Results

Table 1: Concentration of different elements in the Prepared multi-elements standards

Elements: Concentration of the elements in the multi element standard /ppm:

Standard Standard Standard Standard Standard Standard
1: 2: 3: 4: 5: 6:
Cu 0.05 0.2 0.4 0.8 1 2
Fe 0.25 1.0 2.0 4.0 5.0 10
Mg 1.25 5.0 10 20 25 50
Mn 0.5 2.0 4.0 8.0 10.0 20

Sample Calculations for the prepared standards:

For (Cu)

From 1000 ppm stock solution

Cu:

C1V1 = C2V2

(1000 ppm) (V1) = (10 ppm) (100mL)

V1 = 1 mL

Table 2: Concentrations of the elements in the multi-elements working standard and

volumes of the 1000ppm standards to prepare the multi-element working standards.

Elements: Concentration of the Volume of 1000ppm standards to

elements in the multi- prepare themulti-element working
element working standard/ mL:
standard/ppm:
Cu 10 1
Fe 50 5
Mg 250 25
Mn 100 10

Preparation of the standards from the multi-element.

Sample calculation of Cu and Fe:

Cu standard 1
 C1V1 = C2V2
10ppm V1 = (0.05ppm) (100mL)

V1 = 0.5ml of working solution

Fe standard 2

10ppm V1 = (0.2ppm) (100mL)

V1 = 2 mL of working solution

Table 3: Calculated volumes withdrawn from working solution to make standards.

Standard Multi-element working volume used / mL:

number:
1 0.5
2 2
3 4
4 8
5 10
6 20

Table 4: prepared standards concentra on of Cu and emission intensity.

Cu concentration/ppm: Emission intensity/au:

0.05 13115.86807
0.2 46271.89821
0.4 93412.51172
0.8 183848.58
1 233556.7994
2 453319.9545
Blank 4474.277642
 500000
450000 y = 226364x + 2701
R² = 0,9998
400000
Emmision intensity/au

350000
300000
250000
200000
150000
100000
50000
0
0 0,5 1 1,5 2 2,5
Concentration/ppm

Figure 1: Calibration curve for Cu standards Emission intensity/au plotted against

concentration/ppm.4

Table 5: prepared standards concentration of Fe and emission intensity.

Fe concentration/ppm: Emission intensity/au:

Blank 57.45562273
0.25 6458.532451
1 42754.03168
2 90520.66421
4 178900.3995
5 224947.0708
10 432946.8093

500000
450000 y = 43648x + 893,01
Emission intensity/au

400000 R² = 0,9992
350000
300000
250000
200000
150000
100000
50000
0
0 2 4 6 8 10 12
Concentration of Fe/ppm

Figure 2: Calibration curve for Fe standards Emission intensity/au plotted as a functionof

concentration/ppm.
Table 6: prepared standards concentra on of Mg and emission intensity.

Mg Concentration/ppm: Emission intensity/au:

Blank 745.6894589
1.25 673428.5106
5 2547744.1
10 4872916.735
20 9043751.999
25 11164872.19
50 19091099.74

25000000

20000000 y = 376448x + 918997

R² = 0,9908

15000000

10000000

5000000

0
0 10 20 30 40 50 60
Concentration of Mg/ppm

Figure 3: Calibration curve for Mg standards Emission intensity/au plotted against concentration/ppm.

Table 7: prepared standards concentra on of Mn and emission intensity.

Mn Emission intensity/au:
concentration/ppm:
blank -78.66814183
0.5 64078.81199
2 248783.146
4 493707.3481
8 963410.1253
10 1222303.45
20 2359601.714
 2500000
y = 117752x + 18650
R² = 0,9997
2000000
Emmision intensity/au

1500000

1000000

500000

0
0 5 10 15 20 25
Concentatin of Mn/ppm

Figure 4: Calibration curve for Mn standards Emission intensity/au plotted as afunction of

concentration/ppm.

Table 8: Mass of samples of tea samples

Sample: Mass of a sample /g

1 2
Five roses 0,3009 0.3009
Green tea 0.3010 0,3012
Rooibos 0,3043 0,3017

Table 9: Samples of teas with Emission intensity/au for Cu

Sample: Emission intensity / au:

Rooibos 1 15503.15226
Rooibos 2 13171.27809
Green tea 1 14327.94232
Green tea 2 12370.45084
Five roses 1 24905.03877
Five roses 2 17988.9859
 Table 10: Samples of teas with Emission intensity/au for Fe

Sample: Emission intensity / au:

Rooibos 1 67684.96231
Rooibos 2 2359601.714
Green tea 1 52189.0731
Green tea 2 43295.53153
Five roses 1 51431.61888
Five roses 2 47158.81343

Table 11: Samples of teas with Emission intensity/au for Mg

Sample: Emission intensity / au:

Rooibos 1 3764511.607
Rooibos 2 3651952.511
Green tea 1 4320508.397
Green tea 2 4358001.428
Five roses 1 5232521.791
Five roses 2 5417508.355

Table 12: Samples of teas with Emission intensity/au for Mn

Sample: Emission intensity / au:

Rooibos 1 52613.64133
Rooibos 2 51014.98066
Green tea 1 928895.1073
Green tea 2 1027848.386
Five roses 1 531955.7759
Five roses 2 527538.562
 Sample calculation:
From figure 1:

the calibration curve of different Cu concentrations against emission intensity. Calculation

forRooibos 1:
y = 226364x + 2701

67684.96231= 226364x +2701

x = 0.287
m= 0.287077284 mg/L x 0.05 L

= 0.01435mg × 1000

= 14.35 µg

= 14.35 µg/ 0.3043 g

= 47.17 µg/g

The calculations for the masses of elements for all the tea samples were done using the same
method as the above. The mass values are shown bellow.

Table 13: Masses calculated from the calibration curves (µg/g)

Name of tea: Copper Iron Magnesium Manganese

(µg/g): (µg/g): (µg/g): (µg/g):
1 2 1 2 1 2 1 2
Rooibos 47.14 97.45 119.80 170.22 1038.20 2036.02 65.73 66.75
Green tea 51.14 21.92 183.28 136.99 2079.05 1664.44 1639.46 1729.46
Five roses 27.45 39.08 147.40 174.77 1507.75 1104.53 573.43 559.26