“Laboratory Safety”

Harold John A. Borlaos, RMT

I. Chain of infections
1. Infectious agents
2. Reservoirs
3. Exit or Portal of Exit
4. Mode of transmission
5. Entry or Portal of Entry
6. Susceptible host

II. Hand-Hygiene
Hand Contact/ Direct Contact
 It is the primary method of infection transmission.

Alcohol-based cleaners
 It can be used when hands are not visibly contaminated.
 They are not recommended after contact with spore-forming bacteria.

Proper Handwashing:
1. Wet hands with warm water. Do not allow parts of body to touch the sink.
2. Apply soap, preferably antimicrobial.
3. Rub to form a lather, create friction, and loosen debris. Thoroughly clean between the
fingers and under the fingernails for at least 20 seconds; include thumbs and wrists in the
cleaning. (2 Happy Birthday Songs)
4. Rinse hands in a downward position to prevent recontamination of hands and wrists.
5. Obtain paper towel from the dispenser.
6. Dry hands with paper towel.
7. Turn off faucets with a clean paper towel to prevent contamination.

REMEMBER:
 ACID should always be added to WATER.
 DISINFECTANT: Sodium hypochlorite
_________
 All electrical equipment must be grounded
with three pronged plugs.
III. Material Safety Data Sheets/ MSDS
 Document containing information of the chemicals used in the laboratory.
 All employees have a right to know about all chemical hazards present in their
workplace.

The NFPA diamond

 NFPA stands for: National Fire Protection Association

You Were Born Right

IV. Waste segregation

 BLACK
GREEN
YELLOW
RED
ORANGE

 Hazard associated with breakage in

centrifuge:________________________
 HEPA (High Efficiency Particulate Air) filter can remove
particulates of at least ________ in size.

V. Magnitude of hazard:
0 Normal
1 Slightly hazardous
2 Hazardous
3 Extreme danger
4 Deadly

VI. Type of fires

 CLASS A

CLASS B

CLASS C

CLASS D

CLASS K

What is the most common type of fire extinguishers? ___________________

END
"Through hard work, perseverance and a faith in God, you can live your
dreams." - Ben Carson

WELCOME FUTURE RMT, LABAN LANG