BRANCH INDUSTRIAL BIOTECHNOLOGY

COURSE
GENOMICS AND PROTEOMICS SEMESTER
CODE
22BPE$13

PREREQUISITES CATEGORY L T P C

PE 3 0 0 3

Course 1. Provide basic knowledge of genomes and proteomes.

Objectives 2. Introduce relevant tools for the analysis of genomes.
3. Describe methodologies of genomic and proteomic techniques.
OVERVIEW OF GENOMES OF BACTERIA,
UNIT – I 9 periods
ARCHAEA AND EUKARYOTA
Genome organization of prokaryotes and eukaryotes, Gene structure of Bacteria, Archaea
and Eukaryotes, Minimal cell Genome, Human genome project, Introduction to functional
and comparative genomics.
UNIT – II MAPPING TECHNIQUES 9 periods
Cytogenetic mapping, Radiation hybrid mapping, Fish-STS mapping, SNP mapping, Optical
mapping. Linking and jumping of clones, Gap closure, Pooling strategies, Electronic PCR,
Automation in Genome sequencing-Next Generation Sequencing.
UNIT – III FUNCTIONAL GENOMICS 9 periods
Gene finding, Annotation of genome – experimental and computational approach. ORF
and functional prediction, Transcriptomics (RNA sequencing) & analysis of transcriptomic
data, high-throughput analysis of gene expression, DNA microarrays and expression
profiling
UNIT – IV PROTEOMICS TECHNIQUES 9 periods
Protein level estimation-Edman protein microsequencing, Protein cleavage, 2D gel
electrophoresis, metabolic labelling. Detection of proteins on SDS gels. Mass spectrometry
principles of MALDI-TOF, Fourier Transform Ion Cyclotron Resonance Mass
Spectrometer, Orbitrap Mass Analyzer, Tandem MS, Peptide mass fingerprinting.
UNIT – V PROTEIN PROFILING 9 periods
Large-scale protein profiling using proteomics, Protein-protein interactions, Functional
Proteomics Protein microarrays, proteome database. Bioinformatics-based tools for
analysis of proteomics data.
Contact Periods: 45
Lecture: 45 Periods Tutorial: 0 Periods Practical: 0 Periods Total: 45 Periods
Full Time B.E. 2022 Regulations 1/2

TEXT BOOKS:

1 Arthur Lesk., “Introduction to Genomics”, 3rd Edition, Oxford University Press, 2017.

2 Richard Twyman, Ph.D Cfe, George A, Principles of Proteomics”, 2nd Edition, Garland
Science, 2013.
3 Nawin C. Mishra, Günter Blobel, “Introduction to Proteomics: Principles and Applications”,
1st Edition, Wiley, 2010.

REFERENCES

1 Conard, Edward. “Genomics”. Apple Academics, 2010

2 Liebler, ‘Introduction to Proteomics’, Humana Press, 2002

3 T.A Brown, ‘Introduction to Genetic: A molecular Approach’, Garland Science, Taylor and
Francis, 2012
4 R.M.Twyman, S.B. Primrose, ‘Principle of Genome Analysis and Genomics’, Wiley Blackwell
Publications, 2007.

COURSE OUTCOMES: Bloom’s

Taxonomy
On completion of the course, the students will be able to: Mapped
CO1 Understand the basic structure and organization of genomes of K2
Prokaryotes
CO2 Understand the basic structure and organization of genomes of Eukaryotes K2
CO3 Have insight on basic organization of proteomes. K2
CO4 Analyze proteomes and genomes using the relevant tools. K3
CO5 Get familiarize with the principles of the methodologies of genomic K3
and proteomic

a) Course Articulation Matrix

PO PO PO PO PO PO PO PSO
COs/POs PO1
2
PO3 PO4
5
PO6
7
PO8
9 10 11 12 1
PSO2
CO1 L L - - - - - - - - - - H L
CO2 L L - - - - - - - - - - H L
CO3 L L L - - - - - - - - - H L
CO4 L L - - - - - - - - - - H L
CO5 L L - - - - - - - - - - H L
22BPE$13 L L L - - - - - - - - - H L
L – Low, M – Moderate, H – High

Prepared by Checked by Head of the Department

Full Time B.E. 2022 Regulations 2/2