Btbce Uct

M Tech (BCE&BT) CBCS Scheme and Syllabus Approved in
Academic Council meeting held on 17/04/2017
M.TECH (BIOCHEMICAL ENGINEERING &

BIO TECHNOLOGY)
SCHEME OF INSTRUCTION AND
EXAMINATION&SYLLABUS
UNDER
CHOICE BASED CREDIT SYSTEM (CBCS)
FOR THE BATCH ADMITTED IN 2016-17
SCHEME OF INSTRUCTION AND EXAMINATION – (CBCS)

FOR
M. TECH– BIO CHEMICAL ENGINEERING& BIO TECHNOLOGY
(With effective from 2016-2017)
FIRST YEAR FIRST SEMESTER
SYLLABUS Catego PERIODS / WEEK MARKS Duration of Credits

REF. NO COURSE ry (60 MIN) exam(hrs)
Theory Pra Tutorials Sess. Exam
ctic
al
Molecular Core-1 3 - - 25 75 3 3
MBTT-101 Biology &
Genetic Engg.
MBTT-102 Cell Culture Core-2 3 - - 25 75 3 3
Techniques in
Bio Technology
Bio Reactor Core-3 3 - - 25 75 3 3
MBTT-103 Design &
Process
Engineering
MBTT-104 Elective –I PE-1 3 - - 25 75 3 3
MBTT-105 Elective-II DE-1 3
3 - - 25 75 3
Elective-III OE-1 3 - - 25 75 3 3
MGENT-101
MBTP-101 Molecular Core - 3 1 25 50 4 2
Biology &
Genetic Engg.
Lab
MBTP-102 Scientific Core 4 - 75 Report 2
Writing/ Evaluation
Mini Project/ &
Instrumental Oral
analysis lab/ Presentation
Seminar -I
18 07 01 250 500 22
Elective –I Elective-II Elective-III

Program Electives -1 (PE-1) Department Electives- 1 Open Electives- I (OE-1)
(Discipline specific) (DE-I) General across departments
Interdisciplinary
1.Industrial Microbiology 1.Modern analytical tools & 1 Statistical Analysis and Design
2.Science & Technology of techniques of Experiments
Biomaterials 2.Project Engg& Management 2. Operations Research for
++++++3.Novel Reactors 3.Virtual instrumentation Engineers
4. Solid Waste Treatment & 3 Food BioTechnology
Management
5 Bio Nano Technology
FIRST YEAR SECOND SEMESTER

SYLLABUS Catego PERIODS / WEEK MARKS Duration of Credits
REF. NO COURSE ry (60 MIN) exam(hrs)
Theory Pra Tutorials Sess. Exam
ctic
al
Computational Core-4 3 - - 25 75 3 3
MBT T-201 Techniques in
Bio Technology
MBT T-202 Protein Core-5 3 - - 25 75 3 3
Engineering &
Enzyme
Technology
Down Stream Core-6 3 - - 25 75 3 3
MBT T-203 Processing in
Biotechnology
MBT T-204 Elective-IV PE-II 3 - - 25 75 3 3
MBT T-205 Elective -V DE-II 3
3 - - 25 75 3
Elective -VI OE-II 3 - - 25 75 3 3
MGENT-201
MBT P-201 Down Stream Core - 3 1 25 50 4 2
Processing in
Biotechnology
Lab
MBT P-202 Seminar-II Core 4 - 75 Report 2
Evaluation
&
Oral
Presentation
18 07 01 250 500 22
Elective –IV Elective-V Elective-VI

Program Electives -2 (PE-2) Department Electives- 2 Open Electives- 2 (OE-2)
(Discipline specific) (DE-2) General:across departments
Interdisciplinary
1.Pharmaceutical biotechnology 1.Environmental Biotechnology 1.Patents & Intellectual property
2. Advanced Oxidation Processes & 2.Process Flow sheeting rights
Technology 3.Environmental Impact & 2.Industrial Psychology
3. Electrochemical Engineering Assessment 3. Fuel Cell Technology
4. Energy Bio Technology
SCHEME OF INSTRUCTION AND EXAMINATION – (CBCS)

FOR
M. TECH– BIO CHEMICAL ENGINEERING & BIO TECHNOLOGY
(With effective from 2016-2017)
SECOND YEAR FIRST SEMESTER
Code Course Category Periods/day Assessment Marks Credits
Library Practica Internal Ext Interna Ext.

l l
MBTP301 Core
Dissert Midterm 8
- 100 -
ation evaluation
(Part – 4 4 of Report
I) presentation - 50 4
150 - 12
SECOND YEAR SECOND SEMESTER
Code Course Category Periods/day Assessment Marks Credits
Library Practical Internal Ext Interna Ext.

l
MBTP401 Dissert Core Pre- 100 - 8
ation submission
(Part – 4 4 Viva-voce
II) Report - 100 8
Evaluation
Final - 50 4
Viva-voce
100 150 20

BIO TECHNOLOGY)
CBCS SYLLABUS
I YEAR – I SEM
CORE COURSES
Core-1 Molecular Biology and Genetic

Engineering
Core-2 Cell Culture Techniques in Bio
Technology
Core-3 Bio Reactor Design & Process
Engineering
MOLECULAR BIOLOGY AND GENETIC ENGINEERING

Credits : 3
MBTT : 101 Periods/ Week : 3
Sessionals : 25 Duration of the Exam : 3 Hours
Examination : 75 Nature of the Exam : Theory
Course objectives
• To understand the concept of molecular biology, Dna replication, transcription and

translation.
• To learn about plasmids and modifying enzymes
• To learn blotting techniques, dna sequencing
• To learn the concept of Genetic engineering, rdna technology.
Unit-I
Introduction To Molecular Biology
Molecular biology, Basic concepts in Molecular Biology, Classification, Isolation,

Characterization, chemical structure and functions of biomolecules, Nucleic acids and their role
in gene constitution, Anatomy of the genome, Structure, Organization of the genome, in their
nucleus, mitochondria and chloroplasts, Replication of DNA, Semi-Conservative replication,
Messelson Stahl’s experiment, Replication of circular and linear DNA, DNA damage and
repair- Photo reactivation ,excision repair, mismatch repair, post replication SOS repair Genetic
Code, Genetic evidence for the nature of the code, Elucidation of the code, Initiation and
termination codons, Properties of genetic code, Transcription of DNA, Initiation, Elongation and
Termination, RNA Polymerases, Structure Of m-RNA, t-RNA, r-RNA, Translation of RNA,
Ribosomes, polypeptide chain initiation, elongation and termination
Unit -II
General properties and classification of plasmids, Significance of Plasmids, Host controlled

restriction mapping, Restriction endo nucleases, Types and classification, Modifying enzymes
used in molecular cloning-Methylases, Polymerases, Ligases, Phosphatase and nucleases.
Plasmid and Lamda Phage vectors, Cosmidvectors, Yeast and Bacculo Virus vectors.
Unit -III
Strategies for genomic and c-DNA library construction, Amplification of DNA by Polymerase
chain reaction, Labelling of nucleic acids and immunological Probes, Selection of Recombinant
clones, Blotting techniques(Southern, Northern, Western, Zoo Blots),Colony Hybridization and
Library Screening, Chrosome walking and jumping, Mapping of restriction sites Methods of
DNA sequencing(Maxam Gilbert, Snagers and Automated),Screening of the cloned
genes(Hybrid arrest and hybrid release translation),Site directed Mutagenesis, Antisense and
RNAi technology
Unit- IV
Introduction To Genetic Engineering
Basic concepts of genetic engineering, Overview of recombinant DNA technology-concept,

basic methodology, purpose, Methods and Techniques of gene transfer, Selection, Identification
and recovery of transformed cells.
Unit -V
Gene expression, Genetic Stability, Molecular Marker Development and their utility in
genotyping, DNA Fingerprinting Technique, AFLP,RFLP,RAPD,SSR.
• COURSE OUTCOMES(Graduate to have)

• An ability to isolate DNA to perform blotting techniques
• An ability to perform DNA sequencing
• An ability to perform genetic engineering techniques.
• An ability to have keen knowledge in molecular biology
Examination: Question paper consists 8 Questions selecting at least one from each unit and 5 to
be answered out of 8
Text Books:
1.MolecularCloning,Smabrook et al,Cold Spring Harbour.
2.Essentials Of Molecular Biology,Malacinski and Freifelder Jones and BartleLt publishers.

3.Genomes,T.A.Brown,John W
iley and Sons PTE Ltd.
4.Cell and Molecular biology, Concepts and experiments Gerald Karp John Wiley and Sons.
5.The Cell-A Molecular Approach, GM Cooper Asm Press.
6.”Principles of Gene Manipulation” by R.W.Old and S.B. Primrose 3rd Edition, Blackwell
Scientific Publication 1985.
7.”GENES VI” By B.Lewin.
8.”From Genes To Clones” by E.L.Winnecker.
9.”Genes VII” By Benjamin Lewin,Oxford.
CELL CULTURE TECHNIQUES IN BIO TECHNOLOGY

Credits : 3
MBTT : 102 Periods/ Week :3
Examination : 75
Course objectives
• To understand structure of prokaryotic and eukaryotic organisms.

• To understand general tissue culture techniques.
• To study different plant cell cultures.
• To study animal cell culture, gene cloning techniques.
Unit-I
Cell Doctrine: Structure of Eukaryotic and Prokaryotic cells, Intracellular Organelle and their
functions, Comparison between plant and animal cells, Cell-Junctions, Cell-Cell Adhesion and
Extracellular matrix of animal and plant cells.
history, Laboratory requirements and General techniques, Introduction, Requirements,

Techniques, Tissue Culture Media: Introduction, Media Constituents, Media Selection, Media
Preparation.
Unit -II
Plant Cell Culture: Plant Tissue Culture media preparation and sterilization, The concept of
totipotency, Initiation of Callus and Suspension cultures, The Technique of subculture, Single
Cell Cultures, Role of Plant Growth regulators during differentiation, Micro propagation of
Ornamentals, Forest Trees and Horticultural Plants, Organogenesis and Somatic embryogenesis,
Encapsulation and production of Artificial Seeds, Embryo culture and Embryo Rescue. Anther
and Pollen Culture, Production of haploid plants, Somaclonal Variation and Crop Improvement.
Cryobiology of Plant cells and tissues in culture, Technology of Freeze preservation.
Unit -III
Secondary plant products useful to mankind, Physical and chemical factors that influence the
production of secondary metabolites, Invitro Induction of Hairy root cultures, Major biosynthetic
pathways leading to the production of medicinally important compounds, Manipulation of
genome for metabolic pathways, Mass cultivation of plant cells in different types of bioreactors,
Novel reactor designing concepts, Permeabilization, Immobilization and Elicitation of plant cells
for the enhanced production of secondary products, Biotransformation using cell culture
methods.
Unit -IV
Animal Cell Culture: Introduction, Scope, balanced Salt Solutions and Simple Growth Media,
Serum and it’s quality, medium sterilization. Basic techniques of animal cell culture and their
application. Animal Cell metabolism, Regulation and nutritional requirement, Animal cell
growth, Characteristics and Kinetics, Nutrient, Substrate and product transport through
mammalian cells, Micro carrier attached growth, Cell culture in continuous, perfusion and
hollow fibre-reactor, mass transfer in mammalian cell culture, Scale up of cell-culture processes.
Unit -V
Gene Cloning techniques for mammalian cells, Cloning in mammalian cells, Expression of
mammalian genes in prokaryotes and eukaryotic systems, Transgenic animals. Preservation and
maintenance of animal cell lines, cryopreservation and transport of animal germplasm(ie semen,
ovum and embryos).

• An ability to have knowledge of prokaryotic and eukaryotic organisms.
• An ability to perform any tissue culture techniques.
• An ability to produce medicinally useful secondary metabolites.
• An ability to grow animal cell under tissue culture conditions.
Examination: Question paper consists 8 Questions selecting atleast one from each unit and 5 to
TEXTBOOKS:
1. Molecular Biotechnology: Primrose.

2. Experiments in plant tissue culture by John H.Dodds and LorinW.Robert.
3. Plant Tissue Culture: Theory and Practice by S.S.Bojwani and M.K.Razdan(1996) Elsevier,
Amsterdam
4. Animal Cell Biotechnology: R.Espier and J.B.Griffiths(1988) ,Academic press.
5. Living resources for biotechnology, Animal cells: A.Doyle,R.Hay and B.E.kirsop, University
Press, Cambridge
BIO REACTOR DESIGN & PROCESS ENGINEERING

Credits : 3
MBTT : 103 Periods/ Week : 3
Course objectives
• To learn about different types of bioreactors.

• To understand the concept of fermenter.
• To learn operating issues that effect bioreactor.
• To learn the concept of ideal and non ideal reactor.
Unit-I
Product Formation in Microbial Cultures: Growth associated and non-growth associated

Kinetics, Energy requirements, Electron transfer concept, maintenance energy, magnitude and
control of maintenance energy, Effect of maintenance. Effect of inhibition and activation of
growth, competitive and non-competitive inhibition, Product and substrate inhibition, activators
effect on batch and continuous system.
Definition of Bioreactor, types of reactors-batch, plug flow reactor(PFR),continuous stirred tank

reactors(CSTR),Fluidized bed reactor bubble column, air lift fermentor, etc.
Unit-II
Stirred Tank batch bioreactor: For all enzymes, cell cultures.
Continuous Stirred Tank Bioreactor-Operation of mixed reactor-enzyme reactions in mixed

reactor, performance equation for M-M kinetics, substrate and product inhibition kinetics,
chemostat with cell cultures-steady state cell and substrate concentrations and productivity as a
function of dilution rate.
Plug Flow and Packed bed Bioreactor-Performance equation with Michelin-Menten kinetics,
substrate inhibition and product inhibition, plug flow reactor for immobilized enzymes, operation
of plug flow reactor in constant feed rates policies.
Fed Batch reactor: Applications of fed reactor, fed batch operation of mixed reactor, material
balance on cell and substrate.
Unit-III
Basic function of a fermenter for microbial or animal or animal cell culture, basic bioreactor
design criteria, overview of bioreactor types-stirred tank bioreactor, bubble column bioreactor,
air lift reactor, propeller loop reactor, jet loop reactor, schematic overview of fermenter with
control system, operating issues that effect reactor design, aeration and oxygen mass transfer in
bioreactor system, design of chemo stat.
Unit-IV
Analysis of Non-ideal Reactor Analysis:Concept of ideal and non-ideal reactor, residence time
distribution, models of non-ideal reactors, plug flow reactor for microbial processes, Mass
transfer in biochemical processes.
Unit -V
Multiphase Bioreactors-packed bed with immobilized enzymes or microbial cells.
Unconventional Bioreactors: Hollow fibre reactor, membrane reactor, perfusion reactor for
animal and plant cell cultures

• An ability to have knowledge about bioreactors.
• An ability to perform and operate stirred tank batch bioreactor.
• An ability to handle the reactor design, aeration, oxygen, mass transfer in bactors
• An ability to handle immobilized enzymes or microbial cells in bioreactors.
Examination: Question paper consists 8 Questions selecting at least one from each unit and 5
to be answered out of 8
Text Books:
1.Blanch H.W. and DoughlasS.Clark,BiochemicalEngineering,CRC press,1997.
2.Micheal L Shuler and Fikretkargi,BioprocessEngineering,Prentice-Hall of India Pvt Pld,2008.
3.StanburyP.F.Whitaker, Principles of fermentation technology,Elseiver India Pvt,Ltd,2007.
4.ArthurT.Johnson,Biological Process Engineering,AnAnological Approach to fluid

flow,Heattransfer,John Wiley and sons,1998.
5.PaulineM.Doran, Bioprocess Engineering Principles.Academic Press,1995.
6.D.G.Rao Introduction to Biochemical engineering Tata Mc Graw-Hill,2005.

ELECTIVE-I
PROGRAM ELECTIVES
1 Industrial Microbiology
2 Science & Technology of Biomaterials
3 Novel Reactors
ELECTIVE – I
INDUSTRIAL MICROBIOLOGY
Credits : 3
MBTT : 104(1) Periods/ Week :3
• Course objectives
• To learn scope and relevance of microbiology in biotech industry.
• To learn isolation and preservation of industrial microorganisms.
• To learn taxanomical classification and special characteristics of important industrial micro
organisms.
• To learn the process of producing primary and secondary metabolites.
Unit-I
History, scope and relevance of microbiology in biotech industry, Screening and Selection of
Microorganisms, Stock cultures, preparation of growth media, growth characteristics of
microorganisms. Transport of nutrients across cell membrane, Energy transduction mechanisms
in microbial cell fermentation.
Unit-II
Isolation, development and preservation of industrial microorganisms, Substrates for industrial
microbial processes, carbon and nitrogen substrates in production of media, Characteristics of
Production Media, Importance of production media, Optimization of fermentation media,
Microbial inoculum/starter culture development for fermentation processes.
Unit-III
Regulatory mechanisms of metabolic pathways in industrial strains,primary and secondary
metabolism, engineering analysis of metabolic pathways. Taxonomical classification and special
characteristics of important industrial microorganisms.
Unit-IV
Analysis of various microbial industrial processes used in the production of biomass, primary
and secondary metabolites, Production of Antibiotics(Penicillin and streptomycin), organic
acids(Acetic acid, Citric acid and Gluconic acid)
Unit - V
Alcoholicbeverages(Methanol and Ethyl Alcohol ), Fermented foods, Fuels, bio-fertilizers, bio
pesticides, bioleaching for metallurgy and effluent treatment and other industrial applications.

• An ability to prepare growth media for microorganisms.
• An ability to classify special important industrial microorganisms.
• An ability to produce antibiotics, organic acids.
• An ability to prepare fermented foods, biofertilizers.
Textbooks:
1.”Industrial Microbiology” by L.E.CasidaJr.Wiley Eastern Ltd.
2.”Comprehensive treatise on Biotechnology” by H.J.Rehm and G.Reed,Verlag-
Chemie,Weinheim.
3.”Microbiology” by PelzarM.J.Chan E.C.S and Kreig N.R., tata McGraw-Hill
4.”Principles of Cell Energetics” Biotolseries,Butterworth-Heinemann.
ELECTIVE – I
SCIENCE & TECHNOLOGY OF BIOMATERIALS
Credits : 3
MBTT : 104(2) Periods/ Week : 3
• Course objective
• To learn molecular dynamics at surfaces , textile derived structures.
• To learn biophotonics, biosensors, nano particles.
• To learn about polymers, technology of biopolyster.
• To learn about bone,skin,tissue engineering replacements.
Unit - I :
Biomaterials and Surface Science:
General biomaterial properties; bulk and surface; molecular dynamics at surfaces;
Visualizing molecules on surfaces by Atomic Force Microscopy, Atom arrangements in
materials and properties; Synthesis of ceramics, Glasses and Sol-Gels for Biotechnology;
Combinatorial approaches to materials design; General issues in material fabrication
Textile-derived structures; Routes to controlled porosity foams; Patterned surfaces;
Unit - II :
Bionanotechnology:
Nanocomposites; Nanostructured materials and Nanoparticles/Nanocomposites; Thin films
and coatings, Biologically functional materials; Analysis tools for materials;
Biophotonics, Biosensors, Bioactuators, and glucose sensors.
Unit - III :
Biopolymer sciences and engineering:
Polymer introduction and natural polymers I (Alginate , chitin , collagen, silk, etc.); natural
polymers II (proteins, genetic engineering); condensation polymerization (polyesters,
polyamides, polyethers mechanisms, etc.); free radical polymerization (PMMA, kinetics,
mechanisms, etc.) ; ring opening polymerizations (PLA, PGA, PCA, etc.) & co-
polymerizations; fibres/hydrogels/scaffolds; degradation.
Technology of biopolyester, microbial polysaccharides, microbial cellulose, bioadhesive,
polyglutamic acids; emerging trends in biocomposites-biodiode, polymer coated blood,
polymer precursors; properties & characterizations; emerging improved functions- genetics
& molecular biology; products of animal & plant cell cultures.
:
Unit –IV
Biomaterials in health sciences:
Biomaterials for skin, nerve, fat, bone regeneration; Bone: basic anatomy; composite
approach; fracture models. Skin: visco-elastic behavior; wound healing; tissue engineering:
approaches and mechanics; soft tissue interactions: biocompatibility, biodegradation,
extracellular matrix; soft tissue polymer implants; cartilage: structure & function; biphasic
theory; triphasic theory; tissue engineering replacements; load bearing replacements: yield
& fracture; manufacturing issues;
Unit V
Biomolecular science and engineering:
Overview; blood material interactions; hemostatis and thrombosis; blood compatibility
&invitro models of testing; inflammation, wound healing, foreign body response; immune
response; ethics, regulations & future societal challenges; structure & properties of cardio-
vascular biomaterials; cardio-vascular implants.
COURSE OUTCOMES(Graduate to have)
• An ability to have knowledge on atomic force microscope

• An ability to perform general issues in material fabrication,tectile derived
structures.
• An ability to perform different types of polymerization
• An ability to have keen information on blood.
Examination: Question paper consists 8 Questions selecting at least one from each unit
and 5 to be answered out of 8
Text Books and References:

1. “Bioprocess engineering – Basic concepts” by Michael L. Shuler and FikretKargi, PHI,
New Delhi (2003)
2. Biomaterials science: An introduction to materials in medicine B.D. Ratner , A.S.
Hoffman, F.J. Schoen and J.E. Lemons (Eds.) 1996, Academic Press, San Diego
ELECTIVE – I
NOVEL REACTORS
Credits : 3
Course objective
• To learn about different types of reactors cstr, ,pfr.
• To learn action of centrifugal field ona thin film of liquid on a surface.
• To learn operation of spinning disc reactor.
• To learn about micro reactors.
Unit – I
Introduction to Novel Reactors:
The use of different types of reactors to overcome limitations imposed by conventional
configurations .
The limitations ofconventionalbatch reactors, CSTRs, PFRs and PBRs with respect to
heterogeneous systems.
The concept of using other fundamental phenomena such as the centrifugal field to overcome
limitations.
Unit – II Kinetic versus Mass Transfer Control in Reactors
Catalytic processes with the sequential steps associated with migration of reactants fromthe fluid
bulk to the catalyst surface, adsorption/reaction/desorption of species on/from the surface, and
migration of products from the catalyst surface into the fluidbulk Operating regime
Unit – III
High Gravity Environment (Higee)
The innovation of the Higee environment, The coordinate systemfor rotating systems, The
action of a centrifugal field on a thin filmof liquid on a surface.
The action of the centrifugal field on the flow of liquid through a porous medium that is
rotating.The impact erences of the speed of rotation on the thickness of fluid boundary layers
Benefits of the reducedresidence times arising fromthe use of a high gravity environment
Unit – IV
Configuration/OperationofSpinning Disk Reactor (SDR)
A schematic of the basic configuration. Channeling of entry/exit of the process/reacting fluid
to the reacto, when it passes through the reactor. The impact of speed ofrotation on residence
time, heat and mass transfer resistances/
Configuration/Operation of Rotating Packed Bed (RPB) Reactor

A schematic of the basic configuration.The moving of the fluid around the solid catalyst,
Effect of speed of rotation on change in iquid boundary layer
Unit V
Micro-reactors: Description and operating principles, Different types of Micro reactors, Heat
transfer applications, mass transfer Applications, mixing applications.
• An ability to use different types of reactors

• An ability to perform kinetic versus mass transfer control in reactors.
• An ability to have knowledge in impact of speed of rotation on residence time, heat, mass
transfer.
• An ability to describe different types of micro reactors.
Examination: Question paper consists 8 Questions selectingatleast one from each unit and 5 to
Text Books and References

1.David Reay, Colin Ramshaw, Adam Harvey, Process Intensification- Reengineering for
efficiency, sustainability and flexibility, Butterworth Heinemann, (Elsevier) Edition: 2nd , 2013
2. Stankiewicz, A. and Moulijn, (Eds.), Reengineering the Chemical Process Plants, Process
Intensification, marcel dekker 2003
3 KameliaBoodhooo, Adam Harvey (Editors) Process Intensification Technologies for Green

Chemistry : Engineering Solution to Sustainable Chemical Processing, Wiley 2013
4.Oxley, P.; Brechtelsbauer, C.; Ricard, F.; Lewis, N.; Ramshaw, C.; “Evaluation of
SpinningDisk Reactor Technology for the Manufacture of Pharmaceuticals,”Ind. Eng.
Chem. Res.,39, 2175-2182 (2000).
ELECTIVE-II
DEPARTMENTAL ELECTIVES
1 Modern analytical tools & techniques
2 Project Engg& Management
3 Virtual Instrumentation
4 Solid Waste Treatment & Management
5 Bio Nano Technology
ELECTIVE – II
MODERN ANALYTICAL TOOLS & TECHNIQUES
Credits : 3
Course objective
• To learn microscopy, SEM and REM .
• To learn analytical techniques,immune techniques,biosensors.
• To learn fermentation process, flow injection.
• To learn chromatographic techniques, spectroscopy.
Unit-I
Principles and applications of analytical Tools and techniques in Biotechnology.
Microscopy-Phase contrast, Confocal, SEM&REM microscopic principles in biology.
Unit -II
Analytical techniques in biochemical systems, electrophoretic techniques -10, 20 gels, Pulse field
Electrophoresis, Western Blotting, Gel Documentation.
Immunotechnniques,Immunoflouresence,Immunohistocompatibility,Localization of cells in
tissue immunoblotting, Biosensors-Concepts and their applications.
Unit-III
Fermentation process- Gas analysis for O2 and CO2.
Flow Injection Analysis,Computational data acquisition of bioprocesses.Online Bioprocess

instrumentation, Techniques for Biochemical Processes.
Unit-IV
Chromatographic Techniques & Tools- Column (Affinity & Ion-Exchange

Chromatography),Thin Layer and paper Chromatography, GC and HPLC techniques.
Unit -V
Spectroscopy ; UV-Visible, FT-IR, NMR & Mass Spectrometry in Identification of

Biomolecules.
• An ability to use microscopes and its types in biology

• An ability to perform analytical techniques in biochemical systems
• An ability to perform all types of chromatography techniques
• An ability to have immense knowledge in spectroscopy.
Text Books:
1.”Biochemical Engineering Fundamentals” 2nded. By J.E.Bailey and D F Ollis,McGraw-

Hill(1986).
2.”Instrumental methods of Chemical Analysis” by B.K.Sharma 23rdedn,GOELPub.House.
3.”Spectrometric identification of organic compounds”,Bassler& Silverstein.
4.”Identification of organic compounds by William Kemp,.ELBS Publications.
5.Principles of Instrumental Analysis,5 thedn.D A Skoog,F.James and

Holler,TimothyA.Nieman,Harcourt Brace college Publishers,Florida,US.
6.Handbook of Instrumental Techniques for Analytical Chemistry, by J.A.Howell,Prentice

Hall,(1997).
7.Fundamentals of Analytical Biochemistry-Skoog& West
8.Instrumental methods Of Analysis-Ewing.

ELECTIVE – II
PROJECT ENGINEERING & MANAGEMENT

Credits : 3
Course objective
• To learn the concept of project, types, scope, significance.
• To learn about steps involved in project planning.
• To learn project plant engineering and project climatology.
• To learn about project finance,material acquisition.
UNIT -I
Introduction to Projects and Project Engineering

Concept of a Project , types of projects, Meaning and concept of Project Engineering ,
significance and scope, frame work , relevance to multidisciplinary engineering , proposal of
new Project ,( Peak and Off-Peak Demand)Product Life Cycle, Project Life Cycle,(Mortality
Curve), Material Selection or types of flow , Project cost estimation (Interest formulas , present ,
future worth , Annual equivalent , rate of return methods , Make or Bye decision, Depreciation,
Value analysis) .
Structure and organization of Project Engineering department , Stages in project engineering
process, role of Project Engineer, Project Manager, Project leader(Types of Project Leadership),
Role of Team Engineering in Project and Project engineering
UNIT-II
Project Planning : Steps involved, planning techniques, techno-economics and evaluation ,
conceptual designing , controlling and Value engineering
Project Capacity Management: Introduction, Bottlenecks ,Capacity Forecasting and Capacity
Planning (use of BEA & LPP ) ,capacity and Breakdown costs, Project identification and
formulation , proposal of new Project report ,various stages in the new proposed projects,
,overall frame work of Projects, feasibility of proposed projects.
Project Production Dynamics: types (selection & Economical criterion in selection -Production
Vs Productivity, Role of NPC, Tools & Indices of productivity,)factors affecting production,
Economical size of the Project, factors governing size, small scale industries-reasons for survival
and optimum firm, Input –Output Analysis
UNIT-III
Project Plant Engineering & Project Climatology :
Location & Layout: Need,types, Tools used for best Location and Layout selection
Building : Need, factor governing, types, selection , type of floor Lighting: Need, Need, Sources
of Light, types of work and light. Ventilation: Need, types of Ventilation, Sources ,Provision in
Plant Building .Air conditioning: Need, Types of Air conditioning, Building Design and Air
conditioning Material Handling: MH Types, role of AGV’s, Ergonomics or Human
Engineering and its significance
UNIT – IV
Project Finance: planning for finances , role of financing agencies , procedures
Project Scheduling : Elements, methods , Project scheduling by Jhonson’s algorithm, concept of
parallel critical paths, probability estimation, scheduling for manufacturing
Material Acquisition : Type and nature of materials (ABC & Pareto analysis) , types of
Inventory and types of decisions, EOQ, Spare parts Management ,procurement and its Types,
Procurement process, Stores organization, Pricing methods, Waste, Scrap, Disposal and Samples
Management, Warehouse Management
UNIT-V
Project Projection , co-ordination and Control: Project direction , project communications,
progress and performance control
Project Interfaces: Types of interfaces, pre and post commissioning interface
Risk assessment and mitigation : Types of project risks and analysis.
Precommissioning and Commissioning :Organisation , activities, Role of commissioning
manager, evaluation tools .
Project Implementation: Performance indicators, performance of Project engineering ,reporting

• An ability to organize project
• An ability to work on project capacity management and production management.
• An ability to plan for project having good lighting, air conditioning, material handling.
• An ability to plan for finances, probability estimation, types of decisions.
Examination : Question paper consists 8 Questions selecting at least one from each unit and 5
Text books :
1.Project Engineering and Management : Subhendu Moulik, Author UK , 2012
2.Project engineering : Sanford I. Heisler, The Wiley Eastern Publishers , New York, 2010
3.Production& Operations Management : Aswathapa , Himalaya Publications, 2012
Reference Books
1..Project Management – Chowdary, Tata McGrahill , 2009
2..Project Management & Control – P.C.K .Rao., Sultan Chand & Co, New Delhi
ELECTIVE – II
VIRTUAL INSTRUMENTATION
Credits : 3
Course objective
• To learn historical perspective,block diagram of virtual instrument.
• To learn vi programming techniques
• To learn data acquisition on pc
• To learn instrument control, development of process database management systems.
UNIT-I
Virtual Instrumentation: Historical perspective, advantages, block diagram and architecture

of a virtual instrument, data-flow techniques, graphical programming in data flow,
comparison with conventional programming. Development of Virtual Instrument using GUI,
Real-time systems, Embedded Controller, OPC, HMI / SCADA software, Active X
programming.
UNIT-II
VI programming techniques: VIS and sub-VIS, loops and charts, arrays, clusters and graphs,
case and sequence structures, formula nodes, local and global variables, string and file I/O,
Instrument Drivers, Publishing measurement data in the web.
UNIT-III
Data acquisition basics: Introduction to data acquisition on PC, Sampling fundamentals,
Input/Output techniques and buses. ADC, DAC, Digital I/O, counters and timers, DMA,
Software and hardware installation, Calibration, Resolution, Data acquisition interface
requirements.
UIT-IV
VI Chassis requirements. Common Instrument Interfaces: Current loop, RS 232C/ RS485,
GPIB. Bus Interfaces: USB, PCMCIA, VXI, SCSI, PCI, PXI, Firewire. PXI system
controllers, Ethernet control of PXI. Networking basics for office & Industrial applications,
VISA and IVI.
UNIT-V
VI toolsets, Distributed I/O modules. Application of Virtual Instrumentation: Instrument
Control, Development of process database management system, Simulation of systems using

VI, Development of Control system, Industrial Communication, Image acquisition and
processing, Motion control.

• An ability to produce architecture of virtual instrument.
• An ability to draw loops, charts, arrays clustered and graphs.
• An ability to do networking basics for industrial applications.
• An ability to perform instrument control.
TEXT BOOKS
1. Gary Johnson, LabVIEW Graphical Programming, Second edition, McGraw
Hill, Newyork, 1997.
2. Lisa K. wells & Jeffrey Travis, Lab VIEW for everyone, Prentice Hall, New Jersey, 1997.
3.Kevin James, PC Interfacing and Data Acquisition: Techniques for Measurement,
Instrumentation and Control, Newnes, 2000.
WEB RESOURCES www.ni.com, www.ltrpub.com
ELECTIVE – II
SOLID WASTE TREATMENT AND MANAGEMENT
Credits : 3
Course objective
• To learn sources of solid wastes, storage and transportation.
• To learn physical, chemical, biological solid waste treatment.
• To learn handling of hazardous wastes
• To learn biomedical waste management.
UNIT-I
Introduction: Types and sources of solid wastes, Characteristics and collection, Solid waste
volume reduction, Storage and transportation
UNIT-II
Solid Waste Treatment System: Physical, Chemical and Biological treatment Systems, Reuse
and recycling of solid waste- Incineration, Composting and Composting Plants, Ultimate
disposal of solid waste Sanitary landfills planning and design, Methods and cost comparison
UNIT-III
Handling of hazardous wastes- Collection of hazardous wastes and care in handling quantities of
hazardous wastes generated, Storage of hazardous wastes, Transportation and Shipment of
hazardous wastes. Final disposal of hazardous wastes- Site selection, incineration, land filling,
Leachates, treatment and disposal
UNIT-IV
Biomedical waste management- Concepts, treatment, and legislations; Electronic Waste (e-
waste) management- Concepts, treatment, and legislations
UNIT-V
Case studies based on course content and related to process industries

• An ability to perform incineration, composting, landfills planning.
• An ability to collect hazardous wastes and perform waste treatment.
• An ability to manage biomedical waste.
• An ability to perform ultimate disposal of solid waste
Text Books
1. Integrated Solid Waste Management Engineering Principles and Management Issues by
George Tchobanoglous, Hilary Theisen and Samual A Vigil, McGraw Hill Publishers
2. Hazardous Waste Management by Michael D. LaGrega, Phillip. L. Buckingham and
Jeffery C Evans, Waveland Press Inc.
.
ELECTIVE – II
BIO NANO TECHNOLOGY
Credits : 3
COURSE OBJECTIVES:
To understand biological nano structureswith characterization and their applications.
To learn about Drug delivery.
To have keen knowledge about diagnostics, Imaging anddevelopment of sensors.
To learn the concept of atomic force microscopy,spectroscopy.
UNIT-I INTRODUCTION TO NANO-BIOTECHNOLOGY:Nanotechnology definition and

concepts; Cellular Nanostructures; Nanopores; Biomolecular motors; Criteria for suitability of
nanostructures for biological applications.
UNIT-II: BASIC CHARACTERIZATION TECHNIQUES; Electron microscopy; Atomic force:

microscopy; Photon correlation Spectroscopy.
UNIT-III NANO STRUCTURES:Thin films; Colloidal nanostructures; Nanovesicles;

Nanospheres; Nanocapsules.
UNIT-IV NANOSTRUCTURES FOR DRUG DELIVERY:Concepts, targeting, routes of delivery

and advantages.
UNIT-V APPLICATIONS OF NANO STRUCTURES:Nanostructures for diagnostics and

biosensors; Nanoparticles for diagnostics and imaging; Nanodevices for sensor development.

• To gain awareness about changes in properties at nano level along with applications.
• An ability to distinguish between different types of nanostructures inbiology.
• An ability to perform spectroscopic techiques.
TEXT BOOKS:
1. Multilayer Thin Films, Editor(s): GeroDecher, Joseph B. Schlenoff Publisher: Wiley-VCH

Verlag GmbH & Co. KGaA ISBN: 3527304401
2. Bionanotechnology: Lessons from Nature Author: David S. Goodsell Publisher: Wiley-Liss
ISBN: 047141719X
REFERENCE BOOKS:
1. Biomedical Nanotechnology Editor: Neelina H. Malsch Publisher: CRC Press

ELECTIVE-III
.
OPEN ELECTIVES
1 Statistical Analysis and Design of

Experiments
2 Operations Research for Engineers
3 Food Bio Technology
ELECTIVE – III
STATISTICAL ANALYSIS AND DESIGN OF EXPERIMENTS
Credits : 3
MGENT : 101(1) Periods/ Week : 3
Course objective
• To learn construction of frequency distributions,measures of central tendency.
• To learn probability distributions
• To learn control charts, correlation and regression.
• To learn time series,parametric tests.
UNIT-I
Introduction– Applications of Statistical Techniques in Chemical Process – Collection of
Primary and Secondary data, Tabulation of data.Construction of Frequency Distributions:
Graphical representation of frequency distributions. (Histogram, Frequency polygon, Frequency
Curve, Ogives)Measures of central tendency : Mean by direct, Short cut methods ; Median and
Mode Calculation in discrete, group data, graphical methods. Measures of dispersion : Range,
With in and Between variations, PMR, Quartile Deviation, Box & Whisker Plots, Outliers,
Mean deviation, standard deviation, variance and coefficient of variation. -– Sampling methods
and sampling errors.
UNIT-II
Probability Distributions :Applications, Problems on Normal distribution , Binomial
distribution and Poisson distribution .Tests of significance: introduction, need, scope, One
tailed and two tailed tests, Interpretation and limitation - ‘t’ test , ‘F’ test & Chi-Square test
Decision Theory : Elements , decision under risk and uncertainty .
UNIT - III.
Control charts: Need , Principle, types, limits, interpretations , selection of control charts –
shifting of mean ,rework, scrap. Correlation: Methods of studying Correlation – Coefficient of
correlation– Rank correlation; Regression : Regression equations , correlation and regression
coefficient , a brief note on Partial and Multiple regression.
UNIT-IV
Time series :components of time series – Measurement of trend – method of least squares,
second degree parabola – measurement by logarithms – shifting the trend origin. Non –
Parametric Tests: ,Kruskal –Wallis test,, a brief note on Duncan’s Multiple Range Test,
Newman-Keuls test, Mann-Whitney U Test . Analysis of variance: elements , Interpretations,
ANOVA of One way , Two way and, observations more than one per cell . Model adequacy
testing .
UNIT-V
Design of Experiments: Introduction, Terminology , Principles , Randomised Block Design
,Completely Randomised Design, Latin Square Design, and Analysis of Co-Variance.
Factorial Experiments: Definitions, Interpretation of main effects and interactions, design with
factors at two levels – Calculation of effects and Analysis of variance – Yate’s algorithm, 2K
factional design in detail ( 22 ,23,24 )- Confounding in Factorial Design a brief note on Response
Surface Methodology, Online and off line quality control Taghuchi methods Experimental
designs for fitting response surfaces Composite Designs. Mixture experiments,

• An ability to construct graphical representations of frequency distributions.
• An ability to interpret test ftest and chi-square test.
• An ability to co relate partial and multiple regressions.
• An ability to measure by logarithms,newman-keuls test.
be answered out of 8 .
Text Book
Design and Analysis of Experiments- Douglas C. Montgomery, 7th edition, 2010 John Wiley
and Sons INC
Reference Book
1.Design and Analysis of Experiments- Douglas C. Montgomery, 7th edition, 2010 John Wiley and Sons INC.
2. Fundamentals of Applied Statistics- S.C.Guptha and V.K. Kapoor , Sultan Chand & Sons , New Delhi, 2012
ELECTIVE-III
OPERATIONS RESEARCH FOR ENGINEERS
Credits :3
MGENT : 101(2) Periods per week :3
Sessionals : 25 Duration of Exam. :3 Hrs.
Examination: 75 Nature of Exam :Theory.
Course Objectives
1.To understand the need and scope of Operations Research for Engineering applications
2.To learn the formulations of different models in Operations Research
3.To understand the concepts of LPP, Assignment and Transportation and their applications for
Engineering process
4.To design a process with all variables and constraints and model it for a specific end use
5. To Plan for the various phases of Project
UNIT -I
Introduction: Definition of OR , Objectives, Scope, Phases and Different models of OR, Role of
Operations Research in Engineering , Essential requirements of a problem
Linear Programming Model : Characteristics, Assumptions, formulations and graphical
solution , Special cases of Graphical solution.
UNIT – II.
Simplex : contents of a simplex problem, types of simplex problem, Maximisation,
Minimization, two-phase method, duality and its objective, writing a dual problem for LPP and
solving by simplex, dual simplex
Assignment Model: objective, types of assignment problems, Hungerian method, profit
maximisation, Airline crew problem, special assignment problem.
UNIT – III.
Transportation model: objectives, types of transportation problems, lowest cost entry method
and north west corner method
Replacement models – waiting line models – optimum replacement of age of items that
deteriorate with time – optimum replacement of items that fail completely
Decision Theory: Need, elements of decision theory, simple problems with Risk and
Uncertainty
UNIT – IV
Queuing systems and their classification – characteristics of queuing systems – problems on
Queuing theory
Inventory Control: Need, Def, Significance types of decisions, types of Inventories,EOQ :
elements, derivation for EOQ, simple problems with and without EOQ, A brief note on
Production Model, Shortages
UNIT – V
Sequencing: Need , Assumptions , Types of problems (n-job on 2 machines, n-job on three
machines and 2 jobs on n-machines
Project scheduling by PERT and CPM – introduction to network analysis – Construction of

network diagrams – calculation of floats- A brief note on Crashing of Networks
Contents beyond the Syllabus

1. Planning of the production mix with different variables and formulation of a model
2. Designing a Allocation model for process with continuous production
3. Planning for the Transportation of Raw materials and Finished product in a production
set up
4. Preparing a Project with feasibility, formulation and Execution plans
5. Planning a productivity schedule using the principles of Queuing and Decision theory
Course Outcomes: ( Graduate to have )

1. An ability to select a model for a specific end use.
2. An ability to design a production /marketing/ personnel case study and modeling it
3. An ability to allocate the minimum resources with maximum returns
4. An ability to design a Transportation model in material handling and transportation
5. An ability to select a project from the alternatives and preparing the PERT
EXAMINATION: Question paper consists 8 Questions selecting atleast one from each unit and
5 to be answered out of 8.
.
REFERENCE BOOKS:
1.Operations Research : Pannerselvam, Tata-Mc GrawHill, 5t Edition ,New Delhi,2013
2.Operations Research – S.K. Kapoor, Chand Publications, New Delhi, 2013
3.Problems and solutions in Operation Research – Man Mohan and P.K.Gupta, Dhanpathi Roy &
sons, New Delhi, 2010
2.PERT and CPM – B.C. Punmia, Dhanpathi Roy & sons, New Delhi, 2012
ELECTIVE-III
FOOD BIO TECHNOLOGY
Credits :3
MGENT : 101(3) Periods per week :3
Sessionals : 25 Duration of Exam. :3 Hrs.
Examination: 75 Nature of Exam :Theory.
Course Objectives
• To understand basic fundamentals of food safety and regulations.
• To learn different types of fermented foods, preservation processing and production of
food.
• To understand enzymatic processes of fruit juices,baking,meat processing.
• To learn regulation of food identity and quality of biotechnology products.
UNIT-1
Fundamentals of Food Safety and regulations,Biotechnology in the evolution of food quality and
standards,Nutritive value of the food,Biotechnology in relation to the Food Industry,Bio
chemicals in Flavor development,Imparting of color,Preservation, Processing and Production of
food,manufacture of HFCP and single cell protein.
UNIT-2
Fermented foods,Dairy products and the role of Biotechnology,meat,Fishery,beverages,Pickles
and Bakery products.Starter Culture development and Process development,Enzymes in the dairy
Industry,Cheese making and Whey processing,Impact of enzyme technology.
UNIT-3
Enzymatic Processing of Fruit juices,Role of enzymes in Baking and Meat Processing,DNA-
based methods for food authentication,Comparative methods for food toxicity testing in Novel
foods.Biological approach to tailor made fats ,Catabolic Processes and oxygen dependent
reactions in food.Applications of genomics technologies in food and nutritional
sciences,Anticancer components in food.
UNIT-4
Regulation of food identity and quality,regulation of biotechnology
products,Foodlabeling,Source,Quantity,Ingredient, and Nutrition Labelling,Nutrient and Quality
Descriptors,Difference between foods and drugs,Health claims for foods,Dietary Supplements.

• An ability to manufacture hfcp and single cell protein
• An ability to handle enzymes in dairy industry cheese making, whey processing.
• An ability to perform comparative methods for food toxicity testing in novel foods.
• An ability to perform food labelling and nutrient labelling, quality descriptors.
Text Books:
1.”Biotechnology Challenges for the Food and Flavor Industry” By LinsayWikkis,Elsiver

Applied Science,1988.
2.FoodBiotechnology,By Roger Angold,Gordon Beech and Taggart,1989.
3.Basic Food Microbiology by GeorgeJBanward,CBS Publishers,1987.
4.Modern Food Microbiology by James M Jay, CBS Publishers,1987.
MOLECULAR BIOLOGY AND GENETIC ENGINEERING LAB

Credits : 2
MBTP : 101 Periods/ Week : 4
Examination : 50 Nature of the Exam : Practicals
LIST OF EXPERIMENTS:
Introduction to Good Laboratory Practices and Regulatory Issues Involved in handling of

GMO’s and Pathogenic MO’s
1. RNA Isolation
• Formaldehyde Gel Electrophoresis
• Quantification of RNA
2. DNA Isolation
• Isolation from Mammalian Cells
• Resolution by Agarose Gel Electrophoresis (AGE)
3. Designing of Gene
• Oligonucleotide primers
• Basic protocols of PCR, RT PCR and AGE
• Restriction Mapping
• Visualization
4. Cloning of Gene using Expression Vector E.Coli
• Isolation of Plasmid DNA
• Restriction Digestion of Vector
• Ligation of Gene in Vector
• Electro-competent Cell preparation
• Transformation of Ligate DNA in E.Coli
5. Characterization of Clone
• Recombinant Plasmid DNA Isolation
• Mobility Shift Assay
• PCR Analysis
• Restriction Digestion Analysis
6. Screening of Clone
• SDS PAGE
• Southern Hybridization
• Northern Hybridization
• Recombinant phage library
• Secondary library screens
7. Sequence analysis
• Preparation of DNA templates
• Functional analysis
• Computational Analysis of DNA and Proteins
• PCR – analysis of products of gels
SEMINAR - I
Credits : 2
MBTP :102 Periods per week : 4
Sessionals : 75 (Report:25; Presentation :50) Nature of Exam. : Report Evaluation
& Oral Presentation
Preparation of a report and presentation of a seminar is intended to investigate the in depth

review of literature to prepare a critical review and to develop confidence for making
presentation
Each student has to prepare and submit a comprehensively typed report in prescribed format on
any topic connected with the field of specialization. The topic shall be chosen in consultation
with the concerned Faculty and Program Coordinator/Head of the Department.
The candidate has to give seminar presentation regarding the work carried out. Evaluation is
done by internal evaluation committee as given below
- Marks for report : 25 max.
- Marks for Seminar talk : 50 max.

BIO TECHNOLOGY)
CBCS SYLLABUS
I YEAR – II SEM
CORE COURSES
Core-4 Computational Techniques in Bio

Technology
Core-5 Protein Engineering and Enzyme
Technology
Core-6 Down Stream Processing in Bio
Technology
COMPUTATIONAL TECHNIQUES IN BIOTECHNOLOGY

MBTT-201 Credits : 3
Sessionals : 25 Periods per Week : 3
Examination : 75 Duration of Exam : 3 Hours
Nature of Exam:Theory
Course Objectives
• To understand graphical representation of biochemical systems-maps.
• To understand stoichiometric network models.
• To learn BLAST,FASTA,MSA,CLUSTAL W.
• To learn genomics and proteomics.
UNIT-I
Introduction To Computational Biology: Need of Computers in Biotechnology Research-
Elementary commands and protocols, Bioinformatics- Introduction, Scope of Bioinformatics,
Applications, Alignment-Local, Global alignment; Gap- Gap penalty; Parametric sequence
alignments-Sub optimal alignments; Comparison of Pair wise and Multiple alignment
UNIT- II
Data bases primary and secondary, Microarrays (DNA,RNA and Protein),Drug discovery, role of
bioinformatics in drug designing, Models of biochemical systems, Specification of S-system
equations simulation and computer aided analysis, Dynamics and its examples,
UNIT-III
Computational Molecular Biology-1
Natural biological principles behind BLAST,FASTA, MSA(Dynamic programming, CLUSTAL
W{Des Higgins}) Karlin-Alshultzl E-values,Matrices PAM,BLOSSUM.
UNIT-IV
Methods In Phylogenic Analysisand Maximum Likelihoods and Models Of Biological Activity
CLADISTICS, PHENETICS,EVOLUTIONARY PHYLOGENETICS, Models of evolution 1)
Jukes and Cantor model 2)Kimura-2-parameter model 3)Logdet method2D and 3D
QSAR/QSPR, and physio-chemical/ADME
UNIT-V
Genomics and Proteomics: Sequence Assembly and gene identification,Homology based gene
prediction,Restriction Mapping analysis,SNPs and application, EST approach.Introduction to
proteins,Proteinidentification,Structure and function determination,Prediction of secondary from
sequence(CATH and SCOP).

An ability to construct a proper map,s-systems.
An ability to perform branched pathway and feed back.
An ability to perform BLAST, FASTA, MSA, PAM, BLOSSUM matrices.
An ability to compare evolutionaryphylogenetics.
Examination: Question paper consists 8 Questions selecting atleast one from each unit and 5 to
Text Books:
1.”Fundamentals of Computers” by V.Rajaraman.
2.”Bioprocess Computations” by T.K.Ghosh.Springler-Verlag Press.
3. Bioinformatics Basics,Applications in biological Science and Medicine-Hooman&co.
4.Bioinformatics: A Machine Learning Approach-P.Baldi,S.Brunak,MIT press,1988.
5.Algorithms on Strings Trees and sequences-Dan Gusfield,Cambridge University Press,1997.
6. Bioinformatics: Methods and Applications-SC Rastogi,NMendiratta&P.Rastogi.
7.S.Sahai,genomics and proteomics,functional and computational aspects,Plenum
publications,1999.
8.DavidW.Mount Bioinformatics-Sequence and Genome analysis.CSHL Press.
PROTEIN ENGINEERING AND ENZYME TECHNOLOGY
Nature of Exam:Theory
Course Objectives
To understand protein structure and folding mechanisms,3D structure of a protein.
To learn molecular graphics in protein engineering.
To learnabout enzymes,structure,specificity,enzyme inhibition.
To understand isolation and purification of enzymes from natural sources.
UNIT-1
Protein structure and folding, Mechanism of folding, Chaperonins and other proteins, shape, size
and confirmation, motifs of protein structure, alpha domain, beta domain, alpha/beta domain,
protein tertiary and quaternary structures, Mathematical principles, Bragg’s Law, Method for
determining 3-D structure of a protein, X-Ray Diffraction, Nuclear magnetic resonance
techniques.
UNIT-II
Strategies for protein engineering ,Random, Site-Directed Catalytic effectivity, structure
prediction and modeling of proteins, molecular graphics in protein engineering, Dynamics and
Mechanics, Drug-protein interaction and design, Protein engineering benefits in Industry and
Medicine.
UNIT-III
Enzymes Classification, Nomenclature, Mechanisms and Kinetics of Enzyme action, Kinetics of
Substrate and multi substrate reaction mechanisms, enzyme structure action and specificity,
Types of enzyme inhibition, estimation of Michaelis-mentor parameters, isolation purification
and characterization of enzymes from natural sources.
UNIT-IV
Enzymes used in biotransformations, regulatory enzymes and their functions,industrial enzymes
and their commercial application of enzymes,immobilization of enzymes and their industrial
applications
UNIT-V
mass transfer effects in immobilized enzyme systems,design of enzyme reactors,design of
enzyme electrodes and their applications as biosensors in industry,health and environment.

An ability to perform nuclear magnetic resonance techniques
An ability to isolate,purify and characterize enzymes useful in health and environment.
An abilty to perform mass transfer effects in immobilized enzymes.
An ability to immobilize enzymes for industrial applications.
Text Books:
1.Lehninger “Principles of Biochemistry” by David .L.Nelson and
Michael.M.Cox,Fourthedition,W.H.FPalmer,Freeman and company,2005.
2.”Protein Engineering” by Moody P.C.E and A.J.Wilkinson.
3.”Enzymes by Trevor palmer,East West press.
4.”Biochemical Engineering Fundamentals” by
James.E.Bailey,David.F.Ollis,secondedition,McGraw Hill
5.”Technological Applications of biocatalysts”, by Butterworth ,BIOTOL series,1995.
6.Physical chemistry : Principles and Applications of Biological Sciences by
Trinoco.I.,Jr.Sauer.K,Wang,J.C&Puglisi,J.D., 4 rthedition,Princeton Hall 2001.
7.”Analytical uses of immobilized enzymes” by G.G.Uilbautt Marcel-Dekker,N.Y.
DOWN STREAM PROCESSING IN BIO TECHNOLOGY
Examination ; 75 Duration of Exam : 3 Hours
Nature of Exam : Theory
Course Objectives
To learn about down stream processing in biotechnology.
To learn physical chemical separation processes in solid and liquid systems.
To learn reverse osmosis, super critical fluid extractions, mechanical separations.
To understand chromatography methods, membrane based separations, dialysis.
UNIT-I
Importance of down stream processing in Biotechnology-Problems, requirement of
purification,Basic concepts of separation technology, Overview of a bioprocess including
upstream and downstream processing.
UNIT-II
Characteristics of biological molecules, Classes of Bio products, Physicochemical basis of
separation,
( New Separation process in modern biotechnology; Separation characteristics of
proteins and enzymes – size, stability & other biological properties; Selection of purification
methodologies.)
Physical Separation process-solid and liquid system, flocculation, coagulation, centrifugation,etc.
Equilibrium Stage process New technologies for cell recovery.
UNIT-III
Separation of intra cellular, extracellular, heat and photosensitive materials, emerging separation
techniques-Dynamic Immobilization.
UNIT-IV
Reverse Osmosis, membrane, membrane technology, pervaporation, super critical fluid
extractions, Flocculation and conditioning of broth; Mechanical separation; Cell disruption,
Protein precipitation and its separation; Aqueous two phase extraction; Adsorption-desorption
processes.
UNIT-V
Chromatographic Methods of separation based on size, charge hydrophobic interactions,
biological affinity methods,etc.,Membrane based separation; Electrophoresis and Electrodialysis;
Crystallization; case studies.

An ability to perform different types of chromatographic methods.
An ability to filter by membranes using micro and ultra filtration
An ability to characterize proteins and enzymes.
An ability to perform purification with different techniques.
Examination : Question paper consists 8 Questions selecting atleast one from each unit and 5 to
be answered out of 8.
Text Books:
1.”Biochemical engineering Fundamentals” 2 nd ed. By J.E.Bailey and D F Ollis,McGraw-Hill.
2.”Bioreactors in Biotechnology-A Practical approach” by A H Scragg
3.”Comprehensive Biotechnology” Vol 2 ed. M.Moo-Young(1985)
4.”Principles of Fermentation technology” by P F Stanbury and A Whittaker, Pergmon press
(1984).
ELECTIVE-IV
PROGRAM ELECTIVES
1 Intellectual Property Rights & Regulatory
Affairs
2 Advanced Oxidation Processes &
Technology
3 Electro Chemical Engineering
ELECTIVE – IV
PHARMACEUTICAL BIOTECHNOLOGY
MBTT-204(1) Credits : 3
C OURSE OBJECTIVES:
To understand Pharmaceutical Biotechnology and their applications.
To learn about Drug interactions.
To have keen knowledge about Drug Metabolism.
To learn the concept of immunity and Immunio Diagnostics .
Unit - I
Introduction to Pharmaceutical Biotechnology:
Origin, definition, scope and importance of Pharmaceutical Biotechnology and their applications:
Microbes in Pharmaceutical Industry: Production of Secondary Metabolites:
Unit – II
Drug Interactions: ADME Properties: Mechanism of Drug Absorption, Drug Distribution, Drug
Metabolism and Drug Excretion:
Unit - III
Probiotics – Development, Role, Functions and Health Effects
Antibiotics – Semi Synthetic penicillin’s, streptomycin and erythromycin as per IP
Hormones – Insulin Production
Enzymes – Amylase and Diastase: Immobilization and their applications in drug manufacture.
Biomass – Lactobacillus sporogenes
Unit - IV
Immunization Products:
Manufacture, Standardization, Storage, Labeling and Specific Applications of the following
Vaccines: Bacterial Vaccines, toxoids, Viral Vaccines, Rickettsial vaccines, Rabies, MMR, BCG,
DPT, Cholers, Hepatitis B and Polio.
Unit – V
Standardization and Storage of the following Passive Immunization products—Anti toxins, Anti
venom, Immune sera and other products related to immunity and Immunio Diagnostics.

• To gain awareness about changes in properties at Drug dosage level along with
applications.
• An ability to Manufacture, Standardization, Storage, Labeling and Specific Drugs .
• An ability to Standardization and Storage of Immunization products .
1. A text book of Pharmaceuticals , A.O. Bentley, 8th Edition, 1982 Bailler Tindall & Co.,
2. The Theory and Practice of Industrial Pharmacy –Leon Lachman , Libermann
3. The Science and Practice of Pharmacy (Vol.I-II)-Remington
4. Biopharmaceutics and Pharmacokinetics-A Treatise-DM Brahmankar, Sunil B Jaiswal.
5. Pharmaceutical Biotechnology-SS Purohit, HN Kakrani & AK Saluja , Student Edn.
ELECTIVE – IV
ADVANCED OXIDATION PROCESSES & TECHNOLOGY
Credits : 3
Course Objectives
• To learn about fundamentals of AOP, ozonation,principles of sono chemistry.
• To understand photochemistry,photolysis,photo oxidation reactions.
• To learn about comparative studies of biodegradability and toxicological studies.
• To learn about textile,pharmaceutical and petroleum industries.
UNIT I
Introduction to AOP,fundamentals of AOPs for Wter and wastewater treatment.
UNIT II
Photoinduced AOP, UV Photolysis H2O2, UV/O3 processes, Ozonation, Fenton processes,
Ultrasound processes and principles of sonochemistry.
UNIT III
Photochemistry, photolysis, fundamentals of semiconductor photocatalyis, photochemical
processes for water and wastewater treatment, photooxidation reactions, photocatalytic
reactions, photo-initiated oxidations, heterogeneous and homogeneous photocatalysis and
kinetic studies.
UNIT IV
Fenton processes: homo and heterogeneous process, effect of system composition and process,
identification of degradation products.
Photoelectrocatalysis process: photooxidation and photomineraliztion of organic matter in
water and air: aqueous systems, substrate oxidation and mineralization, comparative studies of
photo-initiated AOPs, biodegradability and toxicological studies.
UNIT V
Application of AOPs for VOC reduction and odour treatment, case studies – textile,
pharmaceutical and petroleum and petrochemical industries.
• An ability to apply AOP and VOC reduction for odour treatment.

• An ability to do comparative studies of photo initiated AOPs.
• An ability to do biodegradability and toxicological studies.
• An ability to perform fentonprocesse
REFERENCES
• Simon Parsons,Advanced oxidation processes for water and wastewater treatment, IWA
Publishing, 2004.
• Thomas Oppenländer , Photochemical Purification of Water and Air: Advanced Oxidation
Processes (AOPs): Principles, Reaction Mechanisms, Reactor Concepts, Wiley-VCH
Publishing,Published by, 2003.
• Vincenzo Belgiorno, Vincenzo Naddeo and Luigi Rizzo, Water, wastewater and soil
treatment by Advanced Oxidation Processes (AOP), Lulu Enterprises, 2011.
• Harold J.Ratson, Odor and VOC control handbook,Newyork, Mcgraw-hill, 1998.
ELECTIVE – IV
ELECTROCHEMICAL ENGINEERING
Credits : 3
Course Objectives
• To understand mass transfer in electro chemical systems.
• To learn about corrosion,corrosion theories and diffusion controlled corrosion process.
• To learn about electro deposition, electro refining, eloectro polishing.
• To learn about metal finishing cell design.
UNIT I
Review basics of electrochemistry: Faraday’s law -Nernst potential –Galvanic cells –
Polarography, The electrical double layer: It’s role in electrochemical processes –
Electrocapillary curve –Helmoltz layer –Guoy –Steven’s layer –fields at the interface.
UNIT II
Mass transfer in electrochemical systems: diffusion controlled electrochemical reaction- the
importance of convention and the concept of limiting current. over potential, primary secondary
current distribution –rotating disc electrode.
UNIT III
Introduction to corrosion, series, corrosion theories derivation of potential-currentrelations of
activities controlled and diffusion controlled corrosion process. Potential-pH diagram, Forms of
corrosion- definition, factors and control methods of various forms of corrosion-corrosioncontrol
measures- industrial boiler water corrosion control – protective coatings – Vapor phase inhibitors
–cathodic protection, sacrificial anodes –Paint removers.
UNIT IV
Electro deposition –electro refining –electroforming –electro polishing –anodizing – Selective
solar coatings, Primary and secondary batteries –types of batteries, Fuel cells.
UNIT V
Electrodes used in different electrochemical industries: Metals-Graphite –Lead dioxide –
Titanium substrate insoluble electrodes –Iron oxide –semi conducting type etc. Metal finishing-
cell design.types of electrochemical reactors, batch cell, fluidized bed electrochemical reactor,
filter press cell, Swiss roll cell, plug flow cell, design equation, figures of merits of different type
of electrochemical reactors.
• An ability to have basic knowledge in electrochemistry.

• An ability to produce potential-ph diagrams.
• An ability to use electrodes in different electrochemical industries.
• An ability to metal finishing-cell design.
TEXT BOOKS
1. Picket, “ Electrochemical Engineering “, Prentice Hall. 1977.
2. Newman, J. S., “ Electrochemical systems “, Prentice Hall, 1973.
REFERENCES
1. Barak, M. and Stevenge, U. K., “ Electrochemical Power Sources - Primary and Secondary
Batteries” 1980
2. Mantell, C., ” Electrochemical Engineering “, McGraw Hill, 1972.
ELECTIVE-V
DEPARTMENTAL ELECTIVES
1 Environmental Bio Technology
2 Process Flowsheeting
3 Environmental Impact &Assesment
4 Energy Bio Technology
ELECTIVE – V
ENVIRONMENTAL BIOTECHNOLOGY
MBTT – 205(1) Credits : 3
Nature of the Exam : Theory
Course Objectives
To learn different analysis of various microbial processes for biomass production.
To learn physical, chemical, biological characteristics of water.
To study minimum biological and solid retention time.
To understand sludge characteristics and design considerations.
Unit -I
Analysis Of various microbial processes used in production of biomass, primary and secondary
metabolites; Microbial leaching of minerals; Microorganisms in degradation of xenobiotics and
removal of heavy metals;
Unit – II :
Definition of waste. Physical. Chemical and Biological characteristics of waste water, BOD.
COD and TOD their estimation and correlation; BOD progression curve and kinetics; Effect of
Reaction rate of constant on short term BOD, Determination of BOD rate constants; Effect of
Temperature on BOD; Nitrification and Denitrification and their Activated sludge process
(ASP), Biological solid retention time.
Unit –III :
Mixing regime in ASP, Kinetic model of ASP, Sludge volume index (SVI); relation between
recycle ratio and biological solid retention time in ASP, minimum biological and solid retention
time, Aeration system in ASP, Step aeration, extended aeration. Contact stabilization, loading
criteria excess sludge production, sludge viability, O2 requirement in ASP, nutrient requirement
in ASP; separation in secondary clarifier;
Unit IV :
Mass balance in secondary clarifier,-evaluation of kinetic parameters in ASP nitrification and
biological denitrification in ASP:. Anaerobic treatment of wastes, fundamental microbiology,
Process kinetics; Rate limiting step approach, Gas production process design consideration. Case
study; Attached growth of biological treatment process, Trickling filter, anaerobic digestion.
Kinetic relationships, sludge characteristics and design considerations, process meddling and
control, case study: Aerobic digestion - kinetic relationships and design consideration case study.
• An ability to evaluate kinetic parameters inasp nitrification and denitrification.

• An ability to produce primary and secondary metabolites.
• An ability to maintain aeration system in ASP, step aeration, extended aeration.
• An ability to performaerobic digestion .
Text Books and References:`
l. Environmental Biotechnology by Foster C.Fand John Ware DA Ellis Horwood Ltd., 1987
2. Biotechnology and Biodegradation; Advances in Applied B Series Vol.4 By Karnely D,
Chakrabarthy, and-Omen G.S., Gulf Publications Co., London, 1989
3. Bioremediation Engineering: Design and Application, by John T and Cookson Jr., Mc-Graw
Hill Inc., 1985
4. Environmental Biotechnology by Evans and Furlong
ELECTIVE – V
PROCESS FLOW SHEETING

MBTT – 205(2) Credits : 3
Course Objectives
To design flow sheeting, block diagrams,flow design variables.
To understand computer aided flow sheeting,steady state stimulating programs.
To learn heat exchanger networks,area estimates, algorithm stream splitting.
To learn about quick screening of process alternatives, cost diagrams.
Unit – I
Information flow design variables, structure of design problems, flowsheeting –
Definition, block diagrams, stream flow rates and information to be included.
Unit – II
Computer Aided Flow Sheeting, Steady State Simulation Programmes – An overview,
Information Flow Diagrams, Calulations with recycle streams, Split Fraction Concept,
closed recycle systems,Estimation of the Spirit Fraction Coefficients.
Unit – III
Heat Exchanger Networks – Introduction, Minimum Heating and cooling requirements,
MinimumNumberof Exchangers, Area Estimates, design of Minimum Energy Heat Exchanger
Networks Loops and Paths. Reducing the number of Heat Exchangers, a more complete Design
Alogarithm Stream Splitting,
Unit – IV
Heat and power integration, Heat engines, Heat Pumps, Design procedures, Heat and
Distillation HDA Process, Cost diagrams, Conventional approach, Process Alternatives, Cost
diagrams, for complex processes, Quick Screening of process alternatives.
Unit – V
Computer Aided process flow sheet, General structure, As per plus applications. Flow sheet
creation.Input parameters, selected modules such as Heat Exchnagers, Ditillation column
and Reactors.Review of the Hierarchical decision procedure for petrochemical, solid and batch
processes.
• An ability to calculate with recycle streams,split fraction concept

• An ability to design heat and distillation HDA process.
• An ability to general structure flow sheet creation.
• An ability to review of hierarchical decision procedure for petrochemical,solid,batch processes.
Reference Books
1. Chemical Engineering Design – Volume-6, Richarson
2. Conceptual Design of Chemical Process, J.M. Douglas, Mc Graw Hill Book Company,
International Edition. 1988.
3. Basic Principles of Calulations in Chemical Engineering by David M. Himmelblau,
Prentice Hall of India.
4. Process Flow Sheeting by A.W. Westerberg, H.P. Hutchson, R.L.Motard and P.Winter,
5. Cambridge University Press. 1979.
ELECTIVE – V
ENVIRONMENTAL IMPACT ASSESSMENT
MBTT : 205(3) Credits: 3

Sessionals : 25 Periods per Week :3
Course Objective:
• For exposing the students in Impact analysis of pollution in process industries and its assessment
methods and Socio economic Impact assessment.
• To learn Environmental Management and Impact assessment in certain case studies
• To understand environmental management plan,implementation and review.
• To learn sectoral EIA
UNIT-I
Introduction
Historical development of Environmental Impact Assessment (EIA), EIA in Project Cycle, Legal
and Regulatory aspects in India – Types and limitations of EIA – Cross sectoral issues and terms
of reference in EIA – Public Participation in EIA. EIA process- screening – scoping - setting –
analysis – mitigation
UNIT-II
Components And Methods For EIA
Matrices – Networks – Checklists – Connections and combinations of processes – Cost benefit
analysis – Analysis of alternatives – Software packages for EIA – Expert systems in EIA.
Prediction tools for EIA – Mathematical modeling for impact prediction – Assessment of
impacts – air – water – soil – noise – biological –– Cumulative Impact Assessment –
Documentation of EIA findings – planning – organization of information and visual display
materials – Report preparation. EIA methods in other countries
UNIT-III
Socio-Economic Impact Assessment
Definition of social impact assessment, Social impact assessment model and the planning
process, Rationale and measurement for SIA variables, Relationship between social impacts and
change in community and institutional arrangements, Individual and family level impacts,
Communities in transition - neighborhood and community impacts, Selecting, testing and
understanding significant social impacts, Mitigation and enhancement in social assessment.
Environmental costing of projects
UNIT-IV
Environmental Management Plan
Environmental Management Plan - preparation, implementation and review – Mitigation and
Rehabilitation Plans – Policy and guidelines for planning and monitoring programmes – Post
project audit – Ethical and Quality aspects of Environmental Impact Assessment. Post Project
Monitoring.
UNIT-V
Sectoral EIA
EIA related to the following sectors - Infrastructure –construction and housing Mining –
Industrial - Thermal Power - River valley and Hydroelectric – coastal projects-Nuclear Power,
EIA for coastal projects.SEZ, Buildings.
• An ability to design legal and regulatory aspects in india.

• An ability to learn public participation in environmental impact assessment.
• An ability to manage environmental management plan.
• An ability to implement ethical and quality aspects of EIA.
Examination : Question paper consists 8 Questions selecting at least one from each unit and 5
to be answered out of 8.
TEXT BOOK:
1. Lawrence, D.P., Environmental Impact Assessment – Practical solutions to recurrent
problems, Wiley-Interscience, New Jersey, 2003.
REFERENCE BOOKS:
1. Petts, J., Handbook of Environmental Impact Assessment, Vol., I and II, Blackwell
Science, London, 1999.
2. Canter, L.W., Environmental Impact Assessment, McGraw Hill, New York. 1996
3. World Bank –Source book on EIA
ENERGY BIOTECHNOLOGY
MBTT : 205(4) Credits :3

Examination : 75 Duration of Exam : 3 hrs
Course Objective:
• To learn general principles of intermediary metabolism.
• To understand biological energy utilization.
• To learn about biological fuel generation.
• To learn about biodiesel, energy auditing.
Unit -I
Catabolism and the Generation of Biochemical Energy:
General Principles of Intermediary metabolism, regulation of pathways and strategies for
pathway analysis
Bioenergetics - oxidation-reduction concepts, free energy and high energy molecules,
thermodynamic considerations, Electron transport system and ATP, chemo-osmotic drug
coupling.
Biological Energy Utilization: (Respiration): Structure of mitochondria, mitochondrial

metabolism, cellular respiration, relationship of carbohydrate metabolism to other
compounds, Glycolysis, fermentation, formation of acetyl co-A, Krebs cycle, factors
affecting respiration.
Photosynthesis: The ultimate energy resource, oxidative and photophosphorylation.
Unit - III :
Biological Fuel Generation: Microorganisms and energy requirements of Mankind,
Production of non-conventional fuels through Fermentation, Methane (Biogas), hydrogen,
Alcohols and Algal Hydrocarbons.
Sources of different biomass, different species, feed stock, pyrolysis, gasification and
combustion, aerobic and anaerobic bioconversion process, composition and properties of
biogas, biogas plant design and operation, cost analysis and economics of systems
utilization.
Unit - IV :
Biodiesel and Environment: Production, Refinement (Hydrogenation and inter-
Esterification), Strategies for pollution control and Environmental aspects of Energy
utilization, Use of microorganisms in augmentation and petroleum recovery.
Unit –V :
Energy Auditing: Identification for areas of conservation and auditing of energy in a
manufacturing and process industry.
An ability to regulate pathways of oxidation, reduction concepts.

An ability to consider electron transport systems and ATP,chemo-osmotic drug coupling.
An ability to use micro organisms for energy requirements of mankind.
An ability to produce , refinement of environmental aspects.
Examination : Question paper consists 8 Questions selecting at least one from each unit
and 5 to be answered out of 8.

1.”Renewable Energy”, Sorenson.B., Academic Press, 1989.
2.” Source book for farm energy alternatives”, Ritche.J.D. McGraw Hill, 1983.
3.”Technological Applications of Biocatalysts” by Butterworth, BIOTOL Series, 1995.
ELECTIVE-VI
OPEN ELECTIVES
1 Patents & Intellectual Property Rights
2 Industrial Psychology
3 Fuel Cell Technology
ELECTIVE – VI
PATENTS & INTELLECTUAL PROPERTY RIGHTS
MGENT : 201(3) Credits :3

Course Objectives
1.To provide students the concepts of IPR Patents and GMP.
2.To make the student learn about GATT,WTO Indian Regulatory Affairs, WHO, USFDA.
3. To learn how the applications of this is done in process Industries .
UNIT I
Patents • Concept of Patent • Product / Process Patents & Terminology • Duration of Patents-
Law and Policy Consideration Elements of Patentability,- Novelty and Non Obviousness
(Inventive Steps and Industrial Application, Non- Patentable Subject Matter • Procedure for
Filing of Patent Application and types of Applications • Procedure for Opposition, • Revocation
of Patents • Ownership and Maintenance of Patents • Assignment and licensing of Patents •
Working of Patents- Compulsory Licensing • Patent Agent- Qualification and Registration
Procedure. Patent Databases & Patent Information System • Patent Offices in India • Importance
of Patent Information in Business Development • Patent search through Internet, Patent
Databases.
UNIT – II
Preparation of Patent Documents • Lab Notebooks/Log Books/Record Books • Methods of
Invention Disclosures • Patent Application and its Contents • Writing of the Patent Document.
Process for Examination of Patent Application • Publication of Patent Applications • Request for
Examination • Process for Examination & Prosecution • Reissue & Reexamination. Patent
Infringement • Literal Infringement • Doctrine of Equivalence and Doctrine of Colorable
Variation • Contributory Infringement • Defenses to Infringement including Experimental Use,
Inequitable Conduct, Patent Misuse • Legal Aspects (Act, Rules, Procedures). Recent
Developments in Patent System • Software and Business Method Patenting in India & other
Jurisdiction • Patentable Inventions with Special Reference to Biotechnology Products entailing
Creation of New Forms of Life
UNIT – III
IPR(Intellectual Property Rights); Introduction • Meaning, Relevance, Business Impact,
Protection of Intellectual Property • Copyrights, Trademarks, Patents, Designs, Utility Models,
Trade Secrets and Geographical Indications • Bio-diversity and IPR • Competing Rationales for
Protection of Intellectual Property Rights • Introduction to the leading International Instruments
concerning Intellectual Property Rights: the Berne Convention, Universal Copyright Convention,
The Paris Convention, Patent Co-operation Treaty, TRIPS, The World Intellectual Property
Organization (WIPO) and the UNESCO
UNIT – IV
Trademarks • The rationale of protection of trademark as (a) an aspect of commercial and (b) of
consumer rights • Definition and concept of Trademarks • Different kinds of marks (brand
names, logos, signatures, symbols, well known marks, certification marks and service marks) •
Non Registrable Trademarks • Procedure for Registration of Trademarks • Opposition Procedure
• Assignment/Transmission / Licensing of Trademarks • Infringement of Trademarks • Passing
off Action • Offences and Penalties • International Conventions- Madrid Protocol • Dtomain
Names • WIPO Internet Domain Name Process. Copyrights • Nature of Copyright • Works in
which Copyrights subsist (vi) • Author & Ownership of Copyright • Rights Conferred by
Copyright • Assignment, Transmission, Licensing of Copyrights • Infringement of Copyrights •
Remedies & Actions for Infringement of Copyrights • Copyright Societies, Office, Board,
Registration of Copyrights & Appeals • International Conventions • Copyright pertaining to
Software/Internet and other Digital media • Remedies, especially, possibility of Anton Pillar
Injunctive Relief in India
UNIT – V
Industrial Designs : What is a Registrable Design • What is not a Design • Novelty &
Originality • Procedure for Registration of Designs • Copyright under Design • Assignment,
Transmission, Licenses • Procedure for Cancellation of Design • Infringement • Remedies 11.
Geographical Indications • Meaning and Nature • Who are entitled for registration • Conditions
& Procedure for Registration • Offences and Penalties. Protection of Trade Secrets. Key
Business Concerns in Commercializing Intellectual Property Rights • Competition and
Confidentiality Issues, Antitrust Laws • Assignment of Intellectual Property Rights • Technology
Transfer Agreements • Intellectual Property Issues in the Sale of Business • Care & Maintenance
of Confidential Information • Legal Auditing of Intellectual Property • Due Diligence of
Intellectual Property Rights in a Corporate Transaction, Management and Valuation of
Intellectual Property.
Course Outcomes:
1 An ability to evaluate the IPR and Patents.
2 To apply and implement USFDA, WHO, WTO standards.
3 To train and practice the students for IPR and filing of patents.
4
Examination: Five Questions to be answered out of Eight Questions set covering all the Units.
SUGGESTED READINGS:
1. IPR, Neha Publishers & Distributors, ISBN10: 8183871648, ISBN13: 9788183871648

2. Patent Searching: Tools & Techniques,20 Feb 2007 ,David Hunt ), Long
Nguyen , Matthew Rodgers .
3. Das P, Das G. Protection of industrial property rights.
4. Katju SN. Laws and drugs. Law Publishers.
5. Original Laws published by Government of India.
6. Hussain. Law of drugs in India.Patent Law, Fourth Edition (Aspen Treatise) 4th
Edition, Janice M. Mueller.
7. Indian Patents Law – Legal & Business Implications -AjithParulekar, Saritha D’Souza.
Macmillan Publishers India,2006.
The Law of Intellectual Property Rights – Shiv Sahai Singh, Deep & Deep publishers, 2004.
ELECTIVE – VI
.
INDUSTRIAL PSYCHOLOGY
MGENT : 201(2) Credits :3

Course Objectives
1. To understand the concept of Industry, industry atmosphere and its nature
2. To learn about the role of Group Dynamics and its impact on productivity
3. To learn about concept of Industrial Relations and Industrial peace
4. To learn about the types of Supervision and its effects
5. To understand the various levels of Worker participation in Industry
UNIT-I
Introduction to Industrial Psychology-Need and objectives, Scope of Industrial Psychology-
Indian Industrial scenario-Principles of Industrial Psychology-Modes Operandi of Industrial
Psychology- Organisation of Industrial Psychology-Causation of Behaviour
Human Factor at work: Nature of Human factor, Theory of X , Theory of Y ,Rational Vs
Complex Man, Knowledge Vs Wisdom of Worker, Human behavior models (A brief Note)
UNIT-II
Group Dynamics: Concept, Nature and Types of Work- Groups, Significance of Group, Group
norms and Determinants-Group Cohesiveness and factor affecting – Cohesiveness &
Productivity.
Concept of Industrial Relations: Concept , Parties to Industrial relations, Significance of
Industrial peace –Manifestation of Industrial Unrest – Industrial Disputes and Machinery for
prevention and settlement - Integration of Industrial relations with HRD functions
UNIT-III
Supervision & Leadership : Need for Supervision, Types of Supervision, Functions of
Supervisor, Tasks and Processes of Supervision- Span of Supervision-Factors influencing Span
of Supervision and its impact on Organsiation structure – Relationship Strategy for Supervision
Leadership & Leadership Styles: Need for Leader and Leadership, Manager Vs Leader, Styles
of Leadership, Managerial Grid and its impact-Leadership and Group performance.
UNIT-IV
Employees Maintenance: Types of Working environment and Employee Health- Role of
Ergonomics Employee care, Employee Safety, Employee security and legal provisions
Labour Welfare: Concept , scope, significance, Principles of ofLabour Welfare-Agencies of
Labour Welfare, Types of Welfare services, various provisions under Factories Act, Labour
Welfare Machinery
UNIT-V
Employee Counseling: Need, Concept ,Functions and procedure, extent, forms of Counseling,
Causes of Counseling Need, vocational guidance and its principles, Techniques of Counseling.
Workers Participation in Management : Meaning, Objectives, need and Significance ,factors
influencing Participation, Role of Work Committee, JMC, Worker Director, Worker
Participation schemes, Worker Shareholders,
Course Outcome:( Graduate to have )

1.An ability to understand the industrial set up
2. An ability to ensure Industrial peace by understanding the psychology of a worker
3.An ability to excel as good leader and understand the Group dynamics
4An ability to design a suitable labour welfare
5.An ability to Counsel employee and improve his morale .
EXAMINATION: Question paper consists 8 Questions selecting atleast one from each unit and
5 to be answered out of 8.
TEXT BOOK
1.Industrial Psychology – P.K.Ghosh and M.B. Ghorpade, Himalaya Publishing House, Mumbai,
10 th Edition, 2013
REFERENCE BOOKS
1..Human Resource Management- T.N. Chabra,, Dhanpat Rai & Co, New Delhi, 2012
2.Personnel Management & industrial Relations- Tripathi, Sultan Chand & Sons, New Delhi,
2011
ELECTIVE – VI
FUEL CELL TECHNOLOGY
MGENT : 201(3) Credits: 3

Sessionals : 25 Periods per Week :3
Course Objectives
• To understand about fuel cells, their working principle, Types, Design and performance analysis.
• To learn about the fuel cell reaction kinetics, overvoltage, tefel equation.
• To learn about fuel cell characterization, frequency response analysis.
• To learn about fuel cell power plants, balance of plant.
UNIT I
Overview of fuel cells: Low and high temperature fuel cells; Fuel cell thermodynamics - heat,
work potentials, prediction of reversible voltage, fuel cell efficiency.
UNIT II
Fuel cell reaction kinetics - electrode kinetics, overvoltage, Tafel equation, charge transfer
reaction, exchange currents, electro catalysis - design, activation kinetics, Fuel cell charge and
mass transport - flow field, transport in electrode and electrolyte.
UNIT III
Fuel cell characterization - in-situ and ex-situ characterization techniques, i-V curve, frequency
response analysis; Fuel cell modelling and system integration: - 1D model – analytical solution
and CFD models.
UNIT IV
Balance of plant; Hydrogen production from renewable sources and storage; safety issues, cost
expectation and life cycle analysis of fuel cells.
UNIT V
Fuel cell power plants: fuel processor, fuel cell power section (fuel cell stack), power
conditioner; automotive applications, portable applications
Course outcome
After completing the course, student should have learnt:

An ability to haveBasics and workingprinciples of the Fuel cell technology.
An ability to Select the suitable materials for electrode, catalyst, membrane for the fuel cells.
An ability for mass transfer process such as pressure drop and velocity distribution in single cell
as well as stack.
An ability toDesign and stack making process for real field applications
TEXT BOOKS &REFERENCES

1. O'Hayre, R.P.,S. Cha,W. Colella, F.B.Prinz, Fuel Cell Fundamentals,Wiley, NY (2006).
2. Bard,A. J. , L. R., Faulkner,Electrochemical Methods, Wiley, N.Y.(2004) Ref Book.
3. Basu,S.(Ed) Fuel Cell Science and Technology,Springer, N.Y.(2007).
4. Liu, H.,Principles of fuel cells, Taylor & Francis, N.Y. (2006).
5. Fuel cell technology handbook, edited by GregorHoogers, CRC Press 2003.
DOWN STREAM PROCESSING IN BIOTECHNOLOGY LAB

Credits : 2
MBTP : 101 Periods/ Week : 4
Examination : 50 Nature of the Exam : Practicals
LIST OF EXPERIMENTS:
Introduction to Good Laboratory Practices and Manufacturing Practices, Method

Validation and Laboratory Safety Techniques
Upstream Process
1.Media Preparation
-Media Analysis(Purity Check,and Biomass Estimation)

-Media Sterilization
- Validation of Sterilization
2.Optimization of Bioprocess parameters for Growth & Production
3.Stoichiometry of Microbial Growth and Product formation
4.Downstream Processing
1.Cell Separation
- Centrifugation
-Filtration(Cross flow filtration ,Gel filtration,Ultra filtration)
2.Cell Disintegration
- Sonication
-Lyophilization
3.Compound Isolation
-Solvent Extraction
- Salt Precipitation
4.Purification of Protein
- Chromatography (Ion-exchange,Gel Filtration,Affinity etc..)
-Electrophoresis
-Crystallization
5.Drying (Vaccum,Spray and Freeze)
6. Physico-Chemical Characterization
-SDS PAGE
-Coomassie Blue Staining
-Silver Staining
-TLC,GC,HPLC
- Spectroscopy (UV-Visible,IR,Mass,NMR,X-Ray diffraction etc..)
-IEF
-Western Blot
SEMINAR - II
Credits : 2
MBTP :202 Periods per week : 4
Sessionals : 75 (Report:25; Presentation:50) Nature of Exam.: Report
Evaluation & Oral Presentation
Preparation of a report and presentation of a seminar is intended to investigate the in depth

review of literature to prepare a critical review and to develop confidence for making
presentation
Each student has to prepare and submit a comprehensively typed report in prescribed format on
any very recent topic connected with the field of specialization. The topic shall be chosen in
consultation with the concerned Faculty and Program Coordinator/Head of the Department.
The candidate has to give seminar presentation regarding the work carried out. Evaluation is
done by internal evaluation committee as given below
- Marks for report : 25 max.
- Marks for Seminar talk : 50 max.

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M Tech (BCE&BT) CBCS Scheme and Syllabus Approved in

Academic Council meeting held on 17/04/2017

M.TECH (BIOCHEMICAL ENGINEERING &

SCHEME OF INSTRUCTION AND EXAMINATION – (CBCS)

FIRST YEAR FIRST SEMESTER

SYLLABUS Catego PERIODS / WEEK MARKS Duration of Credits

Elective –I Elective-II Elective-III

FIRST YEAR SECOND SEMESTER

Elective –IV Elective-V Elective-VI

SCHEME OF INSTRUCTION AND EXAMINATION – (CBCS)

SECOND YEAR FIRST SEMESTER

Code Course Category Periods/day Assessment Marks Credits

Library Practica Internal Ext Interna Ext.

SECOND YEAR SECOND SEMESTER

Code Course Category Periods/day Assessment Marks Credits

Library Practical Internal Ext Interna Ext.

M.TECH (BIOCHEMICAL ENGINEERING &

Core-1 Molecular Biology and Genetic

MOLECULAR BIOLOGY AND GENETIC ENGINEERING

• To understand the concept of molecular biology, Dna replication, transcription and

Introduction To Molecular Biology

Molecular biology, Basic concepts in Molecular Biology, Classification, Isolation,

General properties and classification of plasmids, Significance of Plasmids, Host controlled

Introduction To Genetic Engineering

Basic concepts of genetic engineering, Overview of recombinant DNA technology-concept,

• COURSE OUTCOMES(Graduate to have)

1.MolecularCloning,Smabrook et al,Cold Spring Harbour.

2.Essentials Of Molecular Biology,Malacinski and Freifelder Jones and BartleLt publishers.

CELL CULTURE TECHNIQUES IN BIO TECHNOLOGY

• To understand structure of prokaryotic and eukaryotic organisms.

history, Laboratory requirements and General techniques, Introduction, Requirements,

• COURSE OUTCOMES(Graduate to have)

1. Molecular Biotechnology: Primrose.

BIO REACTOR DESIGN & PROCESS ENGINEERING

• To learn about different types of bioreactors.

Product Formation in Microbial Cultures: Growth associated and non-growth associated

Definition of Bioreactor, types of reactors-batch, plug flow reactor(PFR),continuous stirred tank

Stirred Tank batch bioreactor: For all enzymes, cell cultures.

Continuous Stirred Tank Bioreactor-Operation of mixed reactor-enzyme reactions in mixed

Multiphase Bioreactors-packed bed with immobilized enzymes or microbial cells.

• COURSE OUTCOMES(Graduate to have)

1.Blanch H.W. and DoughlasS.Clark,BiochemicalEngineering,CRC press,1997.

2.Micheal L Shuler and Fikretkargi,BioprocessEngineering,Prentice-Hall of India Pvt Pld,2008.

3.StanburyP.F.Whitaker, Principles of fermentation technology,Elseiver India Pvt,Ltd,2007.

4.ArthurT.Johnson,Biological Process Engineering,AnAnological Approach to fluid

5.PaulineM.Doran, Bioprocess Engineering Principles.Academic Press,1995.

6.D.G.Rao Introduction to Biochemical engineering Tata Mc Graw-Hill,2005.

• COURSE OUTCOMES(Graduate to have)

SCIENCE & TECHNOLOGY OF BIOMATERIALS

COURSE OUTCOMES(Graduate to have)

• An ability to have knowledge on atomic force microscope

Text Books and References:

Configuration/Operation of Rotating Packed Bed (RPB) Reactor

COURSE OUTCOMES(Graduate to have)

• An ability to use different types of reactors

Text Books and References

3 KameliaBoodhooo, Adam Harvey (Editors) Process Intensification Technologies for Green

MODERN ANALYTICAL TOOLS & TECHNIQUES

Principles and applications of analytical Tools and techniques in Biotechnology.

Microscopy-Phase contrast, Confocal, SEM&REM microscopic principles in biology.

Fermentation process- Gas analysis for O2 and CO2.

Flow Injection Analysis,Computational data acquisition of bioprocesses.Online Bioprocess

Chromatographic Techniques & Tools- Column (Affinity & Ion-Exchange