University of Yangon

Department of Chemistry

Research Proposal For 2023 B.Sc

Title - Determination of Amino Acids Content in crackers

Supervisor - Dr Yee Mun Than (Associate Professor, Department of
Chemistry)
Candidate -Mg Paing Soe Htoon
Roll No - 4-Chem-12
Field of Study - Analytical Chemistry
Date - 26.6.2023

Aim
> To study the analysis of amino acids in crackers

Objectives
• To collect the cracker samples from local markets.
• To prepare the reagent solutions (ninhydrin, phosphate buffer and
sample solutions)
• To prepare the standard solution of glycerine
• To measure the wavelength of maximum absorption for standard
glycerine using the UV-visible spectrophotometer
• To construct a standard calibrations curve for glycerine
• To determine the amino acids in cracker samples by spectrophotometric
method
 (a) (b) (c)
Figure 1: The different brands of crackers (a) Myanbisco crispy chicken crackers
(b) Julie’s golden crackers and (c) Hup Seng cream crackers

Materials and Methods

• The cracker samples will be purchased from local market.
• The samples will be ground into powder in an electric blender and will be
stored in airtight container.
• The ninhydrin reagents, phosphate buffer, standard glycerine solution and
sample solution will be prepared.
• The wavelength of maximum absorption for standard glycerine using the
UV-visible spectrophotometer will be measured.
• A standard calibration curve for glycerine will be constructed.
• The amino acids content cracker samples will be determined by
spectrophotometric method.