Operating Manual Washer 430

Instructions for Use for
Washer 430
Document Part No.: 45610087

2004-12
Document Revision: 2.2
Firmware Level: 4.x
WARNING
CAREFULLY READ AND FOLLOW THE INSTRUCTIONS FOR USE BEFORE
OPERATING THE INSTRUMENT.
bioMérieux bv reserves the right to make changes to this document without

further notification. This document is thought to be fully consistent with the
product it describes. bioMérieux bv does not assume liability for the contents of
this document.
If this appliance is not used according to the description in this document ,
bioMérieux bv does not assume liability for any consequent effects.
We would appreciate any comments on this publication.
Manufacturer
bioMérieux bv
Boseind 15
5281 RM Boxtel
The Netherlands
Telephone: 0031 411 654911
FAX: 0031 411 654201
E-mail: gcsimmunoboxtel@eu.biomerieux.com
Copyright Information
The information in this publication is of a proprietary nature. No part of this
publication can be reproduced, stored in a retrieval system, translated,
transcribed, or transmitted in any form or by any means without prior written
permission of bioMérieux bv.
Copyright © 2004 bioMérieux bv
All rights reserved.
Declaration for EU Certificate
A Declaration of Conformity Certificate is available upon request.
Intended use of Instrument
See chapter 2.1 Introduction.
About the Instructions for Use
This document describes the basic Washer 430, which is designed to wash
microplates. It is intended as a reference and instruction for the user.
This document instructs how to:
• Install the instrument
• Operate the instrument
• Program the wash procedure
• Define the wash parameters
• Clean and maintain the instrument
2 Instructions for Use for Washer 430 No. 45610087 Rev. No. 2.2 2004-12
Warnings, Cautions and Notes
There are four types of informational notices used in the Instructions for Use
(IFU). These notices highlight important information or warn the user of a
potentially dangerous situation. The notices are as follows:
Note
Gives helpful information.
Caution
Indicates a possibility of instrument damage or data loss if instructions are
not followed.
WARNING
INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY, LOSS OF
LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS ARE NOT
FOLLOWED.
WARNING
INDICATES THE POSSIBLE PRESENCE OF BIOLOGICALLY
HAZARDOUS MATERIAL. PROPER LABORATORY SAFETY
PRECAUTIONS MUST BE OBSERVED.
2004-12 Instructions for Use for Washer 430 No. 45610087 Rev. No. 2.2 3
Symbols
Manufactured by
Date of manufacturing
Conformité Européenne
Biohazardous
Abbreviations
Abbreviation
A Ampere
ANSI American National Standards Institute
C Celsius
CE Conformité Européenne
cm Centimeter
CV Coefficient of Variation
F Fahrenheit
Hz Hertz
kg Kilogram
l; L Liter
µl Microliter
ml Milliliter
mm Millimeter
REF Reference Number/ Order Number
s Second
SBS Society for Biomolecular Screening
SN Serial Number
T Träge (Slow Blow Fuse)
TYPE Name and type of instrument
V Volts
VA Volt ampere
Table of Contents
1. Safety
1.1 Instrument Safety .......................................................................... 1-1
2. General
2.1 Introduction.................................................................................... 2-1
2.2 Instrument Specifications............................................................. 2-2
2.2.1 Microplates ...................................................................................... 2-3
2.3 Instrument Description ................................................................. 2-4
2.3.1 Rear Panel Connections.................................................................. 2-5
2.3.2 Connection Diagrams ...................................................................... 2-6
2.3.3 Rear Panel Connections.................................................................. 2-9
2.4 Instrument Options ..................................................................... 2-11
2.4.1 Liquid System Diagrams................................................................ 2-13
2.5 Instrument Software Features .................................................... 2-14
2.5.1 Software Menus............................................................................. 2-14
2.6 Washing Sequence Modes ......................................................... 2-15
2.6.1 Washing Modes............................................................................. 2-15
2.6.2 Aspirating Positions ....................................................................... 2-16
2.7 Rinse Modes ................................................................................ 2-17
3. Installation Procedure
3.1 Unpacking Procedure ................................................................... 3-2
3.2 Power Requirements ..................................................................... 3-3
3.3 Installation Procedure ................................................................... 3-4
3.3.1 Installing the Instrument .................................................................. 3-4
3.3.2 Connecting the Solution Tubes ....................................................... 3-4
3.3.3 Fitting and Connecting the Liquid Sensors (Optional) ..................... 3-5
3.3.4 Power Connections ......................................................................... 3-6
4. Operating Instructions
4.1 Switching the Instrument On........................................................ 4-1
4.2 Mode Selection Menu .................................................................... 4-2
4.3 Priming Procedure ........................................................................ 4-3
4.4 Operating Instructions .................................................................. 4-5
4.4.1 Performing a Wash Procedure ........................................................ 4-5
4.4.2 Procedure to Wash Individually Selected Strips .............................. 4-7
4.5 Error Messages............................................................................ 4-10
4.5.1 Standard Instrument ...................................................................... 4-10
4.5.2 Instrument with additional Liquid Sensor (Option A)...................... 4-11
4.5.3 Instrument Fitted with Manifold Detection Sensors (Option B) ...... 4-13
4.5.4 Instrument Fitted with Fill Verification (Option B)........................... 4-13
4.6 End of Operation ......................................................................... 4-15
5. Programming Procedure
5.1 Introduction.................................................................................... 5-1
5.2 Procedure for Defining a New Program....................................... 5-2
5.2.1 Process Step: Aspirate .................................................................... 5-3
5.2.2 Process Step: Wash ........................................................................ 5-3
5.2.3 Process Step: Dispense .................................................................. 5-4
5.2.4 Process Step: Soak ......................................................................... 5-4
5.2.5 Process Step: END.......................................................................... 5-5
5.3 Editing a Defined Program............................................................ 5-6
5.3.1 Changing the Type of Defined Process Step................................... 5-6
5.3.2 End Programming Procedure .......................................................... 5-6
5.4 Show Program ............................................................................... 5-7
5.5 Clear Program................................................................................ 5-8
6. Adjust Menu
6.1 Introduction.................................................................................... 6-1
6.2 Plate Type....................................................................................... 6-3
6.2.1 Round Bottom Plates....................................................................... 6-4
6.2.2 Flat Bottom Plates ........................................................................... 6-5
6.2.3 Movement Diagrams ....................................................................... 6-6
6.3 Adjust Liquid Sensors .................................................................. 6-7
7. Maintenance and Cleaning

7.1 Cleaning Procedures..................................................................... 7-1
7.1.1 Outer Surface of the Instrument Housing ........................................ 7-1
7.1.2 Display Window Cover .................................................................... 7-1
7.1.3 Cleaning Pumps and Tubes ............................................................ 7-1
7.1.4 Manifold Cleaning............................................................................ 7-2
7.1.5 Cleaning Waste Bottles ................................................................... 7-3
7.1.6 Liquid or Foam Spills ....................................................................... 7-4
7.2 Manifold Removal.......................................................................... 7-5
7.2.1 Replacing the Manifolds .................................................................. 7-6
7.2.2 Replacing the Plate carrier .............................................................. 7-8
7.3 Replacing the Main Fuses ............................................................ 7-9
7.4 Preventive Maintenance Plan ..................................................... 7-10
7.4.1 Daily............................................................................................... 7-10
7.4.2 Weekly........................................................................................... 7-10
7.4.3 Every Six Months........................................................................... 7-10
7.4.4 Yearly (Service Engineer Required) .............................................. 7-10
7.5 Rinse Modes ................................................................................ 7-11
7.5.1 Rinse: Day ..................................................................................... 7-11
7.5.2 Rinse: Night ................................................................................... 7-12
7.5.3 Rinse: Auto .................................................................................... 7-12
7.6 Instrument Disinfection .............................................................. 7-13
7.6.1 Disinfection Procedure .................................................................. 7-13
7.7 Disposal of Instrument................................................................ 7-15
7.7.1 Introduction.................................................................................... 7-15
7.7.2 Disposal of Packing Material ......................................................... 7-15
7.7.3 Disposal of Operating Material ...................................................... 7-15
7.7.4 Disposal of the Washer 430 .......................................................... 7-16
8. Trouble Shooting
8.1 Introduction.................................................................................... 8-1
Index
1. Safety
1. Safety
1.1 Instrument Safety
1. Always follow basic safety precautions when using this product to reduce the
risk of injury, fire or electrical shock.
2. Read and understand all information in the IFU. Failure to read, understand
and follow the instructions may result in damage to the product, injury to
operating personnel or poor instrument performance.
3. Observe all STOP and CAUTION statements in the IFU.
4. Never open Washer 430 while the instrument is plugged into a power
source.
5. Observe proper laboratory safety precautions, such as wearing protective
clothing and using approved laboratory safety procedures.
Note
Suitable Compact Microplates
The Washer 430 allows to process flat-bottom or round-bottom
microplates in 96-well format typically used for EIA, conforming to the
standard defined by the Society of Biomolecular Screening (see
chapter 2.2.1 Microplates). Other plate types in 96-well format such as
deep-well plates, PCR-plates and filtration plates cannot be used with
the Washer 430
Note
Suitable Strip-plates
The Washer 430 allows to process flat-bottom or round-bottom strip-
well plates in 96-well format, typically used for EIAs, showing a similar
height and footprint as the 96-well microplates listed above.
To achieve proper wash-performance with strip-well plates ensure
that:
• all strips have been properly inserted into the frame of the strip-
plate to give a level surface
• the actual position of the strips within a strip-plate matches the
position of the strips as defined in program used for
processing. See chapter 4.4.2 Procedure to Wash Individually
Selected Strips.
2004-12 Instructions for Use for Washer 430 No. 45610087 Rev. No. 2.2 1-1
1. Safety
Caution
Be careful when using strip-plates that the strips are positioned
in the microplate as defined in the program used for processing,
otherwise spilling can occur and the instrument may become
contaminated. (see chapter 7.1 Cleaning Procedures).
WARNING
ADJUSTMENT OF WASHER 430 TO A CORRESPONDING MICROPLATE
TO ENSURE PROPER WASH PERFORMANCE IT IS MANDATORY TO
ADJUST THE WASHER 430 TO THE TYPE / MANUFACTURER OF
MICROPLATE OR STRIP-PLATE USED. THIS ALSO APPLIES FOR ANY
PRE-DEFINED PLATE FILE, THAT WILL ALWAYS CONTAIN AVERAGE
PLATE PARAMETERS ONLY, THAT HAVE TO BE VERIFIED WITH THE
CORRESPONDING PLATE TYPE AND IF NECESSARY CORRECTED
BEFORE PUTTING THE WASHER 430 INTO USE.
IF THIS ADJUSTMENT PROCEDURE IS NOT PERFORMED PROPERLY,
THIS MIGHT RESULT IN HIGH LEVELS OF RESIDUAL VOLUME LEFT PER
WELL, AS WELL AS IN INSUFFICIENT WASHING OF THE WELLS AND
MAY SERIOUSLY AFFECT ASSAY PERFORMANCE.
FOR DETAILS ON HOW TO ADJUST THE WASHER 430 TO THE TYPE OF
MICROPLATE OR STRIP-PLATE USED, SEE CHAPTER 6. ADJUST MENU
IN THIS DOCUMENT.
WARNING
WASTE BOTTLE - FILLING LEVEL
MAKE SURE THAT THE FILLING LEVEL OF THE WASTE BOTTLE IS
ALWAYS KEPT BELOW THE MAXIMUM LEVEL INDICATED ON THE
BOTTLE TO AVOID POTENTIAL OVERFLOW. AS THE CONTENTS OF
WASTE BOTTLE ARE POTENTIALLY INFECTIOUS, WEAR PROTECTIVE
CLOTHING (GLOVES, LAB COAT AND SAFETY GLASSES) WHEN
EMPTYING / HANDLING A WASTE BOTTLE. FOLLOW LOCAL STANDARD
OPERATING PROCEDURES ON HOW TO TREAT POTENTIALLY
INFECTIOUS MATERIAL FOR DISPOSAL.
1. Safety
Note
For on-line monitoring of liquid levels in wash buffer-bottles, rinse-bottle
and waste-bottle an optional Liquid Level Sensor System
is available for the Washer 430.
See Chapter 2.4 Instrument Options for further information.
WARNING
WITH SPECIFIC WASH BUFFERS THAT SHOW A STRONG TENDENCY
OF FOAMING, EMPTY THE WASTE BOTTLE AS SOON AS THE FOAM-
LEVEL HAS REACHED THE MAXIMUM FILLING LEVEL INDICATED ON
THE WASTE BOTTLE. ADDITIONALLY ADD A COMMERCIALLY
AVAILABLE ANTI-FOAMING AGENT (SUCH AS SILICONE OIL) TO THE
EMPTY WASTE BOTTLE TO HELP REDUCE FOAMING.
IF FOAMING CONTINUES TO BE A PROBLEM WE RECOMMEND TO
SWITCH TO A LARGER WASTE BOTTLE (NOT PROVIDED WITH THE
WASHER 430) AND ADDITIONALLY INCREASE THE CONCENTRATION
OF ANTI-FOAMING AGENT IN THE WASTE BOTTLE. TO HELP
FACILITATE BREAK-DOWN OF THE FOAM IN THE WASTE BOTTLE,
CAREFULLY SWIRL THE WASTE BOTTLE FROM TIME-TO-TIME TO
IMPROVE MIXING BETWEEN FOAM LAYER AND ANTI-FOAMING
AGENT.
REFILL ANTI-FOAMING AGENT AFTER EMPTYING WASTE BOTTLE.
FOR EXAMPLE WHEN USING THE WACKER ANTI-FOAM EMULSION
SE47 (WACKER ARTICLE CODE 21640582) THE RECOMMENDED
CONCENTRATION IS 1ML OF ANTIFOAMING AGENT FOR 1 LITER OF
WASTE SOLUTION.
FOR ANTIFOAMING AGENTS FROM OTHER MANUFACTURERS USE
CONCENTRATIONS AS RECOMMENDED BY CORRESPONDING
MANUFACTURERS.
2. General
2. General
2.1 Introduction
The Washer 430 is a microprocessor-controlled washer for strip-wise processing
of microplates in the 96-well format. The Washer 430 is a general purpose
laboratory instrument that is designed for professional use only and should only
be operated by trained personnel.
The instrument is fitted with an 8 way manifold, which is fitted with one aspirating
and one dispensing needle per well.
Note
12 and 16 way manifolds are only available on special request for the
Washer 430.
The instrument can use up to nine software defined plate types that control the
dispensing and aspirating positions for round or flat bottomed plates.
The dispensing and aspirating speeds can also be selected from five set speeds.
Standard Washer 430 Instrument
• 8-way manifold, 1 inlet channel, 1 waste channel, Liquid Sensor System 1
Factory-Installed Options
• Option A: 8-way manifold, 4 channels (3 inlet and 1 waste),
Liquid Sensor System 1 and 2
• Option B: 8-way Fill verification manifold,1 inlet channel, 1 waste
channel, Liquid Sensor System 1
• Option A & B: 8-way Fill verification manifold, 4 channels (3 inlet and
1 waste), Liquid Sensor System 1 and 2
Caution
If the instructions given in this document are not correctly performed, the
instrument may be damaged or the procedure may not be correctly
performed and the safety of the instrument cannot be guaranteed.
Caution
The wash results obtained with the Washer 430 are influenced by the correct
use of the instrument, according to the instructions given in this IFU, as well
as the liquid compounds used (reagents, wash buffer, chemistry). The
instructions for use, storage and other manipulations in connection with
samples or reagents have to be strictly followed. Taking this fact into
consideration, results must be interpreted carefully.
Caution
Before the instrument is installed and switched on,
it should be left to stand for at least three hours, so there is
no possibility of condensation causing a short circuit.
Caution
Before washing procedures are started, make sure that the microplate
position A1 is inserted correctly.
2. General
2.2 Instrument Specifications

Note
Instrument specifications have been defined using Greiner F flat-bottom
compact 96-well microplates and may vary using other 96-well flat
bottom plates or 96-well strip-plates.
The table below lists the physical specifications for the instrument. All
specifications listed below are based on the following instrument settings:
Dispensing speed: from 1 - 5 (standard setting is 3)
Aspirating rate: from 1 - 5 (standard setting is 3)
PARAMETERS CHARACTERISTICS
General
Display unit Liquid Crystal Display with two rows of sixteen digits
Keyboard Four key membrane keyboard
Number of dispensing Up to four channels
channels
Manifold types 8 way manifold
Variable
Volume of solution dispensed 50 - 3000 µl in 50 µl steps for washing
50 - 400 µl in 50 µl steps for dispensing
Dispensing accuracy CV: <= 4% over the plate
(300µl dispensing volume, dispensing rate: 3).
Residual volume Crosswise aspiration: <=2 µl per well
Single aspiration: <= 5 µl per well
(Asp. rate 5, Asp. time 4 s., Asp. speed 10mm/s)
Interface All connected devices must be approved and listed as per
EN 60950, UL 1950 or CSA C22.2 No. 950 for Data
Processing Devices.
Serial RS 232 C 9600 Baud (fixed)
Power
Supply Auto sensing for the settings
100 - 120 V or 220 - 240 V, 50 / 60 Hz
Consumption < 65 VA
Main fuse 2 x 1.6 T A (slow blow)
2. General
Physical
Outside dimensions Width : 28,5 cm
Depth: 39,5 cm
Height: 22,0 cm
Weight 8.2 kg
Environmental
Ambient temperature
Operation 15°C to 35°C (59°F to 95°F)
Non-operation -10°C (14°F) to 43°C (109°F)
Storage -40°C to 70°C (-40°F to 158°F)
Relative Humidity
Operation 20 % to 90 % non condensing
Non-operation 5 % to 95 %
Storage 5 % to 95 %
Others
Overvoltage category II
Pollution degree 2 (IEC / EN 61010-1)
Method of disposal Contaminated waste
Linear Shaking
Mode Shaking Speed
1 2 3
Low 1.2 mm 4 Hz 7.5 Hz 11.0 Hz
Normal 1.7 mm 2 Hz 5.0 Hz 8.0 Hz
High 2.6 mm 2 Hz 3.5 Hz 5.0 Hz
The instrument conforms to the current legal norms of the European Community.
A Declaration of Conformity Certificate is available upon request.
2.2.1 Microplates
Only 96-well format microplates (v-shaped, flat and round bottom including
strip-wells) that are in accordance with the ANSI/SBS standards can be used with
the Washer 430. Microplates supplied by bioMérieux are in accordance with the
ANSI/SBS standards.
PARAMETERS CHARACTERISTICS
Max. overall plate height 14.35 mm ± 0.76 mm
(0.5650 inches ± 0.0299 inches)
Footprint Length 127.76 mm ± 0.5 mm
(5.0299 inches ± 0.0197 inches)
Width 85.48 mm ± 0.5 mm
(3.3654 inches ± 0.0197 inches)
Pitch size (center to center) 9.0 mm (0.3543 inches)
Bottom shape V-shaped, Flat and Round bottom
2. General
2.3 Instrument Description

The diagram below shows the main components of the instrument:
Fig. 2-1 Washer 430
Manifold Arm
Manifold
POWER
Power ONLED
Keyboard and
Display Unit
Waste Sink
Plate Support
Guide Bar
Keyboard and Display Unit

The Washer 430 is operated via a keyboard and a control panel consisting of a
two row, sixteen digit Liquid Crystal Display (LCD) unit.
The top row of the display unit is used for messages and the bottom row for the
key functions or options.
A Light Emitting Diode (LED) in the center of the letter O of the word POWER
indicates when the washer is switched ON.
Fig. 2 The Keyboard and Display Unit
Key 1 Key 2 Key 3 Key 4

POWER
Po w er
ON LED
2. General
2.3.1 Rear Panel Connections

The instrument has the following connections on the back panel.
figure 2-2 Standard Washer 430 / Option B:
Mains Power
Socket
Inlet 1
Fuse
Compartment
ON/OFF
Switch
Waste
Tubing
Bottle Holder with Serial RS 232 for
(red)
Liquid Sensor for Remote Control
each bottle
figure 2-3 Option A / Option A & B:
Mains Power
Socket
Fuse
Compartment
ON/OFF Inlets for channels 1 - 4

Switch
Bottle Holder with Serial RS 232 for Waste

Liquid Sensor for Tubing
Remote Control (red)
each bottle
All connected devices must be approved and listed with regulations

EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices
2. General
2.3.2 Connection Diagrams

Washer 430 / Option B
Channels Color Liquid
1 Green Wash liquid
2 Gray Rinse solution
W Red Waste solution
Washer 430 / Option A / Option A & B

Channels Color Liquid
Bottle holder 1 1 Green Wash liquid 1

2 Gray Wash liquid 2
W Red Waste solution
Bottle holder 2 3 Blue Wash liquid 3

4 Violet Wash liquid 4
Yellow Rinse solution
2. General
Fig. 5 Washer 430 / Option B
Marked
red
Maximum
Inlet filling level of 2
Computer for Marked Rinse filter
green solution liters as
Remote Control
not included
indicated on
bottle
grey red
Waste
Wash green Bottle
Liquid
Bottle Holder with liquid

sensor for each bottle
2. General
Fig. 6 Washer 430 and Option A / Option A & B
Input Input Input Input

1 2 3 4
Marked
Marked red purple
Marked
blue
Computer for
Remote Control
not included
Marked green Maximum

filling level of
Marked grey 2 liters as
indicated on
bottle
Inlet
Inlet filter Inlet
filter
filter
Wash Wash Waste Wash Wash
Rinse
Liquid 1 Liquid 2 Bottle Liquid 3 Liquid 4
solution
grey red yellow purple
Bottle holders with

liquid sensor for each bottle blue
green
2. General
2.3.3 Rear Panel Connections

The instrument has the following connections on the back panel:
Fig. 2-4 Washer 430 - Rear Panel
On/Off
Switch
Fuse
Compartment
Main Power
Socket RS 232 Inlets for
Channels 1-4 Waste Tube
Liquid Sensor
System
All connected devices must be proved and listed with regulations

EN 60950, UL 1950 or CSA C22.2 No. 950 for Data Processing Devices
WARNING
MAKE SURE THAT THE FILLING LEVEL OF THE WASTE BOTTLE IS
EMPTYING / HANDLING A WASTE BOTTLE. FOLLOW LOCAL
STANDARD OPERATING PROCEDURES ON HOW TO TREAT
POTENTIALLY INFECTIOUS MATERIAL FOR DISPOSAL.
2. General
WARNING
WASTE BOTTLE - FOAMING
WITH SPECIFIC WASH BUFFERS THAT SHOW A STRONG TENDENCY
OF FOAMING, EMPTY THE WASTE BOTTLE AS SOON AS THE FOAM-
LEVEL HAS REACHED THE MAXIMUM FILLING LEVEL INDICATED ON
THE WASTE BOTTLE. ADDITIONALLY ADD A COMMERCIALLY
AVAILABLE ANTI-FOAMING AGENT (SUCH AS SILICONE OIL) TO THE
EMPTY WASTE BOTTLE TO HELP REDUCE FOAMING.
WARNING
IF FOAMING CONTINUES TO BE A PROBLEM WE RECOMMEND TO
SWITCH TO A LARGER WASTE BOTTLE (NOT PROVIDED WITH THE
WASHER 430) AND ADDITIONALLY INCREASE THE CONCENTRATION
OF ANTI-FOAMING AGENT IN THE WASTE BOTTLE. TO HELP
FACILITATE BREAK-DOWN OF THE FOAM IN THE WASTE BOTTLE,
CAREFULLY SWIRL THE WASTE BOTTLE FROM TIME-TO-TIME TO
IMPROVE MIXING BETWEEN FOAM LAYER AND ANTI-FOAMING
AGENT.
REFILL ANTI-FOAMING AGENT AFTER EMPTYING WASTE BOTTLE.
FOR EXAMPLE WHEN USING THE WACKER ANTI-FOAM EMULSION
SE47 (WACKER ARTICLE CODE 21640582) THE RECOMMENDED
CONCENTRATION IS 1ML OF ANTIFOAMING AGENT FOR 1 LITER OF
WASTE SOLUTION.
FOR ANTIFOAMING AGENTS FROM OTHER MANUFACTURERS USE
CONCENTRATIONS AS RECOMMENDED BY CORRESPONDING
MANUFACTURERS.
2. General
2.4 Instrument Options

A trained service engineer can install the instrument with the following options:
Note
That it may not be possible to upgrade to some options on certain
instruments. Contact your local service representative for details.
Option A : Upgrade from 1 inlet channel to 4 inlet channels

combined with additional Liquid Sensor System.
The Liquid Sensor System is able to detect when
the solution bottles are empty and when the waste
bottle is full. The weight indicator is inside the bottle
holder. The standard Washer 430 instrument is
equipped with one unit and the additional unit can
be ordered as part of Option A.
Field upgrade for option A is available from serial

number 6520001 onwards.
Option B : Fill Verification option (8 channel manifold only) is

available from serial number 6520070 onwards.
This function allows to actively check during an
overflow wash step, if wash buffer is dispensed into
every single well of a 96 well plate.
It is based on conductivity measurement between
the dispensing needles and aspirating needles
(special manifold required). The conductivity of the
wash buffer has to be within a range of 5 mS / cm
to 30 mS / cm.
Please note that it is possible to combine option A

and B in the instrument.
Note
The Fill Verification Option is possible from firmware level 3.23 onwards.
Factory installation required!
Firmware Update Consisting of the Download software, the latest version of

the firmware, as well as 5 language software files
(English, German, French, Spanish and Italian) that can
change the on-board language of the Washer 430 (from
firmware level 2.23 onwards) in the field.
2. General
List of Parts
Note
Products from this list can be ordered by the user.
A service engineer is not required.
Article Number Description
Spare Parts
45610067 Fuse 1.6A, 5 x 20 MM, SLOWBLOW (Net Entry)
45610073 Tubing, Prime Valve (5 / Package)
45610077 Tubing Set, External
45610078 Plate Carrier Complete
45610090 Cable Interface, Washer to PC
45610105 Plate Cover, Manifold
45610107 Filter, Inlet Tubing (5 / Package)
45610255 Tube Support
45610257 Cable Interface, Washer to Bottle Holder
Tools
45610084 Maintenance Kit
45610085 Aspirate Needle Cleaning
45610086 Dispense Needle Cleaning
45610087 Instructions for Use for Washer 430
45610088 Download-QcCol Disks
45610126 Instructions for Use for WinWash Clinical Washer 430

Software
45610140 Plate, Greiner F (For QC)
45610156 Operating Manual for Download & QcCol
45610158 WinWash Clinical, Washer 430 Software CD-ROM
Others
45610071 Manifold, 8 Channel, Standard
45610081 Bottle, Wash (Green)
45610082 Bottle, Waste (Red)
45610083 Bottle, Rinse (Gray)
2. General
2.4.1 Liquid System Diagrams

Fig. 7 Washer 430 / Option B
Manifold
Dispense
Marked blue Solenoid Valve
Dispensing Pump
Marked red
Marked
red
Aspirating Pump Solenoid Valve
Fig. 8 Washer 430 and Option A / Option A & B

Manifold Dispense Solenoid
Marked blue Valve
Marked red Dispensing Pump
Marked
red
Aspirating Pump
Channel select
Solenoid Valves
2. General
2.5 Instrument Software Features

The instrument has the following software features:
• 30 user definable washing programs, stored under the numbers 1 to 30.
• Up to 60 processing steps per program, each processing step can be
identical or different from the previous step.
• 3 Washing Modes, Dilution Washing, Bottom Washing and Overflow
Washing.
Set using the Plate type parameters.
• Soak Time selectable from 1 to 999 seconds.
• Software selectable Dispensing Speeds
• Software selectable Aspirating Speeds
• Software selectable Bottom position, for aspiration close to the bottom of
the wells.
• Three Rinse Modes, the different rinse modes can be used to select how the
instrument is rinsed before it is left to stand or switched off.
• Automatic Microplate Centering, the microplate is automatically centered
before the start of the washing program.
• Programmable Strip Selection, the program can be defined so that the
strips to be washed can be selected before starting the washing procedure.
2.5.1 Software Menus

The instrument can be used in the following software menus:
Run Selection of the Run procedure to perform the selected program
Prime Priming procedure for each channel
Rinse Rinsing procedure
Program Selection of the Programming procedure, to define the required
program
Clear Procedure to clear a program from the instrument's memory
Show Procedure to show the parameters for a program
Adjust Procedure to adjust the instrument parameters
Service Selection of the Service submenu
For service engineer only
2. General
2.6 Washing Sequence Modes

The instrument can wash a microplate using the following sequence modes:
Plate Mode Each process step is performed on all of the strips sequentially,
before proceeding to the next process step. The entire plate is
processed for the defined soaking time (as opposed to the Strip
Mode).
Strip Mode The entire Wash program is performed on a strip before
proceeding to the next strip. Each individual strip is processed for
the defined soaking time, before proceeding to the next strip.
2.6.1 Washing Modes

The instrument can use the following washing modes to wash a microplate:
Overflow Washing Overflow washing consists of a simultaneous
aspiration and dispense step. It creates a circular
flow of wash buffer in the well.
Bottom Washing Washes only defined segments of the well
according to the selected aspiration position.
Dilution Washing Dilution washing consists of a sequence of
aspiration and dispense steps.
Fig. 2-5 Washing Positions

Overflow Washing
Bottom Washing
Dilution Washing
Aspiration step Dispense step
2. General
2.6.2 Aspirating Positions

To ensure that all the wash solution is correctly aspirated from the wells, the
manifold must be positioned so that the aspirating needles are correctly
positioned in the wells for round-bottom, v-shaped bottom or flat-bottom well
microplates.
All settings are defined in the Plate Type setting option of the Adjust menu.
With round or v-shaped bottom wells, the aspirating needles are placed in the
middle of the wells.
'V' Shaped Wells
Round Bottom Wells
Fig. 2-6 Aspirating Positions

With flat bottom wells, the instrument performs crosswise aspiration.
The aspirating needles are set at two positions on the bottom of the wells (front
edge and back edge).
Aspirate position 1 Aspirate position 2
Fig. 2-7 Crosswise Aspiration for Flat Bottom Wells
WARNING
MICROPLATE OR STRIP-PLATE USED. THIS ALSO APPLIES FOR ANY PRE-
DEFINED PLATE FILE, THAT WILL ALWAYS CONTAIN AVERAGE PLATE
PARAMETERS ONLY, THAT HAVE TO BE VERIFIED WITH THE
CORRESPONDING PLATE TYPE AND IF NECESSARY CORRECTED BEFORE
PUTTING THE WASHER 430 INTO USE.
IF THIS ADJUSTMENT PROCEDURE IS NOT PERFORMED PROPERLY, THIS
MIGHT RESULT IN HIGH LEVELS OF RESIDUAL VOLUME LEFT PER WELL,
AS WELL AS IN INSUFFICIENT WASHING OF THE WELLS AND MAY
SERIOUSLY AFFECT ASSAY PERFORMANCE.
FOR DETAILS ON HOW TO ADJUST THE WASHER 430 TO THE TYPE OF
MICROPLATE OR STRIP-PLATE USED, SEE CHAPTER 6. ADJUST MENU OF
THIS DOCUMENT.
2. General
2.7 Rinse Modes

The instrument has three rinse modes to flush the liquid system and to prevent
needle blockages. This mode is for use before the instrument is left to stand or is
switched off at the end of operation.
The rinse modes are:
Rinse: Day
Rinse: Night
Rinse: Auto
See 7.5 Rinse Modes for description of the rinse modes and how they are used.
The instrument is shipped in one carton, which contains:
• Instrument with a built in 8 way manifold and a plate support
• Accessory package which contains:
Two screws and Hexagon socket wrench 2,5 mm
Instructions for Use for Washer 430
Mains power cable - USA & Europe
Cleaning needles
2 spare fuses
Solution tube
25/9 pin converter for communication cable
Spare manifold front plate
bioMérieux break-four microtiterplate
Greiner-Microplate (flat bottom)
Instructions for Use for Software WinWash, Operating Manual for Download
& Qc Col (including 2 disks)
Patch cable
Tube support
RS 232 cable
O-Seals
Final check record
Packing list
• Option B:
3 x 2.5 l bottles (rinse, waste, wash), 1 Liquid Sensor System unit
• Option A / Option A & B:
5 x 2.5 l bottles (1 x rinse, 1 x waste, 3 x wash), 2 Liquid Sensor System
units
Fig. 1 The packaging for the Washer 430 and the Options.
Standard Washer 430 / Option B Option A / Option A & B
Bottles Bottles (Packed on top of each other)
Liquid Sensor Washer Liquid Sensor Washer

System System
430 430 With
options
Accessory package
Accessory package
3.1 Unpacking Procedure

1. Visually inspect the container for damage before it is opened.
Report any damage immediately.
2. Select a location for the instrument that is flat, level, vibration free, away
from direct sunlight, and free from dust, solvents and acid vapors. Allow 10
cm distance between the back of the instrument and the wall or any other
equipment.
3. Place the carton in an upright position and open it.
4. Lift the instrument out of the carton and place it in the selected location.
5. Visually inspect the instrument for loose, bent or broken parts.
Report any damage immediately.
6. Compare the serial number on the rear panel of the instrument with the
serial number on the packing slip.
Report any discrepancy immediately.
7. Check the instrument accessories against the packing list.
8. Save packing materials for future use.
3.2 Power Requirements

The instrument is designed to operate at either 100 - 120 volt or 220 - 240 volt.
No voltage setting is required as the washer automatically senses the supplied
voltage.
The washer requires 2 x T 1.6 A / 250 V (slow blow) fuses.
WARNING
TO PREVENT THE RISK OF FIRE, THE MAIN FUSES SHOULD ONLY BE
REPLACED WITH THE SAME TYPE AND RATING OF FUSE.
3.3 Installation Procedure
Caution
3.3.1 Installing the Instrument

• Unpack the instrument and check the contents.
See 3.1 Unpacking Procedure.
• Connect the solution tubes to the inlet connectors.
See below 3.3.2 Connecting the Solution Tubes.
• Setup instrument options if included. See 2.4 Instrument Options.
• Connect instrument to the power supply. See 3.3.4 Power Connections.
• Define the plate parameters in the Adjust menu. See 6. Adjust Menu.
• Program the wash procedure. See 5. Programming Procedure
3.3.2 Connecting the Solution Tubes

The solution tubes are connected onto the inlet connectors on the back panel of
the instrument. The instrument is supplied with four meters of tubing that can be
cut to the required length (maximum 1 meter).
Fig. 3-1 Connecting the Solution Tubes
Option A and Option A & B Standard Washer 430 and Option B
Inlets for channels 1 - 4 Inlet for channel 1
Waste Tube Waste Tube

red red
3.3.3 Fitting and Connecting the Liquid Sensors (Optional)

Connect the detector unit cable into the appropriate socket in the back panel of
the instrument. The liquid sensor system can be extended to control up to four
different liquid bottles and one waste bottle.
Fig. 3-2 Connecting the Liquid Sensor
rinse liquid
waste yellow
gray red 4
purple
liquid 1 liquid 3
green blue
Standard Option A /
Washer 430 A&B
and Option B
Liquid Sensor
System Connector
Fix the tube support in the bottle holder as shown below.
Tube support
Insert the tubes and connect the bottle holder to the instrument.
3.3.4 Power Connections
Caution
Ensure that the on / off switch in the back panel of the instrument is in the off
position.
Insert the mains power cable into the mains power socket on the rear of the
instrument.
4.1 Switching the Instrument On
Ensure the instrument has been correctly installed and the power cable is
connected into the power cable socket in the back panel of the instrument.
Switch on the instrument, using the on/off switch in the back panel of the
instrument.
The initialization procedure is performed and, depending on the washer type, one
of the following initialization messages is displayed:
WASHER 430
V4.xx
Version V X.x is the instrument's software version.
If the Rinse: Night rinsing procedure was not performed before the instrument
was switched off, the following message is displayed:
You should Rinse
no yes
Select yes and then select the Rinse: Night procedure.
After a rinsing procedure has been selected, the instrument proceeds to the
standby mode and the following message is displayed:
Run XX yyyyyyyy
- + other yes
Where XX is the displayed program number and yyyyyyyy the program name.
Caution
The instrument must be primed before it can be used. Please ensure that the
dispensing and aspiration pumps are not run for longer than a few minutes
without liquid or they will be damaged.
Caution
At the end of each working day, perform the Rinse: Night procedure with
distilled water to ensure the proper performance of the Washer 430 and to
prevent needles from becoming blocked, for details see 7.1 Cleaning
Procedures.
4.2 Mode Selection Menu

The Mode Selection menu has the following submenu selections:
Run XX:yyyyyyy
- + other yes
Selection of the Run procedure
to perform the selected program
Prime: Ch1
- + other yes
Priming procedure for each channel
Rinse: Soak
- + other yes
Rinsing procedure – see 8.5 Rinse
Modes.
Prog XX:
- + other yes
Selection of the Programming procedure,
to define the required program
ClearXX:yyyyyyy
- + other yes
Procedure to clear a program from
the instrument's memory
Show XX:yyyyyyyy
- + other yes
Procedure to show parameters
for a program
Adjust Menu
other yes
Procedure to adjust the instrument parameters
Service
other yes
Selection of the Service submenu
For service engineers only
See 7.5 Rinse Modes
4.3 Priming Procedure
WARNING
BEFORE THE INSTRUMENT CAN BE USED, ALL DISPENSING
CHANNELS NEEDED FOR THE WASH STEP MUST BE PRIMED TO FILL
THE DISPENSE SYSTEM WITH THE REQUIRED SOLUTION.
IF THE PRIMING PROCEDURE IS NOT PROPERLY PERFORMED, THIS
MAY RESULT IN INSUFFICIENT WASHING OF THE WELLS AND MAY
SERIOUSLY AFFECT ASSAY PERFORMANCE.
ENSURE THAT THE BOTTLE OF PRIMING SOLUTION IS ALWAYS FULL
AT THE BEGINNING OF THE PRIMING PROCEDURE AND CHECK THAT
THE INLET FILTERS IN THE LIQUID TUBES ARE CLEAR.
Caution
Ensure that the dispensing and aspiration pumps are not run for longer
than a few minutes without liquid or they will be damaged.
Caution
Do not use the instrument to aspirate or dispense any acidic solutions
as this could damage the instrument.
The priming procedure is performed using the following procedure:
Run XX yyyyyyyy
- + other yes
Prime Ch1 Select required channel to be primed

- + other yes
Prime Solution ? Required solution in bottle and inlet

exit yes
tube inserted into solution
Waste Bottle OK? Waste bottle empty

exit yes
Prime ChX Y sec Priming procedure being performed

Stop
Priming Procedure
performed
Run XX yyyyyyyy Instrument ready to wash microplate

- + other yes
WARNING
MAKE SURE THAT THE LIQUID LEVEL OF THE WASTE BOTTLE IS
4.4 Operating Instructions

4.4.1 Performing a Wash Procedure
Insert the microplate to be washed into the plate carrier and ensure that the
microplate is correctly oriented for the type of manifold fitted.
Orientation for the 8 way manifold
Fig. 4-1 Fitting a Microplate

The operating procedure is dependent on the instrument options and the program
settings.
Caution
Before washing procedures are started, make sure that
the microplate position A1 is inserted correctly.
Note
Adjustment of Washer 430 to a corresponding microplate
To ensure proper wash performance it is mandatory to adjust the Washer 430 to
the type / manufacturer of microplate or strip-plate used. This also applies for
any pre-defined plate file, that will always contain average plate parameters
only, that have to be verified with the corresponding plate type and if necessary
corrected before putting the Washer 430 into use.
If this adjustment procedure is not performed properly, this might result in high
levels of residual volume left per well, as well as in insufficient washing of the
wells and may seriously affect assay performance.
For details on how to adjust the Washer 430 to the type of microplate or strip-
plate used, see chapter 6. Adjust Menu of this document.
Note
Suitable Compact Microplates
standard defined by the Society of Biomolecular Screening
(see chapter 2.2.1 Microplates). Other plate types in 96-well format such as
deep-well plates, PCR-plates and filtration plates cannot be used with the
Washer 430
Note
The Washer 430 allows to process flat-bottom or round-bottom strip-well
plates in 96-well format, typically used for EIAs, showing a similar height
and footprint as the 96-well microplates listed above.
To achieve proper wash-performance with strip-well plates ensure that:
• all strips have been properly inserted into the frame of the strip-
processing. See chapter 4.4.2 Procedure to Wash Individually
Selected Strips.
Caution
in the microplate as selected in the program, otherwise spilling
can occur and the instrument may become contaminated.
(see chapter 7.1 Cleaning Procedures).
Caution
When the instrument is busy, do not touch the manifold.
After the instrument has been used the manifold may be infectious!
4.4.2 Procedure to Wash Individually Selected Strips
Run XX Select required program

- + other yes
Select Strips NO* Select Strips YES**
The 12 strips of an 8 way

manifold are represented by Y
Nr.of strips: XX 1: NYYNNNNNYNNN
(yes) or N (no).
- + exit yes < > chg yes Select required strips using the
cursor keys (selected strip is
underlined) and press chg key to
change the strip’s setting
E.g. NYYNNNNNYNNN means
th
that the 2nd, 3rd and 9 strips
will be washed.
Channel X primed? Required channel primed
no yes
Priming
procedure
Plate inserted ? Plate inserted

exit yes
Waste Bottle OK? Waste bottle empty

exit yes
Procedure
performed
Run XX yyyyyyyy Remove the microplate from plate

- + other yes instrument ready to wash next
* Select Strips NO: Only the total number of used strips can be given. Used
strips must be positioned next to each other, starting from column 1.
** Select Strips YES: Individual positioned strips can be selected.
Note
• all strips have been properly inserted into the frame of the strip
processing.
Caution
in the microplate as selected in the program,
otherwise spilling can occur and the instrument
may become contaminated with infectious material.
(see chapter 7.1 Cleaning Procedures).
Caution
When the instrument is busy, do not touch the manifold.
After the instrument has been used the manifold may be infectious!
Fill Verification
The Fill Verification option is a method of checking the filling of the wells in a
microplate. Possible errors with the dispensing system as well as the aspiration
system can be recognized.
The Fill Verification only works with the OVERFLOW wash procedure.
The wash buffer forms an electrical connection between the dispensing needles
and the aspirating needles. As soon as the wash liquid touches the aspirating
needles the electric circuit is closed.
Manifold
Dispensing needle
Aspirating needle
Well filled with wash buffer
The following errors can be detected using this method.

• Fill Dispense Error
• Fill Aspiration Error
• Fill Function Error
Fill Dispense Error
The error messages Inadequate wash, Check disp path are displayed.
These error messages appear if some of the needles are dispensing no or too
little solution. Possible errors could be a result of blocked dispensing needles,
bubbles in the dispensing channels, empty liquid bottles or the dispensing pump
no longer functions.
If no connection between the dispensing needles and the relevant aspirating
needles is made at least 30 µl before the end of the dispensing step, the wash
program will be interrupted.
Fill Aspiration Error
The error message Fill Asp. Err. is displayed.
In order to prevent the wells from overflowing, the instrument checks when the
individual aspirating needles come into contact with the wash solution.
If this happens before 200 µl has been dispensed into the wells the program is
stopped immediately. This can occur when the well was not or not completely
aspirated, due to a blocked aspirating needle / tube or the general aspiration
being too weak.
Fill Function Error

The error message Fill Funct. Err is displayed.
Before each wash procedure and each row the program checks whether there is
an electrical connection between the dispensing and aspirating needle(s). If a
connection exists, the dispense and aspiration errors can not be detected and the
program is interrupted immediately or will not even be started
The underside of the manifold must then be cleaned, as the deposits from the
wash buffer can produce an electrical connection between the needles, which
could prevent the Fill Verification option from functioning correctly.
Test Conditions
The following conditions are required for the Fill Verification to function flawlessly.
• The conductance of the wash buffer must be between 5 mS/cm and
30 mS/cm.
• The amount of wash liquid dispensed must produce at least 100 µl overflow.
• At least 450 µl should be dispensed with an Overflow Position slightly above
the upper well limit.
Conductance of some Standard Wash Buffers

Most of the commercial wash buffers lie in the mentioned conductance ranges.
The conductance is proportional to the wash buffer concentration.
The following values were measured using a normal dilution:

Wash buffer SIGMA: 1 tablet in 200ml - 14.7 mS/cm
Phosphate buffered saline Tablets:
Wash buffer bioMérieux: 1:25 - 13.7 mS/cm
Phosphate buffer concentrate:
4.5 Error Messages

4.5.1 Standard Instrument
Program not defined
If the selected program has not been defined, the following message is displayed:
Program not def
exit
Press exit to remove the message and return to the standby mode
Run XX yyyyyyyy
- + other yes
Select a defined program that is to be performed.
Wrong Manifold
If the selected program requires a different type of manifold than the manifold that
is fitted to the instrument, the following message is displayed:
WRONG MANIFOLD
exit
Press exit and the instrument returns to the standby mode.
Either replace the manifold for the required type of manifold or re-program the
procedure for the type of manifold that is fitted.
If the correct type of manifold is fitted, check the setting for the fitted manifold in
the Adjust menu.
If either the Manifold Detection or Fill Verification option is enabled ensure that
the ribbon cable is in the correct connector, see 7.2.1 Replacing the Manifolds.
This message may also be displayed, if the Fill Verification or Manifold Detection
options are enabled but the required parts are not fitted to the instrument. In this
case please call your local representative.
Transport Error
If the instrument cannot move the plate carrier, the following message is
displayed:
Init-Err. CONT?
retry yes
Press yes (exit) to remove the error message and the instrument returns to the
standby mode.
Check that the plate carrier is correctly inserted and that the plate carrier system
is clear.
4.5.2 Instrument with additional Liquid Sensor (Option A)

Waste Bottle Full at Start
When the selected program is confirmed, the instrument checks if the waste
bottle is full. If it is full, the following message is displayed:
Wastebottle Full
yes
Press yes to remove the message and return to the start and the following
message is displayed:
Run XX
- + other yes
Empty the waste bottle and restart the program.
Waste Bottle Full during Procedure
If the waste bottle becomes full during the washing procedure, the following
message is displayed after the instrument has completed the washing procedure:
Wastebottle Full
yes
Note
The instrument does not stop a procedure when the waste bottle becomes
full. Therefore, ensure that the waste full sensor is calibrated, so that there
is enough space in the waste bottle to accept the remaining solution.
(See 6. Adjust Menu)
Press yes to remove the message and the instrument proceeds to the standby
mode and the following message is displayed:
Run XX
- + other yes
Empty the waste bottle and the instrument is ready to start the next procedure.
Caution
The Washer 430 cannot end the washing procedure.
Please end the test manually or dispose of the microplate!
Caution
Waste Bottle - Foaming
The liquid level sensor is unable to detect foam, therefore it is important
that the waste bottle is emptied as soon as the foam-level has reached the
maximum filling level indicated on the waste bottle.
Note
Waste bottle - Foaming
With specific wash buffers that show a strong tendency of foaming, it is
recommended to add a commercially available anti-foaming agent (such
as silicone oil) to the empty waste bottle to help reduce foaming.
If foaming continues to be a problem we recommend to additionally
increase the concentration of anti-foaming agent in the waste bottle.
To help facilitate break-down of the foam in the waste bottle, carefully
swirl the waste bottle from time-to-time to improve mixing between foam
layer and anti-foaming agent.
Refill anti-foaming agent after emptying waste bottle. For example when
using the Wacker Anti-foam emulsion SE47 (Wacker article code
21640582) the recommended concentration is 1ml of antifoaming agent
for 1 liter of waste solution.
For antifoaming agents from other manufacturers use concentrations as
recommended by corresponding manufacturers.
Liquid Bottle Empty

If the liquid bottle is empty before a program is started, the following message is
displayed:
Liq. Empty Ch: X
yes
Fill the liquid bottle and press yes to remove the message and continue with the
program.
4.5.3 Instrument Fitted with Manifold Detection Sensors

(Option B)
Manifold Arm Error
If the manifold arm cannot be lowered correctly due to obstruction of its normal
movement, the following message is displayed:
Manif. arm Error !
Exit
• Press Exit to remove the error message and return to the standby mode.
• Check that the microplate is correctly inserted into the plate carrier.
• Check that the manifold arm is not blocked.
• Check if the needles are catching on the side of the microplate, use the
Adjust menu to adjust the positions for that particular type of microplate.
4.5.4 Instrument Fitted with Fill Verification (Option B)
Liquid Not Dispensed

During the Wash procedure
If the instrument detects that one or more needles are not dispensing solution or
non-conductive liquid is being used, the following error messages are scrolled on
the display:
Inadequate Wash!
Exit
Check Disp. Path

Exit
• Press exit to remove the error message and return to the standby mode.
• Check that the reagent bottle has enough solution and the inlet tube is
fitted into the solution.
• Use the Prime menu to fill the liquid system with solution.
• Check that the dispensing needles are clear.
Caution
Fill Communication Error

If the fill verification board does not respond, the following error message is
displayed:
Fill Comm. Err.!
Exit
Switch off the instrument to clear the display. Ensure that the small ribbon cable
and the interface cable (from the mainboard) are connected correctly to the Fill
Verification board. Switch the instrument on again.
Contact the service engineer to disable the Fill Verification option in the
Service menu and to correct the problem.
Fill Function Error
If the fill verification board needle status can not be deleted, the following error
message is displayed:
Fill Funct.Err.!
Exit
Press exit to remove the error message and return to the standby mode.
The problem is caused by an electrical conducting liquid connection from the
dispensing to the aspirating needles on the manifold. The underside of the
manifold must then be cleaned using, for example, distilled water.
Contact the service engineer to disable the Fill Verification option in the
Service menu and to correct the problem.
Fill Aspiration Error
When the well is not correctly aspirated the following error is displayed:
Fill Asp. Err.!
Exit
The problem is caused by a blocked aspirating needle / tube or the aspirating
pump may not be functioning correctly. Use the cleaning needles to clean the
aspirating needles (see 7. Maintenance and Cleaning) or replace the pump.
Contact the service engineer to correct the problem.
Caution
WARNING
AFTER THE INSTRUMENT HAS BEEN USED THE MANIFOLD MAY BE
INFECTIOUS!
BEFORE WORKING ON THE MANIFOLD IT MUST BE THOROUGHLY
DISINFECTED!
(SEE CHAPTER 7.6 INSTRUMENT DISINFECTION)
4.6 End of Operation

At the end of operation, the instrument can be either left switched on with the
manifold in the rinsing solution or switched off.
Instrument Left Switched On
Use the following procedure to leave the instrument switched on with the manifold
soaking in the distilled water at the end of operation:
Place the wash solution tube into a bottle containing distilled water.
Use the Rinse: Night rinsing procedure to rinse the wash system.
(see 7. Maintenance and Cleaning).
When the rinsing procedure is completed the following message is displayed:
Soaking
Stop
If the manifold is to be soaked overnight, do not switch the instrument off.

Instrument Switched Off
If the instrument is to be switched off at the end of operation the Rinse: Day
rinsing procedure should be performed to prevent the needles becoming blocked.
Place the wash solution tube into a bottle containing distilled water.
Use the Rinse: Day rinsing procedure to rinse the wash system.
When the rinsing procedure is completed, switch the instrument off.
5.1 Introduction
The instrument can store up to thirty programs, which contain the following
parameters:
Name Name for the program, up to eight characters long
Plate The plate type number (1 - 9)
Manifold The type of manifold that is to be used (8 way manifold only)
Disp. Rate The speed at which the solution is dispensed
Asp. Rate The speed at which the solution is aspirated
Crosswise Selection if crosswise aspiration is to be used if the plate
type has flat bottom wells.
Select Strips Select if the number of strips or individual strips can be
selected.
Mode The selection of which operating mode is to be used
(plate or strip)
Process Steps Each of the programs can contain up to a maximum of sixty
different or equal processing steps in a number of cycles.
The following processing steps are available:

• Aspirate: Aspirate solution out of the wells
• Wash: Aspirate and dispense a solution
• Dispense: Dispense a solution
• Soak: Leave the solution in the wells for a time, with or without
shaking.
• End: End the programming procedure for this cycle.
5.2 Procedure for Defining a New Program

Prog XX: Select required program to be defined
- + other yes
Name: Enter the required name for the program

890 ↵ ABCDE (up to eight characters)
Plate: 1 Select required plate type.(1 – 9)

- + exit yes Refer to 6.2 Plate Type.
Manifold Type: 8 Select the type of manifold to be used

- + exit yes (Only the 8-way manifold is available
for the Washer 430)
Disp Rate: 3 Select the dispensing speed (1 - 5 or drip)

- + exit yes
Select the aspirating speed (1 - 5 )

Asp Rate: 2
- + exit yes
Plate type:
Plate type: flat bottom
round bottom
Crosswise: NO Select if crosswise aspiration is to be used

- + exit yes Crosswise aspiration only for flat bottom plates
Sel. Strips: NO Select if strips are to be selected

- + exit yes
Mode: Plate Select required operating mode

- + exit yes (strip or plate)
C:1 P:1 END Select required process step

- + other yes
Aspirate: Aspirate solution out of the wells

Wash: Aspirate and dispense a solution
Dispense: Dispense a solution
Soak: Leave the solution in the wells for a time with or without shaking.
End: End the programming procedure for this cycle.
5.2.1 Process Step: Aspirate
C:X P:Y Asp. Select and confirm Aspirating step

- + other yes
Asp.Time: 4 sec Select required aspirating time

- + exit yes (1 - 20 seconds)
Asp.Speed: Select required aspirating speed

- /+ exit yes (1 - 20 mm/sec)
The speed of the needles when lowered into the wells.
Select next type of step

C:X P:Y END C:X = cycle number
- + other yes P:Y = process step number for this cycle
5.2.2 Process Step: Wash

This process step is used to aspirate solution out of the wells and simultaneously
dispense and aspirate the solution.
C:X P:Y Wash Select and confirm Wash step

- + other yes
AspSpeed: 10mm/s Select required aspirating speed

- + exit yes (1 - 20 mm/sec)
The speed of the needles when lowered into the wells.
Dispense Overfl Select required Dispensing position

- + exit yes (overflow or bottom)
Channel: 1 Select the channel to be used

- + exit yes (1 - 2 or 1 - 4 if additional channels are
installed)
Volume: 300µl Select the volume of solution to be used

- + exit yes (50 - 3000 µl in increments of 50 µl)
(50 - 400 µl in increments of 50 µl drip
mode)
C:X P:Y END
Select next type of step
- + other yes
5.2.3 Process Step: Dispense
C:X P:Y Disp Select and confirm Dispensing step

- + other yes
Select required dispensing position

Dispense Overfl
- + exit yes (overflow or bottom)
Select the channel to be used

Channel: 1
- + exit yes (1 - 2 or 1 - 4 if additional channels are
installed)
Select the volume of solution to be used

Volume: 400µl
- + exit yes (50 - 400 µl in increments of 50 µl)
C:X P:Y END Select next type of step

- + other yes
5.2.4 Process Step: Soak
C:X P:Y Soak Select and confirm Soak step

- + other yes
Soaktime: 10sec Select required soaking time

- + exit yes (1 - 999 seconds)
Shake-Speed: NO Select required shaking speed

- + exit yes (No, Linear: Linear shaking
Select the shake speed: 1, 2 or 3.
Confirm with yes. Select the shake mode: low,
medium or high. Confirm with yes)
C:X P:Y END
- + other yes Select next type of step
5.2.5 Process Step: END
C:Y P: X END Select and confirm End step

- + other yes
Cycle Y END ? Confirm end of programming for this cycle

no yes
Select number of cycles to be performed

Nr. of Cycles: 1
- + yes using these programmed steps (1 - 9)
Different Cycle? Select if a different cycle

no yes is to be defined
C:Z P:1 END Select steps for next cycle

- + other yes
Final Aspirate ?
Select if the microplate is to be aspirated
no yes at the end of the procedure
Select required aspirating time (1 - 20 sec)

Time: 4 sec
- + exit yes
Speed: 10mm/s
Select required aspirating speed (1 - 20 mm/s)
- + exit yes
Flat bottom wells Round bottom wells
Crosswise: NO Select if crosswise aspiration is to be used

- + exit yes
Run X:yyyyyyy
Instrument ready to perform defined
- + other yes washing procedure
5.3 Editing a Defined Program

To edit a defined program, proceed as described in the Defining a New Program
section and edit the parameters as required. If the program is locked it can not be
edited with the onboard software and the following error message is displayed:
Program locked
yes
The default programs on the Washer 430 are locked.
5.3.1 Changing the Type of Defined Process Step

To change the type for a defined process step, use the - and + keys to scroll to
the required process step number that is to be altered.
When the required process step number is displayed, press the other key to
scroll to the required type of process steps across the display.
When the required type of process step is displayed, press yes to confirm the
selection.
The instrument enters the programming procedure for the selected process step.
Note
If an existing process step has been changed to End, all previously defined
steps after this step in this cycle have now been deleted.
5.3.2 End Programming Procedure

To end the programming / editing procedure when all the required parameters
have been altered, use the - and + keys to scroll to the End program step.
C:X P:Y END
- + other yes
Where C:X is the cycle number and P:Y is the process step number for this cycle.
Press yes to confirm the selection of the End process step and the programmed
parameters for the End step are displayed.
When the parameters for the End step have been confirmed, the instrument
returns to the standby mode and the following message is displayed:
Run XX:yyyyyyy
- + other yes
The instrument is now ready to use the program, which has been edited.
5.4 Show Program

This submenu is used to display the parameters that have been defined for a
program.
The displayed parameter settings can not be altered in this submenu.
Show XX:yyyyyyy Select required program to

- + other yes be shown
Plate: X Selected plate type displayed

exit yes
Selected manifold type displayed

Manifold Type:8 (only the 8-way manifold is available
exit yes
for the Washer 430)
Disp Rate: X
Selected dispensing speed (1 - 5 or drip)
exit yes
Asp Rate: X Selected aspirating speed (1-5)

exit yes
Plate type
Round bottom Plate type Flat bottom
Crosswise: XX Selected setting for crosswise aspiration

exit yes
Sel. Strips: XXX Selected setting for strip selection

exit yes
Mode: xxxxxx Selected operating mode

exit yes (strip or plate)
C:1 P:1 XXXX Select required process step to be shown

- + Exit yes using - and + keys or press yes to step
through all steps and settings
Show XX:yyyyyyy
- + other yes
5.5 Clear Program

This submenu is used to clear a program from the instrument's memory. If the
program is locked it can not be cleared and the following error message is
displayed:
Program locked
yes
Clear XX:yyyyyyy Select required program to be cleared

- + other yes from instrument's memory
Clear XX:yyyyyyy Confirm that selected program is to

no yes be cleared
Clear XX:yyyyyyy Select next program to be cleared or

- + other yes required option in Mode Selection Menu
The function Clear ALL is available within the Clear Program option. This
function enables you to clear all of the programs (excluding those that are
locked!). The Clear ALL function is found before program 1 and after
program 30. Use the cursor keys to skip through the programs and once Clear
ALL is displayed press yes and all of the programs (with the exception of those
that are locked) will be cleared.
6. Adjust Menu
6. Adjust Menu
6.1 Introduction
The Adjust menu has the following options:
Plate Adjustment procedure for setting the parameters for up to 9

different types of microplates.
Prime Time Adjust the length of time that the Priming procedure is
performed.
Rinse Time Adjust the length of time that the Rinsing procedure is
performed.
Autorinse Adjust the time between the Autorinse rinsing cycles.
Kit Selection Only available if a kit is defined by computer software.
Date Set the date (only with the Barcode option).
Time Set the time (only with the Barcode option).
Sel. Manifold Set the manifold that is fitted to the instrument.
If the option Manifold Detection or Fill Verification is
enabled, this menu point does not exist. If either of these
options has been selected, then the manifold type will be
detected automatically.
Adj. Liq. Sens. Adjust Liquid Sensors
Adjustments can be made to the switch thresholds for the
bottles containing the wash, rinse and waste liquids.
6. Adjust Menu
Adjust Menu Confirm selection of Adjust menu

other yes
Plate: 1 Plate Type definition menu

- + other yes Use - and + keys to select required plate type
Plate Type definition procedure
Priming Time setting

Primetime:10 sec
- + other yes Use - and + keys to select required priming time
(5 - 99 sec.)
Rinsetime: 10sec Rinse Time setting

- + other yes Use - and + keys to select required rinsing time
(5 - 99 sec.)
AutoRinse: 1 h Automatic Rinsing Time setting

- + other yes Use - and + keys to select required time between
Automatic Rinsing procedures (1 - 24 hrs.)
Kit Select: Off This option is not available with

- + other yes this instrument
Date setting (option)

Date: ddmmmyyyy
Use d to change day setting
d m other y
Use m to change month setting
Use y to change year setting
Time: hh:mm xx Time setting (option)
h m other form Use h to change hour setting
Use m to change minute setting
Use Form to change from 12 to 24 hr format
Sel.Manifold: xx Manifold setting (optional)

- + other yes Use - and + keys to select fitted manifold
(only the 8-way manifold is available for the Washer 430)
If manifold detect option or fill verfication is enabled, this
setting can not be used
Adj. Liq Sens.:
Adjust Liquid Sensor (optional)
other yes
Using the - and + keys select the liquid sensor.

1 = Green (wash solution) For Option A / A & B
Liq. Sens. 1 2 = Gray (rinse solution) 3 = Blue
- + exit yes W = Red (waste) 4 = Purple
Press yes to continue the adjustment procedure.
Empty 1 XX 1 XX = displayed sensor value (does not directly correlate

exit yes to the volume of liquid in the bottles).
1 = value above limit
Press yes to store the currently displayed value as the
Empty 1 XX 0 limit.
exit yes
XX = displayed value 0 = values below limit
Press exit to continue the adjustment of other
Exit Adjust sensors.
other yes Exit Adjust menu
Run XX yyyyyyyy
- + other yes
6. Adjust Menu
6.2 Plate Type

This option is used to set the following parameters for the different types of
microplates. If a plate type is defined in a locked program it can not be edited and
the following error message is displayed:
Plate locked
yes
Before starting the adjustment procedure, insert the microplate to be
adapted into the plate carrier.
WARNING
MICROPLATE OR STRIP-PLATE USED. THIS ALSO APPLIES FOR ANY
PRE-DEFINED PLATE FILE, THAT WILL ALWAYS CONTAIN AVERAGE
PLATE PARAMETERS ONLY, THAT HAVE TO BE VERIFIED WITH THE
CORRESPONDING PLATE TYPE AND IF NECESSARY CORRECTED
BEFORE PUTTING THE WASHER 430 INTO USE.
IF THIS ADJUSTMENT PROCEDURE IS NOT PERFORMED PROPERLY,
THIS MIGHT RESULT IN HIGH LEVELS OF RESIDUAL VOLUME LEFT
PER WELL, AS WELL AS IN INSUFFICIENT WASHING OF THE WELLS
AND MAY SERIOUSLY AFFECT ASSAY PERFORMANCE.
Note
Suitable Microplates
standard defined by the Society of Biomolecular Screening. Other plate
types in 96-well format such as deep-well plates, PCR-plates and
filtration plates cannot be used with the Washer 430
Note
The Washer 430 allows to process flat-bottom or round-bottom strip-
well plates in 96-well format, typically used for EIAs, showing a similar
height and footprint as the 96-well microplates listed above.
• all strips have been properly inserted into the frame of the
strip-plate to give a level surface
6. Adjust Menu
6.2.1 Round Bottom Plates

other yes
Plate: X
Plate type definition menu
- + other yes Use - and + keys to select required platetype
(1 - 9)

890 ↵ ABCDE
(up to eight characters)
Bottomform Round Plate bottom form setting

- + exit yes Use - and + keys to select required bottom form
(flat or round)
Disp.Pos XXX Set dispensing position

- + exit yes Use - and + keys to move the plate support so
that the dispensing needles are in the correct position
Asp.Pos XXX Set aspirating position

that the aspirating needles are in the correct position
Set bottom aspirating position

Asp.Pos Botm XXX Use - and + keys to move the manifold so that the
- + exit yes
aspirating needles are in the correct position for
aspirating the bottom of the wells
Washpos Botm XXX

Set bottom washing position
- + exit yes Use - and + keys to move the manifold so that the
the bottom washing procedure
Washpos Ovfl XXX Set overflow washing position

the overflow washing procedure
Plate: X Select plate type to be defined
- + other yes
6. Adjust Menu
6.2.2 Flat Bottom Plates

other yes
Plate: X
Plate type definition menu
- + other yes Use - and + keys to select required plate type
(1 -9)

890 ↵ ABCDE
(up to eight characters)
Bottomform Flat Plate bottom form setting

- + exit yes Use - and + keys to select required bottom form
(flat or round)
Disp.Pos XXX Set dispensing position

that the dispensing needles are in the correct position
Asp.Pos 1 XXX Set aspirating position 1

- + exit yes Use - and + keys to move the plate support so that
the aspirating needles are in the correct position
Asp.Pos 2 XXX Set aspirating position 2

- + exit yes Use - and + keys to move the plate support so that
the aspirating needles are in the correct position
Only used if Crosswise Asp. is set to Yes.
Set bottom aspirating position

Asp.Pos Botm XXX
aspirating the bottom of the wells
Washpos Botm XXX

Set bottom washing position
the bottom washing procedure
Washpos Ovfl XXX Set overflow washing position

the overflow washing procedure
Plate: X Select plate type to be defined

- + other yes
6. Adjust Menu
6.2.3 Movement Diagrams

The following diagrams show how the plate carrier and manifold are moved for
the various settings.
Dispense Position
Instrument Front Instrument Front
Aspirate Position (1)
Position to Edge Position to Centre
Aspirate Position (2) Flat Bottom Wells Only
Instrument Front
Aspirate Bottom Position
Bottom Wash Position
Overflow Wash Position
6. Adjust Menu
6.3 Adjust Liquid Sensors

Note
Every Washer 430 is delivered with
the liquid sensor system pre-calibrated.
Calibration is only necessary if Option A is refitted in the instrument.
Caution
This procedure must be performed when Option A is
refitted in the instrument.
This procedure allows a warning level to be determined. This informs the

user when the level of liquid in the bottles is too low or too high.
When the level of liquid in the liquid bottles 1 & 2 (and optionally 3 & 4) is below
600ml and the level of the liquid in the waste bottle is above 2000ml the
instrument will emit an acoustic signal to alert the user.
Fill the bottles before performing the adjustment procedure. The bottles marked
green (wash solution) and gray (rinse solution) should be filled with 600ml of
water and the bottle marked red (waste) should be filled with 2000ml of water. For
Option A and option A & B use similar liquid volumes (600 ml) for the purple and
blue bottles. Place all bottles on the corresponding colored bottle holders. Go to
the Adjust menu and select Adj. Liq. Sens.
Adj. Liq. Sens.: Press yes to start the adjustment procedure

other yes
Using the - and + keys select the liquid sensor.
1 = Green (wash solution) For Option A / A & B
2 = Gray (rinse solution) 3 = Blue
Liq. Sens. 1 W = Red (waste) 4 = Purple
- + exit yes
Press yes to continue the adjustment procedure.
Empty 1 XX 1 XX = displayed sensor value (does not directly correlate

exit yes to the volume of liquid in the bottles).
1 = value above limit
Press yes to store the currently displayed value as the
Empty 1 XX 0 limit.
exit yes
XX = displayed value 0 = values below limit
Press exit to continue the adjustment of other
sensors.
Use the cursor keys to repeat this

procedure for the gray (rinse solution)
and red (waste) bottles. See overleaf for
more details.
6. Adjust Menu
Liquid Sensors 1 - 4
When liquid sensor 1, 2, 3 or 4 is selected, the following message is displayed:
Empty S XX Y
exit yes
• S = sensor number
• XX = displayed value (does not directly correlate to the volume of liquid in the bottles).
• Y = limit (0 = values below limit, 1 = values above limit)
Ensure that bottle 1, 2, 3 or 4 are filled with 600ml of water and are placed on the
corresponding green or gray bottle holder.
Press yes to confirm the value (volume).
When the sensor detects a volume less than this entered volume, it will display
an error message either before starting the test procedure or after the procedure
has been completed.
When the value has been set, press exit and the following message is displayed:
Liquid Sens. 1
- + exit yes
Use the < and > keys to set the next required sensor (1 - 4 or W).
Waste Sensor
When the waste sensor is selected, the following message is displayed:
Full W XX Y
exit yes
• W = waste sensor
• XX = displayed value (does not directly correlate to the volume of liquid in the bottles).
• Y = limit (0 = values below limit, 1 = values above limit)
Ensure that the waste bottle is filled with 2000ml of water and is placed on the
corresponding red bottle holder.
Press yes to confirm the value (volume).
When the sensor detects a volume greater than this entered volume, it will
display an error message either before starting the test procedure or after the
procedure has been completed.
When all the sensors have been set, press exit and the following message is
displayed:
Adj.Liq.Sens.:
other yes
Press other to proceed to the next option.

7.1 Cleaning Procedures
WARNING
ALL PARTS OF THE INSTRUMENT THAT COME INTO CONTACT WITH
POTENTIALLY INFECTIOUS MATERIAL MUST BE TREATED AS
POTENTIALLY INFECTIOUS AREAS. IT IS ADVISABLE TO WEAR
PROTECTIVE POWDER-FREE GLOVES WHEN PERFORMING THE
CLEANING PROCEDURE AND ALSO WHEN MAKING ADJUSTMENTS TO
THE INSTRUMENT.
The most important cleaning procedure for this instrument is to rinse the liquid
system with distilled water before the instrument is left to stand or switched off at
the end of each day.
The manifold should be removed and thoroughly cleaned at least once a month
or if one or more of the needles are blocked.
Caution
If the manifold is not rinsed, the needles will become blocked.
This will result in the manifold either needing expensive repairs
or it could become completely ruined.
7.1.1 Outer Surface of the Instrument Housing

The outer surface of Washer 430 may be cleaned periodically using a tissue
moistened with a mild detergent solution.
7.1.2 Display Window Cover

The display window cover on the alphanumeric display may be cleaned
periodically using a tissue moistened with a mild detergent.
7.1.3 Cleaning Pumps and Tubes

To clean the pumps and tubes perform Rinse-Night on a daily basis. (See chapter
7.5 Rinse Modes).
7.1.4 Manifold Cleaning

The most important cleaning procedure for this instrument is to rinse the wash
system before the instrument is left to stand or switched off at the end of each
day.
Caution
If the manifold is not rinsed, the needles will become blocked.
This will result in the manifold either needing expensive repairs
or it could become completely ruined.
WARNING
All parts of the instrument that come into contact with potentially
infectious material must be treated as potentially infectious areas. It is
advisable to wear protective powder-free gloves when performing the
cleaning procedure and also when making adjustments to the instrument.
The manifold can be cleaned using:

• The supplied cleaning needles (accessory box)
Carefully push the cleaning needles into the aspirating and dispensing
needles.
The small cleaning needle is for the dispensing needles and the large
cleaning needle is for the aspirating needles.
Rinse the manifold block with distilled water to ensure that all particles have
been removed.
• A gentle ultrasonic bath of warm distilled water for 15 minutes.
• Autoclaving (max. 130 °C , max. five times)
Note
Autoclaving is only intended for use with a manifold that is not fitted with
the Fill Verification option. Manifolds with the Fill Verification option
should only be cleaned in an ultrasonic bath. If additional cleaning is
required, use a small brush. Do not use autoclaving.
Note
For instruments fitted with the Fill Verification Option, the underside of the
manifold must also be cleaned daily to avoid Fill Function Errors (see
chapter 4.5.4 Instrument Fitted with Fill Verification (Option B).
Should it become necessary to take the manifold apart (possibly infectious!) take
care to insert all seals properly when reassembling the manifold.
Twisted or missing seals may result in a leaking manifold!
After the manifold has been cleaned, reconnect the tubing and replace it into the
instrument. Tighten the manifold into place using the two screws, ensure that the
screws are tight.
When the manifold has been refitted, switch the instrument on and perform the
priming procedure using distilled water.
7.1.5 Cleaning Waste Bottles
WARNING
BEFORE CLEANING THE WASTE BOTTLES EMPTY THEM ACCORDING
TO DISPOSAL REGULATIONS (SEE CHAPTER 7.7.3 DISPOSAL OF
OPERATING MATERIAL).
THE BOTTLES MUST BE CLEANED REGULARLY DEPENDING ON THE
APPLICATIONS, USING A MILD DETERGENT.
Note
It is advisable to wear disposable powder-free gloves
when handling waste bottles.
7.1.6 Liquid or Foam Spills

Spilling of liquid or foam may occur when the Washer 430 is operated improperly
such as:
• Used microplate not matching installed manifold
• Position of the strips in the strip-plate do not match the positions defined in
the program used for processing
• Plate parameters not properly adjusted
• Waste bottle not emptied when liquid level or foam level reaches maximum
filling level
• No anti-foaming agent used with wash buffers showing strong tendency to
foam
WARNING
MAKE SURE THAT THE LIQUID LEVEL OF THE WASTE BOTTLE IS
WARNING
ALWAYS SWITCH-OFF WASHER 430 BEFORE REMOVING ANY KIND
SPILLS ON THE INSTRUMENT. IF THE SPILL OCCURS IN THE
INSTRUMENT A SERVICE TECHNICIAN IS REQUIRED.
WARNING
WHEN CLEANING SPILLS ALWAYS WEAR DISPOSABLE POWDER-
FREE GLOVES AS WELL AS PROTECTIVE GLASSES AND CLOTHING.
All spills (liquid or foam) have to treated as potentially infectious. Therefore
always wear protective powder-free gloves and protective clothing when cleaning
up a spill. Additionally, all resulting waste has to be treated as potentially
infectious and the disposal has to be performed according to the information
given in chapter 7.7.3 Disposal of Operating Material.
Always remove spills immediately after they have occurred. Use paper tissue to
soak-up spills and wipe-surface dry. Carefully disinfect surface were spill has
occurred according to the disinfection procedure in chapter 7.6.1 Disinfection
Procedure.
7.2 Manifold Removal
WARNING
AFTER THE INSTRUMENT HAS BEEN USED,
THE MANIFOLD MAY BE INFECTIOUS.
BEFORE THE MANIFOLD IS REMOVED IT MUST BE THOROUGHLY
DISINFECTED AND OBSERVE PROPER LABORATORY SAFETY
PRECAUTIONS, SUCH AS WEARING PROTECTIVE CLOTHING AND USING
APPROVED LABORATORY SAFETY PROCEDURES.
The manifold should be removed and thoroughly cleaned at least every 6 months
or if one or more of the needles are blocked.
Lift the manifold arm and remove the two screws that hold the manifold onto the
holder.
Fig. 7-1 The Manifold
Screws
When the two screws have been removed, carefully pull the manifold away from
the holder.
Carefully pull the tubing off the connectors on the rear of the manifold.
Fig. 7-2 The Rear of the Manifold
Dispensing Tube
Locating Pin
Locating Pin
Aspirating Tubes
Manifold Indicator Type
7.2.1 Replacing the Manifolds

The Washer 430 is delivered with the manifold already installed.
The instrument can be used with the following types of manifolds:
• Standard (8 way manifold)
• Fill verification Factory installation required
The manifold is fitted to the instrument using the following procedures:

1. Lift the manifold arm.
2. Carefully fit the manifold onto the manifold arm and ensure that the locating
pins are correctly inserted through the holes in the plate.
3. If the manifold detection option is fitted, ensure that the manifold type
indicator is correctly fitted between the manifold type detector switches.
4. Tighten the manifold into place using the two screws.
5. Fit the dispensing tube onto the top connector on the rear of the manifold.
6. Fit the aspirating tubing (marked with red rings) onto the two bottom
connectors on the rear of the manifold.
7. If the Fill Verification option is fitted, connect the small ribbon cable onto
the connector block on the left hand side of the circuit board in the manifold
arm.
Manifold for the Standard Instrument

Dispensing Tube Connectors (blue)
Locating Pin
Locating Pin
Manifold Type Indicator Aspirating Tube Connectors (red)
Manifold for Instrument with Fill Verification

Fig. 7-3 Rear View of the Manifold
Aspirating Tube Connectors Dispensing Tube Connector
red blue
Locating Pin
Locating Pin
Manifold Type
Indicator
Ribbon Cable
Aerosol Tube Connectors yellow
Ribbon Cable
connectors
1
For a 8 or 12 2
manifold only use
connector 2
8. When all the required tubing and ribbon cable have been connected, lower
the manifold arm.
7.2.2 Replacing the Plate carrier
WARNING
THE INSTRUMENT.
The instrument is delivered with the plate carrier already installed. If the plate
carrier is removed, for example: for cleaning, it can be replaced using the
following procedure:
1. Lift the manifold arm
2. Slightly tilt the plate carrier towards the right.
3. Carefully slide the plate carrier with the waste sink at the rear over the
guide bar.
GUIDE BAR
WASTE SINK
The plate carrier must be slightly tilted so that it goes over the pin that activates
the automatic microplate centering mechanism.
To reinstall the plate carrier follow the procedure listed below:
1. Push the plate carrier so that it is fully inserted into the instrument.
2. Lower the plate carrier.
3. Lower the manifold arm.
7.3 Replacing the Main Fuses
WARNING
TO PREVENT THE RISK OF FIRE, THE MAIN FUSES SHOULD ONLY BE
REPLACED WITH THE SAME TYPE AND RATING OF FUSE.
The following steps must be performed to replace the Main Fuses, which are
located by the power cable connection, in the rear panel of the instrument.
1. Switch off the instrument and unplug the power cord.
Fig. 7-4 The Fuse Compartment
Fuse
Compartment
0
l
2. Open the fuse compartment, by inserting a screw driver into the slot in the
right hand side of the compartment. Remove the compartment out and to the
right.
Fig. 7-5 The Fuses
Fuses
0
l
3. Remove the fuses and replace them.

4. Ensure that the fuses have the correct rating.
T 1,6 A / 250 V (slow blow)
5. Replace the fuse compartment.
6. Reconnect the power cord and switch the instrument on
WARNING
IF THE FUSE CONTINUES TO BLOW, PLEASE CALL FOR SERVICE.
7.4 Preventive Maintenance Plan
WARNING
THE INSTRUMENT.
7.4.1 Daily
• Perform Rinse Night with distilled water.
7.4.2 Weekly
• Perform Rinse Night with distilled water.
• Check the liquid filter(s) in wash bottle(s) for particles and rinse liquid filter(s)
with water.
7.4.3 Every Six Months

• Clean the plate carrier guide bar with 70% ethanol.
• Check the centering mechanism of the plate carrier and clean if necessary
with 70 % ethanol.
• Clean the Manifold aspirating and dispensing needles using the cleaning kit
supplied with the instrument.
7.4.4 Yearly (Service Engineer Required)

• Save the customers specific programs using the WinWash Clinical software.
• Disinfect the instrument. (See 7.6 Instrument Disinfection)
• Replace all aspirating and dispensing tubes.
• Replace the liquid filter(s) in the wash bottle(s).
• Replace the manifold sealing and clean the inner parts of the manifold.
• Check the aspirating pump and replace if necessary.
• Recalibrate the dispensing pump.
(See Washer 430 Technical Manual - chapter 3)
• Download the specific saved programs to the washer.
7.5 Rinse Modes

There are three rinse modes to flush the liquid system and prevent needle
blockages. They should be carried out when the instrument is left to stand or is
switched off at the end of operation.
The Rinse modes are:
Rinse Day
Rinse Night
Rinse Auto
7.5.1 Rinse: Day

This procedure is used to rinse the wash solution system if the instrument is to be
left standing for a longer time. Alternatively it can be used to rinse the wash
solution system after the instrument has been left for a long time.
With this procedure you can use either distilled water or wash solution.
If the instrument is left to stand for 1 hr to 1/2 day, wash solution can be used, for
longer times use distilled water.
Rinse: Day
- + other yes
Select and confirm Day Rinse procedure
Day: CH1 Select required channel (1 - 4)

- + exit yes
Rinse Solution ? Confirm rinsing solution and tube in bottle

exit yes
Waste Bottle OK? Confirm waste bottle is empty

exit yes
Rinse ChX YY sec Wash system rinsed for set rinse time
Stop
The waste sink is filled with solution and

the manifold is lowered into it
Soaking Manifold left in solution until

Stop Stop key pressed
Run XX:yyyyyyy Standby mode

- + other yes Washing Procedure can be performed
7.5.2 Rinse: Night

This procedure is used to thoroughly rinse the wash solution system and leave
the manifold soaking in the waste sink full of solution at the end of operation. This
procedure requires distilled water.
Rinse: Night Select and confirm Night Rinse procedure
- + other yes
Night: CH1 Select required channel (1 – 2 or 1 – 4 if

- + exit yes additional channels are installed)
Rinse Solution ?
exit yes Confirm rinsing solution and tube(s) in bottle(s)
Waste Bottle OK? Confirm waste bottle is empty

exit yes
Stop
The waste sink is filled with distilled water and

the manifold is lowered into it.
Soaking xx sec Manifold left in solution for set soak time (1 min)
Stop
Stop
The waste sink is filled with distilled water and the

manifold is lowered into it.
Soaking Manifold left in solution until Stop key pressed

Stop
Run XX:yyyyyyy Standby mode

- + other yes Washing procedure can be performed
7.5.3 Rinse: Auto

This procedure is the same as the Rinse: Day procedure, except that the rinsing
procedure is automatically repeated after a set repeat time. The repeat time can
be set in the Adjust menu between 1 and 24 hrs.
7.6 Instrument Disinfection
WARNING
THE INSTRUMENT.
It is very important that the instrument is thoroughly disinfected before it is
removed from the laboratory or any servicing is performed on it.
Before the instrument is returned to the distributor for servicing, it must be
disinfected and a disinfection certificate completed by the operating authority.
If a disinfection certificate is not supplied, the instrument may not be accepted by
the servicing center or it may be held by the customs authorities.
7.6.1 Disinfection Procedure
Caution
Before starting the disinfection procedure use a rinse procedure
(Rinse Day) with distilled / deionized water (lab quality) to flush the system.
If the laboratory has no specific disinfection procedure, the following procedure

should be used to disinfect the instrument.
The instrument should be disinfected using one of the following solutions:
Lysetol Manufacturer: Schülke & Mayr GmbH.
Aseptisol Manufacturer: Bode Chemie Hamburg
If neither of these solutions are available 70% ethanol should be used as an
alternative.
WARNING
THE DISINFECTION PROCEDURE SHOULD BE PERFORMED BY
AUTHORIZED TRAINED PERSONNEL IN A WELL VENTILATED ROOM
WEARING DISPOSABLE POWDER-FREE GLOVES AND PROTECTIVE
GLASSES AND CLOTHING.
Please note that the disinfectant can influence the performance of your
instrument if it comes into contact with the electronics!
The following procedure should be used to disinfect the instrument.

1. Wear protective powder-free gloves, protective glasses and protective
clothing.
2. Prepare an autoclaveable bag for all disposables used during the disinfection
procedure, label it with autoclave tape and put it in the autoclave or follow the
procedure defined in chapter 7.7.3 Disposal of Operating Material.
3. Prime the liquid system with disinfectant. Perform the Rinse - Night
procedure with disinfectant solution and stop the procedure after five hours.
4. Switch off the instrument and disconnect the instrument from the mains
power supply.
5. Disconnect the instrument from any accessories that are used for example:
Liquid Sensor System, computer, etc. Accessories that should be shipped
together with the instrument have to be included in the disinfection
procedure.
6. Carefully spray the disinfectant solution (or use a disposable soft tissue
paper towel soaked in the disinfectant) on all outer surfaces of the
instrument.
7. After a minimum contact time of 10 minutes, repeat step 6 of this procedure.
8. Wipe dry the outer surfaces of the instrument.
9. Pack the instrument and its accessories.
10. Disinfect your hands and clean them with a mild detergent.
11. Complete a disinfection certificate and attach it to the outside of the
box so that it is clearly visible. (See below for an example of the
disinfection certificate).
The following label MUST be completed by the operating authority and attached
to the top of the package in which the instrument is returned, before sending the
instrument to the servicing center for repair.
I declare that the instrument in this package has never been exposed to any
hazardous biological material or that it has been decontaminated or
disinfected to remove or inactivate any biological material which could be
dangerous to the service personnel.
Name:...................................................................................................................
Firm: .....................................................................................................................
Address: ...............................................................................................................
.............................................................................................................................
.............................................................................................................................
Country:................................................................................................................
Signature:.............................................................................................................
7.7 Disposal of Instrument

7.7.1 Introduction
This chapter gives instructions on how to lawfully dispose of waste material
accumulating in connection with the Washer 430.
Caution
Observe all federal, state and local environmental regulations.
7.7.2 Disposal of Packing Material

According to Directive 94/62/EC on packaging and packaging waste, the
manufacturer is responsible for the disposal of packing material.
Returning Packing Material

If you do not intend to keep the packing material for future use, e.g. for transport
and storage purposes:
Return the packaging of the product, spare parts and options via the field service
engineer to the manufacturer.
7.7.3 Disposal of Operating Material
WARNING
CHEMICAL AND BIOLOGICAL HAZARDS CAN BE ASSOCIATED WITH
THE WASTE MATERIAL (MICROPLATE) OF THE PROCESS RUN ON THE
WASHER 430.
TREAT THE USED MICROPLATE, WASTE BOTTLE, WASTE SINK ON
THE PLATE CARRIER, DISPOSABLES AND ALL SUBSTANCES USED,
IN ACCORDANCE WITH GOOD LABORATORY PRACTICE GUIDELINES.
INQUIRE ABOUT APPROPRIATE COLLECTING POINTS AND APPROVED
METHODS OF DISPOSAL IN YOUR COUNTRY, STATE OR REGION.
7.7.4 Disposal of the Washer 430

Please contact your local bioMérieux service representative before disposing of
the instrument.
Caution
Always disinfect the instrument before disposal.
Pollution degree 2 (IEC/EN 61010-1)

Method of Disposal Contaminated Waste
WARNING
DEPENDING ON THE APPLICATIONS, PARTS OF THE WASHER 430
MAY HAVE BEEN IN CONTACT WITH BIOHAZARDOUS MATERIAL.
• MAKE SURE TO TREAT THIS MATERIAL ACCORDING TO THE
APPLICABLE SAFETY STANDARDS AND REGULATIONS.
• ALWAYS DECONTAMINATE ALL PARTS BEFORE DISPOSAL.
8. Trouble Shooting
8. Trouble Shooting
8.1 Introduction
Error Description Possible Causes See
Chapter
Incorrect Plate Plate parameters are not defined 6
Position (Adjust Menu)
Program not defined No test defined in the instrument memory 5
Program too Big If more than 60 process steps have been 5

defined
Wrong Manifold Manifold not correctly defined in Adjust Menu 6
Display and LED Defective fuse 7.3

dark
No or incorrect Dispensing needles blocked 7

dispensing
No or incorrect Aspirating needles blocked 7

aspirating
Manifold Arm Error The manifold arm does not move up and down 4
Index
Index
A Error Messages ........................................... 4-10
Adjust Liquid Sensors.................................... 6-7 F

Adjust Menu................................................... 6-1
Fill Verification ............................................... 4-8
Aspirate.......................................................... 5-3
Firmware Update......................................... 2-11
Aspirating Positions ..................................... 2-16
Flat Bottom Plates ......................................... 6-5
B
I
Bottom Washing .......................................... 2-15
Installation ..................................................... 3-4
Buffers
Installation Procedure.................................... 3-1
Conductance of some Standard Wash Buffers
Installing the Instrument ................................ 3-4
................................................................ 4-9
Instrument
C Description................................................. 2-4
Disinfection .............................................. 7-13
Cleaning......................................................... 7-1 Disposal ................................................... 7-15
Cleaning Pumps and Tubes .......................... 7-1 Installation.................................................. 3-4
Conductance of some Standard Wash Buffers4- Options .................................................... 2-11
9 Procedure when Left Switched On .......... 4-15
Connecting the Solution Tubes ..................... 3-4 Procedure when Switched Off ................. 4-15
Connections Specifications............................................. 2-2
Diagrams.................................................... 2-6 Switching OFF ......................................... 4-15
Rear Panel ................................................. 2-5 Switching ON ............................................. 4-1
Instrument Options
D Option A................................................... 2-11
Option B................................................... 2-11
Dilution Washing.......................................... 2-15
Disinfection .................................................. 7-13 K
Dispense........................................................ 5-4
Display Window Cover .................................. 7-1 Keyboard and Display Unit............................ 2-4
Disposal ....................................................... 7-15
Operating Material ................................... 7-15 L
Packing Material ...................................... 7-15
Liquid Sensors
Download software ...................................... 2-11
Connection................................................. 3-5
E Liquid Sensors 1 - 2 ...................................... 6-8
Liquid Spills ................................................... 7-4
END ............................................................... 5-5 Liquid System.............................................. 2-13
End of Operation ......................................... 4-15
End Programming Procedure ........................ 5-6 M
Error
Main Fuses
Fill Aspiration ............................................. 4-8
Replacing................................................... 7-9
Fill Aspiration Error .................................. 4-14
Maintenance................................................ 7-10
Fill Communication Error ......................... 4-14
Maintenance and Cleaning ........................... 7-1
Fill Dispense .............................................. 4-8
Manifold
Fill Function................................................ 4-9
Cleaning..................................................... 7-2
Fill Function Error..................................... 4-14
for Instrument with Fill Verification............. 7-7
Liquid Bottle Empty .................................. 4-12
Removal..................................................... 7-5
Liquid Not Dispensed............................... 4-13
Replacing................................................... 7-6
Manifold Arm Error................................... 4-13
Manufacturer ................................................... 2
Program not defined ................................ 4-10
Mode Selection Menu.................................... 4-2
Transport Error......................................... 4-10
Movement Diagrams ..................................... 6-6
Waste Bottle Full at Start ......................... 4-11
Waste Bottle Full during Procedure ......... 4-11 N
with Fill Verification .................................. 4-13
with Liquid Sensor.................................... 4-11 New Program
with Manifold Detection Sensors ............. 4-13 Defining...................................................... 5-2
Wrong Manifold........................................ 4-10
2004-12 Instructions for Use for Washer 430 No. 45610087 Rev. No. 2.2 Index
Index
O S
Overflow Washing ....................................... 2-15 Safety Precautions ........................................ 1-1
Soak .............................................................. 5-4
P Software Features ....................................... 2-14
Software Menus .......................................... 2-14
Packing Material
Solution Tubes
Disposal ................................................... 7-15
Connecting................................................. 3-4
Returning ................................................. 7-15
Strip Mode ................................................... 2-15
Plate carrier
Strips
Replacing ................................................... 7-8
Washing individually .................................. 4-7
Plate Mode .................................................. 2-15
Switching OFF the Instrument..................... 4-15
Plate Type ..................................................... 6-3
Symbols............................................................ 4
Pollution degree........................................... 7-16
Power Connections ....................................... 3-6 T
Power Requirements ..................................... 3-3
Priming........................................................... 4-3 Trouble Shooting ........................................... 8-1
Process Step
Changing.................................................... 5-6 U
Program
Unpacking Procedure.................................... 3-2
Clear........................................................... 5-8
Editing ........................................................ 5-6 W
Show .......................................................... 5-7
Programming Procedure ............................... 5-1 Wash ............................................................. 5-3
Ending ........................................................ 5-6 Wash Individually Selected Strips
Procedure .................................................. 4-7
R Wash Procedure
Performing ................................................. 4-5
Rear Panel Connections................................ 2-9
Washing Modes........................................... 2-15
Rinse Modes.......................................2-17, 7-11
Waste Sensor................................................ 6-8
Auto.......................................................... 7-12
Day........................................................... 7-11
Night......................................................... 7-12
Round Bottom Plates..................................... 6-4
Index Instructions for Use for Washer 430 No. 45610087 Rev. No. 2.2 2004-12

Operating Manual Washer 430

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Operating Manual Washer 430

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ANSI American National Standards Institute

REF Reference Number/ Order Number

SBS Society for Biomolecular Screening

T Träge (Slow Blow Fuse)

TYPE Name and type of instrument

7. Maintenance and Cleaning

2.2 Instrument Specifications

2.3 Instrument Description

Keyboard and Display Unit

Fig. 2 The Keyboard and Display Unit

Key 1 Key 2 Key 3 Key 4

2.3.1 Rear Panel Connections

figure 2-3 Option A / Option A & B:

ON/OFF Inlets for channels 1 - 4

Bottle Holder with Serial RS 232 for Waste

All connected devices must be approved and listed with regulations

2.3.2 Connection Diagrams

Washer 430 / Option A / Option A & B

Bottle holder 1 1 Green Wash liquid 1

Bottle holder 2 3 Blue Wash liquid 3

Fig. 5 Washer 430 / Option B

Bottle Holder with liquid

Fig. 6 Washer 430 and Option A / Option A & B

Input Input Input Input

Marked green Maximum

grey red yellow purple

Bottle holders with

2.3.3 Rear Panel Connections

All connected devices must be proved and listed with regulations

2.4 Instrument Options

Option A : Upgrade from 1 inlet channel to 4 inlet channels

Field upgrade for option A is available from serial

Option B : Fill Verification option (8 channel manifold only) is

Please note that it is possible to combine option A

Firmware Update Consisting of the Download software, the latest version of

Article Number Description

45610067 Fuse 1.6A, 5 x 20 MM, SLOWBLOW (Net Entry)

45610073 Tubing, Prime Valve (5 / Package)

45610077 Tubing Set, External

45610078 Plate Carrier Complete

45610090 Cable Interface, Washer to PC

45610105 Plate Cover, Manifold

45610107 Filter, Inlet Tubing (5 / Package)

45610255 Tube Support

45610257 Cable Interface, Washer to Bottle Holder

45610084 Maintenance Kit

45610085 Aspirate Needle Cleaning

45610086 Dispense Needle Cleaning

45610087 Instructions for Use for Washer 430

45610088 Download-QcCol Disks

45610126 Instructions for Use for WinWash Clinical Washer 430

45610140 Plate, Greiner F (For QC)

45610156 Operating Manual for Download & QcCol

45610158 WinWash Clinical, Washer 430 Software CD-ROM

45610071 Manifold, 8 Channel, Standard

45610081 Bottle, Wash (Green)

45610082 Bottle, Waste (Red)

45610083 Bottle, Rinse (Gray)

2.4.1 Liquid System Diagrams