Specification 1,05459.0500 Tryptic Soy Broth Casein-peptone soymeal-peptone broth for microbiology (According harm. EP/USP/JP and ISO) Specification ‘Appearance (cleamess) lear ‘Appearance (color) yellowish brown pHvalue (25 °C) 14-78 ‘Stability test (7 days, room temperature) clear ‘Typical composition (litre): Pepton from casein 17.0; Peplon from soymeal 3.0; D(+)-Glucose monohydrate 25; Sodium chloride 5.0; - Potassium hydrogen phosphate 2.5, ‘Growth promotion test in accordance withthe harmonised method of EP, USP and JP. Specification Inoculum on reference medium 10-100 (Staphylococcus aureus ATCC 6538 (WDCM 90032)) Inoculum on reference medium (Bacilus 10-100 subilis ATCC 6633 (WOCM 00003)) Inoculum on reference medium 10-100 (Pseudomonas aeruginosa ATCC 9027 (won 09026), Inoculum on reference medium (Escherichia 10-100 coli ATCC 6739 (WOCM 00012) Inoculum on reference medium 10-100 (Streptococcus pneumoniae ATCC 6301) Inoculum on reference medium (Salmonella 10-100 typhimurium ATCC 14028 (WOCM 00031)) Inoculum on reference medium 40-100 (Staphylococcus aureus ATCC 25923 (wooM 09034) Inoculum on reference medium 40-100 (Staphylococcus epidermidis ATCC 12228 (wo 09036) Inoculum on reference medium (Candida 10-100 saboicans ATCC 2091 (WDCM 00055)) Inoculum on reference medium (Candida 10-100 albicans ATCC 10231 (WOCM 00054) Inoculum on reference medium (Aspergilus 10-100 brasiliensis (formerly A. niger) ATCC. 16404 (WDCM 00053)) ‘ference medium: Tryatic Soy Agar (Escherichia cof, Staphylococcus aureus, Streptococcus pneumoniae, Bacils subtls, Pseudomonas ‘eruginasa, Salmonela typhimurium, Staphylococcus epidermidis), SABOURALD-2%-Glucose Agar (Candida albicans, Asperiglus brasiliensis) Specification {Growth /18-2th at 30-35°C (Staphylococcus visible growin ‘aureus ATCC 6538(WOCM 00032)) Merck KGaA, Frankfurter Strate 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 1 of 2 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000Specification 1,05459.0500 Tryptic Soy Broth Casein-peptone soymeal-peptone broth for microbiology (According harm. EP/USP/JP and ISO) ‘Growth [16-2th at 30-35°C (Bacilus subilis visible growih [ATCC 6633 (WDCM 00003) Growth /18-24h at 0-35°C (Psoudomonas visible growth ‘seruginosa ATCC 9027 (WDCM 00026) Growth /18:24h at 30-35°C (Escherichia col visible growth [ATCC 8739 (WDCM 00012), Growth /18-24h at 90-35°C (Streptococcus visible grow ‘pneumoniae ATCC 630%) Growth /18-24h at 30-35°C (Salmonelia___vsible growth ‘typhimurium ATCC 14028 (WOCM 00031)) Growth /3 days a120-25°C (Staphylococcus visible growth ‘aureus ATCC 6538 (WOCM 00032)) Growth /3 days a120-25°C (Staphylococcus visible grow ‘aureus ATCC 25923 (WDCM 00034)) {Growth 3 days st 20-25°C (Staphylococcus visible grow epidermidis ATCC 12228 (WDCM 00036)) Growth 13 days st 20-25°C (Bacilus subils visible growth ‘ATCC 6633 (WDCM 00003) (Geowth 5 days at 20-25°C (Candida albicans visible grown [ATCC 2091 (WDCM 00055) Growth /5 days a1 20-25°C (Candida albicans visible grow ATCC 10291 (WDCM 00054)) Growth /5 days 31 20-25°C (Aspergilus visible growth brasiliensis (formerly A. niger) ATCC. 16404 (WDCM 00083)) ‘The casein used for processing the Pepton from casein as contained is beeing saurced from heathy cows and declared good for human ‘consumption. The casein is acid precipitated. Therefor, this product does not come under the scope of Ph.Eur. 5.2.8 For detaiis, please request our BSE-certfcale, Stefanie Fischer Response laboratory rs aly Sonal ‘This document has been produced electronically and is valid without a signature. Merck KGaA, Frankfurter Strae 250, 64293 Darmstadt (Germany): +49 6151 72.0 Page 2 of 2 EMD Millipore Corporation -a subsidiary of Merck KGaA, Darmstadt, Germany 400 Summit Drive, Burlington, MA 01803, USA, Phone +1 (781) 533-6000