Getting Started with your FLEXMAP® 3D

Table of contents:
About the FLEXMAP 3D

• FLEXMAP 3D Details
• Support for your FLEXMAP 3D
• Warranty for the FLEXMAP 3D
• Getting your final license keys for your software
• Warranty information for your Dell PC
• Upgrading software modules at a later date
• Service and training solutions for the FLEXMAP 3D
• Useful information sources
• FLEXMAP 3D quick guide

Luminex®  | 1
 About the FLEXMAP 3D
The FLEXMAP 3D is an enhancement of our current flow cytometric-based platform, the Luminex
100 and 200. The new system can acquire samples twice as fast as the Luminex 200 for High
Throughput Screening applications and can read a combination of 500 multiplexed microspheres. In
combination with your analytes of interest this translates into a 500 plex assay.
The FLEXMAP 3D also incorporates numerous usability enhancements:
• Automatic Adjusting Probe
• Probe height settings library maintained and loaded for each new batch
• Calibration Kits for weekly system calibration
• Color Coded caps and CDs containing importable target values
• Performance Verification Kits for daily system verification
• Color Coded caps and CDs containing importable target values
• Reagents for Classification, Reporter and Fluidics parameter verification
• xPONENT 4.0 software
• Simple, easy to navigate user interface
• Built in maintenance, calibration, verification and start-up routines
• Flexible, adaptable user interface options for lab specific customization
• Optional Security module allows Group and Individual permissions settings
• 21 CFR part 11 Compliant module upgrade available
• LIS and Automation Compatible upgrades available
• Intra-well Normalization, Quantitative, Qualitative, and Allele Call Analysis
• Built-in auto-start of Third Party Analysis Packages
• Extended System Performance Verification Reports

The FLEXMAP 3D system combines differentially dyed fluorescent microsphere sets with an innovative
instrument design to take bead-based multiplexing technology to the next dimension. Dual sampling
channels, increased injection rate and 384-well plate compatibility deliver faster read times, while other
features such as automated probe adjustment, straightforward routine maintenance operations and an
intuitive automation and LIS-compatible software interface simplifies operation.

The combination of these features make FLEXMAP 3D the platform of choice for flexible, simple
high-throughput multiplex testing.

2 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

How is FLEXMAP 3D faster?
Improvements that significantly reduce assay runtime:
• Dual sample fluidics paths and increased syringe injection speed for faster sample injection rate
• Optimized sample flow for higher multiplex requirements
• Fast switching, low dead volume, multi-port valve to direct sample flow stream
• Detailed DSP algorithms to handle the high rate of real-time resultant data

Test (50 µl well volume) Read Time* Tests/hour

96 well (100 plex, 2500 beads per well) 18 min 32,000
384 well (100 plex, 2500 beads per well) 1 hour 15 min 30,700
96 well (500 plex, 2500 beads per well) 45 min 64,000
384 well (500 plex, 2500 beads per well) 2 hour 15 min 85,000
*Read times measured on 4 dual channel instruments. Actual results may vary.

384 Well Plate Compatibility

The FLEXMAP 3D is compatible with 96 and 384-well plate formats and offers sample plate heating
control for thermally sensitive applications. These features combined with the foil piercing sampling
probe provide reliable temperature control over a range of 35C to 60C.

Automation/LIS Compatibility
The new xPONENT 4.0 software controls the FLEXMAP 3D system and offers interfacing options for
laboratory information systems (LIS) as well as automation. An optional swivel base for the system
further simplifies integration with third-party liquid handlers.

Simplified Maintenance
The FLEXMAP 3D system has multiple features which simplify start-up, shutdown and maintenance
operations and greatly enhance usability for HTS applications.
• Automated probe height adjustment
• Automatic calibration for MicroPlex™ and MagPlex™ microspheres, and enhanced PMT settings
• Calibration and verification kits with color coded reagents and CD with importable reagent target values
• System initialization routine combines system warmup, washes, calibration and verification into
one simple daily startup routine

Luminex®  | 3
 How did Luminex build a 500 plex?
The FLEXMAP 3D system works in the same basic manner as the LX200 analyzer. Mixtures of
internally dyed xMAP microspheres are used in an assay, each having been prepared to bind to
a specific analyte of interest. After the assay has been performed, the mixture of microspheres is
aspirated into the system and each microsphere is individually excited by a red and green laser.
The xMAP microspheres, impregnated with three dyes,excite at the same red laser wavelength and
fluoresce at three seperate wavelengths. Monitoring the relative intensity of the three signals allows
the system to discriminate up to 500 different microsphere sets. The green laser is then used to
excite any surface associated reporter molecules, normally in the form of phycoerythrin, and the
resulting fluorescence is measured to determine the amount of analyte present.

What types of Microspheres can be used?

All current Luminex microsphere products are compatible with the FLEXMAP 3D easing conversion
of current Luminex microsphere based research products to the new system. For commercially
available assay kits, check with the assay kit manufacturer to confirm assay compatibility and
available support with the FLEXMAP 3D system. If running an assay using xMAP Technology as an
in vitro diagnostic, the assay must be run according to the instructions provided with the assay.

Reagent Available Regions

MicroPlex™ Microspheres 1 - 150
MagPlex™ Microspheres 1 - 150
SeroMAP™ Microspheres 1 - 100
xTAG™ Microspheres 1 - 100
LumAvidin™ Microspheres 1 - 100

What does the new bead map look like?

The new three-dimensional bead map is a matrix of the emitted fluorescence of the three internal
dyes on the x, y, and z axis. It is a 3D graph with ellipsoid targets for each region. The original 1-100
bead map is the base of the 500 plex bead map with the new regions emerging from it as the third
dye is added in increasing amounts.

4 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

How do the new microsphere sets compare to the
previous sets?
Each xMAP microsphere line, such as the MicroPlex Microspheres and MagPlex Microspheres,
is sourced from highly controlled progenitor microsphere lots to ensure homology of microsphere
functionality and surface chemistry. The same progenitor microsphere formulations and equivelant
dyeing proceses are used to create regions for the expanded FLEXMAP 3D map. Microsphere
integrity is maintained within the expanded map as the combinations of internal dyes do not affect
microsphere functionality or surface chemistry.

Luminex®  | 5
 FLEXMAP 3D Details
Support for the FLEXMAP 3D
For questions or problems you have while working with your FLEXMAP 3D system please contact
our Technical Support Group at (1-877) 785-2323, +1-512-381-4397 outside of the U.S or email
support@luminexcorp.com. Our Technical Support Group can answer questions about your
instrument, software, and provide guidance in system operation. For assay kit troubleshooting
please contact the kit manufacturer.

Warranty for the FLEXMAP 3D System

The FLEXMAP 3D system comes with a one year warranty. After the warranty year we strongly
suggest that end-users purchase a service agreement to cover system maintenance and repairs.
About nine months after the instrument purchase a Luminex Representative will contact you to initiate
this discussion. Please see the “Luminex Maintenance and Service Agreements Options” sheet for
more information about service contracts on the platform.

Custom/Homebrew Assay Support

For more information about developing assays on the FLEXMAP 3D see www.luminexcorp.com/
products/customassay.html for links to protocols, an extended bibliography, reagents, training, FAQs,
and much more.

Software License Keys

The FLEXMAP 3D comes with a variety of software upgrade modules:
• 21 CFR part 11 Compliant Capabilities
• Laboratory Information System Interface Module
• Automation Interface Module
• Security Module
• Allele Call Analysis Module

6 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

Upon initial installation, the xPONENT 4.0 software will open with a 90 day trial license. Prior to the
end of this 90 day period, you must contact Technical Support to setup a permanent access key for
the modules of software purchased with your system.

Luminex Technical Support is available to users in the U.S. and Canada by calling 1-877-785-BEAD
(-2323). Users outside of the U.S. and Canada can contact us at +1-512-381-4397. Inquiries may also
be sent by email to support@luminexcorp.com.

In order to assign your license key, Technical Support will need information about your Luminex
system. Please have the following information available:
• The serial number of your Luminex system

Additionally, Technical Support will need information provided in the xPONENT licensing export file.
To create this file:
• Open the Luminex xPONENT software.
• Select “Admin” from the toolbar.
• Select the “Licensing” tab.
• Click the “Export to File” button, which creates a text attachment that can be saved wherever is
convenient for you to retrieve it.
• Email this attachment to support@luminexcorp.com.

Once you have purchased your FLEXMAP 3D, you can later upgrade any of the optional modules by
contacting your Luminex partner or Luminex sales representative or by contacting Luminex Technical
Support.

Luminex®  | 7
 Warranty for the Dell PC that is included with the
FLEXMAP 3D System
Dell provides a limited warranty with PCs shipped from Luminex. Upon receipt of the Dell PC that is
associated with your Luminex System, please complete the warranty transfer described below. This
will ensure the newly acquired PC is in your name and that you will receive technical support from
Dell for the remainder of your warranty period.

Required Items: Service Tag Number and Express Service Code (ESC) located directly on the Dell PC.

Dell PC Information
Service Tag Number
Express Service Code

Detailed instructions and a link to the Dell PC Warranty Transfer are located on the Luminex
Technical Support website, http://luminexcorp.custhelp.com, search for keyword “Dell”. Click on
the PC Warranty Transfer link to be redirected to the Dell Warranty Transfer Tutorial and to the Dell
Warranty Transfer Website.

Warranty Transfer Form Instructions

Current Customer Information

1. Complete the Current Customer Information by placing an X in any *REQUIRED field, except the
Service Tag and ESC fields.
2. Enter the Service Tag and ESC into the appropriate fields.

New Customer Information

1. Complete all *REQUIRED fields in this section with YOUR information. This is how your system
will be registered with Dell.
2. Once all *REQUIRED fields are completed, hit “Submit”. *Please ensure that you enter a correct
e-mail address. If, for any reason, your request fails to process immediately, you will be notified
at the e-mail address entered in the New Customer Information when Dell has completed the
request.
3. After submission, a successful ownership transfer will result in a confirmation message listing the
Service Tag numbers transferred.

8 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

4. If your transfer request fails to process automatically, you will receive a failure message. All
failures are automatically routed to the appropriate group within Dell for research and response.
You will be notified when your request has been completed or if additional information is needed.
*Please do not submit your request multiple times.

NOTE: If you have any questions regarding the transfer of this Service Agreement, please contact
Dell Customer Service at 1-800-624-9897. The warranty transfer form is also located at the following
URL: http://support.dell.com/support/topics/global.aspx/support/kb/en/document?dn=1011182&c=us
&l=en&s=gen&cs=#1ststep. On that page, click either the Transfer of Ownership Form (United States
Only) link or the Unit Location Transfer (International) link, according to your location.

If you need assistance finding the service tag number and ESC information on your system, click on
the “What are the Dell™ Express Service Code and Service Tag, and where can I find them?”
link located on the transfer form.

Luminex®  | 9
 Upgrading Software Modules at a Later Date
Your system will come with the modules of Luminex xPONENT 4.0 Software Solutions that you
purchased at the time of sale. You will have the option of later upgrading with additional software
modules should the need arise.

The System Requirements and Upgrade Checklist will assist in determining that your system
components are upgrade ready and that your customized xPONENT 4.0 Upgrade Kit contains your
required items.

Please contact Luminex Technical Support if you need assistance in locating any of the requested
information.

Luminex Technical Support is available to users in the U.S. and Canada by calling 1-877-785-BEAD
(-2323). Users outside of the U.S. and Canada can contact us at +1-512-381-4397. Inquiries may also
be sent by email to support@luminexcorp.com.

Once you have completed the System Requirements and Upgrade Checklist provide it to your sales
representative who will use it to create your quote. Please reference this quote number when you
order your customized xPONENT 4.0 upgrade kit.

10 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

System Requirements and Upgrade Options Checklist
Please record the following information. If you need assistance locating any particular Item or if you
have any questions, Luminex Technical Support will be happy to assist you.

1. Contact and location information:

Name:

Phone Number:

Address 1:

Address 2:

City, State, Zip:

Partner through which you purchase Luminex systems:

2. Please provide the serial number of your Luminex System: FM3DD

3. Is the FM3D with xPONENT 4.0 being used or will be used in a clinical/ diagnostic lab? Yes  No 
• This will help determine the modules you will or will not need.

4. Do you want to upgrade to the 21 CFR Part 11 Module? Yes  No 

• The xPONENT Software provides the user the opportunity to enable the 21CFR Part 11 module
for regulatory compliance. This module maintains data integrity through the use of electronic
signatures. The 21CFR Part 11 module contains the Basic Security module.

5. Do you want to upgrade to the Laboratory Information System (LIS) Module? Yes  No 
• This module enables xPONENT 4.0 software to transmit and receive data from Laboratory Information
Systems (LIS) and Laboratory Information Management Systems (LIMS) using an ASTM file format.

6. Do you want to upgrade to the Automation Interface Module? Yes  No 

• The xPONENT 4.0 software will facilitate the communication/integration between front-end
automation devices and Luminex FM3D system. The front-end automation provider can build the
required driver through an Application Programming Interface (API) which will be supplied with the
Automation Interface Module.

7. Do you require additional seat licenses (beyond 3 that are standard)? Yes  No 
If so, how many additional seats do you require? ____________
The maximum number of seat licenses per Luminex system is 15.

Luminex®  | 11
 Other Resources
xPONENT 4.0 Built-in Help System (xPONENT 4.0 Software)

FLEXMAP 3D Hardware User’s Manual (Online)

xPONENT 4.0 Software User’s Manual (Online)

FLEXMAP 3D and xPONENT 4.0 Quick Guide (Included here)

FLEXMAP 3D Calibration Kit Insert (on CDs with Calibration Kit)

FLEXMAP 3D Performance Verification Kit Insert (on CDs with Performance Verification Kit)

Luminex Website:

www.luminexcorp.com – All Hardware, Software and User manuals, FAQs, Protocols, Support information

Luminex Technical Support is available to users in the U.S. and Canada by calling 1-877-785-BEAD
(-2323). Users outside of the U.S. and Canada can contact us at +1-512-381-4397. Inquiries may also
be sent by email to support@luminexcorp.com.

Service and Training Solutions for FLEXMAP 3D

As xMAP™ technology by Luminex Corporation continues to revolutionize the medical, life sciences
and diagnostic industries with cutting edge technologies, the Luminex Technical Operations Team
continues to expand our Service and Training organizations by offering a broad menu of options and
extending our global reach to serve the needs of our customers. This growth will continue to enable
exceptional service agreement coverage to ensure that instruments are maintained and serviced
appropriately, and the latest training to accurately and effectively utilize the instruments to their
maximum capabilities.

12 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

FLEXMAP 3D Education Services by xMAP University
With the purchase of a FLEXMAP 3D system, you will receive a training credit that must be used
within 90 days of delivery of the instrument. With this credit, the following classes will be provided for
2 individuals:

• Technology Training – web based

• FLEXMAP 3D Software and Maintenance Training – web based
• FLEXMAP 3D End User Training – one day instructor led held at a Luminex facility with the op-
tion for onsite training

The Luminex Training Team will dispatch* a Technical Trainer to your facility at an agreed upon date
to provide two individuals with the technical training necessary to operate the instrument. Our trainer
will provide:

• Necessary demonstration reagents for the training

• Quick Guides of the training class
• Training Manuals

xMAP University also provides outstanding opportunities for continuing education on assay
development, technical troubleshooting and more. Visit www.xmapuniversity.com to explore:

• A full course menu

• The New Luminex Virtual Lab
• Luminex Social Networking Community
• Video FAQs
• And More!
*Travel advisories provided by the FAA are strictly followed

Luminex®  | 13
 Service Solutions for FLEXMAP 3D
Instrument and Assay Packages* Instrument Only Packages*

Platinum 360 Maintenance and Support Package Platinum Maintenance and Support Package
• Unlimited visits for emergency repair • Unlimited visits for emergency repair
• Travel, parts and labor • Travel, parts and labor
• Two preventive maintenance visits per year • Two preventive maintenance visits per year
• Onsite 24 hour response time • Onsite 24 hour response time
• Priority scheduling • Priority scheduling
• Product Updates and Modifications • Product Updates and Modifications
• Software Support • Software Support
• As needed on-site assay support • 24x7x365 remote support
• 24x7x365 remote support
Gold Maintenance and Support Package
Gold 360 Maintenance and Support Package • Unlimited visits for emergency repair
• Unlimited visits for emergency repair • Travel, parts and labor
• Travel, parts and labor • One preventive maintenance visit per year
• One preventive maintenance visit per year • Onsite 48 hour response time
• Onsite 48 hour response time • Priority scheduling
• Priority scheduling • Product Updates and Modifications
• Product Updates and Modifications • Software Support
• Software Support • 24x7x365 remote support
• As needed on-site assay support
• 24x7x365 remote support Bronze Maintenance and Support Package
• One preventive Maintenance visit per year
• 20% discount on replacement parts for
emergency repair
• 24x7x365 remote support

* Available in North America and the following countries in the EU: Austria, Belgium, Denmark, Finland, France, Germany, Greece,
Ireland, Italy, Luxembourg, Netherlands, Portugal, Spain, Sweden and the United Kingdom

For questions concerning Luminex’s Training or Service options, please contact the Technical
Operations Sales Team at serviceandtraining@luminexcorp.com or call +1-512-219-8020.

14 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

xPONENT® 4.0 and FLEXMAP 3D™
Instrument Quick Guide
The FlexMAP 3D instrument that contains fluidics, optics, sheath fluid, and an platform for microtiter
plate movement. See the User’s Manual for information. Refer to the FLEXMAP 3D Hardware User’s
Manual, xPONENT 4.0 Software User’s Manual and reagent package inserts for complete instructions
and safety information, including electrical, mechanical, radiation, biohazard and chemical safety
information.

- not user servicable - user servicable at routine intervals - user servicable weekly
 Use the below diagram as guidance for part accessibility:
System Preparation - Fluidics and Probe Height
1. Power FLEXMAP 3D system on using toggle switch on right side of instrument.
2. Ensure system has adequate sheath fluid in storage container and waste lines drain into a waste
location which is not full. Make sure sheath fluid container is at or below instrument level.
3. Click the xPONENT shortcut to start the software. Login to software using username and
password.
4. Click Eject on the System Status bar to eject the XY platform.

16 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

5. Add a 96 or 384 plate to the XY platform. For a filter plate a add large, flat disk to make sure
the probe does not puncture the well bottom. Otherwise no probe height adjustment tools are
needed.
6. From the Home page click Probe and Heater. On the Probe and Heater page, select the Plate
Type (96 well or 384 well) and enter a plate name.
7. Select the appropriate wells to calibrate probe height. This could be within the plate, the reservoir,
or the strip well locations in the off-plate reagent area. Make sure NO LIQUID is in the wells.
8. Click Auto Adjust Height. The probe will check heights in each well designated.

Luminex®  | 17
 9. Click Save to save the plate settings. The plate name will appear when building assay protocols.
10. Readjust the probe height whenever using new plate types, after system maintenance, or when
troubleshooting an acquisition problem. Probe Height is the MOST COMMON error encounted.
Daily System Start-up
1. On the Home page, click System Initialization. This opens the Auto Maint tab on the Maintenance
page.
2. On the Admin, System Setup tab there are three options listed below that can be used for system
initialization. Calibration is required weekly , however Performance Verification should be done
daily to check system integrity and insure calibration is valid.
a. Warm-up, washes, calibration and performance verification

b. Warm-up, washes, performance verification

c. Warm-up, washes
These instructions demonstrate option “a” above.
3. Make sure the Calibration Kit and Performance Verification Kit information has been imported into
the software using the CDs provided with the kits.
4. Select the correct kit lot numbers for your Calibration and Verification kit using pull down menus.
5. Eject the XY tray using the System Status bar.
6. Add two strip wells into the off-plate reagent area.
7. Notice the plate layout in the software which directs color coded reagent locations
18 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide
8. Add water and 70% isopropanol or 70% ethanol to the correct reservoirs within the Off Plate
Reagent area.
9. Vortex all Calibration and Performance Verification Kit reagents for ten seconds each.
10. Using the color coded plate layout diagram in the Reagents area of the tab as a guide, add F3DCAL1,
F3DeCAL1, F3DCAL2 , and F3DCAL3 to positions SA1, SB1, SC1, and SD1 on the first well strip.
11. Add F3DVER1, F3DeVER1, F3DVER2, Fluidics 1, and Fluidics2 to SA2, SB2, SC2, SD2, and SE2
on the second well strip as shown in the plate layout.
12. Click the Run button. The run should take 10 to 45 minutes depending on which system initialization
option is selected and if the system is
already warmed up.
13. Once complete click Report to view the
Calibration and enhanced Performance
Verification report.
Notes:
• You can import individual CAL and VER
information from the Lot Management tab.
• You can run individual reagents on the
Cmds & Routines tab; however, the
Performance Verification reports will not be
generated. You can only run Fluidics as part
of the Performance Verification Routine.
• You can create custom routines on the
Cmds & Routines tab; however, they will
not generate the Performance Verification report in this manner.
• Causes for failing calibration or verification may be that the reagents are not vortexed, reagents in
wrong well locations, or selecting wrong kit lot values.

System Shutdown
Perform the System Shutdown routine every day after running the system. This routine sanitizes the
fluidics lines, removes residual buffer, and prevents clogs from forming in the sample probe.
1. On the Home Page, click Shutdown.
2. Add 20% bleach solution and water to the reservoirs designated on the plate layout.

Luminex®  | 19
 3. Click Run.
Other Suggested Maintenance:
Any time you experience acquisition problems (or once a week if running debris ridden samples) we
suggest the following:
1. Remove the sample probe following instructions that can be found in the FLEXMAP 3D User’s
manual and place it in a sonicator bath for five minutes.
2. Rinse the probe with water.
3. Replace and readjust the probe height.
4. Run the Weekly Maintenance routine on the Maintenance page, Cmds & Routines tab.
5. Calibrate the system and run Performance Verification.

You can run individual commands and routines from Cmds & Routines tab. When running
calibration or performance verification reagents be sure that the selected lot number is the current
active lot on the Lot Management tab.

20 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

Collecting Data Without a Previously Created Protocol:
1. On the Home page click Create a New Batch from a new Protocol.
2. On the Settings tab click:
• Name
• Bead Type (MagPlex or MicroPlex)
• Volume to aspirate (20 to 200 ul)
• DD gate (usually 7500 to 15000)
• Reporter Gain (Standard or Enhanced PMT)
• Analysis Type (Quantitative, Qualitative, Allele Call, or none)
• Enter number of Standards and Controls as needed
3. Click Next

Luminex®  | 21
 4. On the Analytes tab enter:
• Analyte (Bead/Region number)
• Names for each analyte (notice by right clicking you can paste a list)
• Default Analysis Changes (weighting, type of curve fit)
• Click Next
Notice that by right clicking you are given the option of pasting data into the software

5. The Stds & Ctrls tab will only require information if the assay analysis type requires standards or
controls.
• To use standards and controls already in the system, click Apply Std/Ctrl Kit.
• To create a new kit, enter kit information at the top of the screen.
• For each standard or control, enter information in the tables. Notice you can right click to

22 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

 past values in from a text or spreadsheet.
• Under Admin page, Batch Settings tab you can set up kits so that entry of standard specific
information (name, lot #, exp date) is optional.
• To set up a dilution series, enter the value of the highest standard in the analyte column
and select one of the preset Dilution options (1:2, 1:5 or ½ log). You can also type your
own dilution factor in the box (1:10, 1:100, or 1:0.33 for a lowest to highest dilution curve).
Standards can also be resorted and dilution curves applied as needed.
• Click Next.
6. The Plate Layout tab helps you set up where samples will be placed on the plate.
• To add well commands, highlight the appropriate wells and mark them as Unknown, Standard,
Control, Background, or Wash. You can also delete commands that you’ve added, and
change the starting location on the plate. If you wish to run in replicate, change the Replicate

Luminex®  | 23
 Count to the appropriate number and the grouping to your preferred grouping method.
• To add maintenance commands, choose the desired command from the drop-down list.
Highlight the well that you want to apply it to, and then choose Before Well Command or After
Well Command. If you are working with more than one plate, choose the Add & Change Plate
page. Here, you can add a plate, change the order of the plates, and scroll through all plates.
• As you add commands to your plate, they appear in the command sequence chart. Here, you
can give each of your wells an ID. You can also import an ID list and move your commands
up and down in the command sequence chart.
a. Click Save to save the batch and run it later.
b. Click Save Pcol to save the protocol and standard and control information to use
later for another batch.

24 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

 c. Click Run Batch to begin data acquisition.
Collecting Data Using an Imported or Previously Created Protocol:
1. On the Home page, highlight the protocol.
2. Click Create a batch using a previously created protocol.
3. Enter a batch name and confirm the selected protocol.
4. Click Next to open the Std &Ctrl tab. Confirm the standard and control lots selected or choose another.
5. Click Next to open the Plate Layout tab. Add or remove samples as desired.
6. Eject the XY platform and insert the plate. Be sure you have adjusted the probe height for that
particular plate.
7. Click Run Batch.
Notice you can also Save this batch as pending and run at a later time or you can extract the protocol
for use later with other batches by clicking Save Protocol. This is most useful if you changed
protocol defaults during batch setup.

Luminex®  | 25
 Data Analysis
1. Open the Results page, then open the Saved Batches tab
2. Select the batch of interest by double clicking.
3. Select from Statistic to look at all analyte data at once or choose Analyte to look at individual
analyte data.

26 |  xPONENT 4.0 and FLEXMAP 3D User’s Guide

Generating Reports
Reports can be generated for collected batch data, calibration results, performance verification
results and system status. To generate a report:
1. On the Results Page, click the Reports tab.
2. Select the report. Batch, Calibration Summary, Performance Verification, Protocol or System Log
reports are available.
3. Select the type and analytes if needed.
4. Click Generate.

Luminex®  | 27
UNITED STATES
Luminex Corporation
12212 Technology Blvd..
Austin, Texas 78727
United States
Tel: 512.219.8020
Fax: 512.219.5195
CANADA EUROPE www.luminexcorp.com
Luminex Molecular Diagnostics Luminex B.V. info@luminexcorp.com
439 University Ave, Suite 900 Krombraak 15
Toronto, ON M5G 1Y8 4906 CR Oosterhout
Canada The Netherlands
Tel: 416.593.4323 Tel: +31.162.408333
Fax: 416.593.1066 Fax: +31.162.408337

©2009 Luminex Corporation and xMAP are trademarks of Luminex Corporation. Specifications and features subject to change without notice.

The FLEXMAP 3D is a class 1(I) laser product. 89-30000-00-180