-20 September 2014, Rome, Italy
Methods: Electrospun nanofibrous membranes were applied to construct a
hepatoma tissue sheet due to the fact that microstructure of nanofibrous mem-
branes are very similar to the natural extracellular matrix (ECM). Mouse fibro-
blasts, which are an integral component of tissue architecture, were co-cultured
with Hepa 1c1c7 to make a better microenvironment than mono-culture ones.
We measured the hepatic albumin expression level to determine cellular func-
tion and examined the effect of fibroblasts on the hepatocellular morphology
and spheroids. The difference between the construction of co-culture and mo-
no-culture cell sheets were checked by histological studies and immunofluo-
rescent analysis.
Results: Our research indicates that co-culturing mouse hepatocellular carci-
noma and fibroblasts on electrospun nanofibrous membranes was able to make
a hepatoma tissue for investigation of liver cancer treatments.
Conclusions: For future work, we have a plan to apply this investigated method
for other cancer types. The developed tissue also will be assembled with a mi-
crochannel to create a tumor-on-a-chip.
P148
OPTIMIZED OPTICAL GLUCOSE READER FOR TRANSCUTANEOUS GLU-
COSE MEASUREMENTS
M. Bazhenova, J. Fischerb, R. Tharb, M. Rumplerd, M. Hajnsekd, K. Mottaghya,
I. Klimantc
a
RWTH Aachen, Germany; bPyroScience GmbH, Aachen, Germany; cTU Graz,
Austria; dJoanneum Research, Graz, Austria
Aim: The development of a single-port closed-loop system aims for continuous
glucose monitoring, insulin dose calculation and continuous insulin infusion as
a “all-in-one” artificial pancreas system. The glucose sensor is based on two
luminescent dyes with different excitation wavelengths grafted on standard in-
sulin infusion sets, showing a glucose-sensitive and O2-sensitive luminescence,
respectively. Here we present an optimized optical reader for reliable transcuta-
neous read-out of the sensors.
Methods: The system is divided into an integrated optoelectronic reader that
is located on the skin over the infusion set with the optical sensor layers and a
separate signal processing unit. This design allows miniaturizing the reader for
maximum convenience of the patient. Bright SMD LEDs with custom collimating
optics and large area photodiodes are combined with optical interference filters
for spectral discrimination between the glucose and the reference O2 channel.
Results: The sensor measures only Ø25 mm at 7 mm height, allowing conve-
nient continuous wearing. The reader can be integrated in standard 90° infusion
sets with 0.4 mm steel cannula. The sensor is characterized in terms of signal
intensity, channel cross-talk, optical background and signal to noise ratio. Inter-
stitial glucose and O2 in animal models are compared to blood values.
Conclusions: The optimized optical glucose reader shows promising perfor-
mance valuable for the further development of the innovative single-port artifi-
cial pancreas system.
P149
INFLUENCE OF CELL REMOVAL TREATMENT ON DERMIS MECHANICAL
BEHAVIOUR
M. Terzinia, C. Bignardia, C. Castagnolib, E.M. Zanettic, G. Catapanod,
A.L. Audeninoa
a
Politecnico di Torino, Torino, Italy; bSkin Bank – Trauma Center, Torino, Italy;
c
University of Perugia, Perugia, Italy; dUniversità della Calabria, Rende (CS), Italy
Aim: Knowledge of the mechanical behaviour of skin is required by various
medical disciplines, such as dermatology, surgery and traumatology. More spe-
cifically, this work was focused on one specific skin layer: the dermis. The aim
was to verify the influence of the decellularization treatment on its properties.
Methods: Skin specimens were subjected to uniaxial static tests performed
with the Bose Electroforce® 3200. Experimental data were represented with
engineering and real time stress-strain curves. Descriptive parameters were
identified from stress vs. strain curves, and they were subsequently compared
through multivariate analysis of variance to determine the influence of the speci-
men cut orientation and of duration of the decellularization treatment.
Results: Dermis samples which had been decellularized for 5 to 6 weeks, exhib-
ited mechanical properties comparable with native dermis. The ultimate tensile
strength and the maximum Young’s modulus was shown to be considerably
higher in real time curves than in engineering ones: 3-4 times and up to 7 times
respectively for ultimate tensile strength and Young’s modulus.
Conclusions: Real-time curves should be used when modeling dermis be-
haviour, while engineering curves should be confined to comparative analysis,
where they are able to provide indications with a higher repeatability and a sim-
pler experimental set up.
640 © 2014 Wichtig Publishing - ISSN 0391-3988
Nanobiotechnology & Drug Delivery
P150
MODIFIED COATING OF LIPOSOME ENCAPSULATED HAEMOGLOBIN
WITH POLYETHYLENE OXIDE
H. Tabesha,c, Gh. Amoabedinyb, A. Ramedania, F. Ahmadia, K. Mottaghyc
a
Faculty of New Sciences and Technologies; bDepartment of Chemical Engi-
neering, University of Tehran, Tehran, Iran; cInstitute of Physiology, ECC Lab.,
RWTH University, Aachen, Germany
Aim: Liposome Encapsulated Haemoglobin (LEH) has been already introduced
as an artificial oxygen carrier nanoparticle with the potential substitution ca-
pability for red blood cells. However, the synthesis process of current LEH
yields low haemoglobin (Hb) loading due to the instability of LEH encapsulation.
Therefore, the surface coating of LEH with a stable polymer would be beneficial.
Methods: Bovine Hb is encapsulated by hydrating a dry-film lipid (comprising
0.4 mM Cholesterol and 0.6 mM Dipalmitoylphosphatidylcholine) through a phos-
phate buffer solution blended with glutamine antioxidant. In the next step, the
size of synthetized particles is decreased to nano-range using a high-pressure
homogenizer. The synthesis process is modified by the insertion of polyethylene
oxide (PEO) through the solution during the cascade of homogenization.
Results: Coated LEH with 2.5, 5, 10 and 40% w/w of PEO present an increase
of the total Hb loading yield of 27 ± 3, 33 ± 2, 39 ± 2 and 45 ± 2% respectively,
compared to non-coated LEH with a loading yield of 20 ± 1%. On the contrary,
the size of coated LEH increase gradually from 107 ± 3 nm to 228 ± 3 nm by
raising the concentration of PEO from 2.5 to 40% w/w while non-coated LEH
shows the size of 98 ± 3 nm.
Conclusions: Coating of LEH with PEO during its synthesis process increases
its stability, and subsequently, enhances its efficiency by elevating the total Hb
loading yield. However, the effect of the increase of particle size should be fur-
ther investigated.
P151
ARTIFICIAL OXYGEN CARRIER LIPOSOME-ENCAPSULATED HEMOGLO-
BIN AS THE PLASMA OXYGENIZER
A. Kawaguchia, H. Ohbab, Kakiuchib, D. Fukumotob, M. Haidac, H. Tsukadab
a
Tokai University School of Medicine, Kanagawa, Japan; bHamamatsu Photon-
ics, Hamamatsu, Japan; cTokai University, Junior School of Nursing and Medical
Technology, Tokyo, Japan
Aim: Since liposome-encapsulated hemoglobin (LEH) has a low specific gravity
similar to plasma and different O2-affinity from that of red blood cell (RBC), it is
currently impossible to measure, but calculate/assume O2 contents in the blood
of animals infused with LEH.
Methods: We examined effects of LEH administration in the blood content of
oxygen containing molecules, including CO2, O2 and CO in non-human primates
undergoing steady-state gas inhalation study. Plasma and whole blood radio-
activity were sequentially monitored in the arterial blood while animals were
successively breathing [15O]-labeled gases ([15O]CO2, [15O]O2 and [15O]CO). The
monkeys received base-line measurement, underwent occlusion of the middle
cerebral artery, and received LEH with different O2-affinity and in various doses
(n = 18), empty liposome (n = 4) or saline (n = 7) (T2). The [15O] steady-state res-
piration tests were repeated immediately following reperfusion (T3) and 3-hours
thereafter (T4).
Results: While radioactivity of [15O]CO2 did not change regardless of infusates,
[15O]CO increased in the plasma fraction in a LEH-dose-dependent manner,
[15O]O2 increased in the plasma fraction in an O2-affinity-dependent manner in
the plasma fraction. These levels remained elevated for the following 6 hours,
which was parallel to the calculation based on O2 binding characteristics.
Conclusions: These results suggest that LEH serves as a plasma oxygenizer to
increase O2 content in a unit of blood and may account for benefits of LEH on
ischemia/reperfusion of organs and/or tissues.
P152
LIPOSOME-ENCAPSULATED HEMOGLOBIN ENHANCES CHEMOTHERAPY
TO SUPPRESS METASTASIS IN MICE
C. Murayama, A. Kawaguchi, A. Kamijo, K. Naito, K. Iwao, H. Tsukamoto,
K. Yasuda, Y. Nagato
Tokai University School of Medicine, Tokyo, Japan
Aim: Liposome-encapsulated haemoglobin (LEH) with high O2-affinity (P50O2 =
10 mmHg, h-LEH) was reported to enhance tumor radiosensitivity. We hypoth-
esize that targeted O2 delivery to tumor hypoxia by h-LEH may also enhance
chemotherapy.