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4.0 ACCOUNTABILITY
Sr. Manager Quality Assurance
5.0 PROCEDURE
5.1 Keep all the glassware, used in microbiology, in the cabinet provided for the same.
5.2 Do not use this glassware in the chemical laboratory.
5.3 Whenever glassware is used for microbiological analysis, dispose the inoculated media, at the end of the incubation period, as per the procedure
described in SOP for disposal of media.
5.4 After disposal of the media, dip all the infected glassware in 3% v/v Dettol solution for 30 minutes.
5.5 Rinse with water and dip it in a 3% v/v solution of teepol for one hour.
5.6 Remove the glassware from the teepol solution and brush all the glassware.
5.7 Rinse the glassware continuously with running tap water till there are no traces of teepol solution left and, finally, rinse with purified water.
5.8 After washing, dry all the glassware in an oven at 120ºC for 60 mins.
5.9 Keep the pipettes and Petri dishes in their respective stainless steel containers.
5.10 Sterilize the glassware by autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
5.11 Once in a month, dip the glassware, in the chromic acid mixture, overnight.
5.11.1 While handling chromic acid mixture, use safety goggles and rubber gloves, remove the glassware carefully from the chromic acid mixture.
5.11.2 Rinse the glassware thoroughly with tap water, as the glass tends to absorb chromic acid, and finally, rinse with purified water.
5.11.3 Check the pH of the final rinse by means of a pH paper to confirm the absence of traces of cleaning agent.
Ankur Choudhary is India's first professional pharmaceutical blogger, author and founder of pharmaguideline.com, a widely-read pharmaceutical
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