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  • Asma Saparas (SP19-BSI-007) .Lab7

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    asad shafiq
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    The document summarizes a lab activity where the student: 1. Searched the UniProtKB database and found that it contains 263,798 records for hemagglutinin. Only 0.329% of these entries have been manually curated. 2. Learned that the accession number for hemagglutinin from Influenza C virus isolated in 1966 is P07975, and that its hosts are humans and pigs. 3. Identified the hemagglutinin protein's molecular function as host cell surface receptor binding and biological process as endocytosis involved in viral entry into host cell.

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    The document summarizes a lab activity where the student: 1. Searched the UniProtKB database and found that it contains 263,798 records for hemagglutinin. Only 0.329% of these entries have been manually curated. 2. Learned that the accession number for hemagglutinin from Influenza C virus isolated in 1966 is P07975, and that its hosts are humans and pigs. 3. Identified the hemagglutinin protein's molecular function as host cell surface receptor binding and biological process as endocytosis involved in viral entry into host cell.

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    11 views2 pages

    Asma Saparas (SP19-BSI-007) .Lab7

    Uploaded by

    asad shafiq
    The document summarizes a lab activity where the student: 1. Searched the UniProtKB database and found that it contains 263,798 records for hemagglutinin. Only 0.329% of these entries have been manually curated. 2. Learned that the accession number for hemagglutinin from Influenza C virus isolated in 1966 is P07975, and that its hosts are humans and pigs. 3. Identified the hemagglutinin protein's molecular function as host cell surface receptor binding and biological process as endocytosis involved in viral entry into host cell.

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    © All Rights Reserved
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    of 2

    Lab Activity 7

    Asma Saparas (SP19-BSI-007)

    Working with Databases

    Activity I: Go to expasy.org

    Activity II: Perform the search by selecting UniProtKB database. Answer the following
    questions.

    1. How many records correspond to hemagglutinin in UniprotKB database?


    263,798 

    2. What percentage of hemagglutinin entries are manually curated and verified?


    Reviewed data 868/263798x100=0.329

    3. What is the accession number of hemagglutinin protein isolated from Influenza C virus in
    1966?
    P07975
    4. What are the hosts of Influenza C virus? Is it possible that this virus has jumped hosts
    sometime in the evolution?

    Yes, It’s possible, there are 2 hosts.


    Homo sapiens (Human) [TaxID: 9606]
    Sus scrofa (Pig) [TaxID: 9823]

    5. Name 1 molecular function and 1 biological process that have been annotated against your
    protein of interest by the GO database?

    GO - Molecular function:

     Host cell surface receptor binding

    GO - Biological process

     Endocytosis involved in viral entry into host cell

    6. Is this protein a part of membrane?

    Yes it is a part of membrane.


    7. What mutation would lead to loss of 96% of the esterase activity and at what region?

    At 71 Position

    8. How many helices and beta sheets do you see in the secondary structure?

    32 Beta Strands
    13 helices

    9. What is the weight in Da of the protein?

    Mass (Da):72,131

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