University of Yangon

Department of Chemistry

Research Proposal For 2023 B.Sc.

Title - Determination of Amino Acids Content in Biscuits

- Dr Yee Mun Than (Associate Professor, Department
Supervisor
of Chemistry)
Candidate -Ma Phyu Hnin Khaing
Roll No - 2H-Chem-13
Field of
- Analytical Chemistry
Study
Date - 26.6.2023

Aim
> To study the analysis of amino acids in biscuits

Objectives
• To collect the sample from local markets.
• To prepare the reagent solutions (ninhydrin, phosphate buffer and
sample solutions)
• To prepare the standard solution of glycerine
• To measure the wavelength of maximum absorption for standard
glycerine using the UV visible spectrophotometer
• To construct a standard calibrations curve for glycerine
• To determine the amino acids in biscuit samples by
spectrophotometric method
 (a) (b) (c)

Figure 1: The different brands of dry cakes (a) Ba Yin(b) Myint Myint Khin and
(c) Sar Kaung

Materials and Methods

• The biscuit samples will be purchased from local market.
• The samples will be ground into powder in an electric blender and will be
stored in airtight container.
• The ninhydrin reagents, phosphate buffer, standard glycerine solution and
sample solution will be prepared.
• The wavelength of maximum absorption for standard glycerine using the
UV-visible spectrophotometer will be measured.
• A standard calibration curve for glycerine will be constructed.
• The amino acids content biscuit samples will be determined by
spectrophotometric method.